strain atcc25418  (ATCC)

Journal: Microbiology Resource Announcements

Article Title: Complete genome sequence of Trinickia caryophylli ATCC25418

doi: 10.1128/mra.00929-24

Figure Lengend Snippet: The results of the genome sequence of T. caryophylli ATCC25418

Article Snippet: Strain ATCC25418 was obtained from the American Type Culture Collection in 2023.

Techniques: Sequencing

Journal: Scientific Reports

Article Title: Biotechnology methods for succession of bacterial communities in polychlorinated biphenyls (PCBs) contaminated soils and isolation novel PCBs-degrading bacteria

doi: 10.1038/s41598-022-23886-3

Figure Lengend Snippet: Taxonomic identification of single phylotypes found in the DNA-based SSCP fingerprints shown in Supplementary Fig. S1 A. online

Article Snippet: 24 , KZ7 , β Protobacteria , Burkholderia caryophylli ATCC 25,418 , AB021423.

Techniques: Sequencing, Bacteria

Journal: Nature Communications

Article Title: A pathway for chitin oxidation in marine bacteria

doi: 10.1038/s41467-022-33566-5

Figure Lengend Snippet: a Growth of chitinolytic Pseudoalteromonas spp. in the minimal medium supplemented with 0.2% (w/v) GlcNAc1A. The y -axis represents log transformation of the OD 600 value. Data are presented as mean ± standard deviations (SD) ( n = 2 independent experiments). b Genetic organization of the cdc cluster of P. prydzensis ACAM 620. c Heat map for the top ten most abundant proteins in the secretome of strain ACAM 620 grown on 0.5% (w/v) colloidal chitin as the sole carbon source. The colors in the heat map indicate relative protein abundance, ranging from high (red) to low abundance (blue). The data are presented as log transformation of the mean values of two biological replicates for each protein. These ten proteins account for 83.86% of the total protein abundance. The locus tag, protein annotation, type of signal peptides and CAZy family (glycosyl hydrolase (GH), carbohydrate-binding module (CBM) and auxiliary activity (AA)) are shown. Chitinolytic enzymes encoded by the cdc cluster are marked by solid circles and the other one by an empty circle. SpI, signal peptidase I cleavage site; SpII, signal peptidase II cleavage site. d Positive-mode Q-TOF-MS spectrum of products generated by the AA10 LPMO from strain ACAM 620 acting on 0.2% (w/v) squid pen β-chitin in the presence of 1 mM AscA. The insets show the negative control reactions without either LPMO or AscA, which did not generate detectable amounts of oxidized chitooligosaccharides. 100% relative intensity in the inserts represents 9.1 ×10 3 (control reaction without LPMO) and 7.0 ×10 3 (control reaction without AscA) arbitrary units (a.u.), respectively. Theoretical masses of relevant products are listed in Supplementary Table . DP, degree of polymerization; subscript OX, C1-oxidized chitooligosaccharides with a GlcNAc1A moiety; DP3 ox -Ac, DP3 ox lacking one acetyl group; DP4 ox -Ac, DP4 ox lacking one acetyl group. The graph shows a representative MS spectrum of at least three independent replicates. Source data are provided as a Source Data file.

Article Snippet: A total of 30 marine chitinolytic strains were used (Supplementary Tables and ), including 13 type strains of Pseudoalteromonas purchased from Deutsche Sammlung von Mikroorganismen and Zelkulturen (DSMZ) and Japan Collection of Microorganisms (JCM) and 17 non-type strains (6 Pseudoalteromonas strains and 11 Vibrio strains) isolated by our laboratory.

Techniques: Transformation Assay, Binding Assay, Activity Assay, Generated, Negative Control

Journal: Nature Communications

Article Title: A pathway for chitin oxidation in marine bacteria

doi: 10.1038/s41467-022-33566-5

Figure Lengend Snippet: a Distribution of the oxidative chitin utilization pathway in marine and terrestrial bacterial isolates. Except for terrestrial bacterium Achromobacter piechaudii ATCC 43553 belonging to Betaproteobacteria, all other bacterial strains with the complete oxidative chitin utilization pathway are from Gammaproteobacteria. For some representative strains containing more than one lpmo genes, only one lpmo gene was shown. b Comparison of the chitin-degrading abilities and related chitin-degrading genes of Pseudoalteromonas strains. Strains were cultivated in the minimal medium supplemented with 0.2% (w/v) shrimp shell α-chitin as the sole carbon source at 25 °C for 7 days. No bacterial growth was detectable for nine Pseudoalteromonas strains including P. aliena DSM 16473, P. aurantia DSM 6057, P. issachenkonii DSM 15925, P. lipolytica JCM 15903, P. luteoviolacea DSM 6061, P. rubra DSM 6842, P. tunicata DSM 14096, P. undina DSM 6065 and P . sp. SM9913, even though they were cultivated on α-chitin for 14 days at 25 °C. ND, undetectable growth; +, presence; -, absence. Growth data are presented as mean ± SD ( n = 3 independent experiments). Source data are provided as a Source Data file.

Article Snippet: A total of 30 marine chitinolytic strains were used (Supplementary Tables and ), including 13 type strains of Pseudoalteromonas purchased from Deutsche Sammlung von Mikroorganismen and Zelkulturen (DSMZ) and Japan Collection of Microorganisms (JCM) and 17 non-type strains (6 Pseudoalteromonas strains and 11 Vibrio strains) isolated by our laboratory.

Techniques: