cftr  (ATCC)


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    Structured Review

    ATCC cftr
    (a and b) Total and differential cell counts in BAL fluid from <t>Cftr</t> +/+ and Cftr −/− mice treated with PBS or B. cepacia (either BC7 or <t>ATCC</t> 25416). BAL fluid was harvested from each mouse by washing with three 1-ml aliquots of PBS via a cannulated trachea. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed with methanol, and stained with a modified Wright-Giemsa stain. Total numbers of cells and different cell types were determined by counting a minimum of 300 cells/slide. (a) Total numbers of cells; (b) differential cell counts. Bars, cell averages from two (ATCC 25416) and seven (PBS and BC7) animals in each group. SEM were calculated only for groups that had more than four animals. Asterisk, statistically significant difference between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (c) Surface expression of myeloid marker Ly-6G (Gr-1) on cells recovered in BAL fluid from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (d and e) Assessment of neutrophil activation in cells recovered by BAL. Shown is oxidant production, as indicated by surface expression of β 2 integrin CD11b (d) and oxidation of dihydrorhodamine (e) and by neutrophils recovered by BAL from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe).
    Cftr, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Enhanced Susceptibility to Pulmonary Infection with Burkholderia cepacia in Cftr−/− Mice"

    Article Title: Enhanced Susceptibility to Pulmonary Infection with Burkholderia cepacia in Cftr−/− Mice

    Journal: Infection and Immunity

    doi: 10.1128/IAI.69.8.5138-5150.2001

    (a and b) Total and differential cell counts in BAL fluid from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia (either BC7 or ATCC 25416). BAL fluid was harvested from each mouse by washing with three 1-ml aliquots of PBS via a cannulated trachea. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed with methanol, and stained with a modified Wright-Giemsa stain. Total numbers of cells and different cell types were determined by counting a minimum of 300 cells/slide. (a) Total numbers of cells; (b) differential cell counts. Bars, cell averages from two (ATCC 25416) and seven (PBS and BC7) animals in each group. SEM were calculated only for groups that had more than four animals. Asterisk, statistically significant difference between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (c) Surface expression of myeloid marker Ly-6G (Gr-1) on cells recovered in BAL fluid from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (d and e) Assessment of neutrophil activation in cells recovered by BAL. Shown is oxidant production, as indicated by surface expression of β 2 integrin CD11b (d) and oxidation of dihydrorhodamine (e) and by neutrophils recovered by BAL from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe).
    Figure Legend Snippet: (a and b) Total and differential cell counts in BAL fluid from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia (either BC7 or ATCC 25416). BAL fluid was harvested from each mouse by washing with three 1-ml aliquots of PBS via a cannulated trachea. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed with methanol, and stained with a modified Wright-Giemsa stain. Total numbers of cells and different cell types were determined by counting a minimum of 300 cells/slide. (a) Total numbers of cells; (b) differential cell counts. Bars, cell averages from two (ATCC 25416) and seven (PBS and BC7) animals in each group. SEM were calculated only for groups that had more than four animals. Asterisk, statistically significant difference between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (c) Surface expression of myeloid marker Ly-6G (Gr-1) on cells recovered in BAL fluid from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (d and e) Assessment of neutrophil activation in cells recovered by BAL. Shown is oxidant production, as indicated by surface expression of β 2 integrin CD11b (d) and oxidation of dihydrorhodamine (e) and by neutrophils recovered by BAL from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe).

    Techniques Used: Mouse Assay, Staining, Modification, Giemsa Stain, Expressing, Marker, Flow Cytometry, Cytometry, Activation Assay

    2) Product Images from "Quorum-Sensing System and Stationary-Phase Sigma Factor (rpoS) of the Onion Pathogen Burkholderia cepacia Genomovar I Type Strain, ATCC 25416"

    Article Title: Quorum-Sensing System and Stationary-Phase Sigma Factor (rpoS) of the Onion Pathogen Burkholderia cepacia Genomovar I Type Strain, ATCC 25416

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.69.3.1739-1747.2003

    (a) Map of the 5.5-kbp Sma I DNA fragment from B. cepacia ATCC 25416 isolated in this study. Shown are several enzyme restriction sites and the location of the rpoS gene within this fragment. Also shown is the position where a Km r -containing Bam HI fragment derived from pUC4K was cloned in the corresponding site of the rpoS gene to create pLCIKm. (b, c, and d) Effect of rpoS on stress responses of B. cepacia ATCC 25416. (b) Response to heat shock (50°C). Viability is expressed as a percentage of the number of CFU at time zero. (c) Response to osmotic shock (2 M NaCl). Viability is expressed as a percentage of the number of CFU at time zero. (d) Effect of rpoS mutation on oxidative stress. The sensitivity to H 2 O 2 was measured on cells grown for 16 h in LB at 30°C. The zones of inhibition were measured in millimeters. Filled symbols, WT strain; open symbols, B. cepacia 25416-RPOS strain.
    Figure Legend Snippet: (a) Map of the 5.5-kbp Sma I DNA fragment from B. cepacia ATCC 25416 isolated in this study. Shown are several enzyme restriction sites and the location of the rpoS gene within this fragment. Also shown is the position where a Km r -containing Bam HI fragment derived from pUC4K was cloned in the corresponding site of the rpoS gene to create pLCIKm. (b, c, and d) Effect of rpoS on stress responses of B. cepacia ATCC 25416. (b) Response to heat shock (50°C). Viability is expressed as a percentage of the number of CFU at time zero. (c) Response to osmotic shock (2 M NaCl). Viability is expressed as a percentage of the number of CFU at time zero. (d) Effect of rpoS mutation on oxidative stress. The sensitivity to H 2 O 2 was measured on cells grown for 16 h in LB at 30°C. The zones of inhibition were measured in millimeters. Filled symbols, WT strain; open symbols, B. cepacia 25416-RPOS strain.

    Techniques Used: Isolation, Derivative Assay, Clone Assay, Mutagenesis, Inhibition

    (a) Production of HSLs at different growth stages from B. cepacia ATCC 25416 (filled bars) and from B. cepacia ATCC 25416-RPOS (open bars). Values were determined as described in Materials and Methods. (b) rpoS promoter activity at different growth stages measured in B. cepacia ATCC 25416(pRPR2) (filled bars), B. cepacia 25416-I(pRPR2) (open bars), B. cepacia 25416-I(pRPR2) plus 100 nM C 8 -HSL (shaded bars), and B. cepacia ATCC 25416(pMP190) (striped bars). Values were determined as described in Materials and Methods.
    Figure Legend Snippet: (a) Production of HSLs at different growth stages from B. cepacia ATCC 25416 (filled bars) and from B. cepacia ATCC 25416-RPOS (open bars). Values were determined as described in Materials and Methods. (b) rpoS promoter activity at different growth stages measured in B. cepacia ATCC 25416(pRPR2) (filled bars), B. cepacia 25416-I(pRPR2) (open bars), B. cepacia 25416-I(pRPR2) plus 100 nM C 8 -HSL (shaded bars), and B. cepacia ATCC 25416(pMP190) (striped bars). Values were determined as described in Materials and Methods.

    Techniques Used: Activity Assay

    3) Product Images from "Enhanced Susceptibility to Pulmonary Infection with Burkholderia cepacia in Cftr−/− Mice"

    Article Title: Enhanced Susceptibility to Pulmonary Infection with Burkholderia cepacia in Cftr−/− Mice

    Journal: Infection and Immunity

    doi: 10.1128/IAI.69.8.5138-5150.2001

    Lung histology of Cftr +/+ mice after repeated exposure to PBS or B. cepacia isolate BC7. (a, c, and d) Hematoxylin- and eosin-stained sections; (b) PAS/Alcian blue-stained section. Representative sections of Cftr +/+ mice treated with PBS showing normal lung histology (a) with few PAS-positive goblet cells in the bronchiolar epithelia (b). Cftr +/+ mice exposed to BC7 demonstrated occasional perivascular and peribronchiolar inflammation (c) and small areas with mild hypertrophy of resident interstitial cells (d).
    Figure Legend Snippet: Lung histology of Cftr +/+ mice after repeated exposure to PBS or B. cepacia isolate BC7. (a, c, and d) Hematoxylin- and eosin-stained sections; (b) PAS/Alcian blue-stained section. Representative sections of Cftr +/+ mice treated with PBS showing normal lung histology (a) with few PAS-positive goblet cells in the bronchiolar epithelia (b). Cftr +/+ mice exposed to BC7 demonstrated occasional perivascular and peribronchiolar inflammation (c) and small areas with mild hypertrophy of resident interstitial cells (d).

    Techniques Used: Mouse Assay, Staining

    Immunolocalization of B. cepacia in the lungs of Cftr +/+ and Cftr −/− mice infected with isolate BC7. Paraffin sections (5 μm thick) were deparaffinized, rehydrated, heated in 10 mM sodium citrate buffer, pH 6.0, blocked with 5% normal donkey serum, and incubated overnight at 4°C with anti- B. cepacia antibody (diluted 1:1,000), and the bound antibody was detected by CY-3-conjugated anti-rabbit immunoglobulin G. (b and d) Bacteria in inflamed bronchoalveolar areas of Cftr −/− and Cftr +/+ mouse lungs, respectively; (f and h) bacteria in infiltrated and inflamed parenchyma of Cftr −/− and Cftr +/+ mouse lungs, respectively; (a, c, e, and g) hematoxylin- and eosin-stained sections corresponding to panels b, d, f, and h, respectively.
    Figure Legend Snippet: Immunolocalization of B. cepacia in the lungs of Cftr +/+ and Cftr −/− mice infected with isolate BC7. Paraffin sections (5 μm thick) were deparaffinized, rehydrated, heated in 10 mM sodium citrate buffer, pH 6.0, blocked with 5% normal donkey serum, and incubated overnight at 4°C with anti- B. cepacia antibody (diluted 1:1,000), and the bound antibody was detected by CY-3-conjugated anti-rabbit immunoglobulin G. (b and d) Bacteria in inflamed bronchoalveolar areas of Cftr −/− and Cftr +/+ mouse lungs, respectively; (f and h) bacteria in infiltrated and inflamed parenchyma of Cftr −/− and Cftr +/+ mouse lungs, respectively; (a, c, e, and g) hematoxylin- and eosin-stained sections corresponding to panels b, d, f, and h, respectively.

    Techniques Used: Mouse Assay, Infection, Incubation, Staining

    (a and b) Total and differential cell counts in BAL fluid from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia (either BC7 or ATCC 25416). BAL fluid was harvested from each mouse by washing with three 1-ml aliquots of PBS via a cannulated trachea. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed with methanol, and stained with a modified Wright-Giemsa stain. Total numbers of cells and different cell types were determined by counting a minimum of 300 cells/slide. (a) Total numbers of cells; (b) differential cell counts. Bars, cell averages from two (ATCC 25416) and seven (PBS and BC7) animals in each group. SEM were calculated only for groups that had more than four animals. Asterisk, statistically significant difference between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (c) Surface expression of myeloid marker Ly-6G (Gr-1) on cells recovered in BAL fluid from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (d and e) Assessment of neutrophil activation in cells recovered by BAL. Shown is oxidant production, as indicated by surface expression of β 2 integrin CD11b (d) and oxidation of dihydrorhodamine (e) and by neutrophils recovered by BAL from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe).
    Figure Legend Snippet: (a and b) Total and differential cell counts in BAL fluid from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia (either BC7 or ATCC 25416). BAL fluid was harvested from each mouse by washing with three 1-ml aliquots of PBS via a cannulated trachea. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed with methanol, and stained with a modified Wright-Giemsa stain. Total numbers of cells and different cell types were determined by counting a minimum of 300 cells/slide. (a) Total numbers of cells; (b) differential cell counts. Bars, cell averages from two (ATCC 25416) and seven (PBS and BC7) animals in each group. SEM were calculated only for groups that had more than four animals. Asterisk, statistically significant difference between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (c) Surface expression of myeloid marker Ly-6G (Gr-1) on cells recovered in BAL fluid from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (d and e) Assessment of neutrophil activation in cells recovered by BAL. Shown is oxidant production, as indicated by surface expression of β 2 integrin CD11b (d) and oxidation of dihydrorhodamine (e) and by neutrophils recovered by BAL from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe).

    Techniques Used: Mouse Assay, Staining, Modification, Giemsa Stain, Expressing, Marker, Flow Cytometry, Cytometry, Activation Assay

    4) Product Images from "Production of both l‐ and d‐ N‐acyl‐homoserine lactones by Burkholderia cepacia and Vibrio fischeri). Production of both l‐ and d‐ N‐acyl‐homoserine lactones by Burkholderia cepacia and Vibrio fischeri"

    Article Title: Production of both l‐ and d‐ N‐acyl‐homoserine lactones by Burkholderia cepacia and Vibrio fischeri). Production of both l‐ and d‐ N‐acyl‐homoserine lactones by Burkholderia cepacia and Vibrio fischeri

    Journal: MicrobiologyOpen

    doi: 10.1002/mbo3.1242

    (a) GC‐MS chromatogram of a standard solution of D and L hexanoyl (HHL), heptanoyl (HpHL, internal standard), octanoyl (OHL), decanoyl (DHL), 3‐oxobutanoyl (OBHL), and b . GC‐MS chromatogram showing the production of l ‐HHL, d ‐ and l ‐OHL, and possibly l ‐DHL from a 24‐h growth culture of Burkholderia cepacia . (See experimental for GC‐MS and extraction methods)
    Figure Legend Snippet: (a) GC‐MS chromatogram of a standard solution of D and L hexanoyl (HHL), heptanoyl (HpHL, internal standard), octanoyl (OHL), decanoyl (DHL), 3‐oxobutanoyl (OBHL), and b . GC‐MS chromatogram showing the production of l ‐HHL, d ‐ and l ‐OHL, and possibly l ‐DHL from a 24‐h growth culture of Burkholderia cepacia . (See experimental for GC‐MS and extraction methods)

    Techniques Used: Gas Chromatography-Mass Spectrometry

    Production of d,l ‐octanoyl‐homoserine lactone (OHL) with biomass (OD 600 ) curves during growth of B . cepacia . Data represent averages with standard deviations calculated from four replicates
    Figure Legend Snippet: Production of d,l ‐octanoyl‐homoserine lactone (OHL) with biomass (OD 600 ) curves during growth of B . cepacia . Data represent averages with standard deviations calculated from four replicates

    Techniques Used:

    Production of (a) l ‐octanoyl‐homoserine lactone ( l ‐OHL) and (b) d ‐octanoyl‐homoserine lactone ( d ‐OHL) with biomass (Abs OD 600) curves during growth of B . cepacia supplemented with 6.8 mmol of l ‐methionine ( l ‐Met), 6.8 mmol of d ‐methionine ( d ‐Met) or supplemented with 3.4 mmol of l ‐methionine and 3.4 mmol of d ‐methionine ( d,l ‐Met). Data represent averages with standard deviations calculated from four replicates
    Figure Legend Snippet: Production of (a) l ‐octanoyl‐homoserine lactone ( l ‐OHL) and (b) d ‐octanoyl‐homoserine lactone ( d ‐OHL) with biomass (Abs OD 600) curves during growth of B . cepacia supplemented with 6.8 mmol of l ‐methionine ( l ‐Met), 6.8 mmol of d ‐methionine ( d ‐Met) or supplemented with 3.4 mmol of l ‐methionine and 3.4 mmol of d ‐methionine ( d,l ‐Met). Data represent averages with standard deviations calculated from four replicates

    Techniques Used:

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  • cftr  (ATCC)
    97
    ATCC cftr
    (a and b) Total and differential cell counts in BAL fluid from <t>Cftr</t> +/+ and Cftr −/− mice treated with PBS or B. cepacia (either BC7 or <t>ATCC</t> 25416). BAL fluid was harvested from each mouse by washing with three 1-ml aliquots of PBS via a cannulated trachea. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed with methanol, and stained with a modified Wright-Giemsa stain. Total numbers of cells and different cell types were determined by counting a minimum of 300 cells/slide. (a) Total numbers of cells; (b) differential cell counts. Bars, cell averages from two (ATCC 25416) and seven (PBS and BC7) animals in each group. SEM were calculated only for groups that had more than four animals. Asterisk, statistically significant difference between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (c) Surface expression of myeloid marker Ly-6G (Gr-1) on cells recovered in BAL fluid from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (d and e) Assessment of neutrophil activation in cells recovered by BAL. Shown is oxidant production, as indicated by surface expression of β 2 integrin CD11b (d) and oxidation of dihydrorhodamine (e) and by neutrophils recovered by BAL from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe).
    Cftr, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cftr/product/ATCC
    Average 97 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    cftr - by Bioz Stars, 2022-10
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    (a and b) Total and differential cell counts in BAL fluid from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia (either BC7 or ATCC 25416). BAL fluid was harvested from each mouse by washing with three 1-ml aliquots of PBS via a cannulated trachea. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed with methanol, and stained with a modified Wright-Giemsa stain. Total numbers of cells and different cell types were determined by counting a minimum of 300 cells/slide. (a) Total numbers of cells; (b) differential cell counts. Bars, cell averages from two (ATCC 25416) and seven (PBS and BC7) animals in each group. SEM were calculated only for groups that had more than four animals. Asterisk, statistically significant difference between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (c) Surface expression of myeloid marker Ly-6G (Gr-1) on cells recovered in BAL fluid from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (d and e) Assessment of neutrophil activation in cells recovered by BAL. Shown is oxidant production, as indicated by surface expression of β 2 integrin CD11b (d) and oxidation of dihydrorhodamine (e) and by neutrophils recovered by BAL from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe).

    Journal: Infection and Immunity

    Article Title: Enhanced Susceptibility to Pulmonary Infection with Burkholderia cepacia in Cftr−/− Mice

    doi: 10.1128/IAI.69.8.5138-5150.2001

    Figure Lengend Snippet: (a and b) Total and differential cell counts in BAL fluid from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia (either BC7 or ATCC 25416). BAL fluid was harvested from each mouse by washing with three 1-ml aliquots of PBS via a cannulated trachea. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed with methanol, and stained with a modified Wright-Giemsa stain. Total numbers of cells and different cell types were determined by counting a minimum of 300 cells/slide. (a) Total numbers of cells; (b) differential cell counts. Bars, cell averages from two (ATCC 25416) and seven (PBS and BC7) animals in each group. SEM were calculated only for groups that had more than four animals. Asterisk, statistically significant difference between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (c) Surface expression of myeloid marker Ly-6G (Gr-1) on cells recovered in BAL fluid from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (d and e) Assessment of neutrophil activation in cells recovered by BAL. Shown is oxidant production, as indicated by surface expression of β 2 integrin CD11b (d) and oxidation of dihydrorhodamine (e) and by neutrophils recovered by BAL from Cftr +/+ and Cftr −/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe).

    Article Snippet: The lungs of both Cftr −/− and Cftr +/+ mice infected with ATCC 25416 demonstrated minimal evidence of pathology.

    Techniques: Mouse Assay, Staining, Modification, Giemsa Stain, Expressing, Marker, Flow Cytometry, Cytometry, Activation Assay

    Host cell responses of B. cepacia OMVs in A549 cells. OMVs were isolated from B. cepacia ATCC 25416 cultured in LB broth to late log phase. (a) Cytotoxicity in A549 cells treated with B. cepacia OMVs. Cells were treated with various concentrations of B. cepacia OMVs for 24 h, and cell viability was determined by a MTT assay. Data are presented as mean ± SD of three independent experiments. * P

    Journal: Virulence

    Article Title: Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses

    doi: 10.1080/21505594.2020.1802193

    Figure Lengend Snippet: Host cell responses of B. cepacia OMVs in A549 cells. OMVs were isolated from B. cepacia ATCC 25416 cultured in LB broth to late log phase. (a) Cytotoxicity in A549 cells treated with B. cepacia OMVs. Cells were treated with various concentrations of B. cepacia OMVs for 24 h, and cell viability was determined by a MTT assay. Data are presented as mean ± SD of three independent experiments. * P

    Article Snippet: These results indicate that B. cepacia ATCC 25416 produces OMVs during in vitro culture.

    Techniques: Isolation, Cell Culture, MTT Assay

    Inflammatory responsesin the lungs of mice administered B. cepacia OMVs. OMVs were isolated from culture supernatants of B. cepacia ATCC 25416 cultured in LB (OMVs/LB), LB with 0.5/9.5 μg/mL trimethoprim-sulfamethoxazole (OMVs/SXT), LB with 2 μg/mL meropenem (OMVs/MEM), or LB with 16 μg/mL ceftazidime (OMVs/CAZ). OMVs (20 μg of protein concentrations) were administered intratracheally, and mice were sacrificed 24 h after injection. (a) Histopathology of lungs. Lung tissues were stained by H E. PBS was administered as a control. Magnification, 100X. (b) Pro-inflammatory response to B. cepacia OMVs in the lungs of mice. Lung tissues were removed, and gene expression was assessed by qPCR. Data are presented as the mean ± SD of five mice. + P

    Journal: Virulence

    Article Title: Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses

    doi: 10.1080/21505594.2020.1802193

    Figure Lengend Snippet: Inflammatory responsesin the lungs of mice administered B. cepacia OMVs. OMVs were isolated from culture supernatants of B. cepacia ATCC 25416 cultured in LB (OMVs/LB), LB with 0.5/9.5 μg/mL trimethoprim-sulfamethoxazole (OMVs/SXT), LB with 2 μg/mL meropenem (OMVs/MEM), or LB with 16 μg/mL ceftazidime (OMVs/CAZ). OMVs (20 μg of protein concentrations) were administered intratracheally, and mice were sacrificed 24 h after injection. (a) Histopathology of lungs. Lung tissues were stained by H E. PBS was administered as a control. Magnification, 100X. (b) Pro-inflammatory response to B. cepacia OMVs in the lungs of mice. Lung tissues were removed, and gene expression was assessed by qPCR. Data are presented as the mean ± SD of five mice. + P

    Article Snippet: These results indicate that B. cepacia ATCC 25416 produces OMVs during in vitro culture.

    Techniques: Mouse Assay, Isolation, Cell Culture, Injection, Histopathology, Staining, Expressing, Real-time Polymerase Chain Reaction

    Cytotoxicity of A549 cells treated with OMVs from B. cepacia cultured with or without antibiotics. OMVs were isolated from culture supernatants of B. cepacia ATCC 25416 cultured in LB (OMVs/LB), LB with 0.5/9.5 μg/mL trimethoprim-sulfamethoxazole (OMVs/SXT), LB with 2 μg/mL meropenem (OMVs/MEM), or LB with 16 μg/mLceftazidime (OMVs/CAZ). Cells were treated with various concentrations of B. cepacia OMVs for 24 h, and cell viability was determined using the MTT assay. Data are presented as mean ± SD of three independent experiments. + P

    Journal: Virulence

    Article Title: Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses

    doi: 10.1080/21505594.2020.1802193

    Figure Lengend Snippet: Cytotoxicity of A549 cells treated with OMVs from B. cepacia cultured with or without antibiotics. OMVs were isolated from culture supernatants of B. cepacia ATCC 25416 cultured in LB (OMVs/LB), LB with 0.5/9.5 μg/mL trimethoprim-sulfamethoxazole (OMVs/SXT), LB with 2 μg/mL meropenem (OMVs/MEM), or LB with 16 μg/mLceftazidime (OMVs/CAZ). Cells were treated with various concentrations of B. cepacia OMVs for 24 h, and cell viability was determined using the MTT assay. Data are presented as mean ± SD of three independent experiments. + P

    Article Snippet: These results indicate that B. cepacia ATCC 25416 produces OMVs during in vitro culture.

    Techniques: Cell Culture, Isolation, MTT Assay

    Production of OMVs in B. cepacia ATCC 25416. Bacteria were cultured in LB broth to late log phase, and OMVs were isolated from the culture supernatants. (a) Transmission electron micrographs. (b) SDS-PAGE analysis of bacterial proteins. Lane 1, molecular weight marker; 2, bacterial lysates; 3, culture supernatant; 4, OMVs.

    Journal: Virulence

    Article Title: Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses

    doi: 10.1080/21505594.2020.1802193

    Figure Lengend Snippet: Production of OMVs in B. cepacia ATCC 25416. Bacteria were cultured in LB broth to late log phase, and OMVs were isolated from the culture supernatants. (a) Transmission electron micrographs. (b) SDS-PAGE analysis of bacterial proteins. Lane 1, molecular weight marker; 2, bacterial lysates; 3, culture supernatant; 4, OMVs.

    Article Snippet: These results indicate that B. cepacia ATCC 25416 produces OMVs during in vitro culture.

    Techniques: Cell Culture, Isolation, Transmission Assay, SDS Page, Molecular Weight, Marker

    Production of OMVs from B. cepacia ATCC 25416 cultured with subinhibitory concentrations of antibiotics. OMVs were isolated from culture supernatants of B. cepacia cultured in LB (OMVs/LB), LB with 2 μg/mL meropenem (OMVs/MEM), LB with 16 μg/mLceftazidime (OMVs/CAZ), or LB with 0.5/9.5 μg/mL trimethoprim-sulfamethoxazole (OMVs/SXT). (a) The size and number of OMV particles were determined using nanoparticle tracking analysis. The data are representative of three independent experiments with similar results. (b) The protein concentration of OMVs isolated from 1 L of bacterial culture was measured using a modified BCA assay. The data are presented as mean ± SD of three independent experiments. * P

    Journal: Virulence

    Article Title: Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses

    doi: 10.1080/21505594.2020.1802193

    Figure Lengend Snippet: Production of OMVs from B. cepacia ATCC 25416 cultured with subinhibitory concentrations of antibiotics. OMVs were isolated from culture supernatants of B. cepacia cultured in LB (OMVs/LB), LB with 2 μg/mL meropenem (OMVs/MEM), LB with 16 μg/mLceftazidime (OMVs/CAZ), or LB with 0.5/9.5 μg/mL trimethoprim-sulfamethoxazole (OMVs/SXT). (a) The size and number of OMV particles were determined using nanoparticle tracking analysis. The data are representative of three independent experiments with similar results. (b) The protein concentration of OMVs isolated from 1 L of bacterial culture was measured using a modified BCA assay. The data are presented as mean ± SD of three independent experiments. * P

    Article Snippet: These results indicate that B. cepacia ATCC 25416 produces OMVs during in vitro culture.

    Techniques: Cell Culture, Isolation, Protein Concentration, Modification, BIA-KA

    Expression of pro-inflammatory cytokine and chemokine genes in A549 cells treated with B. cepacia OMVs. OMVs were isolated from culture supernatants of B. cepacia ATCC 25416 cultured in LB (OMVs/LB), LB with 0.5/9.5 μg/mL trimethoprim-sulfamethoxazole (OMVs/SXT), LB with 2 μg/mL meropenem (OMVs/MEM), or LB with 16 μg/mL ceftazidime (OMVs/CAZ). Cells were treated with 5 μg/mL of OMVs for 6 h and gene expression was assessed by qPCR. Data are presented as mean ± SD of three independent experiments. + P

    Journal: Virulence

    Article Title: Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses

    doi: 10.1080/21505594.2020.1802193

    Figure Lengend Snippet: Expression of pro-inflammatory cytokine and chemokine genes in A549 cells treated with B. cepacia OMVs. OMVs were isolated from culture supernatants of B. cepacia ATCC 25416 cultured in LB (OMVs/LB), LB with 0.5/9.5 μg/mL trimethoprim-sulfamethoxazole (OMVs/SXT), LB with 2 μg/mL meropenem (OMVs/MEM), or LB with 16 μg/mL ceftazidime (OMVs/CAZ). Cells were treated with 5 μg/mL of OMVs for 6 h and gene expression was assessed by qPCR. Data are presented as mean ± SD of three independent experiments. + P

    Article Snippet: These results indicate that B. cepacia ATCC 25416 produces OMVs during in vitro culture.

    Techniques: Expressing, Isolation, Cell Culture, Real-time Polymerase Chain Reaction

    (A) Binding of BCM132 to 55 kDa TNFR1. Western blots of plasma membrane proteins was either incubated with 35S-labeled BCM132 or BC7. Bound bacteria were detected by autoradiography. (B) Stimulation of IL-8 response in IB3 cells by BCM132. IB3 cells were

    Journal: Cellular microbiology

    Article Title: Burkholderia cenocepacia ET12 Strain Activates TNFR1 Signaling in Cystic Fibrosis Airway Epithelial Cells

    doi: 10.1111/j.1462-5822.2007.01029.x

    Figure Lengend Snippet: (A) Binding of BCM132 to 55 kDa TNFR1. Western blots of plasma membrane proteins was either incubated with 35S-labeled BCM132 or BC7. Bound bacteria were detected by autoradiography. (B) Stimulation of IL-8 response in IB3 cells by BCM132. IB3 cells were

    Article Snippet: IB3 cells were incubated with TNF-α (3 ng/ml), BC7 or ATCC 25416 (each at an MOI of 10) for 5, 15 or 30 min.

    Techniques: Binding Assay, Western Blot, Incubation, Labeling, Autoradiography

    Effect of TNFR1 siRNA on transactivation of NF-κB by B. cenocepacia BC7. (A) Transactivation of NF-κB by bacteria: IB3 cells were co-transfected with NF-κB luciferase and renilla luciferase, and then incubated with BC7, ATCC 25146

    Journal: Cellular microbiology

    Article Title: Burkholderia cenocepacia ET12 Strain Activates TNFR1 Signaling in Cystic Fibrosis Airway Epithelial Cells

    doi: 10.1111/j.1462-5822.2007.01029.x

    Figure Lengend Snippet: Effect of TNFR1 siRNA on transactivation of NF-κB by B. cenocepacia BC7. (A) Transactivation of NF-κB by bacteria: IB3 cells were co-transfected with NF-κB luciferase and renilla luciferase, and then incubated with BC7, ATCC 25146

    Article Snippet: IB3 cells were incubated with TNF-α (3 ng/ml), BC7 or ATCC 25416 (each at an MOI of 10) for 5, 15 or 30 min.

    Techniques: Transfection, Luciferase, Incubation

    Binding of bacteria to TNFR1 in intact cells. (A) Expression of TNFR1: Non-permeabilized IB3 cells were incubated with monoclonal antibody to TNFR1 and bound antibody was detected with antimouse IgG conjugated with Alexafluor 488 and analyzed by FACS.

    Journal: Cellular microbiology

    Article Title: Burkholderia cenocepacia ET12 Strain Activates TNFR1 Signaling in Cystic Fibrosis Airway Epithelial Cells

    doi: 10.1111/j.1462-5822.2007.01029.x

    Figure Lengend Snippet: Binding of bacteria to TNFR1 in intact cells. (A) Expression of TNFR1: Non-permeabilized IB3 cells were incubated with monoclonal antibody to TNFR1 and bound antibody was detected with antimouse IgG conjugated with Alexafluor 488 and analyzed by FACS.

    Article Snippet: IB3 cells were incubated with TNF-α (3 ng/ml), BC7 or ATCC 25416 (each at an MOI of 10) for 5, 15 or 30 min.

    Techniques: Binding Assay, Expressing, Incubation, FACS

    Activation of TNFR1-related signaling pathway by B. cenocepacia . (A) Western blot analysis of immunoprecipitates: IB3 cells were incubated with either TNF-α (3ng/ml) or B. cenocepacia , BC7 or ATCC 25416 at MOI of 10 for 5, 15 or 30 min and lysed.

    Journal: Cellular microbiology

    Article Title: Burkholderia cenocepacia ET12 Strain Activates TNFR1 Signaling in Cystic Fibrosis Airway Epithelial Cells

    doi: 10.1111/j.1462-5822.2007.01029.x

    Figure Lengend Snippet: Activation of TNFR1-related signaling pathway by B. cenocepacia . (A) Western blot analysis of immunoprecipitates: IB3 cells were incubated with either TNF-α (3ng/ml) or B. cenocepacia , BC7 or ATCC 25416 at MOI of 10 for 5, 15 or 30 min and lysed.

    Article Snippet: IB3 cells were incubated with TNF-α (3 ng/ml), BC7 or ATCC 25416 (each at an MOI of 10) for 5, 15 or 30 min.

    Techniques: Activation Assay, Western Blot, Incubation

    Effect of TNF-α neutralizing antibody on B. cenocepacia -stimulated IL-8 response in IB3 cells (A) IL-8 response: IB3 cells were incubated with B. cenocepacia BC7 or ATCC 25416 for 1, 3, 6 or 24 h, and IL-8 in the cell culture media was quantified

    Journal: Cellular microbiology

    Article Title: Burkholderia cenocepacia ET12 Strain Activates TNFR1 Signaling in Cystic Fibrosis Airway Epithelial Cells

    doi: 10.1111/j.1462-5822.2007.01029.x

    Figure Lengend Snippet: Effect of TNF-α neutralizing antibody on B. cenocepacia -stimulated IL-8 response in IB3 cells (A) IL-8 response: IB3 cells were incubated with B. cenocepacia BC7 or ATCC 25416 for 1, 3, 6 or 24 h, and IL-8 in the cell culture media was quantified

    Article Snippet: IB3 cells were incubated with TNF-α (3 ng/ml), BC7 or ATCC 25416 (each at an MOI of 10) for 5, 15 or 30 min.

    Techniques: Incubation, Cell Culture