Journal: International Journal of Molecular Sciences

Article Title: A Genetically-Engineered Thyroid Gland Built for Selective Triiodothyronine Secretion

doi: 10.3390/ijms26157166

Figure Lengend Snippet: Expression of markers of T 3 action in the liver of ChEL-KI mice. ( A ) Expression of malic enzyme 1 (ME1). Lower panel: SDS-polyacrylamide gel electrophoresis of liver homogenates from 3-month-old ChEL-KI mice, cog / cog animals, and wild-type (WT) mice, followed by immunoblotting with anti-ME1 antibodies and anti-β-actin antibodies as indicated. Upper panel: Graph shows the densitometric quantitation of hepatic ME1 protein expression normalized to β-actin. ( B ) Expression of type-1 deiodinase (D1). Lower panel: SDS-polyacrylamide gel electrophoresis of liver homogenates from ChEL-KI mice, cog / cog animals, and WT controls, followed by immunoblotting with anti-D1 antibodies and anti-β-actin antibodies as indicated. Upper panel: Graph shows the densitometric quantitation of hepatic D1 protein expression normalized to β-actin. Each dot represents an individual animal, with circles representing females and squares representing males; n = 8–11 animals per group; mean ± SD; ns, not significant ( p > 0.05), p ; **** p < 0.0001 (one-way ANOVA with Tukey’s post hoc test).

Article Snippet: DYKDDDDK Tag rabbit mAb (D6W5B, Cell Signaling Technology, Danvers, MA, USA), rabbit monoclonal [EPR9730] to thyroglobulin Ab (Abcam, Cambridge, UK), T 3 mouse mAb (3A6, Invitrogen, Waltham, MA, USA), DIO1 (B-7) mAb (sc-515198, Santa Cruz Biotechnologies, Dallas, TX, USA), ME1 (C-6) mAb (sc-365891, Santa Cruz Biotechnologies, Dallas, TX, USA), Ki67 mAb (ab16667, Abcam, Cambridge, UK), KDEL (10C) mAb (ADI-SPA-827-D Enzo Life Sciences, Long Island, NY, USA), anti-Flag M2 mAb (F1804, MilliporeSigma, Burlington, MA, USA), β-Actin (C4) mAb (sc-47778, Santa Cruz Biotechnologies, Dallas, TX, USA), and HSP 90α/β (F-8) mAb (sc-13119, Santa Cruz Biotechnologies, Dallas, TX, USA) were used.

Techniques: Expressing, Polyacrylamide Gel Electrophoresis, Western Blot, Quantitation Assay

Journal: Advanced Science

Article Title: Coactosin‐Like Protein Reduces Prostaglandin D 2 Production in Alveolar Macrophages and Alleviates Allergic Airway Inflammation

doi: 10.1002/advs.202501673

Figure Lengend Snippet: Effect of reconstitution of clodronate‐treated mice with littermate control or Cotl1 −/‐ alveolar macrophages on HDM‐induced pulmonary immune cell accumulation. A) WT mice received clodronate liposomes to deplete endogenous AMs. After 72 h, the mice were intranasally injected with WT or Cotl1 −/‐ AMs and treated with HDM. On day 23, mice were sacrificed, and lung tissue single‐cell suspensions were prepared and analyzed by flow cytometry. B) Frequencies of IRF5 + M1 macrophages, CD206 + M2 macrophages, and IL‐10 + M2‐like macrophages among the CD45 + CD11c + CD11b − F4/80 + population ( n = 6). C) Frequencies of IL‐4 + Th2 cells among the CD4 + population, CD49b + basophils among the IgE + population, Siglec‐F + eosinophils among the CD45 + CD11c + population, Sca‐1 + KLRG1 + ILC2 cells among the CD45 + CD25 + Lin − population, and CD117 + mast cells among the IgE + population ( n = 6). D) Frequencies of CRTH2 + cells among the macrophage population ( n = 6). E) Frequencies of CRTH2 + cells among the basophil population ( n = 6). F) Frequencies of CRTH2 + cells among the eosinophil population ( n = 6). G) Frequencies of CRTH2 + cells among the ILC2 population ( n = 6). H) Frequencies of CRTH2 + cells among the mast cell population ( n = 6). I) Frequencies of CRTH2 + cells among the Th2 cell population ( n = 6). Data shown in (B), (C), and (E)‐(I) were presented as median ± interquartile range. p values were assessed using Tukey's multiple comparisons test following one‐way ANOVA for (B), (C), and (E–I). p < 0.05 was considered statistically significant.

Article Snippet: Antibodies for CLP (10781‐1‐AP), HPGDS (22522‐1‐AP), mPGES‐2 (10881‐1‐AP), CRTH2 (25264‐1‐AP), and β‐actin (60008‐1‐lg) were purchased from Proteintech (Hubei, China).

Techniques: Control, Liposomes, Injection, Flow Cytometry

Journal: Advanced Science

Article Title: Coactosin‐Like Protein Reduces Prostaglandin D 2 Production in Alveolar Macrophages and Alleviates Allergic Airway Inflammation

doi: 10.1002/advs.202501673

Figure Lengend Snippet: AMs from Cotl1 −/− mice confer exacerbated airway inflammation via CRTH2. A) WT mice received clodronate liposomes to deplete endogenous AMs. After 72 h, the mice were intranasally injected with Cotl1 −/‐ AMs. Mice were challenged with HDM and treated with OC000459 . On day 23, mice were euthanized, and lung tissue was analyzed. B) The levels of Th2 cytokines (IL‐4, IL‐5, and IL‐13) in lung tissue extracts were determined by ELISA ( n = 6). C,D) Representative lung samples stained with (C) H&E and (D) PAS ( n = 6). Scale bar: 50 µm. E) Frequencies of IL‐4 + Th2 cells among the CD4 + population, CD49b + basophils among the IgE + population, Siglec‐F + eosinophils among the CD45 + CD11c + population, Sca‐1 + KLRG1 + ILC2 cells among the CD45 + CD25 + Lin − population, and CD117 + mast cells among the IgE + population ( n = 6). Data shown in (B–D) were presented as mean ± SD, and data shown in (E) were presented as median ± interquartile range. p values were assessed using Tukey's multiple comparisons test following two‐way ANOVA for (B), and Tukey's multiple comparisons test following one‐way ANOVA for (C–E). p < 0.05 was considered statistically significant.

Article Snippet: Antibodies for CLP (10781‐1‐AP), HPGDS (22522‐1‐AP), mPGES‐2 (10881‐1‐AP), CRTH2 (25264‐1‐AP), and β‐actin (60008‐1‐lg) were purchased from Proteintech (Hubei, China).

Techniques: Liposomes, Injection, Enzyme-linked Immunosorbent Assay, Staining