mgcl2  (Solis BioDyne)


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  • 95
    Name:
    25 mM MgCl2
    Description:
    25 mM MgCl2 is used for adjusting Mg2 concentration in PCR assay
    Catalog Number:
    05-11-00025
    Price:
    None
    Category:
    Buffers
    Applications:
    https://www.solisbiodyne.com/pics/thumb_8304_4_25_mM_MgCl2_25ml.jpg
    Size:
    2 5 ml
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    Structured Review

    Solis BioDyne mgcl2
    25 mM MgCl2 is used for adjusting Mg2 concentration in PCR assay
    https://www.bioz.com/result/mgcl2/product/Solis BioDyne
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mgcl2 - by Bioz Stars, 2021-09
    95/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Highly efficient genome modification of cultured primordial germ cells with lentiviral vectors to generate transgenic songbirds
    Article Snippet: .. For each PCR 50 to 100 ng gDNA was used in a 20 µl reaction mixture that contained 12.5 µl H2O, 2 µl primer mix (10 µM), 0.4 µl dNTPs (10 µM), as well as 2 µl buffer B1 (10X), 1.6 µl MgCl2 (25 mM) and 0.5 µl FIREPol® DNA polymerase (all three from Solis BioDyne). ..

    Article Title: Altered DNA methylation profiles in blood from patients with sporadic Creutzfeldt–Jakob disease
    Article Snippet: .. PCR amplification of bisulfite-converted DNA was performed in a Veriti 96-Well Thermo Cycler (Thermofisher, MA, USA) using the following mastermix per 1 μl bisulifte-converted DNA: 1X Buffer B1 (Solis BioDyne, EE), 25 mM MgCl2 (Solis BioDyne, EE), 10 mM dNTP mix (Promega, WI, USA), 10 μM forward/reverse primer mix (Supplementary Table 5, online resource), 1 U HotFire Polymase (Solis BioDyne, EE) and RNAse-free water (Thermofisher, MA, USA) to a total reaction volume of 20 μl. ..

    Article Title: Highly Efficient Genome Modification of Cultured Primordial Germ Cells with Lentiviral Vectors to Generate Transgenic Songbirds
    Article Snippet: .. For each PCR 50–100 ng gDNA was used in a 20 μL reaction mixture that contained 12.5 μL H2 O, 2 μL primer mix (10 μM), 0.4 μL dNTPs (10 μM), as well as 2 μL buffer B1 (10×), 1.6 μL MgCl2 (25 mM), and 0.5 μL FIREPol DNA polymerase (all three from Solis BioDyne). ..

    Article Title: Polymorphism of insulin-like growth factor 1 gene and its relationship with reproductive performances and milk yield in Sarda dairy sheep
    Article Snippet: .. The PCR analysis for exon 3 was carried out in 25 μl total volume containing 2.5 μL of 10X PCR Reaction Buffer B (Mg2+ free) [0.8 M Tris HCl (pH 8.4), 0.2 M (NH4 )2 SO4, 0.2% w/v Tween-20]; 2.5 μl of 25 Mm MgCl2, 4 μl of 0.2 mM dNTPs; 1 μl of 0.1 mM of each forward and reverse primers, and 2 U of FIREPol DNA Polymerase (5 U/μl) (Solis BioDyne, Tartu, Estonia). ..

    Article Title: Effects of Genetic Polymorphisms of Drug Transporter ABCB1 (MDR1) and Cytochrome P450 Enzymes CYP2A6, CYP2B6 on Nicotine Addiction and Smoking Cessation
    Article Snippet: .. A total volume of 25 μl containing 200 μM of each dATP, dCTP, dGTP, dTTP, 2.5 mM MgCl2, BSA, and 12.5 pmol of each primer, 1 unit of Taq DNA Polymerase, and 100 ng of genomic DNA used as PCR mixture (Solis BioDyne, Tartu, Estonia). ..

    Amplification:

    Article Title: Altered DNA methylation profiles in blood from patients with sporadic Creutzfeldt–Jakob disease
    Article Snippet: .. PCR amplification of bisulfite-converted DNA was performed in a Veriti 96-Well Thermo Cycler (Thermofisher, MA, USA) using the following mastermix per 1 μl bisulifte-converted DNA: 1X Buffer B1 (Solis BioDyne, EE), 25 mM MgCl2 (Solis BioDyne, EE), 10 mM dNTP mix (Promega, WI, USA), 10 μM forward/reverse primer mix (Supplementary Table 5, online resource), 1 U HotFire Polymase (Solis BioDyne, EE) and RNAse-free water (Thermofisher, MA, USA) to a total reaction volume of 20 μl. ..

    Article Title: A cell-of-origin epigenetic tracer reveals clinically distinct subtypes of high-grade serous ovarian cancer
    Article Snippet: .. PCRs were set-up in 30-μl reactions using 3 μl of 10x Hot Fire Pol Buffer (Solis BioDyne), 4 μl of 10 mM dNTPs (Fisher Scientific), 2.25 μl of 25 mM MgCl2 (Solis BioDyne), 0.6 μl of amplicon-specific forward and reverse primer (10 μM each), 0.3 μl of Hot FirePol DNA Polymerase (5 U/μl; Solis Biodyne), 1 μl of bisulfite-converted DNA, and 18.25 μl of double distilled water. ..

    Article Title: Molecular assessment of kelch13 non-synonymous mutations in Plasmodium falciparum isolates from Central African Republic (2017–2019)
    Article Snippet: .. Five μl DNA was amplified with 0.25 μM each primer, 0.2 mM dNTP, 2.5 mM MgCl2 , and 1.25 U Taq DNA polymerase (Solis Biodyne, Estonia), in 25 µl volume using the following cycling program: 15 min at 95 °C, then 35 cycles of 30 s at 95 °C, 2 min at 58 °C, 2 min at 72 °C, and final extension 10 min at 72 °C. ..

    Article Title: Genetic diversity of Helosciadium repens (Jacq.) W.D.J. Koch (Apiaceae) in Germany, a Crop Wild Relative of celery. Genetic diversity of Helosciadium repens (Jacq.) W.D.J. Koch (Apiaceae) in Germany, a Crop Wild Relative of celery
    Article Snippet: .. Microsatellite amplification was carried out for all 49 primer sets in a final volume of 20 µl, containing 1 µl DMSO, 2 µl 10× reaction buffer B (Solis BioDyne), 1.6 µl MgCl2 (25 mM), 0.4 dNTP mix (10 mM), 0.6 µl of each primer (0.3 µM), 1 µl DNA, and 0.1 µl FIREPol Taq‐polymerase (Solis BioDyne). ..

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  • 86
    Solis BioDyne mgcl2
    Mgcl2, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgcl2/product/Solis BioDyne
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mgcl2 - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

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