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SP4 binds the promoter of <t>PHF14</t> to mediate its transcription. A, Bioinformatics analysis (UCSC Genome Browser) predicted the SP4 binding site in the promoter of PHF14. B, Correlation analysis between SP4 and PHF14 using TCGA data. C, mRNA correlation between SP4 and PHF14 in ESCC tissues. D, ChIP-qRT-PCR analysis showed that SP4 binds the promoter of PHF14. E, ESCC cells were transfected with pGL3-PHF14-luc (target sequences of SP4), and the luciferase activity was examined at 48 h after transfection. Renilla luciferase was known as the internal control. F, ESCC cells were co-transfected with pGL3-PHF14-luc and SP4 siRNAs; the luciferase activity was determined. Renilla luciferase was known as the internal control. G, ESCC cells were cotransfected with pGL3-PHF14-luc and SP4 siRNAs; the luciferase activity was determined. Renilla luciferase was known as the internal control. H, PHF14 mRNA expression after transfection with SP4 siRNA in ESCC cells. GAPDH was used as an internal reference. I, PHF14 mRNA expression after transfection with SP4 overexpression plasmid in ESCC cells. GAPDH was used as an internal reference. J, SP4 and PHF14 expressions were measured after transfection with SP4 siRNA in ESCC cells. GAPDH was used as an internal reference. K, SP4 and PHF14 expressions were measured after transfection with SP4 overexpression plasmid in ESCC cells. GAPDH was used as an internal reference. L, SP4 and PHF14 expressions were measured after SP4 knockdown in ESCC cells. GAPDH was used as an internal reference. Each experiment was performed in triplicate. According to the data characteristics, quantitative data of B and C were analyzed by two-way ANOVA, quantitative data of D – I were analyzed by Student t test. *, P < 0.05.
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SP4 binds the promoter of <t>PHF14</t> to mediate its transcription. A, Bioinformatics analysis (UCSC Genome Browser) predicted the SP4 binding site in the promoter of PHF14. B, Correlation analysis between SP4 and PHF14 using TCGA data. C, mRNA correlation between SP4 and PHF14 in ESCC tissues. D, ChIP-qRT-PCR analysis showed that SP4 binds the promoter of PHF14. E, ESCC cells were transfected with pGL3-PHF14-luc (target sequences of SP4), and the luciferase activity was examined at 48 h after transfection. Renilla luciferase was known as the internal control. F, ESCC cells were co-transfected with pGL3-PHF14-luc and SP4 siRNAs; the luciferase activity was determined. Renilla luciferase was known as the internal control. G, ESCC cells were cotransfected with pGL3-PHF14-luc and SP4 siRNAs; the luciferase activity was determined. Renilla luciferase was known as the internal control. H, PHF14 mRNA expression after transfection with SP4 siRNA in ESCC cells. GAPDH was used as an internal reference. I, PHF14 mRNA expression after transfection with SP4 overexpression plasmid in ESCC cells. GAPDH was used as an internal reference. J, SP4 and PHF14 expressions were measured after transfection with SP4 siRNA in ESCC cells. GAPDH was used as an internal reference. K, SP4 and PHF14 expressions were measured after transfection with SP4 overexpression plasmid in ESCC cells. GAPDH was used as an internal reference. L, SP4 and PHF14 expressions were measured after SP4 knockdown in ESCC cells. GAPDH was used as an internal reference. Each experiment was performed in triplicate. According to the data characteristics, quantitative data of B and C were analyzed by two-way ANOVA, quantitative data of D – I were analyzed by Student t test. *, P < 0.05.
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SP4 binds the promoter of <t>PHF14</t> to mediate its transcription. A, Bioinformatics analysis (UCSC Genome Browser) predicted the SP4 binding site in the promoter of PHF14. B, Correlation analysis between SP4 and PHF14 using TCGA data. C, mRNA correlation between SP4 and PHF14 in ESCC tissues. D, ChIP-qRT-PCR analysis showed that SP4 binds the promoter of PHF14. E, ESCC cells were transfected with pGL3-PHF14-luc (target sequences of SP4), and the luciferase activity was examined at 48 h after transfection. Renilla luciferase was known as the internal control. F, ESCC cells were co-transfected with pGL3-PHF14-luc and SP4 siRNAs; the luciferase activity was determined. Renilla luciferase was known as the internal control. G, ESCC cells were cotransfected with pGL3-PHF14-luc and SP4 siRNAs; the luciferase activity was determined. Renilla luciferase was known as the internal control. H, PHF14 mRNA expression after transfection with SP4 siRNA in ESCC cells. GAPDH was used as an internal reference. I, PHF14 mRNA expression after transfection with SP4 overexpression plasmid in ESCC cells. GAPDH was used as an internal reference. J, SP4 and PHF14 expressions were measured after transfection with SP4 siRNA in ESCC cells. GAPDH was used as an internal reference. K, SP4 and PHF14 expressions were measured after transfection with SP4 overexpression plasmid in ESCC cells. GAPDH was used as an internal reference. L, SP4 and PHF14 expressions were measured after SP4 knockdown in ESCC cells. GAPDH was used as an internal reference. Each experiment was performed in triplicate. According to the data characteristics, quantitative data of B and C were analyzed by two-way ANOVA, quantitative data of D – I were analyzed by Student t test. *, P < 0.05.
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SP4 binds the promoter of <t>PHF14</t> to mediate its transcription. A, Bioinformatics analysis (UCSC Genome Browser) predicted the SP4 binding site in the promoter of PHF14. B, Correlation analysis between SP4 and PHF14 using TCGA data. C, mRNA correlation between SP4 and PHF14 in ESCC tissues. D, ChIP-qRT-PCR analysis showed that SP4 binds the promoter of PHF14. E, ESCC cells were transfected with pGL3-PHF14-luc (target sequences of SP4), and the luciferase activity was examined at 48 h after transfection. Renilla luciferase was known as the internal control. F, ESCC cells were co-transfected with pGL3-PHF14-luc and SP4 siRNAs; the luciferase activity was determined. Renilla luciferase was known as the internal control. G, ESCC cells were cotransfected with pGL3-PHF14-luc and SP4 siRNAs; the luciferase activity was determined. Renilla luciferase was known as the internal control. H, PHF14 mRNA expression after transfection with SP4 siRNA in ESCC cells. GAPDH was used as an internal reference. I, PHF14 mRNA expression after transfection with SP4 overexpression plasmid in ESCC cells. GAPDH was used as an internal reference. J, SP4 and PHF14 expressions were measured after transfection with SP4 siRNA in ESCC cells. GAPDH was used as an internal reference. K, SP4 and PHF14 expressions were measured after transfection with SP4 overexpression plasmid in ESCC cells. GAPDH was used as an internal reference. L, SP4 and PHF14 expressions were measured after SP4 knockdown in ESCC cells. GAPDH was used as an internal reference. Each experiment was performed in triplicate. According to the data characteristics, quantitative data of B and C were analyzed by two-way ANOVA, quantitative data of D – I were analyzed by Student t test. *, P < 0.05.
Anti Phf14, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti phf14/product/Proteintech
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anti phf14 - by Bioz Stars, 2025-12
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SP4 binds the promoter of PHF14 to mediate its transcription. A, Bioinformatics analysis (UCSC Genome Browser) predicted the SP4 binding site in the promoter of PHF14. B, Correlation analysis between SP4 and PHF14 using TCGA data. C, mRNA correlation between SP4 and PHF14 in ESCC tissues. D, ChIP-qRT-PCR analysis showed that SP4 binds the promoter of PHF14. E, ESCC cells were transfected with pGL3-PHF14-luc (target sequences of SP4), and the luciferase activity was examined at 48 h after transfection. Renilla luciferase was known as the internal control. F, ESCC cells were co-transfected with pGL3-PHF14-luc and SP4 siRNAs; the luciferase activity was determined. Renilla luciferase was known as the internal control. G, ESCC cells were cotransfected with pGL3-PHF14-luc and SP4 siRNAs; the luciferase activity was determined. Renilla luciferase was known as the internal control. H, PHF14 mRNA expression after transfection with SP4 siRNA in ESCC cells. GAPDH was used as an internal reference. I, PHF14 mRNA expression after transfection with SP4 overexpression plasmid in ESCC cells. GAPDH was used as an internal reference. J, SP4 and PHF14 expressions were measured after transfection with SP4 siRNA in ESCC cells. GAPDH was used as an internal reference. K, SP4 and PHF14 expressions were measured after transfection with SP4 overexpression plasmid in ESCC cells. GAPDH was used as an internal reference. L, SP4 and PHF14 expressions were measured after SP4 knockdown in ESCC cells. GAPDH was used as an internal reference. Each experiment was performed in triplicate. According to the data characteristics, quantitative data of B and C were analyzed by two-way ANOVA, quantitative data of D – I were analyzed by Student t test. *, P < 0.05.

Journal: Molecular Cancer Research

Article Title: SP4 Facilitates Esophageal Squamous Cell Carcinoma Progression by Activating PHF14 Transcription and Wnt/Β-Catenin Signaling

doi: 10.1158/1541-7786.MCR-22-0835

Figure Lengend Snippet: SP4 binds the promoter of PHF14 to mediate its transcription. A, Bioinformatics analysis (UCSC Genome Browser) predicted the SP4 binding site in the promoter of PHF14. B, Correlation analysis between SP4 and PHF14 using TCGA data. C, mRNA correlation between SP4 and PHF14 in ESCC tissues. D, ChIP-qRT-PCR analysis showed that SP4 binds the promoter of PHF14. E, ESCC cells were transfected with pGL3-PHF14-luc (target sequences of SP4), and the luciferase activity was examined at 48 h after transfection. Renilla luciferase was known as the internal control. F, ESCC cells were co-transfected with pGL3-PHF14-luc and SP4 siRNAs; the luciferase activity was determined. Renilla luciferase was known as the internal control. G, ESCC cells were cotransfected with pGL3-PHF14-luc and SP4 siRNAs; the luciferase activity was determined. Renilla luciferase was known as the internal control. H, PHF14 mRNA expression after transfection with SP4 siRNA in ESCC cells. GAPDH was used as an internal reference. I, PHF14 mRNA expression after transfection with SP4 overexpression plasmid in ESCC cells. GAPDH was used as an internal reference. J, SP4 and PHF14 expressions were measured after transfection with SP4 siRNA in ESCC cells. GAPDH was used as an internal reference. K, SP4 and PHF14 expressions were measured after transfection with SP4 overexpression plasmid in ESCC cells. GAPDH was used as an internal reference. L, SP4 and PHF14 expressions were measured after SP4 knockdown in ESCC cells. GAPDH was used as an internal reference. Each experiment was performed in triplicate. According to the data characteristics, quantitative data of B and C were analyzed by two-way ANOVA, quantitative data of D – I were analyzed by Student t test. *, P < 0.05.

Article Snippet: The GAPDH (60004-1-Ig), β-catenin (51067-2-AP), PHF14 (ProteinTech, catalog no. 24787–1-AP, RRID: AB_2879724), and mouse IgG (ProteinTech, catalog no. B900620, RRID: AB_2883054) antibodies were purchased from ProteinTech Group.

Techniques: Binding Assay, Quantitative RT-PCR, Transfection, Luciferase, Activity Assay, Expressing, Over Expression, Plasmid Preparation

PHF14 promotes ESCC cell proliferation and inhibits apoptosis in vitro . A, Viability of ESCC cells after transfection with PHF14 siRNA. B, Viability of ESCC cells after transfection with PHF14 overexpression plasmid. C, Cell number of ESCC cells after transfection with PHF14 siRNA. D, Cell number of ESCC cells after transfection with PHF14 overexpression plasmid. E, The cell cycle of ESCC cells after transfection with PHF14 siRNA was analyzed by flow cytometry. F, The cell cycle of ESCC cells after transfection with PHF14 overexpression plasmid was analyzed by flow cytometry. G, Apoptosis of ESCC cells after transfection with PHF14 siRNA was examined by flow cytometry. H, Apoptosis of ESCC cells after transfection with PHF14 overexpression plasmid was examined by flow cytometry. I, PHF14 mRNA expression after transfection with PHF14 siRNA in ESCC cells. J, PHF14 mRNA expression after transfection with PHF14 overexpression plasmid in ESCC cells. K, PHF14, Wnt3a, β-catenin, CDK4, CDK6, Cyclin D1, and active caspase 3 expressions were measured after transfection with PHF14 siRNA in ESCC cells. GAPDH was used as an internal reference. L, PHF14, Wnt3a, β-catenin, CDK4, CDK6, Cyclin D1, and active caspase 3 expressions were measured after transfection with PHF14 overexpression plasmid in ESCC cells. GAPDH was used as an internal reference. Each experiment was performed in triplicate. According to the data characteristics, quantitative data of E – J were analyzed by Student t test, quantitative data of A – D were analyzed by Pearson χ 2 test. *, P < 0.05.

Journal: Molecular Cancer Research

Article Title: SP4 Facilitates Esophageal Squamous Cell Carcinoma Progression by Activating PHF14 Transcription and Wnt/Β-Catenin Signaling

doi: 10.1158/1541-7786.MCR-22-0835

Figure Lengend Snippet: PHF14 promotes ESCC cell proliferation and inhibits apoptosis in vitro . A, Viability of ESCC cells after transfection with PHF14 siRNA. B, Viability of ESCC cells after transfection with PHF14 overexpression plasmid. C, Cell number of ESCC cells after transfection with PHF14 siRNA. D, Cell number of ESCC cells after transfection with PHF14 overexpression plasmid. E, The cell cycle of ESCC cells after transfection with PHF14 siRNA was analyzed by flow cytometry. F, The cell cycle of ESCC cells after transfection with PHF14 overexpression plasmid was analyzed by flow cytometry. G, Apoptosis of ESCC cells after transfection with PHF14 siRNA was examined by flow cytometry. H, Apoptosis of ESCC cells after transfection with PHF14 overexpression plasmid was examined by flow cytometry. I, PHF14 mRNA expression after transfection with PHF14 siRNA in ESCC cells. J, PHF14 mRNA expression after transfection with PHF14 overexpression plasmid in ESCC cells. K, PHF14, Wnt3a, β-catenin, CDK4, CDK6, Cyclin D1, and active caspase 3 expressions were measured after transfection with PHF14 siRNA in ESCC cells. GAPDH was used as an internal reference. L, PHF14, Wnt3a, β-catenin, CDK4, CDK6, Cyclin D1, and active caspase 3 expressions were measured after transfection with PHF14 overexpression plasmid in ESCC cells. GAPDH was used as an internal reference. Each experiment was performed in triplicate. According to the data characteristics, quantitative data of E – J were analyzed by Student t test, quantitative data of A – D were analyzed by Pearson χ 2 test. *, P < 0.05.

Article Snippet: The GAPDH (60004-1-Ig), β-catenin (51067-2-AP), PHF14 (ProteinTech, catalog no. 24787–1-AP, RRID: AB_2879724), and mouse IgG (ProteinTech, catalog no. B900620, RRID: AB_2883054) antibodies were purchased from ProteinTech Group.

Techniques: In Vitro, Transfection, Over Expression, Plasmid Preparation, Flow Cytometry, Expressing

SP4 facilitates ESCC progression via modulating the Wnt/β-catenin signaling pathway by promoting PHF14 transcription. A, Viability of ESCC cells after cotransfection with SP4 siRNA-1 and PHF14 overexpression plasmid. B, Cell number of ESCC cells after cotransfection with SP4 siRNA-1 and PHF14 overexpression plasmid. C, The cell cycle of ESCC cells after cotransfection with SP4 siRNA-1 and PHF14 overexpression plasmid was analyzed by flow cytometry. D, Apoptosis of ESCC cells after cotransfection with SP4 siRNA-1 and PHF14-overexpression plasmid was analyzed by flow cytometry. E, PHF14 mRNA level was measured in ESCC cells after cotransfection with SP4 siRNA-1 and PHF14 overexpression vector. F, PHF14, Wnt3a, β-Catenin, CDK4, CDK6, Cyclin D1, and active caspase 3 expressions were measured after cotransfection with SP4 siRNA-1 and PHF14 overexpression vector. GAPDH was used as an internal reference. Each experiment was performed in triplicate. According to the data characteristics, quantitative data of A – E were analyzed by Student t test. *, P < 0.05.

Journal: Molecular Cancer Research

Article Title: SP4 Facilitates Esophageal Squamous Cell Carcinoma Progression by Activating PHF14 Transcription and Wnt/Β-Catenin Signaling

doi: 10.1158/1541-7786.MCR-22-0835

Figure Lengend Snippet: SP4 facilitates ESCC progression via modulating the Wnt/β-catenin signaling pathway by promoting PHF14 transcription. A, Viability of ESCC cells after cotransfection with SP4 siRNA-1 and PHF14 overexpression plasmid. B, Cell number of ESCC cells after cotransfection with SP4 siRNA-1 and PHF14 overexpression plasmid. C, The cell cycle of ESCC cells after cotransfection with SP4 siRNA-1 and PHF14 overexpression plasmid was analyzed by flow cytometry. D, Apoptosis of ESCC cells after cotransfection with SP4 siRNA-1 and PHF14-overexpression plasmid was analyzed by flow cytometry. E, PHF14 mRNA level was measured in ESCC cells after cotransfection with SP4 siRNA-1 and PHF14 overexpression vector. F, PHF14, Wnt3a, β-Catenin, CDK4, CDK6, Cyclin D1, and active caspase 3 expressions were measured after cotransfection with SP4 siRNA-1 and PHF14 overexpression vector. GAPDH was used as an internal reference. Each experiment was performed in triplicate. According to the data characteristics, quantitative data of A – E were analyzed by Student t test. *, P < 0.05.

Article Snippet: The GAPDH (60004-1-Ig), β-catenin (51067-2-AP), PHF14 (ProteinTech, catalog no. 24787–1-AP, RRID: AB_2879724), and mouse IgG (ProteinTech, catalog no. B900620, RRID: AB_2883054) antibodies were purchased from ProteinTech Group.

Techniques: Cotransfection, Over Expression, Plasmid Preparation, Flow Cytometry

Wnt/β-Catenin signaling pathway is downstream of SP4/PHF14. A, Viability of ESCC cells after treatment with Vector, ov-SP4, ov-SP4+LGK974. B, Cell number of ESCC cells after treatment with Vector, ov-SP4, ov-SP4+LGK974. C, The cell cycle of ESCC cells after treatment with vector, ov-SP4, ov-SP4+LGK974. D, Apoptosis of ESCC cells after treatment with vector, ov-SP4, ov-SP4+LGK974. E, Wnt3a, β-catenin expressions were measured after treatment with Vector, ov-SP4, ov-SP4+LGK974. GAPDH was used as an internal reference. F, PHF14, Wnt3a, β-catenin, CDK4, CDK6, Cyclin D1, and active caspase-3 expressions were measured after treatment with Vector, ov-SP4, ov-SP4+LGK974. GAPDH was used as an internal reference. Each experiment was performed in triplicate. According to the data characteristics, quantitative data of A – D were analyzed by Student t test. *, P < 0.05.

Journal: Molecular Cancer Research

Article Title: SP4 Facilitates Esophageal Squamous Cell Carcinoma Progression by Activating PHF14 Transcription and Wnt/Β-Catenin Signaling

doi: 10.1158/1541-7786.MCR-22-0835

Figure Lengend Snippet: Wnt/β-Catenin signaling pathway is downstream of SP4/PHF14. A, Viability of ESCC cells after treatment with Vector, ov-SP4, ov-SP4+LGK974. B, Cell number of ESCC cells after treatment with Vector, ov-SP4, ov-SP4+LGK974. C, The cell cycle of ESCC cells after treatment with vector, ov-SP4, ov-SP4+LGK974. D, Apoptosis of ESCC cells after treatment with vector, ov-SP4, ov-SP4+LGK974. E, Wnt3a, β-catenin expressions were measured after treatment with Vector, ov-SP4, ov-SP4+LGK974. GAPDH was used as an internal reference. F, PHF14, Wnt3a, β-catenin, CDK4, CDK6, Cyclin D1, and active caspase-3 expressions were measured after treatment with Vector, ov-SP4, ov-SP4+LGK974. GAPDH was used as an internal reference. Each experiment was performed in triplicate. According to the data characteristics, quantitative data of A – D were analyzed by Student t test. *, P < 0.05.

Article Snippet: The GAPDH (60004-1-Ig), β-catenin (51067-2-AP), PHF14 (ProteinTech, catalog no. 24787–1-AP, RRID: AB_2879724), and mouse IgG (ProteinTech, catalog no. B900620, RRID: AB_2883054) antibodies were purchased from ProteinTech Group.

Techniques: Plasmid Preparation