Journal: Animals : an Open Access Journal from MDPI

Article Title: The Efficacy of a Ferric Sillen Core-Linked Polymer in Suppressing the Pathogenicity of Campylobacter jejuni

doi: 10.3390/ani14213150

Figure Lengend Snippet: The primer sequences used to amplify the target and internal control genes from treated and untreated C. jejuni cDNA by RT-qPCR.

Article Snippet: C. jejuni (DSMZ, Braunschweig, Germany, 24306) suspension was prepared from a liquid stock obtained from DSMZ.

Techniques: Control, Sequencing

Journal: Animals : an Open Access Journal from MDPI

Article Title: The Efficacy of a Ferric Sillen Core-Linked Polymer in Suppressing the Pathogenicity of Campylobacter jejuni

doi: 10.3390/ani14213150

Figure Lengend Snippet: The inhibition of C. jejuni growth using an FSCLP. Cells were incubated for 48 h with the FSCLP at a range of Fe concentrations. Data are expressed as the mean with standard deviation (SD) of triplicate samples, with negative values adjusted to 0% to ensure biological accuracy. Statistically significant differences were determined to the corresponding positive control by one-way ANOVA followed by Dunnett’s test (denoted by * p < 0.05, ** p < 0.01, and *** p < 0.001).

Article Snippet: C. jejuni (DSMZ, Braunschweig, Germany, 24306) suspension was prepared from a liquid stock obtained from DSMZ.

Techniques: Inhibition, Incubation, Standard Deviation, Positive Control

Journal: Animals : an Open Access Journal from MDPI

Article Title: The Efficacy of a Ferric Sillen Core-Linked Polymer in Suppressing the Pathogenicity of Campylobacter jejuni

doi: 10.3390/ani14213150

Figure Lengend Snippet: Analysis of C. jejuni gene expression when grown in presence of FSCLP.

Article Snippet: C. jejuni (DSMZ, Braunschweig, Germany, 24306) suspension was prepared from a liquid stock obtained from DSMZ.

Techniques: Expressing

Journal: Animals : an Open Access Journal from MDPI

Article Title: The Efficacy of a Ferric Sillen Core-Linked Polymer in Suppressing the Pathogenicity of Campylobacter jejuni

doi: 10.3390/ani14213150

Figure Lengend Snippet: The influence of the FSCLP on C. jejuni adhesion to and the invasion of IPEC-J2 cell monolayers. Statistically significant differences were determined relative to the corresponding positive control (with no treatment) by one-way ANOVA followed by Dunnett’s test (denoted by ** p < 0.01). n = 3 for the adhesion and invasion assays.

Article Snippet: C. jejuni (DSMZ, Braunschweig, Germany, 24306) suspension was prepared from a liquid stock obtained from DSMZ.

Techniques: Positive Control

Journal: Antimicrobial Agents and Chemotherapy

Article Title: The point mutation A1387G in the 16S rRNA gene confers aminoglycoside resistance in Campylobacter jejuni and Campylobacter coli

doi: 10.1128/aac.00833-24

Figure Lengend Snippet: MIC values of the donor C. coli BfR-CA-15687, wild-type recipient isolates, and transformant strains for aminoglycosides ^a

Article Snippet: In addition, reference strains C. jejuni NCTC 11168 , 81-176 , and DSM 4688 (DSMZ—German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany) as well as C. coli strain 2012-70-443-2 (Technical University of Denmark, Lyngby, Denmark) were used.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: The point mutation A1387G in the 16S rRNA gene confers aminoglycoside resistance in Campylobacter jejuni and Campylobacter coli

doi: 10.1128/aac.00833-24

Figure Lengend Snippet: The transformation rates of APR R -GEN R -KAN R -TOB R resistance determinant using gDNA of BfR-CA-15687 was ~2.5 log 10 lower (blue bars) than transformation of the control rpsL A128G point mutation using gDNA of BfR-CA-14430-strep leading to STR R (turquoise bars). The sensitive wild-type strains C. jejuni 81-176, C. coli BfR-CA-11057, and C. coli BfR-CA-14856 were transformed with 1 µg/mL gDNA. Transformation rates were assessed from the ratio of resistant transformants relative to CFU on non-selective Columbia blood agar. The data stem from at least three independent experiments, with error bars representing standard deviation.

Article Snippet: In addition, reference strains C. jejuni NCTC 11168 , 81-176 , and DSM 4688 (DSMZ—German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany) as well as C. coli strain 2012-70-443-2 (Technical University of Denmark, Lyngby, Denmark) were used.

Techniques: Transformation Assay, Control, Mutagenesis, Standard Deviation

Journal: Antimicrobial Agents and Chemotherapy

Article Title: The point mutation A1387G in the 16S rRNA gene confers aminoglycoside resistance in Campylobacter jejuni and Campylobacter coli

doi: 10.1128/aac.00833-24

Figure Lengend Snippet: Single colonies of transformants switched from a mixed A/G genotype at position 1387 in 16S rRNA to only G at higher TOB concentrations. Two representative transformant colonies (CFU 1 and CFU 2) of each C. jejuni 81–176-TF15687 and C. coli BfR-CA-11057-TF15687 after transformation were transferred to different concentrations of TOB and in parallel on non-selective ColbA. Sanger sequencing revealed two populations of resistant transformants—either harboring base G upon transformation or a mixture of bases A and G at position 1387 in the 16S rRNA genes (marked with black arrows), which changed to only G at higher TOB concentrations. The base color code of the Sanger sequences is indicated below the chromatograms. TOB, tobramycin; wt, wild-type.

Article Snippet: In addition, reference strains C. jejuni NCTC 11168 , 81-176 , and DSM 4688 (DSMZ—German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany) as well as C. coli strain 2012-70-443-2 (Technical University of Denmark, Lyngby, Denmark) were used.

Techniques: Transformation Assay, Sequencing

Journal: Antimicrobial Agents and Chemotherapy

Article Title: The point mutation A1387G in the 16S rRNA gene confers aminoglycoside resistance in Campylobacter jejuni and Campylobacter coli

doi: 10.1128/aac.00833-24

Figure Lengend Snippet: A transformant harboring a mixed A/G genotype at position 1387 in 16S rRNA reverted to a sensitive phenotype after 15 passages ( A ). In contrast, a transformant with only G at position 1387 maintained resistance even after 45 passages ( B ). Transformants of C. jejuni 81-176-TF15687 were passaged on non-selective ColbA. After the indicated number of passages, the transformant culture was diluted and spread on non-selective ColbA plates in order to obtain single colonies. Subsequently, colony material was transferred on plates with different concentrations of TOB by stamping with velvet cloth. Photographs of colony patterns on each plate were captured after the indicated number of passages. Sanger sequences are shown after passage 1 (fresh transformant) and after repeated subculturing. After passaging, a colony was taken from non-selective ColbA for Sanger sequencing.

Article Snippet: In addition, reference strains C. jejuni NCTC 11168 , 81-176 , and DSM 4688 (DSMZ—German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany) as well as C. coli strain 2012-70-443-2 (Technical University of Denmark, Lyngby, Denmark) were used.

Techniques: Subculturing Assay, Passaging, Sequencing