Journal: Science Advances

Article Title: Elucidating tissue and subcellular specificity of the entire SUMO network reveals how stress responses are fine-tuned in a eukaryote

doi: 10.1126/sciadv.adw9153

Figure Lengend Snippet: ( A ) Organization of pMDS1 vector showing reporters for transcription [mTurquoise (mTurQ)], translation (C-terminal mVenus), and a 2A self-cleaving peptide. ( B ) Organization of pMDS2 vector showing reporters for transcription (mTurQ), translation (N-terminal mVenus), and a 2A self-cleaving peptide. ( C ) Confocal image of pMDS1_ SHR pro: SHR:mVenus:mTurQ showing gene expression (mTurQ) in the stele region and protein (mVenus) translocating to endodermis in the root meristem. ( D ) Confocal image of pMDS2_ VAM3 pro:mVenus: VAM3:mTurQ showing subcellular expression (mTurQ) in the nucleus and protein (mVenus) moving to the vacuole in the root epidermis. Red channel shows mCherry expression. nu, nucleus; vac, vacuole; *, endodermis of root meristem. Scale bar, 10 μM. ( E ) Immunoblot of pSHR::SHR pMDS1 showing efficient ribosomal skipping induced by the 2A peptides. Bands corresponding to mTurquoise are labeled as mTurQ. ( F ) Immunoblot of pVAM3::VAM3 pMDS2 showing efficient ribosomal skipping induced by the 2A peptides. Bands corresponding to mTurquoise are labeled as mTurQ.

Article Snippet: In addition, pMDS1 (239460) and pMDS2 (239459) vectors are available through Addgene.

Techniques: Plasmid Preparation, Gene Expression, Expressing, Western Blot, Labeling