Journal: Applied and Environmental Microbiology

Article Title: A Phage Lysin Fused to a Cell-Penetrating Peptide Kills Intracellular Methicillin-Resistant Staphylococcus aureus in Keratinocytes and Has Potential as a Treatment for Skin Infections in Mice

doi: 10.1128/AEM.00380-18

Figure Lengend Snippet: The various strains used in this study and lytic activity of JDlys and CPP-JDlys

Article Snippet: As shown in , JDlys was able to inhibit the growth of Staphylococcus strains from different sources and of different multilocus sequence typing (MLST) types. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species Strain MLST type MIC (μg/ml) Source a Jdlys CPP Tat -JDlys CPP Ant -JDlys CPP TP10 -JDlys Methicillin-resistant Staphylococcus aureus USA300 ST b 8 20 40 160 160 III MS5 ST 5 10 10 160 160 I MS6 ST 5 20 40 320 320 I MS8 ST 9 20 20 — c 320 I MS10 ST 239 80 80 — — I MS11 ST 9 20 40 320 320 I MS15 ST 5 20 40 320 160 I MS16 ST 9 20 40 320 320 I MS17 ST 398 80 80 — — I MS19 ST 9 10 20 320 320 I MS20 ST 398 20 20 — 160 I MS21 ST 398 20 40 — — I Methicillin-susceptible Staphylococcus aureus ATCC 25923 ST 243 20 40 160 160 III ATCC 29213 ST 5 40 40 320 320 III S1 ST 398 80 80 — — I S2 ST 239 20 20 160 160 I S4 ST 239 20 20 320 160 I S5 ST 398 10 20 160 160 I SH-5 ST 9 20 20 320 160 II SH-6 ST 9 40 40 — 320 II Staphylococcus sciuri Sci-3 40 40 320 320 II Staphylococcus saprophyticus Sap-1 40 80 — — II Staphylococcus caseolyticus Cas-1 80 80 320 320 II Open in a separate window a I, clinically isolated pathogenic strains; II, strains isolated from milk samples of dairy cows with mastitis; III, purchased from the American Type Culture Collection. b ST, sequence type. c —, no antimicrobial activity.

Techniques: Activity Assay