streptomyces coelicolor a3 2  (ATCC)

Journal: PLoS ONE

Article Title: Heterologous Expression of the Thiopeptide Antibiotic GE2270 from Planobispora rosea ATCC 53733 in Streptomyces coelicolor Requires Deletion of Ribosomal Genes from the Expression Construct

doi: 10.1371/journal.pone.0090499

Figure Lengend Snippet: A) Insert of 2F7 and 2F7cat comprising the GE2270 biosynthetic gene cluster ( pbt ), the tuf R gene coding for the GE2270-resistant EF-Tu, 25 adjacent ribosomal genes and rpoC coding for RNA polymerase β′-subunit (see ). B) Introduction of the inducible tcp830 promoter in front of the regulatory gene pbtR resulting in cosmid pbtKA01. C) For the construction of pbtKA02 the tcp830 promoter was introduced in front of pbtG1 under loss of pbtR . D) Replacement of 22 genes encoding ribosomal proteins with an apramycin resistance cassette ( aac(3)IV ) resulted in cosmid pbtCK01. E) pbtCK02 was constructed by introduction of the constitutive promoter ermE * and associated replacement of the ribosomal genes rpsL , rpsG and fusA with a hygromycin resistance cassette ( hyg ). F–H) Introduction of the inducible tcp830 promoter at three distinct positions in each case followed by subsequent removal of the employed aac(3)IV cassette. F) tcp830 introduced in front of the regulatory gene pbtR resulting in cosmids pbtCK03. G) tcp830 introduced in front of pbtG1 under loss of pbtR resulting in cosmid pbtCK04 and H) tcp830 introduced in front of the structural gene pbt A resulting in cosmid pbtCK05. I) negative control cosmid pbtCK08 was constructed by replacement of the pbt biosynthetic genes of cosmid 2F7cat by an apramycin resistance cassette ( aac(3)IV ). For each construct, the efficiency of conjugal transfer into Streptomyces coelicolor M1146 is expressed as number of exconjugants per 1 million spores.

Article Snippet: 16S rRNA sequences of selected actinomycetes were obtained from GenBank, despite Streptomyces coelicolor A3(2) ( http://strepdb.streptomyces.org.uk ) and Planobispora rosea ATCC 53733 (Naicons, see ), Catenulispora acidiphila DSM 44928 (accession number (acc.

Techniques: Construct, Negative Control

Journal: PLoS ONE

Article Title: Heterologous Expression of the Thiopeptide Antibiotic GE2270 from Planobispora rosea ATCC 53733 in Streptomyces coelicolor Requires Deletion of Ribosomal Genes from the Expression Construct

doi: 10.1371/journal.pone.0090499

Figure Lengend Snippet: A) GE2270A production of S. coelicolor M1146 strains containing different constructs of the GE2270 biosynthetic gene cluster (see Figure 3). The amount of GE2270A was determined after 8 days of cultivation in 24-square deepwell plates [28]. B) GE2270B1 production in S. coelicolor M1146(pbtCK05). Values are mean ± SEM from triplicated cultivation of three individual exconjugants each.

Article Snippet: 16S rRNA sequences of selected actinomycetes were obtained from GenBank, despite Streptomyces coelicolor A3(2) ( http://strepdb.streptomyces.org.uk ) and Planobispora rosea ATCC 53733 (Naicons, see ), Catenulispora acidiphila DSM 44928 (accession number (acc.

Techniques: Construct

Journal: BMC Microbiology

Article Title: Identification of a novel anti-σ E factor in Neisseria meningitidis

doi: 10.1186/1471-2180-10-164

Figure Lengend Snippet: Sequence alignment of orthologues of NMB2145 as found in other neisserial species and the ZAS containing anti-σ factors RsrA of S. coelicolor and ChrR of R. sphaeriodes . Numbers at the end of each sequence represent the total length of the protein and bracketed numbers show the number of residues not shown in the alignment of RsrA and ChrR with NMB2145. The ZAS motif (Hisx 3 Cysx 2 Cys) is indicated in yellow, the additional zinc ligand is indicated in red. Conserved residues of neisserial NMB2145 orthologues are indicated in green. Protein IDs or genomic coordinates (in case of missing protein annotation) are indicated on the right. Details regarding strains of which sequences were obtained are listed in the Materials and Methods section.

Article Snippet: Sequences from the following strains (with Genbank ID) were downloaded for comparative aligments: N.meningitidis_MC58 ( AE002098 ); N.meningitidis _FAM18 ( AM421808 ); N.meningitidis _053442 ( CP000381 ); N.meningitidis _Z2491 ( AL157959 ); N.gonorrhoeae _FA1090 ( AE004969 ); N.gonorrhoeae _NCCP11945 ( CP001050 ); N.cinerea _ATCC_14685 ( ACDY00000000 ); N.flavescens_ NRL30031/H210 ( ACEN00000000 ); N.lactamica_ ATCC_23970 ( ACEQ00000000 ); N.subflava _NJ9703 ( ACEO00000000 ); N.sicca _ATCC_29256 ( ACKO00000000 ); N.mucosa_ ATCC_25996 ( ACDX00000000 ); Streptomyces coelicolor _A3(2) ( AL645882 ); Rhodobacter sphaeroides _ATCC_17025 ( CP000661 ).

Techniques: Sequencing

Journal: World Journal of Microbiology & Biotechnology

Article Title: A bioprocess perspective on the production of secondary metabolites by Streptomyces in submerged co-cultures

doi: 10.1007/s11274-021-03141-z

Figure Lengend Snippet: The bioprocess-related summary of the studies reporting the induction or enhancement of secondary metabolites production by various species representing the Streptomyces genus in submerged co-cultures

Article Snippet: Luti and Mavituna ( ) , Undecylprodigiosin , Enhanced (2.6-fold increase in concentration) , Streptomyces coelicolor A3 (2) , Bacillus subtilis ATCC 6633 , Glucose, NaNO 3 , NaCl, Na 2 SO 4 , K 2 HPO 4 , TRIS base, MgSO 4 ·7H 2 O, ZnSO 4, trace elements solution , 7.2 , 500-ml shake flasks , Shaking at 200 rpm; temperature 30 °C , Vegetative cells , Co-inoculation of sterile medium , In preculture broth (producer); in saline solution (partner).

Techniques: Concentration Assay