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Cayman Chemical il 6 elisa kits
Inflammation in aged mice. Levels of TNF‐α (a) <t>and</t> <t>IL‐6</t> (b) in sera from young (10 weeks) and aged (20–22 months) C57BL/6 mice as determined by <t>ELISA;</t> untreated: n = 12 (young) or n = 15 (aged), LPS (5 mg/kg BW, 4 hr): n = 6. (c) PMs obtained from young and aged mice were stimulated with LPS (100 ng/ml, 4 hr), and TNF was quantified in the supernatants by bioassay ( n = 4, triplicates). (d) H 2 O 2 production was measured in PM supernatants by homovanillic acid assay (young: n = 5, aged: n = 6). (e, f) ERK phosphorylation after LPS treatment (100 ng/ml) was examined by Western blot. Total ERK (tERK) and tubulin served as loading controls. (e) One representative blot out of six is shown. (f) pERK1/2 signal intensities were normalized to total ERK1/2. Values for untreated cells from young mice were set as 1 ( n = 6 in replicates). (g) Gene expression in PMs from young and aged mice was determined by qPCR and normalized against the housekeeping gene (Ppia). Values for PMs from young mice were set as 1 (n = 14). Box plots show the 25–75th percentiles (box), mean (square), median (line), and SD (whiskers). Bars show the mean ± SEM , and circles within bars indicate the mean of each individual experiment. * p < .05, ** p < .01, *** p < .001 by two‐tailed t test (a–c), Mann–Whitney U test (d, g), or ANOVA with Bonferroni's post hoc test (f)
Il 6 Elisa Kits, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Altered glucocorticoid metabolism represents a feature of macroph‐aging"

Article Title: Altered glucocorticoid metabolism represents a feature of macroph‐aging

Journal: Aging Cell

doi: 10.1111/acel.13156

Inflammation in aged mice. Levels of TNF‐α (a) and IL‐6 (b) in sera from young (10 weeks) and aged (20–22 months) C57BL/6 mice as determined by ELISA; untreated: n = 12 (young) or n = 15 (aged), LPS (5 mg/kg BW, 4 hr): n = 6. (c) PMs obtained from young and aged mice were stimulated with LPS (100 ng/ml, 4 hr), and TNF was quantified in the supernatants by bioassay ( n = 4, triplicates). (d) H 2 O 2 production was measured in PM supernatants by homovanillic acid assay (young: n = 5, aged: n = 6). (e, f) ERK phosphorylation after LPS treatment (100 ng/ml) was examined by Western blot. Total ERK (tERK) and tubulin served as loading controls. (e) One representative blot out of six is shown. (f) pERK1/2 signal intensities were normalized to total ERK1/2. Values for untreated cells from young mice were set as 1 ( n = 6 in replicates). (g) Gene expression in PMs from young and aged mice was determined by qPCR and normalized against the housekeeping gene (Ppia). Values for PMs from young mice were set as 1 (n = 14). Box plots show the 25–75th percentiles (box), mean (square), median (line), and SD (whiskers). Bars show the mean ± SEM , and circles within bars indicate the mean of each individual experiment. * p < .05, ** p < .01, *** p < .001 by two‐tailed t test (a–c), Mann–Whitney U test (d, g), or ANOVA with Bonferroni's post hoc test (f)
Figure Legend Snippet: Inflammation in aged mice. Levels of TNF‐α (a) and IL‐6 (b) in sera from young (10 weeks) and aged (20–22 months) C57BL/6 mice as determined by ELISA; untreated: n = 12 (young) or n = 15 (aged), LPS (5 mg/kg BW, 4 hr): n = 6. (c) PMs obtained from young and aged mice were stimulated with LPS (100 ng/ml, 4 hr), and TNF was quantified in the supernatants by bioassay ( n = 4, triplicates). (d) H 2 O 2 production was measured in PM supernatants by homovanillic acid assay (young: n = 5, aged: n = 6). (e, f) ERK phosphorylation after LPS treatment (100 ng/ml) was examined by Western blot. Total ERK (tERK) and tubulin served as loading controls. (e) One representative blot out of six is shown. (f) pERK1/2 signal intensities were normalized to total ERK1/2. Values for untreated cells from young mice were set as 1 ( n = 6 in replicates). (g) Gene expression in PMs from young and aged mice was determined by qPCR and normalized against the housekeeping gene (Ppia). Values for PMs from young mice were set as 1 (n = 14). Box plots show the 25–75th percentiles (box), mean (square), median (line), and SD (whiskers). Bars show the mean ± SEM , and circles within bars indicate the mean of each individual experiment. * p < .05, ** p < .01, *** p < .001 by two‐tailed t test (a–c), Mann–Whitney U test (d, g), or ANOVA with Bonferroni's post hoc test (f)

Techniques Used: Enzyme-linked Immunosorbent Assay, Acid Assay, Western Blot, Expressing, Two Tailed Test, MANN-WHITNEY


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atcc 23615  (ATCC)


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    ATCC atcc 23615
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    atcc 23615  (ATCC)


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    ATCC atcc 23615
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    h r pd f 23615 octasa step 2b bnf ad gast nursing aw indd  (MA Healthcare Ltd)

     
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    MA Healthcare Ltd h r pd f 23615 octasa step 2b bnf ad gast nursing aw indd
    H R Pd F 23615 Octasa Step 2b Bnf Ad Gast Nursing Aw Indd, supplied by MA Healthcare Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cayman Chemical il 6 elisa kits
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