Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
Article Title: Expression microarray analysis identifies novel epithelial-derived protein markers in eosinophilic esophagitis.
doi: 10.1038/modpathol.2013.41
Figure Lengend Snippet: Figure 4 Immunohistochemistry results of FLG, SLURP1 and CRISP3. (a) Normal controls; (b) eosinophilic esophagitis; (c) eosinophilic esophagitis after treatment; (d) reflux. FLG expression was present in the cytoplasm of maturing squamous cells of control samples. Eosinophilic esophagitis biopsies show loss of FLG expression with partial recovery after treatment. Reflux biopsies do not show loss of FLG expression. SLURP1 staining was present in cytoplasm of intermediate and mature squamous epithelial cells of control cases. Expression of SLURP1 is minimal in eosinophilic esophagitis and decreased in reflux cases. CRISP3 expression is strong in the cytoplasm of basal cells and peripapillary cells and in mature superficial squamous cells of control samples. Expression of CRISP3 is decreased in eosinophilic esophagitis. Eosinophilic esophagitis after treatment and reflux cases show CRISP3 expression limited to the basal cells and peripapillary cells but with no expression in cytoplasm of mature squamous cells.
Article Snippet: The Dako Envision Plus Kit (Dako North America, Carpinteria, CA, USA) was used to perform the polymer-horseradish peroxidase-based IHC using the following antibodies: SLURP1 (Clone 569317; R&D Systems, Minneapolis, MN, USA; 1:100 dilution), CRISP3 (clone 295203, R&D Systems; 1:100 dilution), FLG (SPM181, Abcam, Cambridge, MA, USA; 1:25 dilution), ALOX15 (11-K, Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1:100 dilution) and TNFAIP6 (FL-277, Santa Cruz Biotechnology; 1:200 dilution).
Techniques: Immunohistochemistry, Reflux, Expressing, Control, Staining