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Figure 2. Comparison of Podosomes and Podosome-like Structures Comparison of podosomes formed by resting human macrophages (left) and podosome-like structures formed during frustrated phagocytosis of IgG-opsonized glass (center-left). Stained for active b-2 integrins, <t>talin,</t> vinculin, paxillin, myosin IIa, and ArpC2. Insets show merged images of F-actin (red) and the specified protein (green). Scale bars, 5 mm and 1 mm (insets). Right panels are line scans for F-actin (red) and the specified protein (green) from indicated region highlighted in yellow on corresponding image on left.
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Figure 2. Comparison of Podosomes and Podosome-like Structures Comparison of podosomes formed by resting human macrophages (left) and podosome-like structures formed during frustrated phagocytosis of IgG-opsonized glass (center-left). Stained for active b-2 integrins, <t>talin,</t> vinculin, paxillin, myosin IIa, and ArpC2. Insets show merged images of F-actin (red) and the specified protein (green). Scale bars, 5 mm and 1 mm (insets). Right panels are line scans for F-actin (red) and the specified protein (green) from indicated region highlighted in yellow on corresponding image on left.
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Figure 2. Comparison of Podosomes and Podosome-like Structures Comparison of podosomes formed by resting human macrophages (left) and podosome-like structures formed during frustrated phagocytosis of IgG-opsonized glass (center-left). Stained for active b-2 integrins, <t>talin,</t> vinculin, paxillin, myosin IIa, and ArpC2. Insets show merged images of F-actin (red) and the specified protein (green). Scale bars, 5 mm and 1 mm (insets). Right panels are line scans for F-actin (red) and the specified protein (green) from indicated region highlighted in yellow on corresponding image on left.
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Figure 2. Comparison of Podosomes and Podosome-like Structures Comparison of podosomes formed by resting human macrophages (left) and podosome-like structures formed during frustrated phagocytosis of IgG-opsonized glass (center-left). Stained for active b-2 integrins, <t>talin,</t> vinculin, paxillin, myosin IIa, and ArpC2. Insets show merged images of F-actin (red) and the specified protein (green). Scale bars, 5 mm and 1 mm (insets). Right panels are line scans for F-actin (red) and the specified protein (green) from indicated region highlighted in yellow on corresponding image on left.
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Figure 2. Comparison of Podosomes and Podosome-like Structures Comparison of podosomes formed by resting human macrophages (left) and podosome-like structures formed during frustrated phagocytosis of IgG-opsonized glass (center-left). Stained for active b-2 integrins, <t>talin,</t> vinculin, paxillin, myosin IIa, and ArpC2. Insets show merged images of F-actin (red) and the specified protein (green). Scale bars, 5 mm and 1 mm (insets). Right panels are line scans for F-actin (red) and the specified protein (green) from indicated region highlighted in yellow on corresponding image on left.
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Figure 2. Comparison of Podosomes and Podosome-like Structures Comparison of podosomes formed by resting human macrophages (left) and podosome-like structures formed during frustrated phagocytosis of IgG-opsonized glass (center-left). Stained for active b-2 integrins, talin, vinculin, paxillin, myosin IIa, and ArpC2. Insets show merged images of F-actin (red) and the specified protein (green). Scale bars, 5 mm and 1 mm (insets). Right panels are line scans for F-actin (red) and the specified protein (green) from indicated region highlighted in yellow on corresponding image on left.

Journal: Developmental cell

Article Title: Dynamic Podosome-Like Structures in Nascent Phagosomes Are Coordinated by Phosphoinositides.

doi: 10.1016/j.devcel.2019.05.028

Figure Lengend Snippet: Figure 2. Comparison of Podosomes and Podosome-like Structures Comparison of podosomes formed by resting human macrophages (left) and podosome-like structures formed during frustrated phagocytosis of IgG-opsonized glass (center-left). Stained for active b-2 integrins, talin, vinculin, paxillin, myosin IIa, and ArpC2. Insets show merged images of F-actin (red) and the specified protein (green). Scale bars, 5 mm and 1 mm (insets). Right panels are line scans for F-actin (red) and the specified protein (green) from indicated region highlighted in yellow on corresponding image on left.

Article Snippet: Vinculin (MAB3574) and Arpc2 (07-227) antibodies were from EMDMillipore, myosin IIa nonmuscle (M8064) antibody from Sigma Aldrich, talin (8D4) antibody from Novus Biologicals, active b-2 integrin (MEM-148) antibody from Bio-Rad Laboratories Inc., WASP (sc-13139) from Santa Cruz Biotechnology, and paxillin (610051) antibody from BD Transduction Laboratories.

Techniques: Comparison, Staining