m h ubi  (ATCC)

Journal: Molecular Medicine Reports

Article Title: Simulated microgravity hampers Notch signaling in the fight against myocardial ischemia-reperfusion injury

doi: 10.3892/mmr.2018.8489

Figure Lengend Snippet: Expression of hepatic Notch1 at different stages of rat development. Frozen sections (magnification, ×60) of liver from embryonic, neonatal and adult rats were stained for the extracellular domain of Notch1 (green), membrane (WGA, red) and nucleus (Hoechst, blue). The size of hepatocytes increased over developmental time, and the intensity of Notch1 expression decreased from the embryonic to the adult stage. The enlarged area (magnification, ×200) demonstrates the ring-shaped distribution of Notch1 around hepatocytes, which was particularly enhanced in the embryonic and neonatal stages. WGA, wheat germ agglutinin.

Article Snippet: Primary antibodies against Notch1 extracellular domain (cat. no. sc-23299) and JAG1 (cat. no. sc-8303) and connexin 43 (Cx43; cat. no. sc-13558) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA), and anti-β-actin (cat. no. A5441) was purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).

Techniques: Expressing, Staining, Membrane

Journal: Molecular Medicine Reports

Article Title: Simulated microgravity hampers Notch signaling in the fight against myocardial ischemia-reperfusion injury

doi: 10.3892/mmr.2018.8489

Figure Lengend Snippet: Expression of hepatic JAG1 in different stages of rat development. Frozen sections (magnification, ×60) of liver from embryonic, neonatal and adult rats were stained for JAG1 (green), membrane stain (WGA, red) and nucleus (Hoechst, blue). The intensity of JAG1 expression decreased, similar to Notch1 expression. The enlarged area (magnification, ×200) indicates the ring-shaped distribution of JAG1 around hepatocytes. JAG1, Jagged1.

Article Snippet: Primary antibodies against Notch1 extracellular domain (cat. no. sc-23299) and JAG1 (cat. no. sc-8303) and connexin 43 (Cx43; cat. no. sc-13558) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA), and anti-β-actin (cat. no. A5441) was purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).

Techniques: Expressing, Staining, Membrane

Journal: Molecular Medicine Reports

Article Title: Simulated microgravity hampers Notch signaling in the fight against myocardial ischemia-reperfusion injury

doi: 10.3892/mmr.2018.8489

Figure Lengend Snippet: Notch1 expression in rat heart and liver at different stages. (A) Frozen sections of heart and liver from embryonic, neonatal and adult rats were stained for the extracellular domain of Notch1 (green) and nucleus (Hoechst, blue). As the tissues developed naturally from the embryonic stage towards the adult stage, the constituent cells of adult groups grew larger than the other groups. Therefore, the tissues of adult rats achieved a lower density than those of embryonic and neonatal rats did. All displayed sections were captured under the same magnification (×60). (B) Quantification of the relative fluorescence intensity of Notch1 expression. The values are shown as fold change vs. the expression levels of Notch1 in adult liver group. Data are presented as the mean ± standard error of the mean of three experiments. **P<0.01 vs. the adult liver group; ## P<0.01 vs. the embryonic liver group; && P<0.01 vs. the embryonic heart group.

Article Snippet: Primary antibodies against Notch1 extracellular domain (cat. no. sc-23299) and JAG1 (cat. no. sc-8303) and connexin 43 (Cx43; cat. no. sc-13558) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA), and anti-β-actin (cat. no. A5441) was purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).

Techniques: Expressing, Staining, Fluorescence

Journal: Molecular Medicine Reports

Article Title: Simulated microgravity hampers Notch signaling in the fight against myocardial ischemia-reperfusion injury

doi: 10.3892/mmr.2018.8489

Figure Lengend Snippet: Notch1 protein expression is increased in the border zone 7 days following ischemia-reperfusion injury. (A) Representative western blot images from CON-IR-7d rats indicating the expression levels of Notch1 in the three zones of the heart; β-actin was used as a loading control. (B) Quantification of Notch1 expression levels from Part (A) normalized to β-actin expression. Data are presented as the mean ± standard error of the mean of three experiments. **P<0.01 vs. remote zone.

Article Snippet: Primary antibodies against Notch1 extracellular domain (cat. no. sc-23299) and JAG1 (cat. no. sc-8303) and connexin 43 (Cx43; cat. no. sc-13558) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA), and anti-β-actin (cat. no. A5441) was purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).

Techniques: Expressing, Western Blot, Control

Journal: Molecular Medicine Reports

Article Title: Simulated microgravity hampers Notch signaling in the fight against myocardial ischemia-reperfusion injury

doi: 10.3892/mmr.2018.8489

Figure Lengend Snippet: Notch1 expression is increased in the border zone in rat hearts following IR injury. (A) Frozen sections of adult rat hearts subjected to IR, with or without suspension, or sham operation were stained for the extracellular domain of Notch1 (green) and nucleus (Hoechst, blue). All sections were also counterstained with Cx43 (data not shown) to distinguish the different zones of the infarcted hearts. (B) Quantification of the relative fluorescence intensity of Notch1 expression. The values are shown as fold changes vs. the expression levels of Notch1 in adult liver group indicated in . Data are presented as the mean ± standard error of the mean of three experiments. **P<0.01 vs. CON-Sham rats; ## P<0.01 vs. SUS-Sham rats; && P<0.01 vs. the border zone of CON rats. CON, control; Cx43, connexin 43; IR, ischemia reperfusion; SUS, tail-suspended.

Article Snippet: Primary antibodies against Notch1 extracellular domain (cat. no. sc-23299) and JAG1 (cat. no. sc-8303) and connexin 43 (Cx43; cat. no. sc-13558) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA), and anti-β-actin (cat. no. A5441) was purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).

Techniques: Expressing, Suspension, Staining, Fluorescence, Control

Journal: Canadian Journal of Gastroenterology & Hepatology

Article Title: Notch Signaling Pathway Is Inhibited in the Development of Barrett's Esophagus: An In Vivo and In Vitro Study

doi: 10.1155/2018/4149317

Figure Lengend Snippet: The sequences for the primers used for PCR.

Article Snippet: Notch1 , Santa Cruz (sc-23299) , 1 : 1000.

Techniques: Amplification

Journal: Canadian Journal of Gastroenterology & Hepatology

Article Title: Notch Signaling Pathway Is Inhibited in the Development of Barrett's Esophagus: An In Vivo and In Vitro Study

doi: 10.1155/2018/4149317

Figure Lengend Snippet: Antibodies used for western blotting.

Article Snippet: Notch1 , Santa Cruz (sc-23299) , 1 : 1000.

Techniques: Western Blot

Journal: Canadian Journal of Gastroenterology & Hepatology

Article Title: Notch Signaling Pathway Is Inhibited in the Development of Barrett's Esophagus: An In Vivo and In Vitro Study

doi: 10.1155/2018/4149317

Figure Lengend Snippet: Immunohistochemical results of Notch1.

Article Snippet: Notch1 , Santa Cruz (sc-23299) , 1 : 1000.

Techniques: Immunohistochemical staining, Paraffin-embedded Immunohistochemistry

Journal: Canadian Journal of Gastroenterology & Hepatology

Article Title: Notch Signaling Pathway Is Inhibited in the Development of Barrett's Esophagus: An In Vivo and In Vitro Study

doi: 10.1155/2018/4149317

Figure Lengend Snippet: Representative Notch1 immunohistochemical staining in samples with different histological alterations. Notch1 protein was mainly expressed in cytoplasm of esophageal cells. Intense staining of Notch1 was mainly present in normal esophagus, and metaplastic columnar cells mainly exhibited negative staining (arrows). Scale bar in the figure is 40 μ m. Magnified figures were shown in the right side.

Article Snippet: Notch1 , Santa Cruz (sc-23299) , 1 : 1000.

Techniques: Immunohistochemical staining, Staining, Negative Staining

Journal: Canadian Journal of Gastroenterology & Hepatology

Article Title: Notch Signaling Pathway Is Inhibited in the Development of Barrett's Esophagus: An In Vivo and In Vitro Study

doi: 10.1155/2018/4149317

Figure Lengend Snippet: Relative expression of Notch1 (a) and Hes1 (b) mRNA in normal esophagus and metaplastic esophagus. Significant differences were detected in Notch1 mRNA and Hes1 mRNA expression values between normal squamous mucosa and metaplastic tissue. ∗ p < 0.05, statistically significant difference.

Article Snippet: Notch1 , Santa Cruz (sc-23299) , 1 : 1000.

Techniques: Expressing

Journal: Canadian Journal of Gastroenterology & Hepatology

Article Title: Notch Signaling Pathway Is Inhibited in the Development of Barrett's Esophagus: An In Vivo and In Vitro Study

doi: 10.1155/2018/4149317

Figure Lengend Snippet: Effects of hydrochloric acid on Notch1, Hes1, K13, and MUC2 expressions. Cells were treated with different concentrations of hydrochloric acid (pH4, pH5, and pH6) for 24 h, 48 h, 72 h, and 96 h, respectively. (a–d) Notch1, Hes1, K13, and MUC2 mRNA expression levels in Het-1A cells exposing to different concentrations of hydrochloric acid for different time periods. ∗ p < 0.05, statistically significant difference. ∗∗ p < 0.01, statistically significant difference. ∗∗∗ p < 0.001, statistically significant difference. (e) Representative western blot results of Notch1, Hes1, K13, and MUC2 protein expressions in Het-1A cells treated with hydrochloric acid (pH5) for different time periods.

Article Snippet: Notch1 , Santa Cruz (sc-23299) , 1 : 1000.

Techniques: Expressing, Western Blot

Journal: Canadian Journal of Gastroenterology & Hepatology

Article Title: Notch Signaling Pathway Is Inhibited in the Development of Barrett's Esophagus: An In Vivo and In Vitro Study

doi: 10.1155/2018/4149317

Figure Lengend Snippet: Effects of deoxycholic acid on Notch1, Hes1, K13, and MUC2 expressions. Cells were treated with different concentrations of deoxycholic acid (300 umol/L, 500 umol/L and 1000 umol/L) for 24 h, 48 h, 72 h, and 96 h, respectively. (a–d) Notch1, Hes1, K13, and MUC2 mRNA expression levels in Het-1A cells exposing to different concentrations of deoxycholic acid for different time periods. ∗ p < 0.05, statistically significant difference. ∗∗ p < 0.01, statistically significant difference. ∗∗∗ p < 0.001, statistically significant difference. (e) Representative western blot results of Notch1, Hes1, K13, and MUC2 protein expressions in Het-1A cells treated with deoxycholic acid (500 umol/L) for different time periods.

Article Snippet: Notch1 , Santa Cruz (sc-23299) , 1 : 1000.

Techniques: Expressing, Western Blot

Journal: Canadian Journal of Gastroenterology & Hepatology

Article Title: Notch Signaling Pathway Is Inhibited in the Development of Barrett's Esophagus: An In Vivo and In Vitro Study

doi: 10.1155/2018/4149317

Figure Lengend Snippet: Effects of mixture of hydrochloric acid and deoxycholic acid on Notch1, Hes1, K13, and MUC2 expressions. Cells were treated with mixture of hydrochloric acid (pH = 5) and deoxycholic acid (500 umol/L) for 24 h, 48 h, 72 h, and 96 h, respectively. (a) Notch1, Hes1, K13, and MUC2 mRNA expression levels in Het-1A cells exposing to mixture of hydrochloric acid and deoxycholic acid for different time periods. ∗ p < 0.05, statistically significant difference. ∗∗ p < 0.01, statistically significant difference. ∗∗∗ p < 0.001, statistically significant difference. (b) Representative western blot results of Notch1, Hes1, K13, and MUC2 protein expressions in Het-1A cells treated with mixture of hydrochloric acid (pH = 5) and deoxycholic acid (500 umol/L) for different time periods.

Article Snippet: Notch1 , Santa Cruz (sc-23299) , 1 : 1000.

Techniques: Expressing, Western Blot

Journal: Canadian Journal of Gastroenterology & Hepatology

Article Title: Notch Signaling Pathway Is Inhibited in the Development of Barrett's Esophagus: An In Vivo and In Vitro Study

doi: 10.1155/2018/4149317

Figure Lengend Snippet: Effects of Notch signaling inhibition. Expressions of Notch1, Hes1, and K13 were significantly downregulated in cells transfected with Notch1-siRNA, in concert with significant increase of MUC2 expression. (a) Notch1, Hes1, K13, and MUC2 mRNA expression levels by RT-qPCR in Het-1A cells transfected with Notch1-siRNA. ∗∗ p < 0.01, statistically significant difference. ∗∗∗ p < 0.001, statistically significant difference. (b) Notch1, Hes1, K13, and MUC2 protein expressions by western blot analysis in Het-1A cells after Notch signaling inhibition by Notch1-siRNA.

Article Snippet: Notch1 , Santa Cruz (sc-23299) , 1 : 1000.

Techniques: Inhibition, Transfection, Expressing, Quantitative RT-PCR, Western Blot