tods 23 978 protein  (Agilent technologies)


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    Agilent technologies tods 23 978 protein
    SEC-MALS and electron microscopic analyses of TodS. A , SEC analysis of purified <t>TodS(23–978).</t> Eluted TodS was compared with the molecular mass standard markers β-amylase (223.8 kDa) and apoferritin (443 kDa). B , SEC-MALS analysis of TodS(23–978). Horizontal lines across the peaks indicate the calculated molecular mass of eluted TodS(23–978) ( red ), β-amylase ( blue ), and BSA ( black ). The inset shows SDS-PAGE of purified TodS(23–978) used for SEC-MALS analysis. C , electron microscopic image of gold-labeled TodS proteins. Ni-NTA-gold labeling of C-terminal His 6 -tagged TodS(43–978) proteins was employed to define the molecular arrangement of the TodS dimer. Shown is a negative-stained image showing paired circular gold particles ( yellow arrows ), suggesting a head-to-head dimer of TodS. Scale bar , 50 nm. D , molecular appearance of TodS(23–978) proteins. Electron microscopic images of apo-TodS ( top ), TodS treated with toluene ( middle ), and TodS treated with 1,2,4-TMB ( bottom ) were visualized by negative staining. Ten representative images were selected. Scale bar , 20 nm.
    Tods 23 978 Protein, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Molecular Insights into Toluene Sensing in the TodS/TodT Signal Transduction System"

    Article Title: Molecular Insights into Toluene Sensing in the TodS/TodT Signal Transduction System

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.718841

    SEC-MALS and electron microscopic analyses of TodS. A , SEC analysis of purified TodS(23–978). Eluted TodS was compared with the molecular mass standard markers β-amylase (223.8 kDa) and apoferritin (443 kDa). B , SEC-MALS analysis of TodS(23–978). Horizontal lines across the peaks indicate the calculated molecular mass of eluted TodS(23–978) ( red ), β-amylase ( blue ), and BSA ( black ). The inset shows SDS-PAGE of purified TodS(23–978) used for SEC-MALS analysis. C , electron microscopic image of gold-labeled TodS proteins. Ni-NTA-gold labeling of C-terminal His 6 -tagged TodS(43–978) proteins was employed to define the molecular arrangement of the TodS dimer. Shown is a negative-stained image showing paired circular gold particles ( yellow arrows ), suggesting a head-to-head dimer of TodS. Scale bar , 50 nm. D , molecular appearance of TodS(23–978) proteins. Electron microscopic images of apo-TodS ( top ), TodS treated with toluene ( middle ), and TodS treated with 1,2,4-TMB ( bottom ) were visualized by negative staining. Ten representative images were selected. Scale bar , 20 nm.
    Figure Legend Snippet: SEC-MALS and electron microscopic analyses of TodS. A , SEC analysis of purified TodS(23–978). Eluted TodS was compared with the molecular mass standard markers β-amylase (223.8 kDa) and apoferritin (443 kDa). B , SEC-MALS analysis of TodS(23–978). Horizontal lines across the peaks indicate the calculated molecular mass of eluted TodS(23–978) ( red ), β-amylase ( blue ), and BSA ( black ). The inset shows SDS-PAGE of purified TodS(23–978) used for SEC-MALS analysis. C , electron microscopic image of gold-labeled TodS proteins. Ni-NTA-gold labeling of C-terminal His 6 -tagged TodS(43–978) proteins was employed to define the molecular arrangement of the TodS dimer. Shown is a negative-stained image showing paired circular gold particles ( yellow arrows ), suggesting a head-to-head dimer of TodS. Scale bar , 50 nm. D , molecular appearance of TodS(23–978) proteins. Electron microscopic images of apo-TodS ( top ), TodS treated with toluene ( middle ), and TodS treated with 1,2,4-TMB ( bottom ) were visualized by negative staining. Ten representative images were selected. Scale bar , 20 nm.

    Techniques Used: Purification, SDS Page, Labeling, Staining, Negative Staining

    apo tods 23 978 protein  (Waters Corporation)


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    Waters Corporation apo tods 23 978 protein
    SEC-MALS and electron microscopic analyses of TodS. A , SEC analysis of purified <t>TodS(23–978).</t> Eluted TodS was compared with the molecular mass standard markers β-amylase (223.8 kDa) and apoferritin (443 kDa). B , SEC-MALS analysis of TodS(23–978). Horizontal lines across the peaks indicate the calculated molecular mass of eluted TodS(23–978) ( red ), β-amylase ( blue ), and BSA ( black ). The inset shows SDS-PAGE of purified TodS(23–978) used for SEC-MALS analysis. C , electron microscopic image of gold-labeled TodS proteins. Ni-NTA-gold labeling of C-terminal His 6 -tagged TodS(43–978) proteins was employed to define the molecular arrangement of the TodS dimer. Shown is a negative-stained image showing paired circular gold particles ( yellow arrows ), suggesting a head-to-head dimer of TodS. Scale bar , 50 nm. D , molecular appearance of TodS(23–978) proteins. Electron microscopic images of apo-TodS ( top ), TodS treated with toluene ( middle ), and TodS treated with 1,2,4-TMB ( bottom ) were visualized by negative staining. Ten representative images were selected. Scale bar , 20 nm.
    Apo Tods 23 978 Protein, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Molecular Insights into Toluene Sensing in the TodS/TodT Signal Transduction System * "

    Article Title: Molecular Insights into Toluene Sensing in the TodS/TodT Signal Transduction System *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.718841

    SEC-MALS and electron microscopic analyses of TodS. A , SEC analysis of purified TodS(23–978). Eluted TodS was compared with the molecular mass standard markers β-amylase (223.8 kDa) and apoferritin (443 kDa). B , SEC-MALS analysis of TodS(23–978). Horizontal lines across the peaks indicate the calculated molecular mass of eluted TodS(23–978) ( red ), β-amylase ( blue ), and BSA ( black ). The inset shows SDS-PAGE of purified TodS(23–978) used for SEC-MALS analysis. C , electron microscopic image of gold-labeled TodS proteins. Ni-NTA-gold labeling of C-terminal His 6 -tagged TodS(43–978) proteins was employed to define the molecular arrangement of the TodS dimer. Shown is a negative-stained image showing paired circular gold particles ( yellow arrows ), suggesting a head-to-head dimer of TodS. Scale bar , 50 nm. D , molecular appearance of TodS(23–978) proteins. Electron microscopic images of apo-TodS ( top ), TodS treated with toluene ( middle ), and TodS treated with 1,2,4-TMB ( bottom ) were visualized by negative staining. Ten representative images were selected. Scale bar , 20 nm.
    Figure Legend Snippet: SEC-MALS and electron microscopic analyses of TodS. A , SEC analysis of purified TodS(23–978). Eluted TodS was compared with the molecular mass standard markers β-amylase (223.8 kDa) and apoferritin (443 kDa). B , SEC-MALS analysis of TodS(23–978). Horizontal lines across the peaks indicate the calculated molecular mass of eluted TodS(23–978) ( red ), β-amylase ( blue ), and BSA ( black ). The inset shows SDS-PAGE of purified TodS(23–978) used for SEC-MALS analysis. C , electron microscopic image of gold-labeled TodS proteins. Ni-NTA-gold labeling of C-terminal His 6 -tagged TodS(43–978) proteins was employed to define the molecular arrangement of the TodS dimer. Shown is a negative-stained image showing paired circular gold particles ( yellow arrows ), suggesting a head-to-head dimer of TodS. Scale bar , 50 nm. D , molecular appearance of TodS(23–978) proteins. Electron microscopic images of apo-TodS ( top ), TodS treated with toluene ( middle ), and TodS treated with 1,2,4-TMB ( bottom ) were visualized by negative staining. Ten representative images were selected. Scale bar , 20 nm.

    Techniques Used: Purification, SDS Page, Labeling, Staining, Negative Staining

    tods 23 978 protein  (Agilent technologies)


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    Agilent technologies tods 23 978 protein
    Tods 23 978 Protein, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    apo tods 23 978 protein  (Waters Corporation)


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    Waters Corporation apo tods 23 978 protein
    Apo Tods 23 978 Protein, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    apo tods 23 978 protein  (Waters Corporation)


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    Waters Corporation apo tods 23 978 protein
    Apo Tods 23 978 Protein, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tods 23 978 protein  (Agilent technologies)


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    Agilent technologies tods 23 978 protein
    Tods 23 978 Protein, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Structured Review

    Routledge Ltd isbn 978 0 23 010459 4 caroline sarojini hart
    Isbn 978 0 23 010459 4 Caroline Sarojini Hart, supplied by Routledge Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    2010 isbn 978 3 941468 23 8 jörg walden und heinz grunze 55 seiten  (Verlag GmbH)

     
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    Verlag GmbH 2010 isbn 978 3 941468 23 8 jörg walden und heinz grunze 55 seiten
    2010 Isbn 978 3 941468 23 8 Jörg Walden Und Heinz Grunze 55 Seiten, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies tods 23 978 protein
    SEC-MALS and electron microscopic analyses of TodS. A , SEC analysis of purified <t>TodS(23–978).</t> Eluted TodS was compared with the molecular mass standard markers β-amylase (223.8 kDa) and apoferritin (443 kDa). B , SEC-MALS analysis of TodS(23–978). Horizontal lines across the peaks indicate the calculated molecular mass of eluted TodS(23–978) ( red ), β-amylase ( blue ), and BSA ( black ). The inset shows SDS-PAGE of purified TodS(23–978) used for SEC-MALS analysis. C , electron microscopic image of gold-labeled TodS proteins. Ni-NTA-gold labeling of C-terminal His 6 -tagged TodS(43–978) proteins was employed to define the molecular arrangement of the TodS dimer. Shown is a negative-stained image showing paired circular gold particles ( yellow arrows ), suggesting a head-to-head dimer of TodS. Scale bar , 50 nm. D , molecular appearance of TodS(23–978) proteins. Electron microscopic images of apo-TodS ( top ), TodS treated with toluene ( middle ), and TodS treated with 1,2,4-TMB ( bottom ) were visualized by negative staining. Ten representative images were selected. Scale bar , 20 nm.
    Tods 23 978 Protein, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation apo tods 23 978 protein
    SEC-MALS and electron microscopic analyses of TodS. A , SEC analysis of purified <t>TodS(23–978).</t> Eluted TodS was compared with the molecular mass standard markers β-amylase (223.8 kDa) and apoferritin (443 kDa). B , SEC-MALS analysis of TodS(23–978). Horizontal lines across the peaks indicate the calculated molecular mass of eluted TodS(23–978) ( red ), β-amylase ( blue ), and BSA ( black ). The inset shows SDS-PAGE of purified TodS(23–978) used for SEC-MALS analysis. C , electron microscopic image of gold-labeled TodS proteins. Ni-NTA-gold labeling of C-terminal His 6 -tagged TodS(43–978) proteins was employed to define the molecular arrangement of the TodS dimer. Shown is a negative-stained image showing paired circular gold particles ( yellow arrows ), suggesting a head-to-head dimer of TodS. Scale bar , 50 nm. D , molecular appearance of TodS(23–978) proteins. Electron microscopic images of apo-TodS ( top ), TodS treated with toluene ( middle ), and TodS treated with 1,2,4-TMB ( bottom ) were visualized by negative staining. Ten representative images were selected. Scale bar , 20 nm.
    Apo Tods 23 978 Protein, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Routledge Ltd isbn 978 0 23 010459 4 caroline sarojini hart
    SEC-MALS and electron microscopic analyses of TodS. A , SEC analysis of purified <t>TodS(23–978).</t> Eluted TodS was compared with the molecular mass standard markers β-amylase (223.8 kDa) and apoferritin (443 kDa). B , SEC-MALS analysis of TodS(23–978). Horizontal lines across the peaks indicate the calculated molecular mass of eluted TodS(23–978) ( red ), β-amylase ( blue ), and BSA ( black ). The inset shows SDS-PAGE of purified TodS(23–978) used for SEC-MALS analysis. C , electron microscopic image of gold-labeled TodS proteins. Ni-NTA-gold labeling of C-terminal His 6 -tagged TodS(43–978) proteins was employed to define the molecular arrangement of the TodS dimer. Shown is a negative-stained image showing paired circular gold particles ( yellow arrows ), suggesting a head-to-head dimer of TodS. Scale bar , 50 nm. D , molecular appearance of TodS(23–978) proteins. Electron microscopic images of apo-TodS ( top ), TodS treated with toluene ( middle ), and TodS treated with 1,2,4-TMB ( bottom ) were visualized by negative staining. Ten representative images were selected. Scale bar , 20 nm.
    Isbn 978 0 23 010459 4 Caroline Sarojini Hart, supplied by Routledge Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Verlag GmbH 2010 isbn 978 3 941468 23 8 jörg walden und heinz grunze 55 seiten
    SEC-MALS and electron microscopic analyses of TodS. A , SEC analysis of purified <t>TodS(23–978).</t> Eluted TodS was compared with the molecular mass standard markers β-amylase (223.8 kDa) and apoferritin (443 kDa). B , SEC-MALS analysis of TodS(23–978). Horizontal lines across the peaks indicate the calculated molecular mass of eluted TodS(23–978) ( red ), β-amylase ( blue ), and BSA ( black ). The inset shows SDS-PAGE of purified TodS(23–978) used for SEC-MALS analysis. C , electron microscopic image of gold-labeled TodS proteins. Ni-NTA-gold labeling of C-terminal His 6 -tagged TodS(43–978) proteins was employed to define the molecular arrangement of the TodS dimer. Shown is a negative-stained image showing paired circular gold particles ( yellow arrows ), suggesting a head-to-head dimer of TodS. Scale bar , 50 nm. D , molecular appearance of TodS(23–978) proteins. Electron microscopic images of apo-TodS ( top ), TodS treated with toluene ( middle ), and TodS treated with 1,2,4-TMB ( bottom ) were visualized by negative staining. Ten representative images were selected. Scale bar , 20 nm.
    2010 Isbn 978 3 941468 23 8 Jörg Walden Und Heinz Grunze 55 Seiten, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    SEC-MALS and electron microscopic analyses of TodS. A , SEC analysis of purified TodS(23–978). Eluted TodS was compared with the molecular mass standard markers β-amylase (223.8 kDa) and apoferritin (443 kDa). B , SEC-MALS analysis of TodS(23–978). Horizontal lines across the peaks indicate the calculated molecular mass of eluted TodS(23–978) ( red ), β-amylase ( blue ), and BSA ( black ). The inset shows SDS-PAGE of purified TodS(23–978) used for SEC-MALS analysis. C , electron microscopic image of gold-labeled TodS proteins. Ni-NTA-gold labeling of C-terminal His 6 -tagged TodS(43–978) proteins was employed to define the molecular arrangement of the TodS dimer. Shown is a negative-stained image showing paired circular gold particles ( yellow arrows ), suggesting a head-to-head dimer of TodS. Scale bar , 50 nm. D , molecular appearance of TodS(23–978) proteins. Electron microscopic images of apo-TodS ( top ), TodS treated with toluene ( middle ), and TodS treated with 1,2,4-TMB ( bottom ) were visualized by negative staining. Ten representative images were selected. Scale bar , 20 nm.

    Journal: The Journal of Biological Chemistry

    Article Title: Molecular Insights into Toluene Sensing in the TodS/TodT Signal Transduction System

    doi: 10.1074/jbc.M116.718841

    Figure Lengend Snippet: SEC-MALS and electron microscopic analyses of TodS. A , SEC analysis of purified TodS(23–978). Eluted TodS was compared with the molecular mass standard markers β-amylase (223.8 kDa) and apoferritin (443 kDa). B , SEC-MALS analysis of TodS(23–978). Horizontal lines across the peaks indicate the calculated molecular mass of eluted TodS(23–978) ( red ), β-amylase ( blue ), and BSA ( black ). The inset shows SDS-PAGE of purified TodS(23–978) used for SEC-MALS analysis. C , electron microscopic image of gold-labeled TodS proteins. Ni-NTA-gold labeling of C-terminal His 6 -tagged TodS(43–978) proteins was employed to define the molecular arrangement of the TodS dimer. Shown is a negative-stained image showing paired circular gold particles ( yellow arrows ), suggesting a head-to-head dimer of TodS. Scale bar , 50 nm. D , molecular appearance of TodS(23–978) proteins. Electron microscopic images of apo-TodS ( top ), TodS treated with toluene ( middle ), and TodS treated with 1,2,4-TMB ( bottom ) were visualized by negative staining. Ten representative images were selected. Scale bar , 20 nm.

    Article Snippet: The N-terminally GST-fused TodS(23–978) protein was expressed in E. coli BL21-CodonPlus (DE3)-RIPL system (Agilent) as described above.

    Techniques: Purification, SDS Page, Labeling, Staining, Negative Staining

    SEC-MALS and electron microscopic analyses of TodS. A , SEC analysis of purified TodS(23–978). Eluted TodS was compared with the molecular mass standard markers β-amylase (223.8 kDa) and apoferritin (443 kDa). B , SEC-MALS analysis of TodS(23–978). Horizontal lines across the peaks indicate the calculated molecular mass of eluted TodS(23–978) ( red ), β-amylase ( blue ), and BSA ( black ). The inset shows SDS-PAGE of purified TodS(23–978) used for SEC-MALS analysis. C , electron microscopic image of gold-labeled TodS proteins. Ni-NTA-gold labeling of C-terminal His 6 -tagged TodS(43–978) proteins was employed to define the molecular arrangement of the TodS dimer. Shown is a negative-stained image showing paired circular gold particles ( yellow arrows ), suggesting a head-to-head dimer of TodS. Scale bar , 50 nm. D , molecular appearance of TodS(23–978) proteins. Electron microscopic images of apo-TodS ( top ), TodS treated with toluene ( middle ), and TodS treated with 1,2,4-TMB ( bottom ) were visualized by negative staining. Ten representative images were selected. Scale bar , 20 nm.

    Journal: The Journal of Biological Chemistry

    Article Title: Molecular Insights into Toluene Sensing in the TodS/TodT Signal Transduction System *

    doi: 10.1074/jbc.M116.718841

    Figure Lengend Snippet: SEC-MALS and electron microscopic analyses of TodS. A , SEC analysis of purified TodS(23–978). Eluted TodS was compared with the molecular mass standard markers β-amylase (223.8 kDa) and apoferritin (443 kDa). B , SEC-MALS analysis of TodS(23–978). Horizontal lines across the peaks indicate the calculated molecular mass of eluted TodS(23–978) ( red ), β-amylase ( blue ), and BSA ( black ). The inset shows SDS-PAGE of purified TodS(23–978) used for SEC-MALS analysis. C , electron microscopic image of gold-labeled TodS proteins. Ni-NTA-gold labeling of C-terminal His 6 -tagged TodS(43–978) proteins was employed to define the molecular arrangement of the TodS dimer. Shown is a negative-stained image showing paired circular gold particles ( yellow arrows ), suggesting a head-to-head dimer of TodS. Scale bar , 50 nm. D , molecular appearance of TodS(23–978) proteins. Electron microscopic images of apo-TodS ( top ), TodS treated with toluene ( middle ), and TodS treated with 1,2,4-TMB ( bottom ) were visualized by negative staining. Ten representative images were selected. Scale bar , 20 nm.

    Article Snippet: The absolute molecular weight of purified apo-TodS(23–978) protein in solution was determined using a Wyatt miniDAWN TREOS, a three-angle static light scattering detector (Wyatt Technology), coupled to an AKTA Purifier FPLC protein purification system equipped with a Superdex G200 column.

    Techniques: Purification, SDS Page, Labeling, Staining, Negative Staining