Journal: PLoS Genetics
Article Title: Anteroposterior Limb Skeletal Patterning Requires the Bifunctional Action of SWI/SNF Chromatin Remodeling Complex in Hedgehog Pathway
doi: 10.1371/journal.pgen.1005915
Figure Lengend Snippet: (A) Expression pattern of Shh in control and Srg3 CKO forelimb buds at indicated stages. Arrowheads and arrow denote the anterior ectopic expression of Shh and its anterior expansion, respectively. (B) Western blot analysis of Gli3FL and Gli3R in lysates from the anterior (Ant) and posterior (Post) halves of E11 control and Srg3 CKO forelimb buds. Values represent the relative levels of Gli3R protein. α-tubulin served as a loading control. (C−E) Spatial distribution of Grem1 , Fgf8 , and Hoxd13 in control and Srg3 CKO forelimb buds. Red arrowheads denote the anterior ectopic expression. In (C), the black bracket indicates the dissociation of Grem1 expression domains and red brackets mark the distance between the distal expression of Grem1 and the AER. Black arrowheads in (D) point to the posterior end of Fgf8 expression. Asterisks in (E) indicate the distalized expression domain of Hoxd13 . Scale bars in (A) and (C−E): 200 μm.
Article Snippet: Fgf4 (Addgene plasmid #22085) [ ] and Fgf8 (Addgene plasmid #22088) [ ] probes were gifts from G. Martin.
Techniques: Expressing, Control, Western Blot