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Agilent technologies 2200 tapestation
2200 Tapestation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2200 tapestation/product/Agilent technologies
Average 99 stars, based on 123 article reviews
Price from $9.99 to $1999.99
2200 tapestation - by Bioz Stars, 2020-10
99/100 stars

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Whole Genome Amplification:

Article Title: Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA
Article Snippet: .. After the amplifications, the yields of WGA reactions were quantified using the PicoGreen® dsDNA Assay Kit (Molecular Probes, Eugene, OR, USA) and by Agilent 2200 TapeStation Instrument with Genomic DNA ScreenTape System (Agilent Technologies, Waldbronn, Germany). .. PicoPlex amplification PicoPlex WGA reactions were carried out using the PicoPLEX WGA Kit (New England Biolabs, Inc., Ipswich, MA, USA).

Picogreen Assay:

Article Title: Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA
Article Snippet: .. After the amplifications, the yields of WGA reactions were quantified using the PicoGreen® dsDNA Assay Kit (Molecular Probes, Eugene, OR, USA) and by Agilent 2200 TapeStation Instrument with Genomic DNA ScreenTape System (Agilent Technologies, Waldbronn, Germany). .. PicoPlex amplification PicoPlex WGA reactions were carried out using the PicoPLEX WGA Kit (New England Biolabs, Inc., Ipswich, MA, USA).

Purification:

Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
Article Snippet: .. The initial evaluation of the quantity and size distribution of the purified gDNA was with the Agilent 2200 TapeStation Nucleic Acid System (G2965AA) controlled by Agilent 2200 TapeStation Software A.01.05, using the Agilent Genomic DNA ScreenTape (5067–5365) and the Agilent Genomic DNA Reagents (5067–5366) with samples drawn from a 96-well plate [ , ] Determination of the quality of the gDNA Fragment gDNA using a Covaris g-TUBE device QC the sizing and adjust the concentration Repair DNA damage and repair ends of fragmented DNA Purify the DNA Blunt-end ligate using blunt adapters Purify template for submission to a sequencer In Fig. , A (Post Shearing Clean-up) and B (10kb Library Prep Runset Dual SPRI) are two of the VWorks protocol graphical user interfaces that help with the NGS Workstation setup and deck layout to optimize the use of reagent volumes. ..

Electrophoresis:

Article Title: Pitfalls of DNA Quantification Using DNA-Binding Fluorescent Dyes and Suggested Solutions
Article Snippet: .. However, the electrophoresis results using an Agilent 2200 TapeStation showed that Trizol-DNAs were weakly stained, appearing as only slightly visible smears ( and ). .. Because SYBR Green I was used for staining DNA for the 2200 TapeStation, these results indicate that the dsDNA concentrations were markedly reduced in Trizol-DNAs.

Article Title: Pitfalls of DNA Quantification Using DNA-Binding Fluorescent Dyes and Suggested Solutions
Article Snippet: .. The size distribution of 100 ng of DNA was also analyzed by electrophoresis using an Agilent 2200 TapeStation ( and ). .. FFPE-DNAs showed a smear, and their DNA integrity numbers (DINs) were 2.2, 3.8 and 2.4 for FFPE-H1, FFPE-H2 and FFPE-H3, respectively, consistent with the qPCR quantities.

Article Title: Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)
Article Snippet: .. In all cases the P . leucotricha DNA was highly fragmented, as indicated by the Agilent 2200 TapeStation® electrophoresis images with fragment sizes between 50 bp– 400 bp. .. DNA quality was measured using NanoDrop 2000™ spectrophotometer (Thermo Fisher Scientific, Wilmington, Delaware, USA) absorbency measurement 260 nm /280 nm ratio; the optimum range indicating high quality DNA is 1.8–1.9 [ ].

Concentration Assay:

Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
Article Snippet: .. The Agilent 2200 TapeStation System with the Genomic DNA assay was used to assess size and concentration of each sample as shown in Fig. , where an electropherogram overlay and virtual gel images are shown for the four model organisms, together with the DIN calculated by the TapeStation software. ..

Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
Article Snippet: .. The initial evaluation of the quantity and size distribution of the purified gDNA was with the Agilent 2200 TapeStation Nucleic Acid System (G2965AA) controlled by Agilent 2200 TapeStation Software A.01.05, using the Agilent Genomic DNA ScreenTape (5067–5365) and the Agilent Genomic DNA Reagents (5067–5366) with samples drawn from a 96-well plate [ , ] Determination of the quality of the gDNA Fragment gDNA using a Covaris g-TUBE device QC the sizing and adjust the concentration Repair DNA damage and repair ends of fragmented DNA Purify the DNA Blunt-end ligate using blunt adapters Purify template for submission to a sequencer In Fig. , A (Post Shearing Clean-up) and B (10kb Library Prep Runset Dual SPRI) are two of the VWorks protocol graphical user interfaces that help with the NGS Workstation setup and deck layout to optimize the use of reagent volumes. ..

other:

Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
Article Snippet: QC of the starting DNA and the required fragment preparation with the Covaris g-TUBE can be done with the Agilent 2200 TapeStation and the gDNA ScreenTape assay directly from the 96 well plates used by the Bravo to prepare the libraries.

Sequencing:

Article Title: Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)
Article Snippet: .. The genomic libraries were quantified by Promega Quantus™ fluorometer and Agilent 2200 TapeStation® and submitted for Illumina® HiSeq 3000 sequencing, except for DneP+ Illumina Nextera XT® and NuGen Ovation® ultralow System V2 libraries which were sequenced using Illumina® MiSeq V3 due to the Illumina® HiSeq 3000 being unavailable. .. Gydle programs were used for sequence read processing ( https://www.gydle.com/ ).

Staining:

Article Title: Pitfalls of DNA Quantification Using DNA-Binding Fluorescent Dyes and Suggested Solutions
Article Snippet: .. However, the electrophoresis results using an Agilent 2200 TapeStation showed that Trizol-DNAs were weakly stained, appearing as only slightly visible smears ( and ). .. Because SYBR Green I was used for staining DNA for the 2200 TapeStation, these results indicate that the dsDNA concentrations were markedly reduced in Trizol-DNAs.

Next-Generation Sequencing:

Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
Article Snippet: .. The initial evaluation of the quantity and size distribution of the purified gDNA was with the Agilent 2200 TapeStation Nucleic Acid System (G2965AA) controlled by Agilent 2200 TapeStation Software A.01.05, using the Agilent Genomic DNA ScreenTape (5067–5365) and the Agilent Genomic DNA Reagents (5067–5366) with samples drawn from a 96-well plate [ , ] Determination of the quality of the gDNA Fragment gDNA using a Covaris g-TUBE device QC the sizing and adjust the concentration Repair DNA damage and repair ends of fragmented DNA Purify the DNA Blunt-end ligate using blunt adapters Purify template for submission to a sequencer In Fig. , A (Post Shearing Clean-up) and B (10kb Library Prep Runset Dual SPRI) are two of the VWorks protocol graphical user interfaces that help with the NGS Workstation setup and deck layout to optimize the use of reagent volumes. ..

Software:

Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
Article Snippet: .. The Agilent 2200 TapeStation System with the Genomic DNA assay was used to assess size and concentration of each sample as shown in Fig. , where an electropherogram overlay and virtual gel images are shown for the four model organisms, together with the DIN calculated by the TapeStation software. ..

Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
Article Snippet: .. The initial evaluation of the quantity and size distribution of the purified gDNA was with the Agilent 2200 TapeStation Nucleic Acid System (G2965AA) controlled by Agilent 2200 TapeStation Software A.01.05, using the Agilent Genomic DNA ScreenTape (5067–5365) and the Agilent Genomic DNA Reagents (5067–5366) with samples drawn from a 96-well plate [ , ] Determination of the quality of the gDNA Fragment gDNA using a Covaris g-TUBE device QC the sizing and adjust the concentration Repair DNA damage and repair ends of fragmented DNA Purify the DNA Blunt-end ligate using blunt adapters Purify template for submission to a sequencer In Fig. , A (Post Shearing Clean-up) and B (10kb Library Prep Runset Dual SPRI) are two of the VWorks protocol graphical user interfaces that help with the NGS Workstation setup and deck layout to optimize the use of reagent volumes. ..

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    Agilent technologies 2200 tapestatio
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    Agilent technologies tapestaion 2200
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