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Agilent technologies 2200 tapestation
2200 Tapestation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2200 tapestation/product/Agilent technologies
Average 99 stars, based on 56 article reviews
Price from $9.99 to $1999.99
2200 tapestation - by Bioz Stars, 2020-02
99/100 stars

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Clone Assay:

Article Title: Multidrug resistant Pseudomonas aeruginosa in Estonian hospitals
Article Snippet: Total bacterial DNA was quantified using a Qubit® 2.0 Fluorometer (Invitrogen, Grand Island, USA) and 2200 TapeStation (Agilent Technologies, Santa Clara, USA). .. Ninety-two ssDNA Nextera XT libraries originating from 92 different clones were pooled and sequenced in one rapid-output run of HiSeq2500 (Illumina, San Diego, USA), with paired-end, 150-bp reads.

Amplification:

Article Title: Arterial endothelial methylome: differential DNA methylation in athero-susceptible disturbed flow regions in vivo
Article Snippet: .. Amplicon size was verified by a 2200 TapeStation (Agilent Technologies, Santa Clara, CA). qPCR primers targeting swine genes are listed in Additional file : Table S3. ..

Article Title: Zika virus evolution and spread in the Americas
Article Snippet: .. After PCR amplification, each amplicon pool was quantified on a 2200 Tapestation (Agilent Technologies) using High Sensitivity D1000 ScreenTape (Agilent Technologies). .. Two microlitres of a 1:10 dilution of the amplicon cDNA was loaded and the concentration of the 350–550-bp fragments was calculated.

Article Title: Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy
Article Snippet: ST counts were used to computationally remove amplification bias post sequencing and relative abundance of each clonotype present in the original sample to be estimated ( ). .. Each pool, which typically contained approximately 90 samples, was profiled on a 2200 TapeStation (Agilent) and concentration determined by real time PCR using a StepOne Real Time Workstation (ABI/Thermo) with a commercial library quantification kit (Kapa Biosystems).

Article Title: The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean). The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean)
Article Snippet: Detailed information on the PCR mixture and amplification conditions is summarized in Appendix . .. The quality and quantity of the purified amplicons were checked using the 2200 TapeStation (Agilent Technologies, Santa Clara, CA, USA).

High Throughput Screening Assay:

Article Title: Identification of Viral Signatures Using High-Throughput Sequencing on Blood of Patients With Kawasaki Disease
Article Snippet: Paragraph title: High-Throughput Sequencing and Sequences Analysis ... Libraries concentrations and size distributions were evaluated with the Q-bit (Life Technologies, Carlsbad,CA, USA) and the 2200 TapeStation (Agilent, SantaClara, CA, USA), respectively.

Synthesized:

Article Title: Arterial endothelial methylome: differential DNA methylation in athero-susceptible disturbed flow regions in vivo
Article Snippet: Real-time RT-PCR cDNA was synthesized by reverse transcribing 0.5-1 μg total RNA with 50 ng random hexamers and SuperScript III reverse transcriptase (Life Technologies). .. Amplicon size was verified by a 2200 TapeStation (Agilent Technologies, Santa Clara, CA). qPCR primers targeting swine genes are listed in Additional file : Table S3.

Quantitative RT-PCR:

Article Title: Arterial endothelial methylome: differential DNA methylation in athero-susceptible disturbed flow regions in vivo
Article Snippet: Paragraph title: Real-time RT-PCR ... Amplicon size was verified by a 2200 TapeStation (Agilent Technologies, Santa Clara, CA). qPCR primers targeting swine genes are listed in Additional file : Table S3.

Electrophoresis:

Article Title: MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence
Article Snippet: Efficient enrichment was verified by Experion Automated Electrophoresis System (Bio-Rad Laboratories) virtual gel analysis and Qubit 3.0 Fluorometer (Thermo Fisher Scientific) analysis. .. Sequencing of mRNAs was performed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and quality control for RNA fragmentation and library construction was assessed by Qubit 3.0 Fluorometer (Thermo Fisher Scientific) and 2200 TapeStation (Agilent) analyses.

Modification:

Article Title: Histone modification profiling in breast cancer cell lines highlights commonalities and differences among subtypes
Article Snippet: ChIP libraries were prepared using a modified Kapa LTP Library Preparation kit (KAPA Biosystems, cat# KK8232) for Illumina Platforms. .. The quality of the final libraries was assessed using a 2200 TapeStation (Agilent Technologies).

Article Title: The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean). The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean)
Article Snippet: A short 312‐bp segment of the rbcL gene was used as the DNA marker and amplified by PCR using an equimolar mix of a modified version of the Diat_rbcL_708F forward and the R3 reverse primers (Rimet, Abarca, et al., ; Vasselon, Rimet, et al., ). .. The quality and quantity of the purified amplicons were checked using the 2200 TapeStation (Agilent Technologies, Santa Clara, CA, USA).

Real-time Polymerase Chain Reaction:

Article Title: Multidrug resistant Pseudomonas aeruginosa in Estonian hospitals
Article Snippet: Total bacterial DNA was quantified using a Qubit® 2.0 Fluorometer (Invitrogen, Grand Island, USA) and 2200 TapeStation (Agilent Technologies, Santa Clara, USA). .. The resulting DNA libraries were validated by qPCR using a Kapa Library Quantification Kit (Kapa Biosystems, Woburn, USA) to optimize cluster generation.

Article Title: The activity of HYDROPEROXIDE LYASE 1 regulates accumulation of galactolipids containing 12-oxo-phytodienoic acid in Arabidopsis
Article Snippet: Paragraph title: Quantitative real-time PCR ... RNA concentration and quality were determined with NanoDrop (Thermo scientific, USA) and 2200 TapeStation (Agilent, USA) respectively.

Article Title: Arterial endothelial methylome: differential DNA methylation in athero-susceptible disturbed flow regions in vivo
Article Snippet: .. Amplicon size was verified by a 2200 TapeStation (Agilent Technologies, Santa Clara, CA). qPCR primers targeting swine genes are listed in Additional file : Table S3. ..

Article Title: Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy
Article Snippet: .. Each pool, which typically contained approximately 90 samples, was profiled on a 2200 TapeStation (Agilent) and concentration determined by real time PCR using a StepOne Real Time Workstation (ABI/Thermo) with a commercial library quantification kit (Kapa Biosystems). .. Paired-end sequencing was performed with a 2 × 150 protocol using a Midoutput 300 sequencing kit on a NextSeq 500 (Illumina).

Flow Cytometry:

Article Title: Histone modification profiling in breast cancer cell lines highlights commonalities and differences among subtypes
Article Snippet: The quality of the final libraries was assessed using a 2200 TapeStation (Agilent Technologies). .. The libraries were quantified using a Kapa Library Quantification Kit (KAPA Biosystems, cat# KK4933) and loaded in a flow cell for cluster generation using the Illumina cBOT (Illumina) at final concentration of 10 pM.

Ligation:

Article Title: Histone modification profiling in breast cancer cell lines highlights commonalities and differences among subtypes
Article Snippet: After adapter ligation, DNA enrichment was performed using Kapa HiFi Hot Start Ready PCR mix, and a cocktail of primers (1 cycle at 98 °C for 45 s; 4 cycles at 98 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 s; and 1 cycle at 72 °C for 1 min), and purified with AmpureXP beads (Beckman Coulter, cat# ). .. The quality of the final libraries was assessed using a 2200 TapeStation (Agilent Technologies).

Genomic Sequencing:

Article Title: Rapid resistome mapping using nanopore sequencing
Article Snippet: DNA QC was performed using Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA) and 2200 TapeStation (Agilent, USA). .. Sequencing library preparation was carried out with Nanopore Genomic Sequencing Kit SQK-MAP006 (Oxford Nanopore, UK) and a PCR-free ‘native barcoding’ kit according to the manufacturer's protocol.

Sequencing:

Article Title: Rapid resistome mapping using nanopore sequencing
Article Snippet: DNA QC was performed using Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA) and 2200 TapeStation (Agilent, USA). .. Sequencing library preparation was carried out with Nanopore Genomic Sequencing Kit SQK-MAP006 (Oxford Nanopore, UK) and a PCR-free ‘native barcoding’ kit according to the manufacturer's protocol.

Article Title: Multidrug resistant Pseudomonas aeruginosa in Estonian hospitals
Article Snippet: Paragraph title: Whole genome sequencing ... Total bacterial DNA was quantified using a Qubit® 2.0 Fluorometer (Invitrogen, Grand Island, USA) and 2200 TapeStation (Agilent Technologies, Santa Clara, USA).

Article Title: MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence
Article Snippet: .. Sequencing of mRNAs was performed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and quality control for RNA fragmentation and library construction was assessed by Qubit 3.0 Fluorometer (Thermo Fisher Scientific) and 2200 TapeStation (Agilent) analyses. .. Template preparation was achieved with an Ion Chef system (Thermo Fisher Scientific) and sample sequencing was done in triplicates with an Ion Proton System (Thermo Fisher Scientific).

Article Title: Identification of Viral Signatures Using High-Throughput Sequencing on Blood of Patients With Kawasaki Disease
Article Snippet: Paragraph title: High-Throughput Sequencing and Sequences Analysis ... Libraries concentrations and size distributions were evaluated with the Q-bit (Life Technologies, Carlsbad,CA, USA) and the 2200 TapeStation (Agilent, SantaClara, CA, USA), respectively.

Article Title: Involvement of tumor necrosis factor alpha in steroid-associated osteonecrosis of the femoral head: friend or foe?
Article Snippet: The quality and integrity of total RNA samples were assessed using a 2100 Bioanalyzer or a 2200 TapeStation (Agilent Technologies) according to the manufacturer’s instructions. .. The preparation of whole transcriptome libraries and deep sequencing were performed by the Annoroad Gene Technology Corporation (Beijing, China).

Article Title: Zika virus evolution and spread in the Americas
Article Snippet: ZIKV amplicons were prepared as described , , similarly to ‘RNA jackhammering’ for preparing low-input viral samples for sequencing , with slight modifications. .. After PCR amplification, each amplicon pool was quantified on a 2200 Tapestation (Agilent Technologies) using High Sensitivity D1000 ScreenTape (Agilent Technologies).

Article Title: Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy
Article Snippet: Paragraph title: TCRβ deep sequencing of tumor and blood ... Each pool, which typically contained approximately 90 samples, was profiled on a 2200 TapeStation (Agilent) and concentration determined by real time PCR using a StepOne Real Time Workstation (ABI/Thermo) with a commercial library quantification kit (Kapa Biosystems).

Article Title: Tenomodulin highly expressing MSCs as a better cell source for tendon injury healing
Article Snippet: The quality and integrity of total RNA samples were assessed using a 2100 Bioanalyzer or a 2200 TapeStation (Agilent Technologies) according to the manufacturer's instructions. .. The preparation of whole transcriptome libraries and deep sequencing were performed by the Annoroad Gene Technology Corporation (Beijing, China).

Article Title: The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification
Article Snippet: Library size was confirmed using 2200 TapeStation and High Sensitivity D1K screen tape (Agilent), and concentration was determined by Library quantification kit (KAPA). .. Filtering of fastq sequence files removed poor quality reads (read length < 18 or > 30% of cycles with Phred score < 23).

Binding Assay:

Article Title: Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy
Article Snippet: Multiplex primers were designed with 5’ overhangs serving as primer binding sites in the 2nd stage PCR. .. Each pool, which typically contained approximately 90 samples, was profiled on a 2200 TapeStation (Agilent) and concentration determined by real time PCR using a StepOne Real Time Workstation (ABI/Thermo) with a commercial library quantification kit (Kapa Biosystems).

Multiplexing:

Article Title: Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy
Article Snippet: To minimize amplification bias due to multiplexing with 20 Vβ forward and 13 Jβ reverse primers, we designed 260 (20V*13J) synthetic TCR templates (STs) with specific barcodes that were added in known concentration to the 1st stage PCR ( ). .. Each pool, which typically contained approximately 90 samples, was profiled on a 2200 TapeStation (Agilent) and concentration determined by real time PCR using a StepOne Real Time Workstation (ABI/Thermo) with a commercial library quantification kit (Kapa Biosystems).

RNA Sequencing Assay:

Article Title: MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence
Article Snippet: .. Sequencing of mRNAs was performed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and quality control for RNA fragmentation and library construction was assessed by Qubit 3.0 Fluorometer (Thermo Fisher Scientific) and 2200 TapeStation (Agilent) analyses. .. Template preparation was achieved with an Ion Chef system (Thermo Fisher Scientific) and sample sequencing was done in triplicates with an Ion Proton System (Thermo Fisher Scientific).

Article Title: Involvement of tumor necrosis factor alpha in steroid-associated osteonecrosis of the femoral head: friend or foe?
Article Snippet: Paragraph title: RNA-seq and data analysis ... The quality and integrity of total RNA samples were assessed using a 2100 Bioanalyzer or a 2200 TapeStation (Agilent Technologies) according to the manufacturer’s instructions.

Article Title: Tenomodulin highly expressing MSCs as a better cell source for tendon injury healing
Article Snippet: Paragraph title: RNA-seq and data analysis ... The quality and integrity of total RNA samples were assessed using a 2100 Bioanalyzer or a 2200 TapeStation (Agilent Technologies) according to the manufacturer's instructions.

Article Title: The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification
Article Snippet: Paragraph title: RNA sequencing ... Library size was confirmed using 2200 TapeStation and High Sensitivity D1K screen tape (Agilent), and concentration was determined by Library quantification kit (KAPA).

Sensitive Assay:

Article Title: Rapid resistome mapping using nanopore sequencing
Article Snippet: .. DNA QC was performed using Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA) and 2200 TapeStation (Agilent, USA). .. Sequencing library preparation was carried out with Nanopore Genomic Sequencing Kit SQK-MAP006 (Oxford Nanopore, UK) and a PCR-free ‘native barcoding’ kit according to the manufacturer's protocol.

Methylation:

Article Title: Prenatal hypoxia-induced epigenomic and transcriptomic reprogramming in rat fetal and adult offspring hearts
Article Snippet: And then, gDNA was denatured with 2 N NaOH at 42 °C for 15 min, treated with sodium bisulfite at 55 °C for 16 h, and purified by EZ DNA Methylation-Gold KitTM (Zymo Research, Tustin, CA, USA) . .. RNA quality was assessed using the 2200 TapeStation (Agilent Technologies, Wilmington, DE USA).

Mutagenesis:

Article Title: MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence
Article Snippet: Sequencing of mRNAs was performed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and quality control for RNA fragmentation and library construction was assessed by Qubit 3.0 Fluorometer (Thermo Fisher Scientific) and 2200 TapeStation (Agilent) analyses. .. To assess differentially expressed genes between Lm WT and ΔmouR mutant strain, sequencing reads were aligned to the reference genome sequence of Listeria monocytogenes EGD-e (RefSeq: GCF_000196035.1, GenBank: GCA_000196035.1 , assembly ASM19603v1 - http://www.ncbi.nlm.nih.gov/assembly/GCF_000196035.1 ) using the aligner TopHat2 ( ).

Isolation:

Article Title: Prenatal hypoxia-induced epigenomic and transcriptomic reprogramming in rat fetal and adult offspring hearts
Article Snippet: Total RNA was isolated from heart tissues using TRIzol RNA Isolation reagent (Life Technologies, Carlsbad, CA, USA). .. RNA quality was assessed using the 2200 TapeStation (Agilent Technologies, Wilmington, DE USA).

Functional Assay:

Article Title: Involvement of tumor necrosis factor alpha in steroid-associated osteonecrosis of the femoral head: friend or foe?
Article Snippet: The quality and integrity of total RNA samples were assessed using a 2100 Bioanalyzer or a 2200 TapeStation (Agilent Technologies) according to the manufacturer’s instructions. .. DAVID bioinformatics tool was also used for functional annotation enrichment and clustering.

Article Title: Tenomodulin highly expressing MSCs as a better cell source for tendon injury healing
Article Snippet: The quality and integrity of total RNA samples were assessed using a 2100 Bioanalyzer or a 2200 TapeStation (Agilent Technologies) according to the manufacturer's instructions. .. DAVID bioinformatics tool was also used for functional annotation enrichment and clustering.

Purification:

Article Title: Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy
Article Snippet: Using 2.0 % of purified PCR product from stage 1 as template, we performed a 2nd stage PCR with universal and indexed Illumina adaptors. .. Each pool, which typically contained approximately 90 samples, was profiled on a 2200 TapeStation (Agilent) and concentration determined by real time PCR using a StepOne Real Time Workstation (ABI/Thermo) with a commercial library quantification kit (Kapa Biosystems).

Article Title: Histone modification profiling in breast cancer cell lines highlights commonalities and differences among subtypes
Article Snippet: After adapter ligation, DNA enrichment was performed using Kapa HiFi Hot Start Ready PCR mix, and a cocktail of primers (1 cycle at 98 °C for 45 s; 4 cycles at 98 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 s; and 1 cycle at 72 °C for 1 min), and purified with AmpureXP beads (Beckman Coulter, cat# ). .. The quality of the final libraries was assessed using a 2200 TapeStation (Agilent Technologies).

Article Title: The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean). The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean)
Article Snippet: .. The quality and quantity of the purified amplicons were checked using the 2200 TapeStation (Agilent Technologies, Santa Clara, CA, USA). .. Following the library preparation method described by Vasselon, Domaizon, et al. , individual A‐X tag adapters (Ion ExpressTM Barcode Adapters, Life Technologies, Carlsbad, CA, USA) were ligated to the amplicons using the NEBNext® Fast DNA Library Prep Set for Ion TorrentTM (BioLabs, Ipswich, MA, USA).

Article Title: Prenatal hypoxia-induced epigenomic and transcriptomic reprogramming in rat fetal and adult offspring hearts
Article Snippet: And then, gDNA was denatured with 2 N NaOH at 42 °C for 15 min, treated with sodium bisulfite at 55 °C for 16 h, and purified by EZ DNA Methylation-Gold KitTM (Zymo Research, Tustin, CA, USA) . .. RNA quality was assessed using the 2200 TapeStation (Agilent Technologies, Wilmington, DE USA).

Polymerase Chain Reaction:

Article Title: Rapid resistome mapping using nanopore sequencing
Article Snippet: DNA QC was performed using Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA) and 2200 TapeStation (Agilent, USA). .. Sequencing library preparation was carried out with Nanopore Genomic Sequencing Kit SQK-MAP006 (Oxford Nanopore, UK) and a PCR-free ‘native barcoding’ kit according to the manufacturer's protocol.

Article Title: Zika virus evolution and spread in the Americas
Article Snippet: .. After PCR amplification, each amplicon pool was quantified on a 2200 Tapestation (Agilent Technologies) using High Sensitivity D1000 ScreenTape (Agilent Technologies). .. Two microlitres of a 1:10 dilution of the amplicon cDNA was loaded and the concentration of the 350–550-bp fragments was calculated.

Article Title: Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy
Article Snippet: Using 2.0 % of purified PCR product from stage 1 as template, we performed a 2nd stage PCR with universal and indexed Illumina adaptors. .. Each pool, which typically contained approximately 90 samples, was profiled on a 2200 TapeStation (Agilent) and concentration determined by real time PCR using a StepOne Real Time Workstation (ABI/Thermo) with a commercial library quantification kit (Kapa Biosystems).

Article Title: Histone modification profiling in breast cancer cell lines highlights commonalities and differences among subtypes
Article Snippet: After adapter ligation, DNA enrichment was performed using Kapa HiFi Hot Start Ready PCR mix, and a cocktail of primers (1 cycle at 98 °C for 45 s; 4 cycles at 98 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 s; and 1 cycle at 72 °C for 1 min), and purified with AmpureXP beads (Beckman Coulter, cat# ). .. The quality of the final libraries was assessed using a 2200 TapeStation (Agilent Technologies).

Article Title: The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean). The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean)
Article Snippet: The three PCR replicates of each DNA sample were pooled and purified using Agencourt AMPure beads (Beckman Coulter, Brea, CA, USA). .. The quality and quantity of the purified amplicons were checked using the 2200 TapeStation (Agilent Technologies, Santa Clara, CA, USA).

Chromatin Immunoprecipitation:

Article Title: Histone modification profiling in breast cancer cell lines highlights commonalities and differences among subtypes
Article Snippet: Paragraph title: Chromatin immunoprecipitation and library preparation ... The quality of the final libraries was assessed using a 2200 TapeStation (Agilent Technologies).

Software:

Article Title: MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence
Article Snippet: Sequencing of mRNAs was performed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and quality control for RNA fragmentation and library construction was assessed by Qubit 3.0 Fluorometer (Thermo Fisher Scientific) and 2200 TapeStation (Agilent) analyses. .. Generation of sequence reads and read trimming and filtering was done with Torrent Suite v4.4 software with a FileExporter v4.4 plugin for generation of FASTQ/BAM files.

SYBR Green Assay:

Article Title: The activity of HYDROPEROXIDE LYASE 1 regulates accumulation of galactolipids containing 12-oxo-phytodienoic acid in Arabidopsis
Article Snippet: RNA concentration and quality were determined with NanoDrop (Thermo scientific, USA) and 2200 TapeStation (Agilent, USA) respectively. .. 1–2 µl of 2× diluted cDNA and primers were mixed with iTaq Fast SYBR Green Supermix with ROX (Bio-Rad, USA) in 15 µl reactions according to the manufacturer’s instructions.

Article Title: Arterial endothelial methylome: differential DNA methylation in athero-susceptible disturbed flow regions in vivo
Article Snippet: Quantitative-PCR (qPCR) was carried out in a Light Cycler 480 with SYBR Green I Master (Roche, Indianapolis, IN) with 2 μl of first strand cDNA and 500nM primers in a final volume of 20 μl. .. Amplicon size was verified by a 2200 TapeStation (Agilent Technologies, Santa Clara, CA). qPCR primers targeting swine genes are listed in Additional file : Table S3.

Multiplex Assay:

Article Title: Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy
Article Snippet: Multiplex primers were designed with 5’ overhangs serving as primer binding sites in the 2nd stage PCR. .. Each pool, which typically contained approximately 90 samples, was profiled on a 2200 TapeStation (Agilent) and concentration determined by real time PCR using a StepOne Real Time Workstation (ABI/Thermo) with a commercial library quantification kit (Kapa Biosystems).

Sample Prep:

Article Title: Multidrug resistant Pseudomonas aeruginosa in Estonian hospitals
Article Snippet: Total bacterial DNA was quantified using a Qubit® 2.0 Fluorometer (Invitrogen, Grand Island, USA) and 2200 TapeStation (Agilent Technologies, Santa Clara, USA). .. Ten nanograms of sample DNA was processed using an Illumina Nextera XT sample preparation kit (Illumina, San Diego, USA).

Article Title: The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification
Article Snippet: Libraries were prepared from 1 μg of total RNA with TruSeq RNA Sample Preparation Kit v2 (Illumina). .. Library size was confirmed using 2200 TapeStation and High Sensitivity D1K screen tape (Agilent), and concentration was determined by Library quantification kit (KAPA).

Sampling:

Article Title: The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean). The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean)
Article Snippet: The quality and quantity of the purified amplicons were checked using the 2200 TapeStation (Agilent Technologies, Santa Clara, CA, USA). .. The sample libraries were pooled into two mixes corresponding to the Mayotte 2014 and 2015 sampling campaigns that contained 49 and 41 samples, respectively.

Concentration Assay:

Article Title: The activity of HYDROPEROXIDE LYASE 1 regulates accumulation of galactolipids containing 12-oxo-phytodienoic acid in Arabidopsis
Article Snippet: .. RNA concentration and quality were determined with NanoDrop (Thermo scientific, USA) and 2200 TapeStation (Agilent, USA) respectively. .. 2 µg of total RNA were subjected to DNase treatment (DNase I, Life technologies, USA) and cDNA synthesis using oligo(dT)18 primers with Superscript III Reverse Transcriptase (Life technologies, USA).

Article Title: Zika virus evolution and spread in the Americas
Article Snippet: After PCR amplification, each amplicon pool was quantified on a 2200 Tapestation (Agilent Technologies) using High Sensitivity D1000 ScreenTape (Agilent Technologies). .. Two microlitres of a 1:10 dilution of the amplicon cDNA was loaded and the concentration of the 350–550-bp fragments was calculated.

Article Title: Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy
Article Snippet: .. Each pool, which typically contained approximately 90 samples, was profiled on a 2200 TapeStation (Agilent) and concentration determined by real time PCR using a StepOne Real Time Workstation (ABI/Thermo) with a commercial library quantification kit (Kapa Biosystems). .. Paired-end sequencing was performed with a 2 × 150 protocol using a Midoutput 300 sequencing kit on a NextSeq 500 (Illumina).

Article Title: Histone modification profiling in breast cancer cell lines highlights commonalities and differences among subtypes
Article Snippet: The quality of the final libraries was assessed using a 2200 TapeStation (Agilent Technologies). .. The libraries were quantified using a Kapa Library Quantification Kit (KAPA Biosystems, cat# KK4933) and loaded in a flow cell for cluster generation using the Illumina cBOT (Illumina) at final concentration of 10 pM.

Article Title: The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification
Article Snippet: .. Library size was confirmed using 2200 TapeStation and High Sensitivity D1K screen tape (Agilent), and concentration was determined by Library quantification kit (KAPA). .. Libraries were multiplexed five per lane and sequenced in a HiSeq2500 (Illumina) to generate 50 million paired end 75 bp reads.

Article Title: The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean). The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean)
Article Snippet: The quality and quantity of the purified amplicons were checked using the 2200 TapeStation (Agilent Technologies, Santa Clara, CA, USA). .. Each mix was adjusted to a final concentration of 100 pm and sequenced independently on two Ion 318TMChip Kit v2 (Life Technologies, Carlsbad, USA) using the PGM Ion Torrent machine.), a consensus taxonomy was provided to each OUT using the classify.otu command with a confidence threshold of 80%.

Marker:

Article Title: The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean). The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean)
Article Snippet: A short 312‐bp segment of the rbcL gene was used as the DNA marker and amplified by PCR using an equimolar mix of a modified version of the Diat_rbcL_708F forward and the R3 reverse primers (Rimet, Abarca, et al., ; Vasselon, Rimet, et al., ). .. The quality and quantity of the purified amplicons were checked using the 2200 TapeStation (Agilent Technologies, Santa Clara, CA, USA).

Lysis:

Article Title: The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean). The impact of OTU sequence similarity threshold on diatom‐based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean)
Article Snippet: This method is preferred for diatom meta‐barcoding (Vasselon, Domaizon, et al., ) because it uses multiple lysis mechanisms (mechanical, enzymatic, and heat‐based) that when combined greatly increase the efficiency of diatom cell lysis and DNA yield. .. The quality and quantity of the purified amplicons were checked using the 2200 TapeStation (Agilent Technologies, Santa Clara, CA, USA).

Nanopore Sequencing:

Article Title: Rapid resistome mapping using nanopore sequencing
Article Snippet: Paragraph title: Nanopore sequencing library preparation ... DNA QC was performed using Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA) and 2200 TapeStation (Agilent, USA).

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    Agilent technologies tapestaion 2200
    Tapestaion 2200, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies agilent 2200 tapestaton
    Agilent 2200 Tapestaton, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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