2100 bioanalyzer  (Agilent technologies)

 
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    Bioanalyzer Instruments
    Description:
    Agilent s refurbished Bioanalyzer Instruments offer reliable performance at a low cost of ownership Agilent Certified Pre Owned Instruments come with one full year of warranty and are guaranteed to perform to the same standards as brand new instruments The Agilent 2100 Bioanalyzer system provides sizing quantitation and quality control of DNA RNA proteins and cells on a single platform providing high quality digital data
    Catalog Number:
    BIOANALYZER-INSTRUMENTS-(REFURBISHED)
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    Products Agilent Certified Pre Owned Instruments Agilent Certified Spectroscopy And Other Instruments Bioanalyzer Instruments Refurbished
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    Structured Review

    Agilent technologies 2100 bioanalyzer
    Bioanalyser images demonstrating quality of two FFPE RRBS libraries. Each of the RRBS libraries (FFPE1 and FFPE2) was run on an Agilent <t>2100</t> <t>Bioanalyzer</t> using the high sensitivity DNA kit. The electropherogram displays a plot of fragment size (bp) versus fluorescence intensity. Peaks at 35 bp and 10,380 bp represent lower and upper markers. The 160–340 bp peaks represent the RRBS library
    Agilent s refurbished Bioanalyzer Instruments offer reliable performance at a low cost of ownership Agilent Certified Pre Owned Instruments come with one full year of warranty and are guaranteed to perform to the same standards as brand new instruments The Agilent 2100 Bioanalyzer system provides sizing quantitation and quality control of DNA RNA proteins and cells on a single platform providing high quality digital data
    https://www.bioz.com/result/2100 bioanalyzer/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2100 bioanalyzer - by Bioz Stars, 2021-05
    86/100 stars

    Images

    1) Product Images from "A streamlined method for analysing genome-wide DNA methylation patterns from low amounts of FFPE DNA"

    Article Title: A streamlined method for analysing genome-wide DNA methylation patterns from low amounts of FFPE DNA

    Journal: BMC Medical Genomics

    doi: 10.1186/s12920-017-0290-1

    Bioanalyser images demonstrating quality of two FFPE RRBS libraries. Each of the RRBS libraries (FFPE1 and FFPE2) was run on an Agilent 2100 Bioanalyzer using the high sensitivity DNA kit. The electropherogram displays a plot of fragment size (bp) versus fluorescence intensity. Peaks at 35 bp and 10,380 bp represent lower and upper markers. The 160–340 bp peaks represent the RRBS library
    Figure Legend Snippet: Bioanalyser images demonstrating quality of two FFPE RRBS libraries. Each of the RRBS libraries (FFPE1 and FFPE2) was run on an Agilent 2100 Bioanalyzer using the high sensitivity DNA kit. The electropherogram displays a plot of fragment size (bp) versus fluorescence intensity. Peaks at 35 bp and 10,380 bp represent lower and upper markers. The 160–340 bp peaks represent the RRBS library

    Techniques Used: Formalin-fixed Paraffin-Embedded, Fluorescence

    Related Articles

    Spectrophotometry:

    Article Title: Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-seq)
    Article Snippet: 5 M NaCl Proteinase K (Promega) 1:1 Phenol:Chloroform 100% Ethanol TE buffer: 10 mM Tris pH 8, 10 mM EDTA pH 8 Nanodrop spectrophotometer .. Covaris sonicator (S220, formerly S2) Covaris MicroTUBE AFA Pre-slit Snap-Cap 6x16mm Nanodrop Spectrophotometer DNA High Sensitivity Kit (Agilent) for use with Agilent 2100 Bioanalyzer .. EZ DNA Methylation Kit #D5001 (Zymo Research)

    Chromatin Immunoprecipitation:

    Article Title: HOPE-BAL
    Article Snippet: .. RNA quality was analyzed on an Agilent Bioanalyzer with RNA 6000 Nano Chip assay (Agilent, Böblingen, Germany) according to the manufacturer’s instructions. .. For cDNA synthesis, 1 µg of total RNA was subjected to DNAse I treatment with 1 µl of 10× DNAse I reaction buffer (Invitrogen, Karlsruhe, Germany) for 15 min at room temperature.

    Agarose Gel Electrophoresis:

    Article Title: Expression microarray reproducibility is improved by optimising purification steps in RNA amplification and labelling
    Article Snippet: Effect of genomic DNA carry over on RNA amplification To evaluate the optimal method for removal of genomic DNA contamination from RNA samples, total RNA from each of the five cell lines was treated using one of four methods: Qiagen RNeasy minikit columns (with on-column DNase digestion); DNase treatment followed by 2.5 M LiCl precipitation, and DNase treatment (using two different RNA concentrations, D5 and D20) followed by PCI extraction. .. The efficiency of each method was first assessed by agarose gel and Agilent Bioanalyzer. .. Agarose gel was sufficient to demonstrate that specimens purified by the LiCl method had a high level of genomic DNA contamination (Figure ).

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    Agilent technologies agilent 2100 bioanalyzer
    Analysis of accumulation of transcripts and proteins of representative genes associated with chloroplast biogenesis in wild-type (WT) and wsl5 seedlings. (A, C) Western blot analysis of chloroplast proteins and RCA in WT and wsl5 seedlings at the third-leaf stage at C20 (A) and C30 (C). Hsp90 was used as an internal control. (B, D) qRT-PCR analysis of relative expression levels of plastid-encoded genes in the WT and wsl5 at the third-leaf stage under (B) C20 or (D) C30. Error bars represent the SD from three independent experiments. (E, F) rRNA analysis using an <t>Agilent</t> 2100 <t>bioanalyzer.</t> RNA was isolated from 10-day-old WT and wsl5 seedlings grown at C30 and C20 (Student’s t -test, ** P
    Agilent 2100 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent 2100 bioanalyzer/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    agilent 2100 bioanalyzer - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    99
    Agilent technologies 2100 agilent bioanalyzer
    Absence of gnd gene in S. bongori strains . The gnd gene was PCR-amplified as described in the Materials and Methods from eight different strains of S. bongori . PCR products were resolved using the <t>Agilent</t> 7500 DNA kit and the Agilent <t>2100</t> <t>Bioanalyzer.</t> Negative Control 1 corresponds to PCR reagents mix + water as a template; and Negative Control 2 corresponds to DNA from a known gnd negative bacterial strain using our PCR conditions.
    2100 Agilent Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2100 agilent bioanalyzer/product/Agilent technologies
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2100 agilent bioanalyzer - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    Analysis of accumulation of transcripts and proteins of representative genes associated with chloroplast biogenesis in wild-type (WT) and wsl5 seedlings. (A, C) Western blot analysis of chloroplast proteins and RCA in WT and wsl5 seedlings at the third-leaf stage at C20 (A) and C30 (C). Hsp90 was used as an internal control. (B, D) qRT-PCR analysis of relative expression levels of plastid-encoded genes in the WT and wsl5 at the third-leaf stage under (B) C20 or (D) C30. Error bars represent the SD from three independent experiments. (E, F) rRNA analysis using an Agilent 2100 bioanalyzer. RNA was isolated from 10-day-old WT and wsl5 seedlings grown at C30 and C20 (Student’s t -test, ** P

    Journal: Journal of Experimental Botany

    Article Title: WSL5, a pentatricopeptide repeat protein, is essential for chloroplast biogenesis in rice under cold stress

    doi: 10.1093/jxb/ery214

    Figure Lengend Snippet: Analysis of accumulation of transcripts and proteins of representative genes associated with chloroplast biogenesis in wild-type (WT) and wsl5 seedlings. (A, C) Western blot analysis of chloroplast proteins and RCA in WT and wsl5 seedlings at the third-leaf stage at C20 (A) and C30 (C). Hsp90 was used as an internal control. (B, D) qRT-PCR analysis of relative expression levels of plastid-encoded genes in the WT and wsl5 at the third-leaf stage under (B) C20 or (D) C30. Error bars represent the SD from three independent experiments. (E, F) rRNA analysis using an Agilent 2100 bioanalyzer. RNA was isolated from 10-day-old WT and wsl5 seedlings grown at C30 and C20 (Student’s t -test, ** P

    Article Snippet: We analyzed the composition and content of rRNAs using an Agilent 2100 bioanalyzer under C20 and C30 conditions.

    Techniques: Western Blot, Quantitative RT-PCR, Expressing, Isolation

    Examples of Agilent 2100 Bioanalyzer spectra of total RNA showing improvement with Options 1 and 2 compared to Option 3. Each graph shows the intensity of the peaks of the ribosomal RNA subunits: nuclear large-28S, small-18S, cytoplasmic, mitochondrial, and chloroplastic (smaller subunits). The electrophoretic gel for each sample is shown to the right indicating the subunit bands or degradation (i.e., smear). nt = number of estimated nucleotides based on ladder; FU = fluorescence unit (i.e., intensity of peak). (A) Degraded ribosomal RNA subunits of Canella winterana (L.) Gaertn. extracted with Option 3 that resulted in an estimation of 27 μg of RNA, but the subunits are degraded. (B) A second example using Option 3, Muntingia calabura L., also shows an inflated quantity reading with degraded subunits. (C) Canella winterana , extracted with Option 1, indicating peaks for intact ribosomal RNA subunits. (D) Muntingia calabura extracted with Option 2.

    Journal: Applications in Plant Sciences

    Article Title: Modified CTAB and TRIzol protocols improve RNA extraction from chemically complex Embryophyta 1

    doi: 10.3732/apps.1400105

    Figure Lengend Snippet: Examples of Agilent 2100 Bioanalyzer spectra of total RNA showing improvement with Options 1 and 2 compared to Option 3. Each graph shows the intensity of the peaks of the ribosomal RNA subunits: nuclear large-28S, small-18S, cytoplasmic, mitochondrial, and chloroplastic (smaller subunits). The electrophoretic gel for each sample is shown to the right indicating the subunit bands or degradation (i.e., smear). nt = number of estimated nucleotides based on ladder; FU = fluorescence unit (i.e., intensity of peak). (A) Degraded ribosomal RNA subunits of Canella winterana (L.) Gaertn. extracted with Option 3 that resulted in an estimation of 27 μg of RNA, but the subunits are degraded. (B) A second example using Option 3, Muntingia calabura L., also shows an inflated quantity reading with degraded subunits. (C) Canella winterana , extracted with Option 1, indicating peaks for intact ribosomal RNA subunits. (D) Muntingia calabura extracted with Option 2.

    Article Snippet: The Agilent 2100 Bioanalyzer uses 2–5-μL aliquots of the samples; consult with your core facility on the desired volume.

    Techniques: Fluorescence

    Absence of gnd gene in S. bongori strains . The gnd gene was PCR-amplified as described in the Materials and Methods from eight different strains of S. bongori . PCR products were resolved using the Agilent 7500 DNA kit and the Agilent 2100 Bioanalyzer. Negative Control 1 corresponds to PCR reagents mix + water as a template; and Negative Control 2 corresponds to DNA from a known gnd negative bacterial strain using our PCR conditions.

    Journal: Frontiers in Microbiology

    Article Title: Differentiation of Salmonella strains from the SARA, SARB and SARC reference collections by using three genes PCR-RFLP and the 2100 Agilent Bioanalyzer

    doi: 10.3389/fmicb.2014.00417

    Figure Lengend Snippet: Absence of gnd gene in S. bongori strains . The gnd gene was PCR-amplified as described in the Materials and Methods from eight different strains of S. bongori . PCR products were resolved using the Agilent 7500 DNA kit and the Agilent 2100 Bioanalyzer. Negative Control 1 corresponds to PCR reagents mix + water as a template; and Negative Control 2 corresponds to DNA from a known gnd negative bacterial strain using our PCR conditions.

    Article Snippet: Previous studies have demonstrated improved accuracy and reproducibility of RFLP using the 2100 Agilent Bioanalyzer for the sizing of the DNA fragments (Panaro et al., ; Nachamkin et al., ; Lu et al., ; Hathaway et al., ).

    Techniques: Polymerase Chain Reaction, Amplification, Negative Control

    Amplification of HSV-1 (lane 2), HSV-2 (lane 3), CMV (lane 4), West Nile virus (lane 5), and poliovirus 1 (lane 6). Lane 1, molecular weight markers. The PCR products were separated by using an Agilent 2100 bioanalyzer.

    Journal:

    Article Title: DNA Probe Array for the Simultaneous Identification of Herpesviruses, Enteroviruses, and Flaviviruses

    doi: 10.1128/JCM.43.8.3779-3787.2005

    Figure Lengend Snippet: Amplification of HSV-1 (lane 2), HSV-2 (lane 3), CMV (lane 4), West Nile virus (lane 5), and poliovirus 1 (lane 6). Lane 1, molecular weight markers. The PCR products were separated by using an Agilent 2100 bioanalyzer.

    Article Snippet: Amplicons were quantified with an Agilent 2100 bioanalyzer and a DNA 1000 LabProbe array (Agilent Technologies, Massy, France), according to the manufacturer's instructions.

    Techniques: Amplification, Molecular Weight, Polymerase Chain Reaction