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Agilent technologies 2100 bioanalyzer system
Analysis of tear proteins obtained by running aliquots from the same samples in parallel. Tear samples (one to eight) were runned through the <t>2100</t> <t>Bioanalyzer</t> (upper part of the figure, Protein 230 kit, gel-like view), and the 1D SDS–PAGE (lower part of the figure). The corresponding molecular weight in kDa is reported on the left of the ladder L : and of the prestained protein standard S : The same profiles were obtained for each sample in the two analytical methods.
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1) Product Images from "A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins"

Article Title: A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins

Journal: Molecular Vision

doi:

Analysis of tear proteins obtained by running aliquots from the same samples in parallel. Tear samples (one to eight) were runned through the 2100 Bioanalyzer (upper part of the figure, Protein 230 kit, gel-like view), and the 1D SDS–PAGE (lower part of the figure). The corresponding molecular weight in kDa is reported on the left of the ladder L : and of the prestained protein standard S : The same profiles were obtained for each sample in the two analytical methods.
Figure Legend Snippet: Analysis of tear proteins obtained by running aliquots from the same samples in parallel. Tear samples (one to eight) were runned through the 2100 Bioanalyzer (upper part of the figure, Protein 230 kit, gel-like view), and the 1D SDS–PAGE (lower part of the figure). The corresponding molecular weight in kDa is reported on the left of the ladder L : and of the prestained protein standard S : The same profiles were obtained for each sample in the two analytical methods.

Techniques Used: SDS Page, Molecular Weight

Inter-practitioner (Techn1 and Techn2) measurement variability (reproducibility) for the Agilent 2100 Bioanalyzer is shown. A : The tear lysozyme measurements (mg/ml) performed by Techn1 (y-axis) and by Techn 2 (x-axis) on the same tear samples are graphed. B : The Bland–Altman plot for various tear samples analyzed for lysozyme content, performed by two different laboratory technicians (Techn 1 and Techn 2), is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained by Techn 1 and Techn 2. The y-axis indicates the difference in measurement between the two technicians. The mean difference of the technicians is 0.01, with an upper specification limit of 0.23 and a lower specification limit of 0.20. C : The concordance correlation coefficients calculated for the two technicians are shown; all values demonstrated the high reproducibility of the Bioanalyzer method.
Figure Legend Snippet: Inter-practitioner (Techn1 and Techn2) measurement variability (reproducibility) for the Agilent 2100 Bioanalyzer is shown. A : The tear lysozyme measurements (mg/ml) performed by Techn1 (y-axis) and by Techn 2 (x-axis) on the same tear samples are graphed. B : The Bland–Altman plot for various tear samples analyzed for lysozyme content, performed by two different laboratory technicians (Techn 1 and Techn 2), is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained by Techn 1 and Techn 2. The y-axis indicates the difference in measurement between the two technicians. The mean difference of the technicians is 0.01, with an upper specification limit of 0.23 and a lower specification limit of 0.20. C : The concordance correlation coefficients calculated for the two technicians are shown; all values demonstrated the high reproducibility of the Bioanalyzer method.

Techniques Used:

Degree of agreement between measurements conducted on replicate specimens. The same tear samples were runned with two methods: monodimensional sodium dodecyl sulfate-polyacrylamide lectrophoresis (1D SDS–PAGE) electrophoresis and the 2100 Agilent Bioanalyzer (Protein 230 kit). A : The measurements obtained with 1D-SDS–PAGE electrophoresis (y-axis) and the 2100 Agilent Bioanalyzer (x-axis) are graphed. B : The Bland–Altman plot for various samples of tears analyzed for lysozyme content, performed with the two methods, is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained with 1D SDS–PAGE electrophoresis and the 2100 Agilent Bioanalyzer. The y-axis indicates the difference in measurement between the two methods. The mean difference is 0.08, with an upper specification limit of 0.28 and a lower specification limit of 0.43. C : The concordance correlation coefficients calculated for the two methods are shown; all values demonstrated high correlation between the two methods.
Figure Legend Snippet: Degree of agreement between measurements conducted on replicate specimens. The same tear samples were runned with two methods: monodimensional sodium dodecyl sulfate-polyacrylamide lectrophoresis (1D SDS–PAGE) electrophoresis and the 2100 Agilent Bioanalyzer (Protein 230 kit). A : The measurements obtained with 1D-SDS–PAGE electrophoresis (y-axis) and the 2100 Agilent Bioanalyzer (x-axis) are graphed. B : The Bland–Altman plot for various samples of tears analyzed for lysozyme content, performed with the two methods, is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained with 1D SDS–PAGE electrophoresis and the 2100 Agilent Bioanalyzer. The y-axis indicates the difference in measurement between the two methods. The mean difference is 0.08, with an upper specification limit of 0.28 and a lower specification limit of 0.43. C : The concordance correlation coefficients calculated for the two methods are shown; all values demonstrated high correlation between the two methods.

Techniques Used: SDS Page, Electrophoresis

Inter-session (session 1 and session 2) measurement variability (repeatability) for the Agilent 2100 Bioanalyzer is shown. A : The tear lysozyme measurements (mg/ml) performed during session 1 (y-axis) and session 2 (x-axis) on the same tear samples are graphed. B : The Bland–Altman plot for various tear samples analyzed for lysozyme content, performed in two sessions, is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained in session 1 and session 2. The y-axis indicates the difference in measurement between the two sessions. The mean difference of both sessions is 0.04, with an upper specification limit of 0.24 and a lower specification limit of 0.16. C : The concordance correlation coefficients calculated for the two sessions are shown; all values demonstrated the high repeatability of the Bioanalyzer method.
Figure Legend Snippet: Inter-session (session 1 and session 2) measurement variability (repeatability) for the Agilent 2100 Bioanalyzer is shown. A : The tear lysozyme measurements (mg/ml) performed during session 1 (y-axis) and session 2 (x-axis) on the same tear samples are graphed. B : The Bland–Altman plot for various tear samples analyzed for lysozyme content, performed in two sessions, is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained in session 1 and session 2. The y-axis indicates the difference in measurement between the two sessions. The mean difference of both sessions is 0.04, with an upper specification limit of 0.24 and a lower specification limit of 0.16. C : The concordance correlation coefficients calculated for the two sessions are shown; all values demonstrated the high repeatability of the Bioanalyzer method.

Techniques Used:

2) Product Images from "A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins"

Article Title: A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins

Journal: Molecular Vision

doi:

Analysis of tear proteins obtained by running aliquots from the same samples in parallel. Tear samples (one to eight) were runned through the 2100 Bioanalyzer (upper part of the figure, Protein 230 kit, gel-like view), and the 1D SDS–PAGE (lower part of the figure). The corresponding molecular weight in kDa is reported on the left of the ladder L : and of the prestained protein standard S : The same profiles were obtained for each sample in the two analytical methods.
Figure Legend Snippet: Analysis of tear proteins obtained by running aliquots from the same samples in parallel. Tear samples (one to eight) were runned through the 2100 Bioanalyzer (upper part of the figure, Protein 230 kit, gel-like view), and the 1D SDS–PAGE (lower part of the figure). The corresponding molecular weight in kDa is reported on the left of the ladder L : and of the prestained protein standard S : The same profiles were obtained for each sample in the two analytical methods.

Techniques Used: SDS Page, Molecular Weight

Inter-practitioner (Techn1 and Techn2) measurement variability (reproducibility) for the Agilent 2100 Bioanalyzer is shown. A : The tear lysozyme measurements (mg/ml) performed by Techn1 (y-axis) and by Techn 2 (x-axis) on the same tear samples are graphed. B : The Bland–Altman plot for various tear samples analyzed for lysozyme content, performed by two different laboratory technicians (Techn 1 and Techn 2), is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained by Techn 1 and Techn 2. The y-axis indicates the difference in measurement between the two technicians. The mean difference of the technicians is 0.01, with an upper specification limit of 0.23 and a lower specification limit of 0.20. C : The concordance correlation coefficients calculated for the two technicians are shown; all values demonstrated the high reproducibility of the Bioanalyzer method.
Figure Legend Snippet: Inter-practitioner (Techn1 and Techn2) measurement variability (reproducibility) for the Agilent 2100 Bioanalyzer is shown. A : The tear lysozyme measurements (mg/ml) performed by Techn1 (y-axis) and by Techn 2 (x-axis) on the same tear samples are graphed. B : The Bland–Altman plot for various tear samples analyzed for lysozyme content, performed by two different laboratory technicians (Techn 1 and Techn 2), is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained by Techn 1 and Techn 2. The y-axis indicates the difference in measurement between the two technicians. The mean difference of the technicians is 0.01, with an upper specification limit of 0.23 and a lower specification limit of 0.20. C : The concordance correlation coefficients calculated for the two technicians are shown; all values demonstrated the high reproducibility of the Bioanalyzer method.

Techniques Used:

Degree of agreement between measurements conducted on replicate specimens. The same tear samples were runned with two methods: monodimensional sodium dodecyl sulfate-polyacrylamide lectrophoresis (1D SDS–PAGE) electrophoresis and the 2100 Agilent Bioanalyzer (Protein 230 kit). A : The measurements obtained with 1D-SDS–PAGE electrophoresis (y-axis) and the 2100 Agilent Bioanalyzer (x-axis) are graphed. B : The Bland–Altman plot for various samples of tears analyzed for lysozyme content, performed with the two methods, is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained with 1D SDS–PAGE electrophoresis and the 2100 Agilent Bioanalyzer. The y-axis indicates the difference in measurement between the two methods. The mean difference is 0.08, with an upper specification limit of 0.28 and a lower specification limit of 0.43. C : The concordance correlation coefficients calculated for the two methods are shown; all values demonstrated high correlation between the two methods.
Figure Legend Snippet: Degree of agreement between measurements conducted on replicate specimens. The same tear samples were runned with two methods: monodimensional sodium dodecyl sulfate-polyacrylamide lectrophoresis (1D SDS–PAGE) electrophoresis and the 2100 Agilent Bioanalyzer (Protein 230 kit). A : The measurements obtained with 1D-SDS–PAGE electrophoresis (y-axis) and the 2100 Agilent Bioanalyzer (x-axis) are graphed. B : The Bland–Altman plot for various samples of tears analyzed for lysozyme content, performed with the two methods, is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained with 1D SDS–PAGE electrophoresis and the 2100 Agilent Bioanalyzer. The y-axis indicates the difference in measurement between the two methods. The mean difference is 0.08, with an upper specification limit of 0.28 and a lower specification limit of 0.43. C : The concordance correlation coefficients calculated for the two methods are shown; all values demonstrated high correlation between the two methods.

Techniques Used: SDS Page, Electrophoresis

Inter-session (session 1 and session 2) measurement variability (repeatability) for the Agilent 2100 Bioanalyzer is shown. A : The tear lysozyme measurements (mg/ml) performed during session 1 (y-axis) and session 2 (x-axis) on the same tear samples are graphed. B : The Bland–Altman plot for various tear samples analyzed for lysozyme content, performed in two sessions, is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained in session 1 and session 2. The y-axis indicates the difference in measurement between the two sessions. The mean difference of both sessions is 0.04, with an upper specification limit of 0.24 and a lower specification limit of 0.16. C : The concordance correlation coefficients calculated for the two sessions are shown; all values demonstrated the high repeatability of the Bioanalyzer method.
Figure Legend Snippet: Inter-session (session 1 and session 2) measurement variability (repeatability) for the Agilent 2100 Bioanalyzer is shown. A : The tear lysozyme measurements (mg/ml) performed during session 1 (y-axis) and session 2 (x-axis) on the same tear samples are graphed. B : The Bland–Altman plot for various tear samples analyzed for lysozyme content, performed in two sessions, is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained in session 1 and session 2. The y-axis indicates the difference in measurement between the two sessions. The mean difference of both sessions is 0.04, with an upper specification limit of 0.24 and a lower specification limit of 0.16. C : The concordance correlation coefficients calculated for the two sessions are shown; all values demonstrated the high repeatability of the Bioanalyzer method.

Techniques Used:

3) Product Images from "Analysis of the complementarity determining regions β-chain genomic rearrangement using high-throughput sequencing in periphery cytotoxic T lymphocytes of patients with chronic hepatitis B"

Article Title: Analysis of the complementarity determining regions β-chain genomic rearrangement using high-throughput sequencing in periphery cytotoxic T lymphocytes of patients with chronic hepatitis B

Journal: Molecular Medicine Reports

doi: 10.3892/mmr.2016.5329

Mimic diagram of size distribution of polymerase chain reaction products with Agilent 2100 Bioanalyzer system analysis.
Figure Legend Snippet: Mimic diagram of size distribution of polymerase chain reaction products with Agilent 2100 Bioanalyzer system analysis.

Techniques Used: Polymerase Chain Reaction

Agilent 2100 Bioanalyzer system analyzed data from samples of one patient (prior and subsequent to treatment). (A) Blank control. Size distribution was measured and polymerase chain reaction product quality was tested with Agilent 2100 Bioanalyzer analysis (B) prior to and (C) following treatment.
Figure Legend Snippet: Agilent 2100 Bioanalyzer system analyzed data from samples of one patient (prior and subsequent to treatment). (A) Blank control. Size distribution was measured and polymerase chain reaction product quality was tested with Agilent 2100 Bioanalyzer analysis (B) prior to and (C) following treatment.

Techniques Used: Polymerase Chain Reaction

4) Product Images from "Analysis of the complementarity determining regions β-chain genomic rearrangement using high-throughput sequencing in periphery cytotoxic T lymphocytes of patients with chronic hepatitis B"

Article Title: Analysis of the complementarity determining regions β-chain genomic rearrangement using high-throughput sequencing in periphery cytotoxic T lymphocytes of patients with chronic hepatitis B

Journal: Molecular Medicine Reports

doi: 10.3892/mmr.2016.5329

Mimic diagram of size distribution of polymerase chain reaction products with Agilent 2100 Bioanalyzer system analysis.
Figure Legend Snippet: Mimic diagram of size distribution of polymerase chain reaction products with Agilent 2100 Bioanalyzer system analysis.

Techniques Used: Polymerase Chain Reaction

Agilent 2100 Bioanalyzer system analyzed data from samples of one patient (prior and subsequent to treatment). (A) Blank control. Size distribution was measured and polymerase chain reaction product quality was tested with Agilent 2100 Bioanalyzer analysis (B) prior to and (C) following treatment.
Figure Legend Snippet: Agilent 2100 Bioanalyzer system analyzed data from samples of one patient (prior and subsequent to treatment). (A) Blank control. Size distribution was measured and polymerase chain reaction product quality was tested with Agilent 2100 Bioanalyzer analysis (B) prior to and (C) following treatment.

Techniques Used: Polymerase Chain Reaction

5) Product Images from "Diagnostic performance of a tear protein panel in early dry eye"

Article Title: Diagnostic performance of a tear protein panel in early dry eye

Journal: Molecular Vision

doi:

Data from 2100 Bioanalyzer analysis.Upper left: Electropherograms from a representative normal subject (blue line) and a dry eye (DE) patient (red line) are here aligned and overlapped. Recognized peaks of interest are numbered 1 through 11. Upper right: The virtual gel images related to both samples are here compared showing different intensity of corresponding bands between normal subject and DE patient. Bands are here also numbered 1 through 11. Table at the bottom summarizes for each peak the following parameters: recognized molecular weight in kDa, name of the protein assigned on the basis of the validation process [ 13 ], concentration of each protein expressed in ng/microliter, percentage of each protein versus total protein content for both (N) normal subject and (DE) patient. The last line of this table reports total protein concentration expressed in ng/microliter
Figure Legend Snippet: Data from 2100 Bioanalyzer analysis.Upper left: Electropherograms from a representative normal subject (blue line) and a dry eye (DE) patient (red line) are here aligned and overlapped. Recognized peaks of interest are numbered 1 through 11. Upper right: The virtual gel images related to both samples are here compared showing different intensity of corresponding bands between normal subject and DE patient. Bands are here also numbered 1 through 11. Table at the bottom summarizes for each peak the following parameters: recognized molecular weight in kDa, name of the protein assigned on the basis of the validation process [ 13 ], concentration of each protein expressed in ng/microliter, percentage of each protein versus total protein content for both (N) normal subject and (DE) patient. The last line of this table reports total protein concentration expressed in ng/microliter

Techniques Used: Molecular Weight, Concentration Assay, Protein Concentration

6) Product Images from "Frequencies of genetic polymorphisms related to triptans metabolism in chronic migraine"

Article Title: Frequencies of genetic polymorphisms related to triptans metabolism in chronic migraine

Journal: The Journal of Headache and Pain

doi: 10.1007/s10194-010-0202-7

Comparison between classic agarose gel electrophoresis and chip technology for resolution and accuracy. The same samples were analyzed by electrophoresis onto 3% agarose gel ( a ) and Bioanalyzer 2100 ( b ). The molecular weight of the upper band in the S1 line of the agarose gel is not unambiguously assignable. b Gel-like image and electropherograms of samples S1 and S2 obtained by Bioanalyzer 2100 analysis. The exact length of the longer fragment is easily determined ( Mk molecular weight marker)
Figure Legend Snippet: Comparison between classic agarose gel electrophoresis and chip technology for resolution and accuracy. The same samples were analyzed by electrophoresis onto 3% agarose gel ( a ) and Bioanalyzer 2100 ( b ). The molecular weight of the upper band in the S1 line of the agarose gel is not unambiguously assignable. b Gel-like image and electropherograms of samples S1 and S2 obtained by Bioanalyzer 2100 analysis. The exact length of the longer fragment is easily determined ( Mk molecular weight marker)

Techniques Used: Agarose Gel Electrophoresis, Chromatin Immunoprecipitation, Electrophoresis, Molecular Weight, Marker

7) Product Images from "Targeted next generation sequencing for molecular diagnosis of Usher syndrome"

Article Title: Targeted next generation sequencing for molecular diagnosis of Usher syndrome

Journal: Orphanet Journal of Rare Diseases

doi: 10.1186/s13023-014-0168-7

Images obtained in the validation and quantification of the enriched target DNA with the 2100 Bioanalyzer system. A) Typical image obtained in most patients. B) Atypical image obtained in the patient RP-531, in whom CNV analysis could not be performed.
Figure Legend Snippet: Images obtained in the validation and quantification of the enriched target DNA with the 2100 Bioanalyzer system. A) Typical image obtained in most patients. B) Atypical image obtained in the patient RP-531, in whom CNV analysis could not be performed.

Techniques Used:

8) Product Images from "Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read ( > 11 kb), single molecule, real-time sequencing"

Article Title: Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read ( > 11 kb), single molecule, real-time sequencing

Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

doi: 10.1093/dnares/dsw022

Comparison of SMRTbell library preparation efficiency from P. falciparum genomic DNA purified using three different methods. (A) High molecular weight P. falciparum genomic DNA prepared from an asynchronous culture using the Genomic tip kit was purified by three different methods: (i) AMPure PB magnetic bead-based clean up (Lanes 1 2), (ii) electrophoretic DNA extraction using the Aurora System (Lanes 3 4) or (iii) phenol-chloroform extraction (Lanes 5 6), and sheared as described in Materials and Methods. Quality and size distribution of the sheared DNA (140 ng) was assessed using field-inversion gel electrophoresis (Pippin Pulse System, Sage Science). Size markers included CHEF 8-48 kb DNA Size Standard (Bio-Rad) and 2.5 kb Molecular Ruler (Bio-Rad). (B) SMRTbell libraries prepared using the indicated amount of purified genomic DNA was subjected to size selection on the BluePippin System using a 15 kb cut-off. The DNA yield and % recovery of various steps, library preparation efficiency and size-selection distribution (based on Fig. 2C) of the three DNA purification methods were compared. (C) Size, quantity and quality of SMRTbell libraries before and after size-selection were assessed using the Agilent DNA 12000 kit on the Agilent 2100 Bioanalyzer System.
Figure Legend Snippet: Comparison of SMRTbell library preparation efficiency from P. falciparum genomic DNA purified using three different methods. (A) High molecular weight P. falciparum genomic DNA prepared from an asynchronous culture using the Genomic tip kit was purified by three different methods: (i) AMPure PB magnetic bead-based clean up (Lanes 1 2), (ii) electrophoretic DNA extraction using the Aurora System (Lanes 3 4) or (iii) phenol-chloroform extraction (Lanes 5 6), and sheared as described in Materials and Methods. Quality and size distribution of the sheared DNA (140 ng) was assessed using field-inversion gel electrophoresis (Pippin Pulse System, Sage Science). Size markers included CHEF 8-48 kb DNA Size Standard (Bio-Rad) and 2.5 kb Molecular Ruler (Bio-Rad). (B) SMRTbell libraries prepared using the indicated amount of purified genomic DNA was subjected to size selection on the BluePippin System using a 15 kb cut-off. The DNA yield and % recovery of various steps, library preparation efficiency and size-selection distribution (based on Fig. 2C) of the three DNA purification methods were compared. (C) Size, quantity and quality of SMRTbell libraries before and after size-selection were assessed using the Agilent DNA 12000 kit on the Agilent 2100 Bioanalyzer System.

Techniques Used: Purification, Molecular Weight, DNA Extraction, Nucleic Acid Electrophoresis, Selection, DNA Purification

9) Product Images from "Region-Specific Differences in Amyloid Precursor Protein Expression in the Mouse Hippocampus"

Article Title: Region-Specific Differences in Amyloid Precursor Protein Expression in the Mouse Hippocampus

Journal: Frontiers in Molecular Neuroscience

doi: 10.3389/fnmol.2016.00134

Quantitative App mRNA expression in hippocampal subregions. (A,B) LMD of hippocampal subregions, i.e., CA1 pcl and gcl of the DG. Representative section of the dorsal hippocampus (coronal plane, cresyl violet staining) before (A) and after (B) LMD is shown. Scale bar: 250 μm. (C) RNA integrity analysis of total RNA isolated from the dissected gcl (red) and from pcl (blue) demonstrating highly intact RNA (RIN-values: ~9; Agilent 2100 Bioanalyzer). (D,E) Average expression stability values ( M ) and evaluation of the optimum number of candidate reference genes for CA1 pcl and for DG gcl according to geNorm software. Pairwise variation (V) of candidate reference genes indicates that the use of the two most stable genes is sufficient to obtain an accurate normalization index for quantitative PCR (qPCR) analysis. (F) A significantly higher gene expression for App (1.5- to 1.7-fold) was detected in CA1 pcl relative to DG gcl using two different App -specific TaqMan assays ( A : Mm_01344172_m1, B : Mm_00431830_m1) after normalization to a reference gene index calculated by geNorm. (G,H) Gene expression stability values ( S ) and accumulated SD analysis using NormFinder. The minimal number of reference genes required for effective normalization is highlighted. (I) Comparable to the results obtained by geNorm algorithm, a significantly higher App expression (1.6- to 1.7-fold) was detected in CA1 pcl relative to DG gcl after normalization to the reference gene index estimated by NormFinder. Data ( N = 5–6 mice) were tested for statistical significance using one-way ANOVA followed by Bonferroni post hoc test to correct for multiple comparisons, * p ≤ 0.05.
Figure Legend Snippet: Quantitative App mRNA expression in hippocampal subregions. (A,B) LMD of hippocampal subregions, i.e., CA1 pcl and gcl of the DG. Representative section of the dorsal hippocampus (coronal plane, cresyl violet staining) before (A) and after (B) LMD is shown. Scale bar: 250 μm. (C) RNA integrity analysis of total RNA isolated from the dissected gcl (red) and from pcl (blue) demonstrating highly intact RNA (RIN-values: ~9; Agilent 2100 Bioanalyzer). (D,E) Average expression stability values ( M ) and evaluation of the optimum number of candidate reference genes for CA1 pcl and for DG gcl according to geNorm software. Pairwise variation (V) of candidate reference genes indicates that the use of the two most stable genes is sufficient to obtain an accurate normalization index for quantitative PCR (qPCR) analysis. (F) A significantly higher gene expression for App (1.5- to 1.7-fold) was detected in CA1 pcl relative to DG gcl using two different App -specific TaqMan assays ( A : Mm_01344172_m1, B : Mm_00431830_m1) after normalization to a reference gene index calculated by geNorm. (G,H) Gene expression stability values ( S ) and accumulated SD analysis using NormFinder. The minimal number of reference genes required for effective normalization is highlighted. (I) Comparable to the results obtained by geNorm algorithm, a significantly higher App expression (1.6- to 1.7-fold) was detected in CA1 pcl relative to DG gcl after normalization to the reference gene index estimated by NormFinder. Data ( N = 5–6 mice) were tested for statistical significance using one-way ANOVA followed by Bonferroni post hoc test to correct for multiple comparisons, * p ≤ 0.05.

Techniques Used: Expressing, Laser Capture Microdissection, Staining, Isolation, Software, Real-time Polymerase Chain Reaction, Mouse Assay

10) Product Images from "Region-Specific Differences in Amyloid Precursor Protein Expression in the Mouse Hippocampus"

Article Title: Region-Specific Differences in Amyloid Precursor Protein Expression in the Mouse Hippocampus

Journal: Frontiers in Molecular Neuroscience

doi: 10.3389/fnmol.2016.00134

Quantitative App mRNA expression in hippocampal subregions. (A,B) LMD of hippocampal subregions, i.e., CA1 pcl and gcl of the DG. Representative section of the dorsal hippocampus (coronal plane, cresyl violet staining) before (A) and after (B) LMD is shown. Scale bar: 250 μm. (C) RNA integrity analysis of total RNA isolated from the dissected gcl (red) and from pcl (blue) demonstrating highly intact RNA (RIN-values: ~9; Agilent 2100 Bioanalyzer). (D,E) Average expression stability values ( M ) and evaluation of the optimum number of candidate reference genes for CA1 pcl and for DG gcl according to geNorm software. Pairwise variation (V) of candidate reference genes indicates that the use of the two most stable genes is sufficient to obtain an accurate normalization index for quantitative PCR (qPCR) analysis. (F) A significantly higher gene expression for App (1.5- to 1.7-fold) was detected in CA1 pcl relative to DG gcl using two different App -specific TaqMan assays ( A : Mm_01344172_m1, B : Mm_00431830_m1) after normalization to a reference gene index calculated by geNorm. (G,H) Gene expression stability values ( S ) and accumulated SD analysis using NormFinder. The minimal number of reference genes required for effective normalization is highlighted. (I) Comparable to the results obtained by geNorm algorithm, a significantly higher App expression (1.6- to 1.7-fold) was detected in CA1 pcl relative to DG gcl after normalization to the reference gene index estimated by NormFinder. Data ( N = 5–6 mice) were tested for statistical significance using one-way ANOVA followed by Bonferroni post hoc test to correct for multiple comparisons, * p ≤ 0.05.
Figure Legend Snippet: Quantitative App mRNA expression in hippocampal subregions. (A,B) LMD of hippocampal subregions, i.e., CA1 pcl and gcl of the DG. Representative section of the dorsal hippocampus (coronal plane, cresyl violet staining) before (A) and after (B) LMD is shown. Scale bar: 250 μm. (C) RNA integrity analysis of total RNA isolated from the dissected gcl (red) and from pcl (blue) demonstrating highly intact RNA (RIN-values: ~9; Agilent 2100 Bioanalyzer). (D,E) Average expression stability values ( M ) and evaluation of the optimum number of candidate reference genes for CA1 pcl and for DG gcl according to geNorm software. Pairwise variation (V) of candidate reference genes indicates that the use of the two most stable genes is sufficient to obtain an accurate normalization index for quantitative PCR (qPCR) analysis. (F) A significantly higher gene expression for App (1.5- to 1.7-fold) was detected in CA1 pcl relative to DG gcl using two different App -specific TaqMan assays ( A : Mm_01344172_m1, B : Mm_00431830_m1) after normalization to a reference gene index calculated by geNorm. (G,H) Gene expression stability values ( S ) and accumulated SD analysis using NormFinder. The minimal number of reference genes required for effective normalization is highlighted. (I) Comparable to the results obtained by geNorm algorithm, a significantly higher App expression (1.6- to 1.7-fold) was detected in CA1 pcl relative to DG gcl after normalization to the reference gene index estimated by NormFinder. Data ( N = 5–6 mice) were tested for statistical significance using one-way ANOVA followed by Bonferroni post hoc test to correct for multiple comparisons, * p ≤ 0.05.

Techniques Used: Expressing, Laser Capture Microdissection, Staining, Isolation, Software, Real-time Polymerase Chain Reaction, Mouse Assay

Related Articles

Amplification:

Article Title: Targeted next generation sequencing for molecular diagnosis of Usher syndrome
Article Snippet: .. A PCR amplification of the captured target libraries was performed following the manufacturer’s instructions and, after its purification with the “AMPure XP beads” (BECKMAN CULTER Inc) , the validation and quantification of the enriched target DNA in each library was performed using the 2100 Bioanalyzer system with the High Sensitivity DNA Kit and the 2100 Expert Software (Agilent Technologies Inc, CA, USA ). .. The last step of the protocol was to pool samples for multiplexed sequencing in the Illumina sequencing platform MiSeq System (Illumina,Inc ).

Article Title: Analysis of the complementarity determining regions β-chain genomic rearrangement using high-throughput sequencing in periphery cytotoxic T lymphocytes of patients with chronic hepatitis B
Article Snippet: Quality of PCR products The quality of the amplified PCR products was tested by running on an agarose gel, which demonstrated clear bands with 400–500 bp size ( ). .. The data was further analyzed using a mimic diagram to demonstrate the sizes of the bands with using the Agilent 2100 Bioanalyzer system ( ).

Article Title: Frequencies of genetic polymorphisms related to triptans metabolism in chronic migraine
Article Snippet: .. Identification of the amplified fragments size was performed by microchannel electrophoresis on chip, using the Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA, USA). .. All the single nucleotide polymorphisms were genotyped by pyrosequencing technology (Pyrosequencer PyroMark ID system–Biotage AB and Biosystems, Uppsala, Sweden).

Article Title: Region-Specific Differences in Amyloid Precursor Protein Expression in the Mouse Hippocampus
Article Snippet: .. PCR products were checked on Agilent DNA 1000 Chips (Agilent Technologies) with the Agilent 2100 Bioanalyzer system to verify product specificity and amplicon size. .. Quantification of the gene expression of candidate reference genes was carried out using SYBR® GreenERTM qPCR Supermix Universal (Invitrogen, Waltham, MA, USA) following the manufacturer’s recommendations.

Construct:

Article Title: Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read ( > 11 kb), single molecule, real-time sequencing
Article Snippet: Each library was constructed using ∼4 μg of purified DNA and the SMRTbell Template Prep Kit 1.0, according to the protocol described in ‘Procedure & Checklist—20 kb Template Preparation Using BluePippin™ Size-Selection System’ (Pacific Biosciences). .. Library quality and quantity were assessed using the Agilent 12000 DNA Kit and 2100 Bioanalyzer System (Agilent Technologies), as well as the Qubit dsDNA Broad Range Assay kit and Qubit Fluorometer (Thermo Fisher).

Adsorption:

Article Title: Diagnostic performance of a tear protein panel in early dry eye
Article Snippet: Aspirated tears were then centrifuged at 13.200 ×g for 15 min, and the supernatant was aspirated and stored in low protein adsorption surface plastic vials at 4 °C until analysis, performed within 2 days. .. Chip-based analysis was performed with the Agilent 2100 Bioanalyzer system (Agilent, Waldbronn, Germany), using the LabChip Kit Protein 230, according to the manufacturer’s instructions, as previously described [ ].

Electrophoresis:

Article Title: Frequencies of genetic polymorphisms related to triptans metabolism in chronic migraine
Article Snippet: .. Identification of the amplified fragments size was performed by microchannel electrophoresis on chip, using the Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA, USA). .. All the single nucleotide polymorphisms were genotyped by pyrosequencing technology (Pyrosequencer PyroMark ID system–Biotage AB and Biosystems, Uppsala, Sweden).

Microarray:

Article Title: Hypothalamic stem cells control aging speed partly through exosomal miRNAs
Article Snippet: Exosomal miRNA analyses Exosomal miRNAs were analyzed according to the following approaches: 1) Exosomal small RNA bioanalyzer analysis: Exosomal RNAs were run on pico RNA chips or small RNA chips of Agilent 2100 Bioanalyzer system (agilent technologies), performed in the Molecular Pathology Platform, Herbert Irving Comprehensive Cancer Center, Columbia University, New York. .. 2) miRNA microarray: miRNAs microarray was performed on GeneChip® miRNA 4.0 Array (Affymetrix) in the Albert Einstein College of Medicine Genomics Core using exosomal small RNAs as input. miRNAs pathway analysis was performed using DIANA-mirPath v.3 online software.

Incubation:

Article Title: Persistence of Candida albicans in the Oral Mucosa Induces a Curbed Inflammatory Host Response That Is Independent of Immunosuppression
Article Snippet: Epithelial sheets were incubated in RNAlater (Sigma-Aldrich) for 1 min immediately after isolation and then grinded in liquid N2 . .. RNA integrity was determined using a 2100 Bioanalyzer system (Agilent Technologies) according to the manufacturer's instructions.

Expressing:

Article Title: Apparent bias toward long gene misregulation in MeCP2 syndromes disappears after controlling for baseline variations
Article Snippet: Paragraph title: Cerebellar gene expression from Mecp2 -null and WT mice ... RNA quality was assessed using the Agilent 2100 Bioanalyzer system prior to library preparation for deep sequencing or use of the total RNA for NanoString nCounter quantification.

Article Title: Region-Specific Differences in Amyloid Precursor Protein Expression in the Mouse Hippocampus
Article Snippet: PCR products were checked on Agilent DNA 1000 Chips (Agilent Technologies) with the Agilent 2100 Bioanalyzer system to verify product specificity and amplicon size. .. Quantification of the gene expression of candidate reference genes was carried out using SYBR® GreenERTM qPCR Supermix Universal (Invitrogen, Waltham, MA, USA) following the manufacturer’s recommendations.

PSQ Assay:

Article Title: Frequencies of genetic polymorphisms related to triptans metabolism in chronic migraine
Article Snippet: Identification of the amplified fragments size was performed by microchannel electrophoresis on chip, using the Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA, USA). .. Forward, reverse and sequencing primers were obtained by PSQ Assay Design software (Biotage AB and Biosystems, Uppsala, Sweden).

Ligation:

Article Title: Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read ( > 11 kb), single molecule, real-time sequencing
Article Snippet: Following ligation, extraneous DNA was digested with exonucleases and the SMRTbell library was cleaned and concentrated with AMPure PB beads. .. Library quality and quantity were assessed using the Agilent 12000 DNA Kit and 2100 Bioanalyzer System (Agilent Technologies), as well as the Qubit dsDNA Broad Range Assay kit and Qubit Fluorometer (Thermo Fisher).

Generated:

Article Title: Persistence of Candida albicans in the Oral Mucosa Induces a Curbed Inflammatory Host Response That Is Independent of Immunosuppression
Article Snippet: Three epithelial sheets were pooled for the generation of each RNA-seq replicate and two replicates were generated. .. RNA integrity was determined using a 2100 Bioanalyzer system (Agilent Technologies) according to the manufacturer's instructions.

Polymerase Chain Reaction:

Article Title: Targeted next generation sequencing for molecular diagnosis of Usher syndrome
Article Snippet: .. A PCR amplification of the captured target libraries was performed following the manufacturer’s instructions and, after its purification with the “AMPure XP beads” (BECKMAN CULTER Inc) , the validation and quantification of the enriched target DNA in each library was performed using the 2100 Bioanalyzer system with the High Sensitivity DNA Kit and the 2100 Expert Software (Agilent Technologies Inc, CA, USA ). .. The last step of the protocol was to pool samples for multiplexed sequencing in the Illumina sequencing platform MiSeq System (Illumina,Inc ).

Article Title: Analysis of the complementarity determining regions β-chain genomic rearrangement using high-throughput sequencing in periphery cytotoxic T lymphocytes of patients with chronic hepatitis B
Article Snippet: Paragraph title: Quality of PCR products ... The data was further analyzed using a mimic diagram to demonstrate the sizes of the bands with using the Agilent 2100 Bioanalyzer system ( ).

Article Title: Frequencies of genetic polymorphisms related to triptans metabolism in chronic migraine
Article Snippet: Identification of the amplified fragments size was performed by microchannel electrophoresis on chip, using the Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA, USA). .. PCR primer pairs and sequencing primer for each SNP are reported in Table .

Article Title: Analysis of the complementarity determining regions β-chain genomic rearrangement using high-throughput sequencing in periphery cytotoxic T lymphocytes of patients with chronic hepatitis B
Article Snippet: .. Agilent 2100 Bioanalyzer System (Agilent Technologies, Inc., Santa Clara, CA, USA) was used to determine the quality of the PCR products from the patient samples prior and subsequent to treatment. ..

Article Title: Region-Specific Differences in Amyloid Precursor Protein Expression in the Mouse Hippocampus
Article Snippet: .. PCR products were checked on Agilent DNA 1000 Chips (Agilent Technologies) with the Agilent 2100 Bioanalyzer system to verify product specificity and amplicon size. .. Quantification of the gene expression of candidate reference genes was carried out using SYBR® GreenERTM qPCR Supermix Universal (Invitrogen, Waltham, MA, USA) following the manufacturer’s recommendations.

Recombinant:

Article Title: A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins
Article Snippet: The Agilent 2100 Bioanalyzer system included protein assay analysis software and software for managing patient data. .. Commercially purified albumin, lysozyme, lactoferrin (all from Sigma), and recombinant lipophilin A and C (courtesy gift from Dr. Joerg Klug, Institut fuer Anatomie und Zellbiologie, JLU Giessen, Germany) and lipocalin (courtesy gift from Prof. Bernhard Redl, Division of Molecular Biology Biocenter-Innsbruck Medical University, Austria) were run as standards to observe and evaluate their separation and migration patterns.

RNA Sequencing Assay:

Article Title: TLR7‐let‐7 Signaling Contributes to Ethanol‐Induced Hepatic Inflammatory Response in Mice and in Alcoholic Hepatitis
Article Snippet: Paragraph title: RNA Sequencing of Human Liver Tissues ... Extracted tRNA was analyzed with the Agilent 2100 Bioanalyzer system (Agilent Biotechnologies, Palo Alto, CA).

Article Title: Apparent bias toward long gene misregulation in MeCP2 syndromes disappears after controlling for baseline variations
Article Snippet: RNA quality was assessed using the Agilent 2100 Bioanalyzer system prior to library preparation for deep sequencing or use of the total RNA for NanoString nCounter quantification. .. RNA sequencing was performed using Illumina HiSeq 2000.

Article Title: Persistence of Candida albicans in the Oral Mucosa Induces a Curbed Inflammatory Host Response That Is Independent of Immunosuppression
Article Snippet: Three epithelial sheets were pooled for the generation of each RNA-seq replicate and two replicates were generated. .. RNA integrity was determined using a 2100 Bioanalyzer system (Agilent Technologies) according to the manufacturer's instructions.

Fluorescence:

Article Title: A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins
Article Snippet: Separated proteins were detected with laser-induced fluorescence. .. The Agilent 2100 Bioanalyzer system included protein assay analysis software and software for managing patient data.

Magnetic Beads:

Article Title: Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read ( > 11 kb), single molecule, real-time sequencing
Article Snippet: Library quality and quantity were assessed using the Agilent 12000 DNA Kit and 2100 Bioanalyzer System (Agilent Technologies), as well as the Qubit dsDNA Broad Range Assay kit and Qubit Fluorometer (Thermo Fisher). .. Based on this analysis, polymerase-bound SMRTbell libraries were loaded at a concentration of 200 pM for libraries cleaned up using magnetic beads and electrophoretic extraction, and at 100 pM for the phenol-chloroform extracted library to achieve comparable sequencing efficiencies.

Isolation:

Article Title: Region-Specific Differences in Amyloid Precursor Protein Expression in the Mouse Hippocampus
Article Snippet: Paragraph title: RNA Isolation and Reverse Transcription ... RNA integrity was assessed using the Agilent 2100 Bioanalyzer system and Agilent RNA 6000 Pico Kit (Agilent Technologies), and then reverse transcribed using High Capacity cDNA Reverse Transcription Reagents Kit (Applied Biosystems) following the manufacturer’s recommendations.

Article Title: Persistence of Candida albicans in the Oral Mucosa Induces a Curbed Inflammatory Host Response That Is Independent of Immunosuppression
Article Snippet: Paragraph title: Preparation of Tongue Epithelial Sheets and RNA Isolation ... RNA integrity was determined using a 2100 Bioanalyzer system (Agilent Technologies) according to the manufacturer's instructions.

Article Title: Frequencies of genetic polymorphisms related to triptans metabolism in chronic migraine
Article Snippet: Genomic DNA was isolated from peripheral blood using the X-tractor Gene system (Corbett Life Science, Australia). .. Identification of the amplified fragments size was performed by microchannel electrophoresis on chip, using the Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA, USA).

Mouse Assay:

Article Title: Apparent bias toward long gene misregulation in MeCP2 syndromes disappears after controlling for baseline variations
Article Snippet: Paragraph title: Cerebellar gene expression from Mecp2 -null and WT mice ... RNA quality was assessed using the Agilent 2100 Bioanalyzer system prior to library preparation for deep sequencing or use of the total RNA for NanoString nCounter quantification.

Sequencing:

Article Title: TLR7‐let‐7 Signaling Contributes to Ethanol‐Induced Hepatic Inflammatory Response in Mice and in Alcoholic Hepatitis
Article Snippet: For sequencing, tRNA was extracted from healthy, AH, or NASH liver tissues using TRIzol (Affo et al., ). .. Extracted tRNA was analyzed with the Agilent 2100 Bioanalyzer system (Agilent Biotechnologies, Palo Alto, CA).

Article Title: Apparent bias toward long gene misregulation in MeCP2 syndromes disappears after controlling for baseline variations
Article Snippet: .. RNA quality was assessed using the Agilent 2100 Bioanalyzer system prior to library preparation for deep sequencing or use of the total RNA for NanoString nCounter quantification. .. RNA sequencing was performed using Illumina HiSeq 2000.

Article Title: Targeted next generation sequencing for molecular diagnosis of Usher syndrome
Article Snippet: Paragraph title: Sequence capture and next generation sequencing ... A PCR amplification of the captured target libraries was performed following the manufacturer’s instructions and, after its purification with the “AMPure XP beads” (BECKMAN CULTER Inc) , the validation and quantification of the enriched target DNA in each library was performed using the 2100 Bioanalyzer system with the High Sensitivity DNA Kit and the 2100 Expert Software (Agilent Technologies Inc, CA, USA ).

Article Title: Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read ( > 11 kb), single molecule, real-time sequencing
Article Snippet: Paragraph title: 2.4. SMRTbell library preparation and sequencing ... Library quality and quantity were assessed using the Agilent 12000 DNA Kit and 2100 Bioanalyzer System (Agilent Technologies), as well as the Qubit dsDNA Broad Range Assay kit and Qubit Fluorometer (Thermo Fisher).

Article Title: Frequencies of genetic polymorphisms related to triptans metabolism in chronic migraine
Article Snippet: Genetic analysis We studied the following DNA polymorphisms: an untranslated variable number of tandem repeats (uVNTR) of 30 basepairs located about 1.1 kb upstream of the ATG initiation codon of monoamine oxidase A (MAO A) gene; a A → C substitution and at position −163 and a G → A transition at position −3860 in the 5′noncoding region of the CYP1A2 gene, indicated as *1F allele (rs762551) and *1C allele (rs2069514), respectively; an A → G transition at position −392 in the promoter region of CYP3A4 gene, indicated as *1B allele (rs2740574) and a C → T transition at nucleotide 825 (rs5443) in the coding sequence of G protein β3 subunit (GNB3) gene which produces a truncated form of the protein. .. Identification of the amplified fragments size was performed by microchannel electrophoresis on chip, using the Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA, USA).

Sample Prep:

Article Title: A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins
Article Snippet: The sample preparation for the High Sensitivity Protein 250 kit differed only for treatment with solutions containing dimethyl sulfoxide and ethanolamine before boiling. .. The Agilent 2100 Bioanalyzer system included protein assay analysis software and software for managing patient data.

Purification:

Article Title: Apparent bias toward long gene misregulation in MeCP2 syndromes disappears after controlling for baseline variations
Article Snippet: We performed RNA extraction and purification from the cerebellum of male mice 8–9 weeks of age (three biological replicates from WT mice and three biological replicates from Mecp2 -null mice) using the Aurum™ Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad 7326830) per the manufacturer’s instructions. .. RNA quality was assessed using the Agilent 2100 Bioanalyzer system prior to library preparation for deep sequencing or use of the total RNA for NanoString nCounter quantification.

Article Title: Targeted next generation sequencing for molecular diagnosis of Usher syndrome
Article Snippet: .. A PCR amplification of the captured target libraries was performed following the manufacturer’s instructions and, after its purification with the “AMPure XP beads” (BECKMAN CULTER Inc) , the validation and quantification of the enriched target DNA in each library was performed using the 2100 Bioanalyzer system with the High Sensitivity DNA Kit and the 2100 Expert Software (Agilent Technologies Inc, CA, USA ). .. The last step of the protocol was to pool samples for multiplexed sequencing in the Illumina sequencing platform MiSeq System (Illumina,Inc ).

Article Title: Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read ( > 11 kb), single molecule, real-time sequencing
Article Snippet: Each library was constructed using ∼4 μg of purified DNA and the SMRTbell Template Prep Kit 1.0, according to the protocol described in ‘Procedure & Checklist—20 kb Template Preparation Using BluePippin™ Size-Selection System’ (Pacific Biosciences). .. Library quality and quantity were assessed using the Agilent 12000 DNA Kit and 2100 Bioanalyzer System (Agilent Technologies), as well as the Qubit dsDNA Broad Range Assay kit and Qubit Fluorometer (Thermo Fisher).

Article Title: A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins
Article Snippet: The Agilent 2100 Bioanalyzer system included protein assay analysis software and software for managing patient data. .. Commercially purified albumin, lysozyme, lactoferrin (all from Sigma), and recombinant lipophilin A and C (courtesy gift from Dr. Joerg Klug, Institut fuer Anatomie und Zellbiologie, JLU Giessen, Germany) and lipocalin (courtesy gift from Prof. Bernhard Redl, Division of Molecular Biology Biocenter-Innsbruck Medical University, Austria) were run as standards to observe and evaluate their separation and migration patterns.

Chromatin Immunoprecipitation:

Article Title: Diagnostic performance of a tear protein panel in early dry eye
Article Snippet: .. Chip-based analysis was performed with the Agilent 2100 Bioanalyzer system (Agilent, Waldbronn, Germany), using the LabChip Kit Protein 230, according to the manufacturer’s instructions, as previously described [ ]. .. All reagents were provided with the kit, including the standard protein ladder containing different proteins with known concentration and molecular weights that can be used for semiquantitative analysis.

Article Title: A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins
Article Snippet: .. Agilent 2100 Bioanalyzer system Chip-based analysis was performed with the Agilent 2100 Bioanalyzer system (Agilent, Waldbronn, Germany). ..

Article Title: Frequencies of genetic polymorphisms related to triptans metabolism in chronic migraine
Article Snippet: .. Identification of the amplified fragments size was performed by microchannel electrophoresis on chip, using the Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA, USA). .. All the single nucleotide polymorphisms were genotyped by pyrosequencing technology (Pyrosequencer PyroMark ID system–Biotage AB and Biosystems, Uppsala, Sweden).

Software:

Article Title: Targeted next generation sequencing for molecular diagnosis of Usher syndrome
Article Snippet: .. A PCR amplification of the captured target libraries was performed following the manufacturer’s instructions and, after its purification with the “AMPure XP beads” (BECKMAN CULTER Inc) , the validation and quantification of the enriched target DNA in each library was performed using the 2100 Bioanalyzer system with the High Sensitivity DNA Kit and the 2100 Expert Software (Agilent Technologies Inc, CA, USA ). .. The last step of the protocol was to pool samples for multiplexed sequencing in the Illumina sequencing platform MiSeq System (Illumina,Inc ).

Article Title: A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins
Article Snippet: .. The Agilent 2100 Bioanalyzer system included protein assay analysis software and software for managing patient data. .. Commercially purified albumin, lysozyme, lactoferrin (all from Sigma), and recombinant lipophilin A and C (courtesy gift from Dr. Joerg Klug, Institut fuer Anatomie und Zellbiologie, JLU Giessen, Germany) and lipocalin (courtesy gift from Prof. Bernhard Redl, Division of Molecular Biology Biocenter-Innsbruck Medical University, Austria) were run as standards to observe and evaluate their separation and migration patterns.

Article Title: Hypothalamic stem cells control aging speed partly through exosomal miRNAs
Article Snippet: Exosomal miRNA analyses Exosomal miRNAs were analyzed according to the following approaches: 1) Exosomal small RNA bioanalyzer analysis: Exosomal RNAs were run on pico RNA chips or small RNA chips of Agilent 2100 Bioanalyzer system (agilent technologies), performed in the Molecular Pathology Platform, Herbert Irving Comprehensive Cancer Center, Columbia University, New York. .. 2) miRNA microarray: miRNAs microarray was performed on GeneChip® miRNA 4.0 Array (Affymetrix) in the Albert Einstein College of Medicine Genomics Core using exosomal small RNAs as input. miRNAs pathway analysis was performed using DIANA-mirPath v.3 online software.

Article Title: Frequencies of genetic polymorphisms related to triptans metabolism in chronic migraine
Article Snippet: Identification of the amplified fragments size was performed by microchannel electrophoresis on chip, using the Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA, USA). .. Forward, reverse and sequencing primers were obtained by PSQ Assay Design software (Biotage AB and Biosystems, Uppsala, Sweden).

Article Title: Region-Specific Differences in Amyloid Precursor Protein Expression in the Mouse Hippocampus
Article Snippet: PCR products were checked on Agilent DNA 1000 Chips (Agilent Technologies) with the Agilent 2100 Bioanalyzer system to verify product specificity and amplicon size. .. Primer efficiencies and quantification cycle (Cq) values were calculated using LinRegPCR Software (Tuomi et al., ).

Real-time Polymerase Chain Reaction:

Article Title: Region-Specific Differences in Amyloid Precursor Protein Expression in the Mouse Hippocampus
Article Snippet: Paragraph title: Quantitative Polymerase Chain Reaction (qPCR) ... PCR products were checked on Agilent DNA 1000 Chips (Agilent Technologies) with the Agilent 2100 Bioanalyzer system to verify product specificity and amplicon size.

RNA Extraction:

Article Title: Apparent bias toward long gene misregulation in MeCP2 syndromes disappears after controlling for baseline variations
Article Snippet: We performed RNA extraction and purification from the cerebellum of male mice 8–9 weeks of age (three biological replicates from WT mice and three biological replicates from Mecp2 -null mice) using the Aurum™ Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad 7326830) per the manufacturer’s instructions. .. RNA quality was assessed using the Agilent 2100 Bioanalyzer system prior to library preparation for deep sequencing or use of the total RNA for NanoString nCounter quantification.

Agarose Gel Electrophoresis:

Article Title: Analysis of the complementarity determining regions β-chain genomic rearrangement using high-throughput sequencing in periphery cytotoxic T lymphocytes of patients with chronic hepatitis B
Article Snippet: Quality of PCR products The quality of the amplified PCR products was tested by running on an agarose gel, which demonstrated clear bands with 400–500 bp size ( ). .. The data was further analyzed using a mimic diagram to demonstrate the sizes of the bands with using the Agilent 2100 Bioanalyzer system ( ).

Next-Generation Sequencing:

Article Title: Targeted next generation sequencing for molecular diagnosis of Usher syndrome
Article Snippet: Paragraph title: Sequence capture and next generation sequencing ... A PCR amplification of the captured target libraries was performed following the manufacturer’s instructions and, after its purification with the “AMPure XP beads” (BECKMAN CULTER Inc) , the validation and quantification of the enriched target DNA in each library was performed using the 2100 Bioanalyzer system with the High Sensitivity DNA Kit and the 2100 Expert Software (Agilent Technologies Inc, CA, USA ).

Irradiation:

Article Title: Effects of Lyse-It on endonuclease fragmentation, function and activity
Article Snippet: [ , ] Therefore, we investigated the effects of increasing the oxygen content within the nuclease suspensions prior to microwave irradiation with Lyse-It. .. To be able to see the fragments, the Agilent 2100 Bioanalyzer system with the Protein 80 kit was used.

Concentration Assay:

Article Title: Diagnostic performance of a tear protein panel in early dry eye
Article Snippet: Chip-based analysis was performed with the Agilent 2100 Bioanalyzer system (Agilent, Waldbronn, Germany), using the LabChip Kit Protein 230, according to the manufacturer’s instructions, as previously described [ ]. .. All reagents were provided with the kit, including the standard protein ladder containing different proteins with known concentration and molecular weights that can be used for semiquantitative analysis.

Article Title: Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read ( > 11 kb), single molecule, real-time sequencing
Article Snippet: Library quality and quantity were assessed using the Agilent 12000 DNA Kit and 2100 Bioanalyzer System (Agilent Technologies), as well as the Qubit dsDNA Broad Range Assay kit and Qubit Fluorometer (Thermo Fisher). .. To identify the library concentration that would achieve optimal Poisson loading on the SMRT Cell (i.e. ∼40% of zero mode waveguides loaded with a single DNA molecule), loading titrations were performed for each library.

Article Title: A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins
Article Snippet: Agilent 2100 Bioanalyzer system Chip-based analysis was performed with the Agilent 2100 Bioanalyzer system (Agilent, Waldbronn, Germany). .. All reagents were provided with each LabChip kit, including the standard protein ladder containing different proteins with known concentration and molecular weights that can be used for semiquantitative analysis.

Article Title: Effects of Lyse-It on endonuclease fragmentation, function and activity
Article Snippet: Paragraph title: As oxygen concentration increased within the nuclease samples, more fragments were seen ... To be able to see the fragments, the Agilent 2100 Bioanalyzer system with the Protein 80 kit was used.

Migration:

Article Title: A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins
Article Snippet: The Agilent 2100 Bioanalyzer system included protein assay analysis software and software for managing patient data. .. Commercially purified albumin, lysozyme, lactoferrin (all from Sigma), and recombinant lipophilin A and C (courtesy gift from Dr. Joerg Klug, Institut fuer Anatomie und Zellbiologie, JLU Giessen, Germany) and lipocalin (courtesy gift from Prof. Bernhard Redl, Division of Molecular Biology Biocenter-Innsbruck Medical University, Austria) were run as standards to observe and evaluate their separation and migration patterns.

Staining:

Article Title: Diagnostic performance of a tear protein panel in early dry eye
Article Snippet: Conjunctival staining was recorded for three areas of temporal and nasal conjunctiva of each eye and graded 0–3 as above for each zone with a maximum score of 18. .. Chip-based analysis was performed with the Agilent 2100 Bioanalyzer system (Agilent, Waldbronn, Germany), using the LabChip Kit Protein 230, according to the manufacturer’s instructions, as previously described [ ].

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    Agilent technologies 2100 bioanalyzer
    Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent <t>2100</t> <t>Bioanalyzer)</t> of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated
    2100 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2100 bioanalyzer/product/Agilent technologies
    Average 99 stars, based on 148 article reviews
    Price from $9.99 to $1999.99
    2100 bioanalyzer - by Bioz Stars, 2020-01
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    Agilent technologies dna high sensitivity kit
    Analysis of plasma <t>cfDNA</t> obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent <t>DNA</t> high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.
    Dna High Sensitivity Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna high sensitivity kit/product/Agilent technologies
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    Price from $9.99 to $1999.99
    dna high sensitivity kit - by Bioz Stars, 2020-01
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    99
    Agilent technologies bioanalyzer 2100 instrument
    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent <t>Bioanalyzer</t> 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.
    Bioanalyzer 2100 Instrument, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer 2100 instrument/product/Agilent technologies
    Average 99 stars, based on 90 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer 2100 instrument - by Bioz Stars, 2020-01
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    Image Search Results


    Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Effects of fixation and postfixation processing on total cellular RNA: gel-simulated image (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Lane L: RNA ladder. Lane 1: Native non–formaldehyde-treated

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Effects of fixation and postfixation processing on total cellular RNA: gel-simulated image (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Lane L: RNA ladder. Lane 1: Native non–formaldehyde-treated

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Effects of buffer composition on postfixation processing of an RNA 50mer: gel-simulated image (Agilent 2100 Bioanalyzer). Lane L: RNA ladder. Lane 1: Native RNA 50mer. Lane 2: RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Excess formaldehyde

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Effects of buffer composition on postfixation processing of an RNA 50mer: gel-simulated image (Agilent 2100 Bioanalyzer). Lane L: RNA ladder. Lane 1: Native RNA 50mer. Lane 2: RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Excess formaldehyde

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Postformaldehyde fixation processing of an RNA 50mer. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours and then heated at 70°C for 30 minutes, where indicated, in the following

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Postformaldehyde fixation processing of an RNA 50mer. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours and then heated at 70°C for 30 minutes, where indicated, in the following

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Effects of fixation and postfixation processing on total HeLa RNA. Electropherograms (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. FU denotes fluorescence units. A: Non–formaldehyde-treated

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Effects of fixation and postfixation processing on total HeLa RNA. Electropherograms (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. FU denotes fluorescence units. A: Non–formaldehyde-treated

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques: Fluorescence

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques: