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Agilent technologies 2100 bioanalyzer instrument
Depletion of rRNA from RNA samples isolated from dual species P. aeruginosa / S. aureus cultures. A total of 2 μg of DNAse-treated RNA, isolated form a dual species P. aeruginosa PAO1 and S. aureus ATCC6538 culture, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria) or Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using the <t>Bioanalyzer</t> RNA 6000 Pico kit. Representative electropherograms of ( A ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( B ) MICROBExpress or ( C ) Ribo-Zero kits are shown. ( D ) Percent of rRNA contamination as reported by the Agilent Bioanalyzer <t>2100</t> Expert Software in RNA samples from single and dual species cultures of P. aeruginosa and/or S. aureus treated with the Ribo-Zero or MICROBExpress kits. Untreated input RNA (Total) or RNA processed via Ribo-Zero or MICROBExpress treatment was subjected to qPCR analysis of P. aeruginosa ( E , G ) or S. aureus ( F , H ) rRNA transcript abundance. ( E , F ) qPCR threshold cycle (Cq) for the indicated rRNA transcripts. ( G , H ) Copy numbers of P. aeruginosa or S. aureus 16S, 23 s, and 5S rRNA transcripts were calculated using respective qPCR standard curves. cDNA synthesis for the qPCR reactions was performed using 10 ng of indicated RNA samples as input. Experiments were repeated using two biological replicates. Error bars indicate standard deviation.
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1) Product Images from "Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes"

Article Title: Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes

Journal: Scientific Reports

doi: 10.1038/srep41114

Depletion of rRNA from RNA samples isolated from dual species P. aeruginosa / S. aureus cultures. A total of 2 μg of DNAse-treated RNA, isolated form a dual species P. aeruginosa PAO1 and S. aureus ATCC6538 culture, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria) or Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( A ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( B ) MICROBExpress or ( C ) Ribo-Zero kits are shown. ( D ) Percent of rRNA contamination as reported by the Agilent Bioanalyzer 2100 Expert Software in RNA samples from single and dual species cultures of P. aeruginosa and/or S. aureus treated with the Ribo-Zero or MICROBExpress kits. Untreated input RNA (Total) or RNA processed via Ribo-Zero or MICROBExpress treatment was subjected to qPCR analysis of P. aeruginosa ( E , G ) or S. aureus ( F , H ) rRNA transcript abundance. ( E , F ) qPCR threshold cycle (Cq) for the indicated rRNA transcripts. ( G , H ) Copy numbers of P. aeruginosa or S. aureus 16S, 23 s, and 5S rRNA transcripts were calculated using respective qPCR standard curves. cDNA synthesis for the qPCR reactions was performed using 10 ng of indicated RNA samples as input. Experiments were repeated using two biological replicates. Error bars indicate standard deviation.
Figure Legend Snippet: Depletion of rRNA from RNA samples isolated from dual species P. aeruginosa / S. aureus cultures. A total of 2 μg of DNAse-treated RNA, isolated form a dual species P. aeruginosa PAO1 and S. aureus ATCC6538 culture, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria) or Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( A ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( B ) MICROBExpress or ( C ) Ribo-Zero kits are shown. ( D ) Percent of rRNA contamination as reported by the Agilent Bioanalyzer 2100 Expert Software in RNA samples from single and dual species cultures of P. aeruginosa and/or S. aureus treated with the Ribo-Zero or MICROBExpress kits. Untreated input RNA (Total) or RNA processed via Ribo-Zero or MICROBExpress treatment was subjected to qPCR analysis of P. aeruginosa ( E , G ) or S. aureus ( F , H ) rRNA transcript abundance. ( E , F ) qPCR threshold cycle (Cq) for the indicated rRNA transcripts. ( G , H ) Copy numbers of P. aeruginosa or S. aureus 16S, 23 s, and 5S rRNA transcripts were calculated using respective qPCR standard curves. cDNA synthesis for the qPCR reactions was performed using 10 ng of indicated RNA samples as input. Experiments were repeated using two biological replicates. Error bars indicate standard deviation.

Techniques Used: Isolation, Software, Real-time Polymerase Chain Reaction, Standard Deviation

Quantitative and qualitative comparison of RNA recovered following rRNA depletion. A total of 4 μg of DNAse-treated RNA, isolated form 3-day-old P. aeruginosa PAO1 biofilms, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria), Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit and the Life Technologies RiboMinus Transcriptome Isolation Kit, Bacteria. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using Qubit fluorimetric quantitation with the Qubit RNA HS Assay Kit. Yields are reported as total RNA recovered ( A ) and as percentage of the input RNA ( B ). The RNA samples were also assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( C ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( D ) MICROBExpress, ( E ) RiboMinus, or ( F ) Ribo-Zero kits are shown. Dashed lines indicate peaks corresponding to 16S and 23S RNA traces, which were detected in total RNA, MICROBExpress, and RiboMins samples. ( G ) The area of 16S and 23S rRNA peaks as percent of the total detected RNA was estimated, as determined using the 2100 Expert Software. RFU, relative fluorescence units. Experiments were repeated using three biological replicates.
Figure Legend Snippet: Quantitative and qualitative comparison of RNA recovered following rRNA depletion. A total of 4 μg of DNAse-treated RNA, isolated form 3-day-old P. aeruginosa PAO1 biofilms, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria), Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit and the Life Technologies RiboMinus Transcriptome Isolation Kit, Bacteria. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using Qubit fluorimetric quantitation with the Qubit RNA HS Assay Kit. Yields are reported as total RNA recovered ( A ) and as percentage of the input RNA ( B ). The RNA samples were also assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( C ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( D ) MICROBExpress, ( E ) RiboMinus, or ( F ) Ribo-Zero kits are shown. Dashed lines indicate peaks corresponding to 16S and 23S RNA traces, which were detected in total RNA, MICROBExpress, and RiboMins samples. ( G ) The area of 16S and 23S rRNA peaks as percent of the total detected RNA was estimated, as determined using the 2100 Expert Software. RFU, relative fluorescence units. Experiments were repeated using three biological replicates.

Techniques Used: Isolation, Quantitation Assay, RNA HS Assay, Software, Fluorescence

2) Product Images from "Extraction of microRNAs from biological matrices with titanium dioxide nanofibers"

Article Title: Extraction of microRNAs from biological matrices with titanium dioxide nanofibers

Journal: Analytical and bioanalytical chemistry

doi: 10.1007/s00216-017-0649-3

Comparison of small RNA extraction from MDA-MB-231 cells with fibers and columns analyzed by an Agilent 2100 Bioanalyzer. Small RNA recovery was as high as 985 pg/μL with the fibers and 10.2 pg/μL with the columns from as little as 134,000
Figure Legend Snippet: Comparison of small RNA extraction from MDA-MB-231 cells with fibers and columns analyzed by an Agilent 2100 Bioanalyzer. Small RNA recovery was as high as 985 pg/μL with the fibers and 10.2 pg/μL with the columns from as little as 134,000

Techniques Used: RNA Extraction, Multiple Displacement Amplification

3) Product Images from "The Dormancy Regulator DosR Controls Ribosome Stability in Hypoxic Mycobacteria *"

Article Title: The Dormancy Regulator DosR Controls Ribosome Stability in Hypoxic Mycobacteria *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M112.364851

Loss of DosR has a profound effect on ribosome and ribosomal RNA stability. Cultures of M. smegmatis mc 2 155 wild-type and the Δ dosR mutant were grown at 37 °C to mid-exponential phase ( A ), normoxic stationary phase ( B ), or hypoxic stationary phase ( C ). Cells were harvested, lysed, and ribosomes were isolated by ultracentrifugation and fractionated through a linear sucrose gradient (15–40%). Then the A 254 and refraction were determined per fraction and plotted. D and E , for ribosomal RNA stability experiments, cultures were grown to hypoxic stationary phase at 37 °C in LB. RNA was purified using the TRIzol reagent and analyzed with RNA Nano 6000 chips on an Agilent 2100 bioanalyzer. Traces shown are the average of three independent experiments and were normalized to account for chip variability.
Figure Legend Snippet: Loss of DosR has a profound effect on ribosome and ribosomal RNA stability. Cultures of M. smegmatis mc 2 155 wild-type and the Δ dosR mutant were grown at 37 °C to mid-exponential phase ( A ), normoxic stationary phase ( B ), or hypoxic stationary phase ( C ). Cells were harvested, lysed, and ribosomes were isolated by ultracentrifugation and fractionated through a linear sucrose gradient (15–40%). Then the A 254 and refraction were determined per fraction and plotted. D and E , for ribosomal RNA stability experiments, cultures were grown to hypoxic stationary phase at 37 °C in LB. RNA was purified using the TRIzol reagent and analyzed with RNA Nano 6000 chips on an Agilent 2100 bioanalyzer. Traces shown are the average of three independent experiments and were normalized to account for chip variability.

Techniques Used: Mutagenesis, Isolation, Purification, Chromatin Immunoprecipitation

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Centrifugation:

Article Title: The Dormancy Regulator DosR Controls Ribosome Stability in Hypoxic Mycobacteria *
Article Snippet: RNA Isolation, Biosynthesis, and rRNA Profiling Cells from 50 ml of bacterial culture were harvested by centrifugation (6000 × g , 10 min at 4 °C), and RNA was isolated using TRIzol as described previously ( ). .. RNA integrity was assayed using the Agilent RNA 6000 Nano Chip kit (Agilent) and analyzed by a 2100 Bioanalyzer instrument (Agilent).

Article Title: Increased synapse elimination by microglia in schizophrenia patient-derived models of synaptic pruning
Article Snippet: Cells were collected using Accutase and pelleted by centrifugation at 300 g . .. RNA quality was further assessed on an 2100 Bioanalyzer Instrument (Agilent Technologies) and samples with RNA integrity numbers > 6 were used for analysis.

Amplification:

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Article Snippet: Several rounds of PCR amplification with PCR Primer Cocktail and PCR Master Mix were performed to enrich the cDNA fragments. .. The average molecule length was measured using the Agilent 2100 bioanalyzer instrument (Agilent DNA 1000 Reagents), and by real-time quantitative PCR (TaqMan Probe).

Article Title: Combinatorial codon scrambling enables scalable gene synthesis and amplification of repetitive proteins
Article Snippet: 1 μL of the full-length PCA product was then amplified by PCR using terminal universal primers ( ). .. The product was diluted 1:50 and then visualized with microfluidic electrophoresis using the Agilent high sensitivity DNA kit and the 2100 Bioanalyzer instrument (Agilent Technologies).

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Article Snippet: The Ovation RNA-Seq System V2 and Ovation Ultralow Library System V2 (NuGEN Technologies, Inc., San Carlos, CA, USA) were used for amplification of 100 ng of total RNA and library prep. .. For quality control, the fragmentation product and libraries were validated using a 2100 Bioanalyzer Instrument (Agilent Technologies, Santa Clara, CA, USA).

Construct:

Article Title: The draft genome sequence of the ferret (Mustela putorius furo) facilitates study of human respiratory disease
Article Snippet: The presence of 18S and 28S rRNA peaks was checked using the Agilent 2100 Bioanalyzer instrument (Agilent). .. Strand-specific mRNA libraries were constructed using the Illumina TruSeq RNA Preparation Kit (Illumina) according to the manufacturer’s guide.

Article Title: De novo transcriptome sequencing and assembly from apomictic and sexual Eragrostis curvula genotypes
Article Snippet: RNA quality was checked with a RNA 6000 Pico LabChip using the Agilent 2100 Bioanalyzer instrument (Agilent, USA). mRNA was purified using Dynabeads oligo (dT) magnetic beads (Invitrogen), following the manufacturer protocol, and quantified using an Agilent 2100 Bioanalyzer instrument (Agilent, USA). .. The cDNA library was constructed using the GS FLX Titanium Rapid Library Preparation Kit (Roche).

Electrophoresis:

Article Title: Combinatorial codon scrambling enables scalable gene synthesis and amplification of repetitive proteins
Article Snippet: .. The product was diluted 1:50 and then visualized with microfluidic electrophoresis using the Agilent high sensitivity DNA kit and the 2100 Bioanalyzer instrument (Agilent Technologies). .. BsaI recognition sites, 4 bp overhangs (CGGT/GGCT), and the His6 -tag coding region were added to a modified pET-24a(+) vector with primers, pET24a FWD/REV 1, using PCR ( ).

Microarray:

Article Title: Increased synapse elimination by microglia in schizophrenia patient-derived models of synaptic pruning
Article Snippet: Paragraph title: Global gene expression microarray hybridization and data analyses. ... RNA quality was further assessed on an 2100 Bioanalyzer Instrument (Agilent Technologies) and samples with RNA integrity numbers > 6 were used for analysis.

Incubation:

Article Title: Loss-of-function variants in ADCY3 increase risk of obesity and type 2 diabetes
Article Snippet: For the adapter ligation, Adenylate 3'Ends DNA, RNA Index Adapter and Ligation Mix, were mixed and incubated (30°C for 10 min). .. The average molecule length was measured using the Agilent 2100 bioanalyzer instrument (Agilent DNA 1000 Reagents), and by real-time quantitative PCR (TaqMan Probe).

Expressing:

Article Title: Increased synapse elimination by microglia in schizophrenia patient-derived models of synaptic pruning
Article Snippet: Paragraph title: Global gene expression microarray hybridization and data analyses. ... RNA quality was further assessed on an 2100 Bioanalyzer Instrument (Agilent Technologies) and samples with RNA integrity numbers > 6 were used for analysis.

Article Title: Microenvironmental control of breast cancer subtype elicited by paracrine platelet derived growth factor-CC signaling
Article Snippet: Samples prepared in quintuplicate biological repeats were checked for quality using a 2100 Bioanalyzer instrument (Agilent) (RIN range 9.6-10), used as templates to prepare libraries (TruSeq, Illumina), and subjected to RNA sequencing using a NextSeq instrument (Illumina). .. Kallisto (v0.43.0) was used for abundance estimation and differential expression analysis was performed with Sleuth (v0.29.0) .

Modification:

Article Title: Combinatorial codon scrambling enables scalable gene synthesis and amplification of repetitive proteins
Article Snippet: The product was diluted 1:50 and then visualized with microfluidic electrophoresis using the Agilent high sensitivity DNA kit and the 2100 Bioanalyzer instrument (Agilent Technologies). .. BsaI recognition sites, 4 bp overhangs (CGGT/GGCT), and the His6 -tag coding region were added to a modified pET-24a(+) vector with primers, pET24a FWD/REV 1, using PCR ( ).

Derivative Assay:

Article Title: De novo transcriptome sequencing and assembly from apomictic and sexual Eragrostis curvula genotypes
Article Snippet: Four RNA samples were prepared: O2P1 and O2P2 were biological replicates derived from spikelets of sexual genotype OTA-S whereas T3P1 and T3P2 were biological replicates obtained from spikelets of the full apomictic genotype Tanganyika. .. RNA quality was checked with a RNA 6000 Pico LabChip using the Agilent 2100 Bioanalyzer instrument (Agilent, USA). mRNA was purified using Dynabeads oligo (dT) magnetic beads (Invitrogen), following the manufacturer protocol, and quantified using an Agilent 2100 Bioanalyzer instrument (Agilent, USA).

Hybridization:

Article Title: Increased synapse elimination by microglia in schizophrenia patient-derived models of synaptic pruning
Article Snippet: Paragraph title: Global gene expression microarray hybridization and data analyses. ... RNA quality was further assessed on an 2100 Bioanalyzer Instrument (Agilent Technologies) and samples with RNA integrity numbers > 6 were used for analysis.

Flow Cytometry:

Article Title: Loss-of-function variants in ADCY3 increase risk of obesity and type 2 diabetes
Article Snippet: The average molecule length was measured using the Agilent 2100 bioanalyzer instrument (Agilent DNA 1000 Reagents), and by real-time quantitative PCR (TaqMan Probe). .. The amplified flow cell was sequenced paired-end on the HiSeq 4000 System (TruSeq SBS KIT-HS V3, Illumina).

Article Title: CBP/β-Catenin/FOXM1 Is a Novel Therapeutic Target in Triple Negative Breast Cancer
Article Snippet: For quality control, the fragmentation product and libraries were validated using a 2100 Bioanalyzer Instrument (Agilent Technologies, Santa Clara, CA, USA). .. FastQ files were analyzed using Partek Flow software (Partek Inc., Chesterfield, MO, USA).

Ligation:

Article Title: Loss-of-function variants in ADCY3 increase risk of obesity and type 2 diabetes
Article Snippet: For the adapter ligation, Adenylate 3'Ends DNA, RNA Index Adapter and Ligation Mix, were mixed and incubated (30°C for 10 min). .. The average molecule length was measured using the Agilent 2100 bioanalyzer instrument (Agilent DNA 1000 Reagents), and by real-time quantitative PCR (TaqMan Probe).

Article Title: Root microbiota drive direct integration of phosphate stress and immunity
Article Snippet: The DNA fragments were then adenylated using Klenow exo-polymerase to allow the ligation of Illumina Truseq HT adapters (D501–D508 and D701–D712). .. Following library preparation, quality control and quantification were performed using a 2100 Bioanalyzer instrument (Agilent) and the Quant-iT PicoGreen dsDNA Reagent (Invitrogen), respectively.

Polymerase Chain Reaction:

Article Title: Loss-of-function variants in ADCY3 increase risk of obesity and type 2 diabetes
Article Snippet: The PCR products were purified with Ampure XP Beads (AGENCOURT). .. The average molecule length was measured using the Agilent 2100 bioanalyzer instrument (Agilent DNA 1000 Reagents), and by real-time quantitative PCR (TaqMan Probe).

Article Title: Combinatorial codon scrambling enables scalable gene synthesis and amplification of repetitive proteins
Article Snippet: The 50 μL PCR reaction contained 250 μM of each dNTP, 1X Herculase II PCR buffer, 0.25 μM each of universal forward and reverse primers and 0.5 μL Herculase II Fusion polymerase. .. The product was diluted 1:50 and then visualized with microfluidic electrophoresis using the Agilent high sensitivity DNA kit and the 2100 Bioanalyzer instrument (Agilent Technologies).

Radioactivity:

Article Title: The Dormancy Regulator DosR Controls Ribosome Stability in Hypoxic Mycobacteria *
Article Snippet: Radioactivity was measured using a Wallac scintillation counter (PerkinElmer Life Sciences). .. RNA integrity was assayed using the Agilent RNA 6000 Nano Chip kit (Agilent) and analyzed by a 2100 Bioanalyzer instrument (Agilent).

RNA Sequencing Assay:

Article Title: Loss-of-function variants in ADCY3 increase risk of obesity and type 2 diabetes
Article Snippet: Paragraph title: RNA sequencing analysis ... The average molecule length was measured using the Agilent 2100 bioanalyzer instrument (Agilent DNA 1000 Reagents), and by real-time quantitative PCR (TaqMan Probe).

Article Title: The draft genome sequence of the ferret (Mustela putorius furo) facilitates study of human respiratory disease
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Article Title: Root microbiota drive direct integration of phosphate stress and immunity
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Article Title: CBP/β-Catenin/FOXM1 Is a Novel Therapeutic Target in Triple Negative Breast Cancer
Article Snippet: Paragraph title: 4.6. RNA Seq Library Preparation and Data Analysis ... For quality control, the fragmentation product and libraries were validated using a 2100 Bioanalyzer Instrument (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Microenvironmental control of breast cancer subtype elicited by paracrine platelet derived growth factor-CC signaling
Article Snippet: .. Samples prepared in quintuplicate biological repeats were checked for quality using a 2100 Bioanalyzer instrument (Agilent) (RIN range 9.6-10), used as templates to prepare libraries (TruSeq, Illumina), and subjected to RNA sequencing using a NextSeq instrument (Illumina). .. Raw sequencing data was de-multiplexed and merged into two fastq files (read 1 and read 2) for each sample using Picard tools ExtractIlluminaBarcodes and lluminaBasecallsToFastq ( https://broadinstitute.github.io/picard/ ).

Magnetic Beads:

Article Title: Root microbiota drive direct integration of phosphate stress and immunity
Article Snippet: Briefly, mRNA was purified from total RNA using Sera-mag oligo(dT) magnetic beads (GE Healthcare Life Sciences) and then fragmented in the presence of divalent cations (Mg2+ ) at 94 °C for 6 min. .. Following library preparation, quality control and quantification were performed using a 2100 Bioanalyzer instrument (Agilent) and the Quant-iT PicoGreen dsDNA Reagent (Invitrogen), respectively.

Article Title: De novo transcriptome sequencing and assembly from apomictic and sexual Eragrostis curvula genotypes
Article Snippet: .. RNA quality was checked with a RNA 6000 Pico LabChip using the Agilent 2100 Bioanalyzer instrument (Agilent, USA). mRNA was purified using Dynabeads oligo (dT) magnetic beads (Invitrogen), following the manufacturer protocol, and quantified using an Agilent 2100 Bioanalyzer instrument (Agilent, USA). .. A total of 200 ng of each RNA sample meeting the quality criterion of integrity was used for chemical fragmentation of mRNA, as recommended by the cDNA Rapid Library Preparation Method Manual (Roche).

Multiple Displacement Amplification:

Article Title: CBP/β-Catenin/FOXM1 Is a Novel Therapeutic Target in Triple Negative Breast Cancer
Article Snippet: MDA-MB-231 samples were prepared in biological duplicates after treatment for 48 h with 10 μM ICG-001 or DMSO vehicle control. .. For quality control, the fragmentation product and libraries were validated using a 2100 Bioanalyzer Instrument (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Extraction of microRNAs from biological matrices with titanium dioxide nanofibers
Article Snippet: MDA-MB-231 cells were used to test small RNA recovery efficiency of both fibers and commercial columns. .. The chip was run on an Agilent 2100 Bioanalyzer Instrument using the Small RNA Analysis Kit with 1 μL of extraction sample following the protocol provided by Agilent.

Isolation:

Article Title: The Dormancy Regulator DosR Controls Ribosome Stability in Hypoxic Mycobacteria *
Article Snippet: Paragraph title: RNA Isolation, Biosynthesis, and rRNA Profiling ... RNA integrity was assayed using the Agilent RNA 6000 Nano Chip kit (Agilent) and analyzed by a 2100 Bioanalyzer instrument (Agilent).

Article Title: The draft genome sequence of the ferret (Mustela putorius furo) facilitates study of human respiratory disease
Article Snippet: RNA extraction and library preparation Tissues used for RNA sequencing were excised and immediately immersed in RNA Later (Ambion) for 24 h at 4°C, subsequently frozen at −80°C, and later thawed and homogenized in TRIzol (Life Technologies); RNA was isolated using QIAGEN miRN easy protocols. .. The presence of 18S and 28S rRNA peaks was checked using the Agilent 2100 Bioanalyzer instrument (Agilent).

Article Title: Root microbiota drive direct integration of phosphate stress and immunity
Article Snippet: Paragraph title: RNA isolation and RNA-seq library construction ... Following library preparation, quality control and quantification were performed using a 2100 Bioanalyzer instrument (Agilent) and the Quant-iT PicoGreen dsDNA Reagent (Invitrogen), respectively.

Article Title: Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes
Article Snippet: Ribosomal RNA (rRNA) depletion A total of 4 ug RNA was subjected to rRNA depletion using the Illumina Ribo-Zero rRNA Removal Kit (Bacteria), Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit and the Life Technologies RiboMinus Transcriptome Isolation Kit, Bacteria, according to the manufacturers’ protocols. .. RNA quantity and quality post-depletion was assessed using the Agilent 2100 Bioanalyzer instrument as described above.

Article Title: Microenvironmental control of breast cancer subtype elicited by paracrine platelet derived growth factor-CC signaling
Article Snippet: Cells were treated in starvation media using 5 µM estradiol (E2758, Sigma-Aldrich) and/or 5 µM 4-OH-tamoxifen (Sigma-Aldrich) for 48 hours before RNA isolation (RNeasy® Mini Kit, #74106, Qiagen). .. Samples prepared in quintuplicate biological repeats were checked for quality using a 2100 Bioanalyzer instrument (Agilent) (RIN range 9.6-10), used as templates to prepare libraries (TruSeq, Illumina), and subjected to RNA sequencing using a NextSeq instrument (Illumina).

Article Title: De novo transcriptome sequencing and assembly from apomictic and sexual Eragrostis curvula genotypes
Article Snippet: Paragraph title: RNA isolation and preparation ... RNA quality was checked with a RNA 6000 Pico LabChip using the Agilent 2100 Bioanalyzer instrument (Agilent, USA). mRNA was purified using Dynabeads oligo (dT) magnetic beads (Invitrogen), following the manufacturer protocol, and quantified using an Agilent 2100 Bioanalyzer instrument (Agilent, USA).

Labeling:

Article Title: Increased synapse elimination by microglia in schizophrenia patient-derived models of synaptic pruning
Article Snippet: RNA quality was further assessed on an 2100 Bioanalyzer Instrument (Agilent Technologies) and samples with RNA integrity numbers > 6 were used for analysis. .. Quality control was performed using the manufacturer’s software Transcriptome Analysis Console version 4.0 (Thermo Fisher Scientific), according to the manufacturer’s instructions (principal component analysis, labeling controls, hybridization controls, positive versus negative area under the curve, and signal box plot) with no indication of outliers.

Purification:

Article Title: Loss-of-function variants in ADCY3 increase risk of obesity and type 2 diabetes
Article Snippet: The PCR products were purified with Ampure XP Beads (AGENCOURT). .. The average molecule length was measured using the Agilent 2100 bioanalyzer instrument (Agilent DNA 1000 Reagents), and by real-time quantitative PCR (TaqMan Probe).

Article Title: Root microbiota drive direct integration of phosphate stress and immunity
Article Snippet: Briefly, mRNA was purified from total RNA using Sera-mag oligo(dT) magnetic beads (GE Healthcare Life Sciences) and then fragmented in the presence of divalent cations (Mg2+ ) at 94 °C for 6 min. .. Following library preparation, quality control and quantification were performed using a 2100 Bioanalyzer instrument (Agilent) and the Quant-iT PicoGreen dsDNA Reagent (Invitrogen), respectively.

Article Title: De novo transcriptome sequencing and assembly from apomictic and sexual Eragrostis curvula genotypes
Article Snippet: .. RNA quality was checked with a RNA 6000 Pico LabChip using the Agilent 2100 Bioanalyzer instrument (Agilent, USA). mRNA was purified using Dynabeads oligo (dT) magnetic beads (Invitrogen), following the manufacturer protocol, and quantified using an Agilent 2100 Bioanalyzer instrument (Agilent, USA). .. A total of 200 ng of each RNA sample meeting the quality criterion of integrity was used for chemical fragmentation of mRNA, as recommended by the cDNA Rapid Library Preparation Method Manual (Roche).

Sequencing:

Article Title: Combinatorial codon scrambling enables scalable gene synthesis and amplification of repetitive proteins
Article Snippet: This entire sequence was additionally flanked by forward and reverse universal primer recognition sequences for the purpose of downstream PCR amplification. .. The product was diluted 1:50 and then visualized with microfluidic electrophoresis using the Agilent high sensitivity DNA kit and the 2100 Bioanalyzer instrument (Agilent Technologies).

Article Title: CBP/β-Catenin/FOXM1 Is a Novel Therapeutic Target in Triple Negative Breast Cancer
Article Snippet: For quality control, the fragmentation product and libraries were validated using a 2100 Bioanalyzer Instrument (Agilent Technologies, Santa Clara, CA, USA). .. Whole transcriptome RNA Seq was performed on an Illumina HiSeq 2000 (Illumina, San Diego, CA, USA) using paired-end sequencing yielding 40–60 × coverage per sample.

Article Title: Microenvironmental control of breast cancer subtype elicited by paracrine platelet derived growth factor-CC signaling
Article Snippet: Samples prepared in quintuplicate biological repeats were checked for quality using a 2100 Bioanalyzer instrument (Agilent) (RIN range 9.6-10), used as templates to prepare libraries (TruSeq, Illumina), and subjected to RNA sequencing using a NextSeq instrument (Illumina). .. Raw sequencing data was de-multiplexed and merged into two fastq files (read 1 and read 2) for each sample using Picard tools ExtractIlluminaBarcodes and lluminaBasecallsToFastq ( https://broadinstitute.github.io/picard/ ).

Article Title: High-quality genome sequences of uncultured microbes by assembly of read clouds
Article Snippet: Libraries were prepared for sequencing with the 10x Genomics Chromium (10x Genomics, Pleasanton, CA), the Illumina Truseq SLR kit, or Illumina Truseq Nano kit according to the respective manufacturer’s standard protocol. .. Library fragment size was quantified with the Agilent 2100 Bioanalyzer instrument (Agilent Technologies, Santa Clara, CA) using the High Sensitivity DNA kit.

cDNA Library Assay:

Article Title: De novo transcriptome sequencing and assembly from apomictic and sexual Eragrostis curvula genotypes
Article Snippet: RNA quality was checked with a RNA 6000 Pico LabChip using the Agilent 2100 Bioanalyzer instrument (Agilent, USA). mRNA was purified using Dynabeads oligo (dT) magnetic beads (Invitrogen), following the manufacturer protocol, and quantified using an Agilent 2100 Bioanalyzer instrument (Agilent, USA). .. The cDNA library was constructed using the GS FLX Titanium Rapid Library Preparation Kit (Roche).

Chromatin Immunoprecipitation:

Article Title: The Dormancy Regulator DosR Controls Ribosome Stability in Hypoxic Mycobacteria *
Article Snippet: .. RNA integrity was assayed using the Agilent RNA 6000 Nano Chip kit (Agilent) and analyzed by a 2100 Bioanalyzer instrument (Agilent). ..

Article Title: De novo transcriptome sequencing and assembly from apomictic and sexual Eragrostis curvula genotypes
Article Snippet: RNA quality was checked with a RNA 6000 Pico LabChip using the Agilent 2100 Bioanalyzer instrument (Agilent, USA). mRNA was purified using Dynabeads oligo (dT) magnetic beads (Invitrogen), following the manufacturer protocol, and quantified using an Agilent 2100 Bioanalyzer instrument (Agilent, USA). .. Fragmentation of the samples was checked by comparison with non-fragmented samples after running on the RNA 6000 Pico Chip on the Agilent 2100 Bioanalyzer.

Article Title: Extraction of microRNAs from biological matrices with titanium dioxide nanofibers
Article Snippet: .. The chip was run on an Agilent 2100 Bioanalyzer Instrument using the Small RNA Analysis Kit with 1 μL of extraction sample following the protocol provided by Agilent. .. The commercial column extraction protocol that was followed was the optimized protocol provided by Life Technologies for cell lysate with the provided buffers.

Plasmid Preparation:

Article Title: Combinatorial codon scrambling enables scalable gene synthesis and amplification of repetitive proteins
Article Snippet: The product was diluted 1:50 and then visualized with microfluidic electrophoresis using the Agilent high sensitivity DNA kit and the 2100 Bioanalyzer instrument (Agilent Technologies). .. BsaI recognition sites, 4 bp overhangs (CGGT/GGCT), and the His6 -tag coding region were added to a modified pET-24a(+) vector with primers, pET24a FWD/REV 1, using PCR ( ).

Software:

Article Title: CBP/β-Catenin/FOXM1 Is a Novel Therapeutic Target in Triple Negative Breast Cancer
Article Snippet: For quality control, the fragmentation product and libraries were validated using a 2100 Bioanalyzer Instrument (Agilent Technologies, Santa Clara, CA, USA). .. FastQ files were analyzed using Partek Flow software (Partek Inc., Chesterfield, MO, USA).

Article Title: Increased synapse elimination by microglia in schizophrenia patient-derived models of synaptic pruning
Article Snippet: RNA quality was further assessed on an 2100 Bioanalyzer Instrument (Agilent Technologies) and samples with RNA integrity numbers > 6 were used for analysis. .. Quality control was performed using the manufacturer’s software Transcriptome Analysis Console version 4.0 (Thermo Fisher Scientific), according to the manufacturer’s instructions (principal component analysis, labeling controls, hybridization controls, positive versus negative area under the curve, and signal box plot) with no indication of outliers.

Real-time Polymerase Chain Reaction:

Article Title: Loss-of-function variants in ADCY3 increase risk of obesity and type 2 diabetes
Article Snippet: .. The average molecule length was measured using the Agilent 2100 bioanalyzer instrument (Agilent DNA 1000 Reagents), and by real-time quantitative PCR (TaqMan Probe). .. The qualified libraries were amplified on cBot to generate the cluster on the flowcell (TruSeq PE Cluster Kit V3–cBot–HS, Illumina).

Article Title: The draft genome sequence of the ferret (Mustela putorius furo) facilitates study of human respiratory disease
Article Snippet: The presence of 18S and 28S rRNA peaks was checked using the Agilent 2100 Bioanalyzer instrument (Agilent). .. Both libraries were quality controlled and quantitated using the Agilent 2100 Bioanalyzer instrument and qPCR (Kapa Biosystems).

RNA Extraction:

Article Title: The draft genome sequence of the ferret (Mustela putorius furo) facilitates study of human respiratory disease
Article Snippet: Paragraph title: RNA extraction and library preparation ... The presence of 18S and 28S rRNA peaks was checked using the Agilent 2100 Bioanalyzer instrument (Agilent).

Positron Emission Tomography:

Article Title: Combinatorial codon scrambling enables scalable gene synthesis and amplification of repetitive proteins
Article Snippet: The product was diluted 1:50 and then visualized with microfluidic electrophoresis using the Agilent high sensitivity DNA kit and the 2100 Bioanalyzer instrument (Agilent Technologies). .. BsaI recognition sites, 4 bp overhangs (CGGT/GGCT), and the His6 -tag coding region were added to a modified pET-24a(+) vector with primers, pET24a FWD/REV 1, using PCR ( ).

Quantitation Assay:

Article Title: High-quality genome sequences of uncultured microbes by assembly of read clouds
Article Snippet: DNA concentration was measured using Qubit fluorometric quantitation (see for measured concentrations). .. Library fragment size was quantified with the Agilent 2100 Bioanalyzer instrument (Agilent Technologies, Santa Clara, CA) using the High Sensitivity DNA kit.

Article Title: Extraction of microRNAs from biological matrices with titanium dioxide nanofibers
Article Snippet: Paragraph title: MiRNA extraction and quantitation ... The chip was run on an Agilent 2100 Bioanalyzer Instrument using the Small RNA Analysis Kit with 1 μL of extraction sample following the protocol provided by Agilent.

Spectrophotometry:

Article Title: Increased synapse elimination by microglia in schizophrenia patient-derived models of synaptic pruning
Article Snippet: Total RNA was extracted using the RNeasy Mini kit (QIAGEN) according to the manufacturer’s instructions and concentrations; 260/280 nm readings were determined using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific). .. RNA quality was further assessed on an 2100 Bioanalyzer Instrument (Agilent Technologies) and samples with RNA integrity numbers > 6 were used for analysis.

Concentration Assay:

Article Title: The Dormancy Regulator DosR Controls Ribosome Stability in Hypoxic Mycobacteria *
Article Snippet: RNA synthesis was assessed by adding L -[5,6-3 H]uracil (PerkinElmer Life Sciences) to a final concentration of 0.5 μCi/ml to cultures. .. RNA integrity was assayed using the Agilent RNA 6000 Nano Chip kit (Agilent) and analyzed by a 2100 Bioanalyzer instrument (Agilent).

Article Title: High-quality genome sequences of uncultured microbes by assembly of read clouds
Article Snippet: DNA concentration was measured using Qubit fluorometric quantitation (see for measured concentrations). .. Library fragment size was quantified with the Agilent 2100 Bioanalyzer instrument (Agilent Technologies, Santa Clara, CA) using the High Sensitivity DNA kit.

Lysis:

Article Title: Extraction of microRNAs from biological matrices with titanium dioxide nanofibers
Article Snippet: The cells were lysed with RIPA lysis buffer (Santa Cruz Biotechnology) prior to proceeding with each extraction protocol, and 50 μL cell lysate, containing about 105 cells, was used per extraction. .. The chip was run on an Agilent 2100 Bioanalyzer Instrument using the Small RNA Analysis Kit with 1 μL of extraction sample following the protocol provided by Agilent.

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    Agilent technologies dna high sensitivity kit
    Analysis of plasma <t>cfDNA</t> obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent <t>DNA</t> high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.
    Dna High Sensitivity Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna high sensitivity kit/product/Agilent technologies
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    dna high sensitivity kit - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies bioanalyzer 2100 instrument
    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent <t>Bioanalyzer</t> 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.
    Bioanalyzer 2100 Instrument, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer 2100 instrument/product/Agilent technologies
    Average 99 stars, based on 90 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer 2100 instrument - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

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    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques: