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Agilent technologies 2100 bioanalyser
2100 Bioanalyser, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2100 bioanalyser/product/Agilent technologies
Average 99 stars, based on 59 article reviews
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2100 bioanalyser - by Bioz Stars, 2020-01
99/100 stars

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Centrifugation:

Article Title: The Impact of 18 Ancestral and Horizontally-Acquired Regulatory Proteins upon the Transcriptome and sRNA Landscape of Salmonella enterica serovar Typhimurium
Article Snippet: The RNA pellet was rinsed with 70% ethanol followed by centrifugation at 13,000 rpm for 10 minutes at room temperature. .. RNA quality was assessed using a 2100 Bioanalyser (Agilent).

Amplification:

Article Title: Next generation sequencing in a large cohort of patients presenting with neuromuscular disease before or at birth
Article Snippet: .. The amplified library was again purified with AMPure beads and analysed on a 2100 Bioanalyser (Agilent Technologies Genomics). .. Libraries were diluted to 18-26pM and attached to Ion Sphere™ Particles using an Ion Proton™ Template 200 v3 kit and sequenced on a P1 sequencing chip on an Ion Proton sequencer™ (Ion Sequencing 200 kit v3) in pools of two.

Article Title: Tumour cell-derived Wnt7a recruits and activates fibroblasts to promote tumour aggressiveness
Article Snippet: RNA was analysed using the RNA 6000 Nano kit on a 2100 BioAnalyser (Agilent), and only samples with an RNA integrity number > 9 were used for further experiments. .. For each tumour cell and fibroblast population, 100 ng of RNA from three independent biological replicates was amplified using the TotalPrep 96-RNA amplification kit from Ambion (Applied Biosystems). cRNA (1.6 μg) was hybridized to the MouseWG-6 v2.0 Expression BeadChip (Illumina).

Article Title: Myoglobinopathy is an adult-onset autosomal dominant myopathy with characteristic sarcoplasmic inclusions
Article Snippet: .. The final amplified library was purified again using AMPure beads and analyzed on a 2100 Bioanalyser (Agilent Technologies). ..

Article Title: Immunopathology of childhood celiac disease—Key role of intestinal epithelial cells
Article Snippet: Four hundred ng of total RNA with OD260/OD280 ratios ≥1.8, was converted to biotinylated double stranded cRNA by in vitro transcription and amplification according to the protocol of the Illumina Totalprep RNA Amplification Kit (Ambion, Austin, TX). .. Agarose gel analysis of sample integrity in a 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA) showed cRNAs suitable for hybridization with normal distribution of fragments between 200 and 6,000 base pairs in length.

Cytometry:

Article Title: Tumour cell-derived Wnt7a recruits and activates fibroblasts to promote tumour aggressiveness
Article Snippet: For isolation of tumour cells and fibroblasts, primary tumours in Ub-GFP Balb/c mice were removed at an average diameter of 10 mm, homogenized using a McIlwain Tissue Chopper (Campden Instruments) and digested in L-15 medium containing 3 mg ml−1 collagenase type I at 37 °C for 1 h, followed by digestion with 0.025 mg ml−1 DNase at 37 °C for 5 min. After erythrocyte lysis using Red Blood Cell Lysing Buffer (Sigma), single cells were resuspended at 1–2 × 107 cells per ml in PBS and stained with CD45-PE-Cy5 and 4,6-diamidino-2-phenylindole for FACSorting using a FACSAria flow cytometer (BD Biosciences). .. RNA was analysed using the RNA 6000 Nano kit on a 2100 BioAnalyser (Agilent), and only samples with an RNA integrity number > 9 were used for further experiments.

Quantitative RT-PCR:

Article Title: Evidence for ACTN3 as a genetic modifier of Duchenne muscular dystrophy
Article Snippet: Paragraph title: Quantitative real-time PCR (RT-qPCR) ... RNA quality and quantity was assessed using a 2100 Bioanalyser (Agilent Technologies) 6,000 RNA kit (Agilent Technologies) according to the manufacturer's instructions.

Article Title: Recruitment of dorsal midbrain catecholaminergic pathways in the recovery from nerve injury evoked disabilities
Article Snippet: Paragraph title: Real-time RT-PCR ... RNA concentrations were calculated using spectrophotometry (ND-1000 spectrophotometer, NanodropTH, Wilmington, DE, USA) and RNA integrity was verified using the 2100 Bioanalyser (Agilent Technologies™, Santa Clara, CA, USA).

Real-time Polymerase Chain Reaction:

Article Title: Evidence for ACTN3 as a genetic modifier of Duchenne muscular dystrophy
Article Snippet: Paragraph title: Quantitative real-time PCR (RT-qPCR) ... RNA quality and quantity was assessed using a 2100 Bioanalyser (Agilent Technologies) 6,000 RNA kit (Agilent Technologies) according to the manufacturer's instructions.

Microarray:

Article Title: SOX2 recruits KLF4 to regulate nasopharyngeal carcinoma proliferation via PI3K/AKT signaling
Article Snippet: Paragraph title: Microarray assay ... Then, RNA integrity was evaluated under the 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA, USA).

Article Title: XBP1 signalling is essential for alleviating mutant protein aggregation in ER-stress related skeletal disease
Article Snippet: Paragraph title: Microarray analysis of rib chondrocytes ... RNA integrity was analysed on the 2100 Bioanalyser (Agilent Technologies).

Incubation:

Article Title: Immunopathology of childhood celiac disease—Key role of intestinal epithelial cells
Article Snippet: Agarose gel analysis of sample integrity in a 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA) showed cRNAs suitable for hybridization with normal distribution of fragments between 200 and 6,000 base pairs in length. .. The labeled cRNA samples were then hybridized individually to Sentrix HumanRef-8_v3 expression BeadChip (18,401 genes; IEC and CD3+ IEL samples) or HumanHT-12 v4 BeadChip (34,602 genes; T84 tight monolayer samples) incubated with streptavidin-Cy3 and scanned on the Illumina BeadStation GX (both types of BeadChip and the BeadStation were from Illumina, San Diego, CA).

Expressing:

Article Title: RFX transcription factors are essential for hearing in mice
Article Snippet: RNA integrity was evaluated with a RNA Pico Chip on a 2100 Bioanalyser (Agilent). .. All samples used for gene expression analysis had a RNA integrity score > 8.5.

Article Title: Tumour cell-derived Wnt7a recruits and activates fibroblasts to promote tumour aggressiveness
Article Snippet: Paragraph title: Gene expression profiling of cell populations from tumours ... RNA was analysed using the RNA 6000 Nano kit on a 2100 BioAnalyser (Agilent), and only samples with an RNA integrity number > 9 were used for further experiments.

Article Title: Mammary stem cells have myoepithelial cell properties
Article Snippet: Gene expression profiles were determined for each group using a MouseWG-6 v2.0 Expression BeadChip (Illumina). .. Quality control: Total RNA was quality checked using a 2100 Bioanalyser (Agilent).

Article Title: Recruitment of dorsal midbrain catecholaminergic pathways in the recovery from nerve injury evoked disabilities
Article Snippet: RNA concentrations were calculated using spectrophotometry (ND-1000 spectrophotometer, NanodropTH, Wilmington, DE, USA) and RNA integrity was verified using the 2100 Bioanalyser (Agilent Technologies™, Santa Clara, CA, USA). .. Relative expression levels of TH mRNA were determined using TaqMan® FAM probe with TAMRA as the quencher dye (Rn00561369_m1).

Article Title: Immunopathology of childhood celiac disease—Key role of intestinal epithelial cells
Article Snippet: Paragraph title: Gene expression analysis using genome-wide hybridization bead array screening of cRNA libraries ... Agarose gel analysis of sample integrity in a 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA) showed cRNAs suitable for hybridization with normal distribution of fragments between 200 and 6,000 base pairs in length.

Article Title: XBP1 signalling is essential for alleviating mutant protein aggregation in ER-stress related skeletal disease
Article Snippet: RNA integrity was analysed on the 2100 Bioanalyser (Agilent Technologies). .. A GeneChip 3’ expression assay (Mouse430_2 Affymetrix) was used to analyse gene expression.

Genome Wide:

Article Title: Immunopathology of childhood celiac disease—Key role of intestinal epithelial cells
Article Snippet: Paragraph title: Gene expression analysis using genome-wide hybridization bead array screening of cRNA libraries ... Agarose gel analysis of sample integrity in a 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA) showed cRNAs suitable for hybridization with normal distribution of fragments between 200 and 6,000 base pairs in length.

Modification:

Article Title: LTR retroelements are intrinsic components of transcriptional networks in frogs
Article Snippet: Q32866) and a 2100 BioAnalyser (Agilent Technologies, Cat.No. .. RS-122-2001) with a modification of the protocol allowing to preserve directional information about the transcripts [ ].

Transformation Assay:

Article Title: Tumour cell-derived Wnt7a recruits and activates fibroblasts to promote tumour aggressiveness
Article Snippet: RNA was analysed using the RNA 6000 Nano kit on a 2100 BioAnalyser (Agilent), and only samples with an RNA integrity number > 9 were used for further experiments. .. Data were transformed using variance stabilization and normalized using quantile normalization.

Blocking Assay:

Article Title: Recruitment of dorsal midbrain catecholaminergic pathways in the recovery from nerve injury evoked disabilities
Article Snippet: The rostral boundary of this block was at the approximate coronal level of −5.3 mm caudal to bregma and the caudal boundary at approximately −8.8 mm caudal to bregma [ ]. .. RNA concentrations were calculated using spectrophotometry (ND-1000 spectrophotometer, NanodropTH, Wilmington, DE, USA) and RNA integrity was verified using the 2100 Bioanalyser (Agilent Technologies™, Santa Clara, CA, USA).

Hybridization:

Article Title: SOX2 recruits KLF4 to regulate nasopharyngeal carcinoma proliferation via PI3K/AKT signaling
Article Snippet: Then, RNA integrity was evaluated under the 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA, USA). .. Subsequently, hybridization was performed overnight on a 22K human genome array chip, V1.0 (CapitalBio Corp., Beijing, China), based on the manufacturer’s instructions.

Article Title: Immunopathology of childhood celiac disease—Key role of intestinal epithelial cells
Article Snippet: .. Agarose gel analysis of sample integrity in a 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA) showed cRNAs suitable for hybridization with normal distribution of fragments between 200 and 6,000 base pairs in length. .. The labeled cRNA samples were then hybridized individually to Sentrix HumanRef-8_v3 expression BeadChip (18,401 genes; IEC and CD3+ IEL samples) or HumanHT-12 v4 BeadChip (34,602 genes; T84 tight monolayer samples) incubated with streptavidin-Cy3 and scanned on the Illumina BeadStation GX (both types of BeadChip and the BeadStation were from Illumina, San Diego, CA).

Flow Cytometry:

Article Title: Tumour cell-derived Wnt7a recruits and activates fibroblasts to promote tumour aggressiveness
Article Snippet: For isolation of tumour cells and fibroblasts, primary tumours in Ub-GFP Balb/c mice were removed at an average diameter of 10 mm, homogenized using a McIlwain Tissue Chopper (Campden Instruments) and digested in L-15 medium containing 3 mg ml−1 collagenase type I at 37 °C for 1 h, followed by digestion with 0.025 mg ml−1 DNase at 37 °C for 5 min. After erythrocyte lysis using Red Blood Cell Lysing Buffer (Sigma), single cells were resuspended at 1–2 × 107 cells per ml in PBS and stained with CD45-PE-Cy5 and 4,6-diamidino-2-phenylindole for FACSorting using a FACSAria flow cytometer (BD Biosciences). .. RNA was analysed using the RNA 6000 Nano kit on a 2100 BioAnalyser (Agilent), and only samples with an RNA integrity number > 9 were used for further experiments.

Cell Culture:

Article Title: Mammary stem cells have myoepithelial cell properties
Article Snippet: Cultured basal cells were sorted to exclude fibroblast feeder cells. .. Quality control: Total RNA was quality checked using a 2100 Bioanalyser (Agilent).

Article Title: SOX2 recruits KLF4 to regulate nasopharyngeal carcinoma proliferation via PI3K/AKT signaling
Article Snippet: Microarray assay Total RNA was extracted from cultured cells by using the TRIzol reagent (Invitrogen, CA) and purified by using NucleoSpin® RNA clean-up Kit (Macherey-Nagel, Germany). .. Then, RNA integrity was evaluated under the 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA, USA).

Polymerase Chain Reaction:

Article Title: Next generation sequencing in a large cohort of patients presenting with neuromuscular disease before or at birth
Article Snippet: For AmpliSeq exome sequencing, 100 ng of DNA from the probands was amplified in 12 PCR pools and sequencing adaptors ligated. .. The amplified library was again purified with AMPure beads and analysed on a 2100 Bioanalyser (Agilent Technologies Genomics).

Article Title: Myoglobinopathy is an adult-onset autosomal dominant myopathy with characteristic sarcoplasmic inclusions
Article Snippet: In brief, a total of 100 ng of DNA was amplified in 12 separate PCR pools, each containing ~ 25,000 primer pairs under the following conditions: 99 °C for 2 min followed by 10 cycles of 99 °C for 15 s and 60 °C for 16 min. After amplification, the individual reactions were pooled and digested using the supplied FuPa enzyme that degrades the PCR primers. .. The final amplified library was purified again using AMPure beads and analyzed on a 2100 Bioanalyser (Agilent Technologies).

Injection:

Article Title: Tumour cell-derived Wnt7a recruits and activates fibroblasts to promote tumour aggressiveness
Article Snippet: Overall, 5 × 105 4T1, 410.4 or 4T07 tumour cells suspended in 50 μl of a 1:1 mixture of PBS and growth factor-reduced Matrigel were injected into the fourth mammary fat pad of 6–8-week-old female wild-type Balb/c or Ub-GFP Balb/c mice (Balb/c mice expressing GFP under the human ubiquitin C promoter) . .. RNA was analysed using the RNA 6000 Nano kit on a 2100 BioAnalyser (Agilent), and only samples with an RNA integrity number > 9 were used for further experiments.

RNA Sequencing Assay:

Article Title: RFX transcription factors are essential for hearing in mice
Article Snippet: RNA extraction For the samples processed by RNA-seq, TRIzol LS Reagent (Life Technologies) was added to the sorted cells (3 vol of reagent per 1 vol of sample) immediately following sorting after the completion of the post sort analysis. .. RNA integrity was evaluated with a RNA Pico Chip on a 2100 Bioanalyser (Agilent).

Article Title: Amino acid sequence conservation of the algesic MBP fragment is required for its interaction with CDK5 and function in pain
Article Snippet: Paragraph title: RNA-seq and system biology analysis ... RNA integrity was determined using 2100 Bioanalyser (Agilent Technologies).

Mutagenesis:

Article Title: XBP1 signalling is essential for alleviating mutant protein aggregation in ER-stress related skeletal disease
Article Snippet: Wild type and mutant RNA was pooled from 3 separate extractions, and the submitted to the Genomic Technologies Core Facility, University of Manchester for analysis. .. RNA integrity was analysed on the 2100 Bioanalyser (Agilent Technologies).

Isolation:

Article Title: Evidence for ACTN3 as a genetic modifier of Duchenne muscular dystrophy
Article Snippet: Quantitative real-time PCR (RT-qPCR) Total RNA was isolated from ∼50 mg of mouse quadriceps tissue using TRIzol (Invitrogen) according to the manufacturer's instructions. .. RNA quality and quantity was assessed using a 2100 Bioanalyser (Agilent Technologies) 6,000 RNA kit (Agilent Technologies) according to the manufacturer's instructions.

Article Title: The Impact of 18 Ancestral and Horizontally-Acquired Regulatory Proteins upon the Transcriptome and sRNA Landscape of Salmonella enterica serovar Typhimurium
Article Snippet: Paragraph title: Isolation of total RNA from S . Typhimurium 4/74 ... RNA quality was assessed using a 2100 Bioanalyser (Agilent).

Article Title: Tumour cell-derived Wnt7a recruits and activates fibroblasts to promote tumour aggressiveness
Article Snippet: For isolation of tumour cells and fibroblasts, primary tumours in Ub-GFP Balb/c mice were removed at an average diameter of 10 mm, homogenized using a McIlwain Tissue Chopper (Campden Instruments) and digested in L-15 medium containing 3 mg ml−1 collagenase type I at 37 °C for 1 h, followed by digestion with 0.025 mg ml−1 DNase at 37 °C for 5 min. After erythrocyte lysis using Red Blood Cell Lysing Buffer (Sigma), single cells were resuspended at 1–2 × 107 cells per ml in PBS and stained with CD45-PE-Cy5 and 4,6-diamidino-2-phenylindole for FACSorting using a FACSAria flow cytometer (BD Biosciences). .. RNA was analysed using the RNA 6000 Nano kit on a 2100 BioAnalyser (Agilent), and only samples with an RNA integrity number > 9 were used for further experiments.

Article Title: LTR retroelements are intrinsic components of transcriptional networks in frogs
Article Snippet: Paragraph title: Tissue preparation, RNA isolation, and de novo sequencing ... Q32866) and a 2100 BioAnalyser (Agilent Technologies, Cat.No.

Article Title: Recruitment of dorsal midbrain catecholaminergic pathways in the recovery from nerve injury evoked disabilities
Article Snippet: The PAG region surrounding the mesencephalic aqueduct was dissected free under a binocular microscope, weighed and total RNA of the isolated tissue was extracted using TRI reagent (Sigma-Aldrich). .. RNA concentrations were calculated using spectrophotometry (ND-1000 spectrophotometer, NanodropTH, Wilmington, DE, USA) and RNA integrity was verified using the 2100 Bioanalyser (Agilent Technologies™, Santa Clara, CA, USA).

Article Title: Amino acid sequence conservation of the algesic MBP fragment is required for its interaction with CDK5 and function in pain
Article Snippet: RNA integrity was determined using 2100 Bioanalyser (Agilent Technologies). .. Poly(A) RNA was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs).

Article Title: XBP1 signalling is essential for alleviating mutant protein aggregation in ER-stress related skeletal disease
Article Snippet: Microarray analysis of rib chondrocytes RNA was extracted from isolated chondrocytes using Trizol reagent, according to the manufacturer’s protocol (Life Technologies). .. RNA integrity was analysed on the 2100 Bioanalyser (Agilent Technologies).

Microscopy:

Article Title: Recruitment of dorsal midbrain catecholaminergic pathways in the recovery from nerve injury evoked disabilities
Article Snippet: The PAG region surrounding the mesencephalic aqueduct was dissected free under a binocular microscope, weighed and total RNA of the isolated tissue was extracted using TRI reagent (Sigma-Aldrich). .. RNA concentrations were calculated using spectrophotometry (ND-1000 spectrophotometer, NanodropTH, Wilmington, DE, USA) and RNA integrity was verified using the 2100 Bioanalyser (Agilent Technologies™, Santa Clara, CA, USA).

Purification:

Article Title: Evidence for ACTN3 as a genetic modifier of Duchenne muscular dystrophy
Article Snippet: The RNA was resuspended into 50 μl DEPC treated water and purified and DNAseI treated using RNeasy Mini on-column clean-up (Qiagen). .. RNA quality and quantity was assessed using a 2100 Bioanalyser (Agilent Technologies) 6,000 RNA kit (Agilent Technologies) according to the manufacturer's instructions.

Article Title: The Impact of 18 Ancestral and Horizontally-Acquired Regulatory Proteins upon the Transcriptome and sRNA Landscape of Salmonella enterica serovar Typhimurium
Article Snippet: RNA quality was assessed using a 2100 Bioanalyser (Agilent). .. RNA to be used for cDNA library preparations was treated with 10 units of DNase I to remove any DNA present in the sample and samples were purified by phenol-chloroform extraction.

Article Title: Next generation sequencing in a large cohort of patients presenting with neuromuscular disease before or at birth
Article Snippet: .. The amplified library was again purified with AMPure beads and analysed on a 2100 Bioanalyser (Agilent Technologies Genomics). .. Libraries were diluted to 18-26pM and attached to Ion Sphere™ Particles using an Ion Proton™ Template 200 v3 kit and sequenced on a P1 sequencing chip on an Ion Proton sequencer™ (Ion Sequencing 200 kit v3) in pools of two.

Article Title: Mammary stem cells have myoepithelial cell properties
Article Snippet: Purified basal cells sorted from the same pooled sample were split into 3 groups: pre-culture for immediate RNA extraction, 1-day-culture and 7-day-culture. .. Quality control: Total RNA was quality checked using a 2100 Bioanalyser (Agilent).

Article Title: Myoglobinopathy is an adult-onset autosomal dominant myopathy with characteristic sarcoplasmic inclusions
Article Snippet: .. The final amplified library was purified again using AMPure beads and analyzed on a 2100 Bioanalyser (Agilent Technologies). ..

Article Title: SOX2 recruits KLF4 to regulate nasopharyngeal carcinoma proliferation via PI3K/AKT signaling
Article Snippet: Microarray assay Total RNA was extracted from cultured cells by using the TRIzol reagent (Invitrogen, CA) and purified by using NucleoSpin® RNA clean-up Kit (Macherey-Nagel, Germany). .. Then, RNA integrity was evaluated under the 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA, USA).

Article Title: Immunopathology of childhood celiac disease—Key role of intestinal epithelial cells
Article Snippet: Gene expression analysis using genome-wide hybridization bead array screening of cRNA libraries Four samples of purified IECs from duodenal mucosa of CD patients with active disease, 4 samples of purified IECs of clinical controls, 5 samples of purified CD3+ IELs from CD patients with active disease and 3 samples of purified CD3+ IELs of clinical controls as well as T84 polarized tight monolayers challenged with bacteria or sham-treated were individually subjected to genome-wide hybridization bead array analysis for gene expression. .. Agarose gel analysis of sample integrity in a 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA) showed cRNAs suitable for hybridization with normal distribution of fragments between 200 and 6,000 base pairs in length.

Article Title: Amino acid sequence conservation of the algesic MBP fragment is required for its interaction with CDK5 and function in pain
Article Snippet: Total RNA was extracted from Schwann cells using TRI reagent (Zymo Research) and purified using Direct-zol microRNA system (Zymo Research). .. RNA integrity was determined using 2100 Bioanalyser (Agilent Technologies).

Sequencing:

Article Title: Next generation sequencing in a large cohort of patients presenting with neuromuscular disease before or at birth
Article Snippet: Paragraph title: Exome sequencing ... The amplified library was again purified with AMPure beads and analysed on a 2100 Bioanalyser (Agilent Technologies Genomics).

Article Title: LTR retroelements are intrinsic components of transcriptional networks in frogs
Article Snippet: Paragraph title: Tissue preparation, RNA isolation, and de novo sequencing ... Q32866) and a 2100 BioAnalyser (Agilent Technologies, Cat.No.

Article Title: Myoglobinopathy is an adult-onset autosomal dominant myopathy with characteristic sarcoplasmic inclusions
Article Snippet: Next, barcoded sequencing adaptors were ligated and the library was purified using AMPure beads (Beckman Coulter) and amplified for five cycles using Platinum high-fidelity polymerase. .. The final amplified library was purified again using AMPure beads and analyzed on a 2100 Bioanalyser (Agilent Technologies).

Labeling:

Article Title: Immunopathology of childhood celiac disease—Key role of intestinal epithelial cells
Article Snippet: Agarose gel analysis of sample integrity in a 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA) showed cRNAs suitable for hybridization with normal distribution of fragments between 200 and 6,000 base pairs in length. .. The labeled cRNA samples were then hybridized individually to Sentrix HumanRef-8_v3 expression BeadChip (18,401 genes; IEC and CD3+ IEL samples) or HumanHT-12 v4 BeadChip (34,602 genes; T84 tight monolayer samples) incubated with streptavidin-Cy3 and scanned on the Illumina BeadStation GX (both types of BeadChip and the BeadStation were from Illumina, San Diego, CA).

Staining:

Article Title: Tumour cell-derived Wnt7a recruits and activates fibroblasts to promote tumour aggressiveness
Article Snippet: For isolation of tumour cells and fibroblasts, primary tumours in Ub-GFP Balb/c mice were removed at an average diameter of 10 mm, homogenized using a McIlwain Tissue Chopper (Campden Instruments) and digested in L-15 medium containing 3 mg ml−1 collagenase type I at 37 °C for 1 h, followed by digestion with 0.025 mg ml−1 DNase at 37 °C for 5 min. After erythrocyte lysis using Red Blood Cell Lysing Buffer (Sigma), single cells were resuspended at 1–2 × 107 cells per ml in PBS and stained with CD45-PE-Cy5 and 4,6-diamidino-2-phenylindole for FACSorting using a FACSAria flow cytometer (BD Biosciences). .. RNA was analysed using the RNA 6000 Nano kit on a 2100 BioAnalyser (Agilent), and only samples with an RNA integrity number > 9 were used for further experiments.

cDNA Library Assay:

Article Title: The Impact of 18 Ancestral and Horizontally-Acquired Regulatory Proteins upon the Transcriptome and sRNA Landscape of Salmonella enterica serovar Typhimurium
Article Snippet: RNA quality was assessed using a 2100 Bioanalyser (Agilent). .. RNA to be used for cDNA library preparations was treated with 10 units of DNase I to remove any DNA present in the sample and samples were purified by phenol-chloroform extraction.

Sample Prep:

Article Title: LTR retroelements are intrinsic components of transcriptional networks in frogs
Article Snippet: Q32866) and a 2100 BioAnalyser (Agilent Technologies, Cat.No. .. MRNA-seq libraries were prepared from 2000 ng of total RNA using TruSeq RNA Sample Prep Kit v2 (Illumina, Cat.No.

Chloramphenicol Acetyltransferase Assay:

Article Title: LTR retroelements are intrinsic components of transcriptional networks in frogs
Article Snippet: .. Q32866) and a 2100 BioAnalyser (Agilent Technologies, Cat.No. ..

Mouse Assay:

Article Title: Tumour cell-derived Wnt7a recruits and activates fibroblasts to promote tumour aggressiveness
Article Snippet: For isolation of tumour cells and fibroblasts, primary tumours in Ub-GFP Balb/c mice were removed at an average diameter of 10 mm, homogenized using a McIlwain Tissue Chopper (Campden Instruments) and digested in L-15 medium containing 3 mg ml−1 collagenase type I at 37 °C for 1 h, followed by digestion with 0.025 mg ml−1 DNase at 37 °C for 5 min. After erythrocyte lysis using Red Blood Cell Lysing Buffer (Sigma), single cells were resuspended at 1–2 × 107 cells per ml in PBS and stained with CD45-PE-Cy5 and 4,6-diamidino-2-phenylindole for FACSorting using a FACSAria flow cytometer (BD Biosciences). .. RNA was analysed using the RNA 6000 Nano kit on a 2100 BioAnalyser (Agilent), and only samples with an RNA integrity number > 9 were used for further experiments.

Article Title: Mammary stem cells have myoepithelial cell properties
Article Snippet: Keywords: Cell Type: mouse mammary basal cells Experimental design: Mammary glands from 6 C57BL/6J mice were pooled for each experiment. .. Quality control: Total RNA was quality checked using a 2100 Bioanalyser (Agilent).

Chromatin Immunoprecipitation:

Article Title: RFX transcription factors are essential for hearing in mice
Article Snippet: .. RNA integrity was evaluated with a RNA Pico Chip on a 2100 Bioanalyser (Agilent). .. For the samples processed by microarrays, RNA was extracted using the RNeasy Plus Micro Kit (Qiagen).

Article Title: Next generation sequencing in a large cohort of patients presenting with neuromuscular disease before or at birth
Article Snippet: The amplified library was again purified with AMPure beads and analysed on a 2100 Bioanalyser (Agilent Technologies Genomics). .. Libraries were diluted to 18-26pM and attached to Ion Sphere™ Particles using an Ion Proton™ Template 200 v3 kit and sequenced on a P1 sequencing chip on an Ion Proton sequencer™ (Ion Sequencing 200 kit v3) in pools of two.

Article Title: SOX2 recruits KLF4 to regulate nasopharyngeal carcinoma proliferation via PI3K/AKT signaling
Article Snippet: Then, RNA integrity was evaluated under the 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA, USA). .. Subsequently, hybridization was performed overnight on a 22K human genome array chip, V1.0 (CapitalBio Corp., Beijing, China), based on the manufacturer’s instructions.

Software:

Article Title: SOX2 recruits KLF4 to regulate nasopharyngeal carcinoma proliferation via PI3K/AKT signaling
Article Snippet: Then, RNA integrity was evaluated under the 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA, USA). .. After that, the hybridization images were obtained using the LuxScan 10KA dual-channel laser scanner and digitized with LuxScan 3.0 image analysis software (CapitalBio Corp.).

Article Title: Immunopathology of childhood celiac disease—Key role of intestinal epithelial cells
Article Snippet: Agarose gel analysis of sample integrity in a 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA) showed cRNAs suitable for hybridization with normal distribution of fragments between 200 and 6,000 base pairs in length. .. The raw data was analyzed using Illumina Beadstudio software (version 3.1).

RNA Extraction:

Article Title: RFX transcription factors are essential for hearing in mice
Article Snippet: Paragraph title: RNA extraction ... RNA integrity was evaluated with a RNA Pico Chip on a 2100 Bioanalyser (Agilent).

Article Title: Transcriptome profiling of caspase-2 deficient EμMyc and Th-MYCN mouse tumors identifies distinct putative roles for caspase-2 in neuronal differentiation and immune signaling
Article Snippet: Paragraph title: RNA extraction ... RNA samples were quality tested using a 2100 Bioanalyser (Agilent Technologies) to confirm RNA integrity number (RIN) > 7.

Article Title: Tumour cell-derived Wnt7a recruits and activates fibroblasts to promote tumour aggressiveness
Article Snippet: Sorted cells were pelleted and directly lysed in TRIzol (Invitrogen) for RNA extraction. .. RNA was analysed using the RNA 6000 Nano kit on a 2100 BioAnalyser (Agilent), and only samples with an RNA integrity number > 9 were used for further experiments.

Article Title: Mammary stem cells have myoepithelial cell properties
Article Snippet: Purified basal cells sorted from the same pooled sample were split into 3 groups: pre-culture for immediate RNA extraction, 1-day-culture and 7-day-culture. .. Quality control: Total RNA was quality checked using a 2100 Bioanalyser (Agilent).

Selection:

Article Title: Tumour cell-derived Wnt7a recruits and activates fibroblasts to promote tumour aggressiveness
Article Snippet: RNA was analysed using the RNA 6000 Nano kit on a 2100 BioAnalyser (Agilent), and only samples with an RNA integrity number > 9 were used for further experiments. .. Expression data were analysed in R ( www.bioconductor.org ), using the lumi package for probe selection.

Agarose Gel Electrophoresis:

Article Title: Immunopathology of childhood celiac disease—Key role of intestinal epithelial cells
Article Snippet: .. Agarose gel analysis of sample integrity in a 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA) showed cRNAs suitable for hybridization with normal distribution of fragments between 200 and 6,000 base pairs in length. .. The labeled cRNA samples were then hybridized individually to Sentrix HumanRef-8_v3 expression BeadChip (18,401 genes; IEC and CD3+ IEL samples) or HumanHT-12 v4 BeadChip (34,602 genes; T84 tight monolayer samples) incubated with streptavidin-Cy3 and scanned on the Illumina BeadStation GX (both types of BeadChip and the BeadStation were from Illumina, San Diego, CA).

In Vitro:

Article Title: Immunopathology of childhood celiac disease—Key role of intestinal epithelial cells
Article Snippet: Four hundred ng of total RNA with OD260/OD280 ratios ≥1.8, was converted to biotinylated double stranded cRNA by in vitro transcription and amplification according to the protocol of the Illumina Totalprep RNA Amplification Kit (Ambion, Austin, TX). .. Agarose gel analysis of sample integrity in a 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA) showed cRNAs suitable for hybridization with normal distribution of fragments between 200 and 6,000 base pairs in length.

Next-Generation Sequencing:

Article Title: Myoglobinopathy is an adult-onset autosomal dominant myopathy with characteristic sarcoplasmic inclusions
Article Snippet: Molecular genetics—the two Spanish families Targeted capture and next generation sequencing (ProtonTM, Life Technologies) of 336 genes including 254 neuromuscular disease genes listed in the Neuromuscular Disorders Gene Table at the end of December 2012 ( www.musclegenetable.fr ) was performed in probands from families F1 and F2. .. The final amplified library was purified again using AMPure beads and analyzed on a 2100 Bioanalyser (Agilent Technologies).

Spectrophotometry:

Article Title: Transcriptome profiling of caspase-2 deficient EμMyc and Th-MYCN mouse tumors identifies distinct putative roles for caspase-2 in neuronal differentiation and immune signaling
Article Snippet: RNA was resuspended in diethylpyrocarbonate-(DEPC) treated water and quantified using a NanoDrop1000™ spectrophotometer (Thermo Scientific). .. RNA samples were quality tested using a 2100 Bioanalyser (Agilent Technologies) to confirm RNA integrity number (RIN) > 7.

Article Title: Recruitment of dorsal midbrain catecholaminergic pathways in the recovery from nerve injury evoked disabilities
Article Snippet: .. RNA concentrations were calculated using spectrophotometry (ND-1000 spectrophotometer, NanodropTH, Wilmington, DE, USA) and RNA integrity was verified using the 2100 Bioanalyser (Agilent Technologies™, Santa Clara, CA, USA). .. 1 μg of total RNA was used for cDNA synthesis (Tetro Reverse transcriptase, Bioline, Australia).

Article Title: Immunopathology of childhood celiac disease—Key role of intestinal epithelial cells
Article Snippet: Concentrations and purity of total RNA samples were determined by measuring optical density at 260 nm (OD260) and OD280 in a NanoDrop 1000 spectrophotometer V3.0.0 (Saveen Werner, Limhamn Sweden). .. Agarose gel analysis of sample integrity in a 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA) showed cRNAs suitable for hybridization with normal distribution of fragments between 200 and 6,000 base pairs in length.

Article Title: Amino acid sequence conservation of the algesic MBP fragment is required for its interaction with CDK5 and function in pain
Article Snippet: The RNA purity was estimated by measuring the OD260/280 ratio using a ND-1000 spectrophotometer (ThermoFisher Scientific). .. RNA integrity was determined using 2100 Bioanalyser (Agilent Technologies).

Lysis:

Article Title: Tumour cell-derived Wnt7a recruits and activates fibroblasts to promote tumour aggressiveness
Article Snippet: For isolation of tumour cells and fibroblasts, primary tumours in Ub-GFP Balb/c mice were removed at an average diameter of 10 mm, homogenized using a McIlwain Tissue Chopper (Campden Instruments) and digested in L-15 medium containing 3 mg ml−1 collagenase type I at 37 °C for 1 h, followed by digestion with 0.025 mg ml−1 DNase at 37 °C for 5 min. After erythrocyte lysis using Red Blood Cell Lysing Buffer (Sigma), single cells were resuspended at 1–2 × 107 cells per ml in PBS and stained with CD45-PE-Cy5 and 4,6-diamidino-2-phenylindole for FACSorting using a FACSAria flow cytometer (BD Biosciences). .. RNA was analysed using the RNA 6000 Nano kit on a 2100 BioAnalyser (Agilent), and only samples with an RNA integrity number > 9 were used for further experiments.

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    Agilent technologies 2100 bioanalyser system
    Depicts the integrity of RNA isolated at 30 °C from different Coffea arabica tissues (mature and immature leaf, fruit, root) and from leaves belonging to 3 different coffee species, Coffea arabica, C. canephora and C. eugenoides . The isolated RNA were analysed by electrophoretic separation using 2% agarose gel ( A ) and the Agilent <t>2100</t> <t>bioanalyser</t> system with the RNA 6000 Nano ™ kits ( B ). The total RNA fraction contained ribosomal RNA, mRNA and small RNA had a RIN value ranging between 7.5 and 8.5.
    2100 Bioanalyser System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Depicts the integrity of RNA isolated at 30 °C from different Coffea arabica tissues (mature and immature leaf, fruit, root) and from leaves belonging to 3 different coffee species, Coffea arabica, C. canephora and C. eugenoides . The isolated RNA were analysed by electrophoretic separation using 2% agarose gel ( A ) and the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits ( B ). The total RNA fraction contained ribosomal RNA, mRNA and small RNA had a RIN value ranging between 7.5 and 8.5.

    Journal: Scientific Reports

    Article Title: A single-step method for RNA isolation from tropical crops in the field

    doi: 10.1038/srep38368

    Figure Lengend Snippet: Depicts the integrity of RNA isolated at 30 °C from different Coffea arabica tissues (mature and immature leaf, fruit, root) and from leaves belonging to 3 different coffee species, Coffea arabica, C. canephora and C. eugenoides . The isolated RNA were analysed by electrophoretic separation using 2% agarose gel ( A ) and the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits ( B ). The total RNA fraction contained ribosomal RNA, mRNA and small RNA had a RIN value ranging between 7.5 and 8.5.

    Article Snippet: A spectrophotometric analysis and electrophoretic separation using agarose and the Agilent 2100 bioanalyser system using more than 120 samples confirmed RNA quality with an A 260/280 ratio of 1.84 ± 0.1 and a RNA integrity number (RIN) value of 8–9.

    Techniques: Isolation, Agarose Gel Electrophoresis

    Comparison of the effectiveness of RNAzol RT (RNA extraction in field) and TRIzol Reagent or spin-columns kit (RNA extraction in laboratory with recommended cold conditions) to isolate total RNA. ( A ) Electrophoretic separation using 2% agarose gel of RNA extracted from the same four samples using the three methods. ( B ) Electropherogram and Electrophoresis of representative sample of RNA analysed with the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits. The white arrow shows genomic DNA contaminants.

    Journal: Scientific Reports

    Article Title: A single-step method for RNA isolation from tropical crops in the field

    doi: 10.1038/srep38368

    Figure Lengend Snippet: Comparison of the effectiveness of RNAzol RT (RNA extraction in field) and TRIzol Reagent or spin-columns kit (RNA extraction in laboratory with recommended cold conditions) to isolate total RNA. ( A ) Electrophoretic separation using 2% agarose gel of RNA extracted from the same four samples using the three methods. ( B ) Electropherogram and Electrophoresis of representative sample of RNA analysed with the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits. The white arrow shows genomic DNA contaminants.

    Article Snippet: A spectrophotometric analysis and electrophoretic separation using agarose and the Agilent 2100 bioanalyser system using more than 120 samples confirmed RNA quality with an A 260/280 ratio of 1.84 ± 0.1 and a RNA integrity number (RIN) value of 8–9.

    Techniques: RNA Extraction, Agarose Gel Electrophoresis, Electrophoresis

    Depicts the integrity of RNA isolated at 30 °C from leaf tissue samples from Coffea arabica, Manihot esculenta, Oryza sativa and Zea mais . ( A ) The left-hand side of the figure shows the electrophoresis analysis by agarose gel. ( B ) The right-hand side of the figure shows the Agilent 2100 bioanalyser electrophoresis for each sample. The total RNA fraction had a RIN value ranging between 8 and 9.

    Journal: Scientific Reports

    Article Title: A single-step method for RNA isolation from tropical crops in the field

    doi: 10.1038/srep38368

    Figure Lengend Snippet: Depicts the integrity of RNA isolated at 30 °C from leaf tissue samples from Coffea arabica, Manihot esculenta, Oryza sativa and Zea mais . ( A ) The left-hand side of the figure shows the electrophoresis analysis by agarose gel. ( B ) The right-hand side of the figure shows the Agilent 2100 bioanalyser electrophoresis for each sample. The total RNA fraction had a RIN value ranging between 8 and 9.

    Article Snippet: A spectrophotometric analysis and electrophoretic separation using agarose and the Agilent 2100 bioanalyser system using more than 120 samples confirmed RNA quality with an A 260/280 ratio of 1.84 ± 0.1 and a RNA integrity number (RIN) value of 8–9.

    Techniques: Isolation, Electrophoresis, Agarose Gel Electrophoresis