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Agilent technologies 2100 bioanalyser system
Depicts the integrity of RNA isolated at 30 °C from different Coffea arabica tissues (mature and immature leaf, fruit, root) and from leaves belonging to 3 different coffee species, Coffea arabica, C. canephora and C. eugenoides . The isolated RNA were analysed by electrophoretic separation using 2% agarose gel ( A ) and the Agilent <t>2100</t> <t>bioanalyser</t> system with the RNA 6000 Nano ™ kits ( B ). The total RNA fraction contained ribosomal RNA, mRNA and small RNA had a RIN value ranging between 7.5 and 8.5.
2100 Bioanalyser System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2100 bioanalyser system/product/Agilent technologies
Average 88 stars, based on 8 article reviews
Price from $9.99 to $1999.99
2100 bioanalyser system - by Bioz Stars, 2020-07
88/100 stars

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1) Product Images from "A single-step method for RNA isolation from tropical crops in the field"

Article Title: A single-step method for RNA isolation from tropical crops in the field

Journal: Scientific Reports

doi: 10.1038/srep38368

Depicts the integrity of RNA isolated at 30 °C from different Coffea arabica tissues (mature and immature leaf, fruit, root) and from leaves belonging to 3 different coffee species, Coffea arabica, C. canephora and C. eugenoides . The isolated RNA were analysed by electrophoretic separation using 2% agarose gel ( A ) and the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits ( B ). The total RNA fraction contained ribosomal RNA, mRNA and small RNA had a RIN value ranging between 7.5 and 8.5.
Figure Legend Snippet: Depicts the integrity of RNA isolated at 30 °C from different Coffea arabica tissues (mature and immature leaf, fruit, root) and from leaves belonging to 3 different coffee species, Coffea arabica, C. canephora and C. eugenoides . The isolated RNA were analysed by electrophoretic separation using 2% agarose gel ( A ) and the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits ( B ). The total RNA fraction contained ribosomal RNA, mRNA and small RNA had a RIN value ranging between 7.5 and 8.5.

Techniques Used: Isolation, Agarose Gel Electrophoresis

Comparison of the effectiveness of RNAzol RT (RNA extraction in field) and TRIzol Reagent or spin-columns kit (RNA extraction in laboratory with recommended cold conditions) to isolate total RNA. ( A ) Electrophoretic separation using 2% agarose gel of RNA extracted from the same four samples using the three methods. ( B ) Electropherogram and Electrophoresis of representative sample of RNA analysed with the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits. The white arrow shows genomic DNA contaminants.
Figure Legend Snippet: Comparison of the effectiveness of RNAzol RT (RNA extraction in field) and TRIzol Reagent or spin-columns kit (RNA extraction in laboratory with recommended cold conditions) to isolate total RNA. ( A ) Electrophoretic separation using 2% agarose gel of RNA extracted from the same four samples using the three methods. ( B ) Electropherogram and Electrophoresis of representative sample of RNA analysed with the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits. The white arrow shows genomic DNA contaminants.

Techniques Used: RNA Extraction, Agarose Gel Electrophoresis, Electrophoresis

Depicts the integrity of RNA isolated at 30 °C from leaf tissue samples from Coffea arabica, Manihot esculenta, Oryza sativa and Zea mais . ( A ) The left-hand side of the figure shows the electrophoresis analysis by agarose gel. ( B ) The right-hand side of the figure shows the Agilent 2100 bioanalyser electrophoresis for each sample. The total RNA fraction had a RIN value ranging between 8 and 9.
Figure Legend Snippet: Depicts the integrity of RNA isolated at 30 °C from leaf tissue samples from Coffea arabica, Manihot esculenta, Oryza sativa and Zea mais . ( A ) The left-hand side of the figure shows the electrophoresis analysis by agarose gel. ( B ) The right-hand side of the figure shows the Agilent 2100 bioanalyser electrophoresis for each sample. The total RNA fraction had a RIN value ranging between 8 and 9.

Techniques Used: Isolation, Electrophoresis, Agarose Gel Electrophoresis

2) Product Images from "A single-step method for RNA isolation from tropical crops in the field"

Article Title: A single-step method for RNA isolation from tropical crops in the field

Journal: Scientific Reports

doi: 10.1038/srep38368

Depicts the integrity of RNA isolated at 30 °C from different Coffea arabica tissues (mature and immature leaf, fruit, root) and from leaves belonging to 3 different coffee species, Coffea arabica, C. canephora and C. eugenoides . The isolated RNA were analysed by electrophoretic separation using 2% agarose gel ( A ) and the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits ( B ). The total RNA fraction contained ribosomal RNA, mRNA and small RNA had a RIN value ranging between 7.5 and 8.5.
Figure Legend Snippet: Depicts the integrity of RNA isolated at 30 °C from different Coffea arabica tissues (mature and immature leaf, fruit, root) and from leaves belonging to 3 different coffee species, Coffea arabica, C. canephora and C. eugenoides . The isolated RNA were analysed by electrophoretic separation using 2% agarose gel ( A ) and the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits ( B ). The total RNA fraction contained ribosomal RNA, mRNA and small RNA had a RIN value ranging between 7.5 and 8.5.

Techniques Used: Isolation, Agarose Gel Electrophoresis

Comparison of the effectiveness of RNAzol RT (RNA extraction in field) and TRIzol Reagent or spin-columns kit (RNA extraction in laboratory with recommended cold conditions) to isolate total RNA. ( A ) Electrophoretic separation using 2% agarose gel of RNA extracted from the same four samples using the three methods. ( B ) Electropherogram and Electrophoresis of representative sample of RNA analysed with the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits. The white arrow shows genomic DNA contaminants.
Figure Legend Snippet: Comparison of the effectiveness of RNAzol RT (RNA extraction in field) and TRIzol Reagent or spin-columns kit (RNA extraction in laboratory with recommended cold conditions) to isolate total RNA. ( A ) Electrophoretic separation using 2% agarose gel of RNA extracted from the same four samples using the three methods. ( B ) Electropherogram and Electrophoresis of representative sample of RNA analysed with the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits. The white arrow shows genomic DNA contaminants.

Techniques Used: RNA Extraction, Agarose Gel Electrophoresis, Electrophoresis

Depicts the integrity of RNA isolated at 30 °C from leaf tissue samples from Coffea arabica, Manihot esculenta, Oryza sativa and Zea mais . ( A ) The left-hand side of the figure shows the electrophoresis analysis by agarose gel. ( B ) The right-hand side of the figure shows the Agilent 2100 bioanalyser electrophoresis for each sample. The total RNA fraction had a RIN value ranging between 8 and 9.
Figure Legend Snippet: Depicts the integrity of RNA isolated at 30 °C from leaf tissue samples from Coffea arabica, Manihot esculenta, Oryza sativa and Zea mais . ( A ) The left-hand side of the figure shows the electrophoresis analysis by agarose gel. ( B ) The right-hand side of the figure shows the Agilent 2100 bioanalyser electrophoresis for each sample. The total RNA fraction had a RIN value ranging between 8 and 9.

Techniques Used: Isolation, Electrophoresis, Agarose Gel Electrophoresis

3) Product Images from "A single-step method for RNA isolation from tropical crops in the field"

Article Title: A single-step method for RNA isolation from tropical crops in the field

Journal: Scientific Reports

doi: 10.1038/srep38368

Depicts the integrity of RNA isolated at 30 °C from different Coffea arabica tissues (mature and immature leaf, fruit, root) and from leaves belonging to 3 different coffee species, Coffea arabica, C. canephora and C. eugenoides . The isolated RNA were analysed by electrophoretic separation using 2% agarose gel ( A ) and the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits ( B ). The total RNA fraction contained ribosomal RNA, mRNA and small RNA had a RIN value ranging between 7.5 and 8.5.
Figure Legend Snippet: Depicts the integrity of RNA isolated at 30 °C from different Coffea arabica tissues (mature and immature leaf, fruit, root) and from leaves belonging to 3 different coffee species, Coffea arabica, C. canephora and C. eugenoides . The isolated RNA were analysed by electrophoretic separation using 2% agarose gel ( A ) and the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits ( B ). The total RNA fraction contained ribosomal RNA, mRNA and small RNA had a RIN value ranging between 7.5 and 8.5.

Techniques Used: Isolation, Agarose Gel Electrophoresis

Comparison of the effectiveness of RNAzol RT (RNA extraction in field) and TRIzol Reagent or spin-columns kit (RNA extraction in laboratory with recommended cold conditions) to isolate total RNA. ( A ) Electrophoretic separation using 2% agarose gel of RNA extracted from the same four samples using the three methods. ( B ) Electropherogram and Electrophoresis of representative sample of RNA analysed with the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits. The white arrow shows genomic DNA contaminants.
Figure Legend Snippet: Comparison of the effectiveness of RNAzol RT (RNA extraction in field) and TRIzol Reagent or spin-columns kit (RNA extraction in laboratory with recommended cold conditions) to isolate total RNA. ( A ) Electrophoretic separation using 2% agarose gel of RNA extracted from the same four samples using the three methods. ( B ) Electropherogram and Electrophoresis of representative sample of RNA analysed with the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits. The white arrow shows genomic DNA contaminants.

Techniques Used: RNA Extraction, Agarose Gel Electrophoresis, Electrophoresis

Depicts the integrity of RNA isolated at 30 °C from leaf tissue samples from Coffea arabica, Manihot esculenta, Oryza sativa and Zea mais . ( A ) The left-hand side of the figure shows the electrophoresis analysis by agarose gel. ( B ) The right-hand side of the figure shows the Agilent 2100 bioanalyser electrophoresis for each sample. The total RNA fraction had a RIN value ranging between 8 and 9.
Figure Legend Snippet: Depicts the integrity of RNA isolated at 30 °C from leaf tissue samples from Coffea arabica, Manihot esculenta, Oryza sativa and Zea mais . ( A ) The left-hand side of the figure shows the electrophoresis analysis by agarose gel. ( B ) The right-hand side of the figure shows the Agilent 2100 bioanalyser electrophoresis for each sample. The total RNA fraction had a RIN value ranging between 8 and 9.

Techniques Used: Isolation, Electrophoresis, Agarose Gel Electrophoresis

4) Product Images from "A single-step method for RNA isolation from tropical crops in the field"

Article Title: A single-step method for RNA isolation from tropical crops in the field

Journal: Scientific Reports

doi: 10.1038/srep38368

Depicts the integrity of RNA isolated at 30 °C from different Coffea arabica tissues (mature and immature leaf, fruit, root) and from leaves belonging to 3 different coffee species, Coffea arabica, C. canephora and C. eugenoides . The isolated RNA were analysed by electrophoretic separation using 2% agarose gel ( A ) and the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits ( B ). The total RNA fraction contained ribosomal RNA, mRNA and small RNA had a RIN value ranging between 7.5 and 8.5.
Figure Legend Snippet: Depicts the integrity of RNA isolated at 30 °C from different Coffea arabica tissues (mature and immature leaf, fruit, root) and from leaves belonging to 3 different coffee species, Coffea arabica, C. canephora and C. eugenoides . The isolated RNA were analysed by electrophoretic separation using 2% agarose gel ( A ) and the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits ( B ). The total RNA fraction contained ribosomal RNA, mRNA and small RNA had a RIN value ranging between 7.5 and 8.5.

Techniques Used: Isolation, Agarose Gel Electrophoresis

Comparison of the effectiveness of RNAzol RT (RNA extraction in field) and TRIzol Reagent or spin-columns kit (RNA extraction in laboratory with recommended cold conditions) to isolate total RNA. ( A ) Electrophoretic separation using 2% agarose gel of RNA extracted from the same four samples using the three methods. ( B ) Electropherogram and Electrophoresis of representative sample of RNA analysed with the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits. The white arrow shows genomic DNA contaminants.
Figure Legend Snippet: Comparison of the effectiveness of RNAzol RT (RNA extraction in field) and TRIzol Reagent or spin-columns kit (RNA extraction in laboratory with recommended cold conditions) to isolate total RNA. ( A ) Electrophoretic separation using 2% agarose gel of RNA extracted from the same four samples using the three methods. ( B ) Electropherogram and Electrophoresis of representative sample of RNA analysed with the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits. The white arrow shows genomic DNA contaminants.

Techniques Used: RNA Extraction, Agarose Gel Electrophoresis, Electrophoresis

Depicts the integrity of RNA isolated at 30 °C from leaf tissue samples from Coffea arabica, Manihot esculenta, Oryza sativa and Zea mais . ( A ) The left-hand side of the figure shows the electrophoresis analysis by agarose gel. ( B ) The right-hand side of the figure shows the Agilent 2100 bioanalyser electrophoresis for each sample. The total RNA fraction had a RIN value ranging between 8 and 9.
Figure Legend Snippet: Depicts the integrity of RNA isolated at 30 °C from leaf tissue samples from Coffea arabica, Manihot esculenta, Oryza sativa and Zea mais . ( A ) The left-hand side of the figure shows the electrophoresis analysis by agarose gel. ( B ) The right-hand side of the figure shows the Agilent 2100 bioanalyser electrophoresis for each sample. The total RNA fraction had a RIN value ranging between 8 and 9.

Techniques Used: Isolation, Electrophoresis, Agarose Gel Electrophoresis

Related Articles

Electrophoresis:

Article Title: The Urinary Bladder Transcriptome and Proteome Defined by Transcriptomics and Antibody-Based Profiling
Article Snippet: .. RNA samples were analysed using either an Agilent 2100 Bioanalyser system (Agilent Biotechnologies, Palo Alto, USA) with the RNA 6000 Nano Labchip Kit or an Experion automated electrophoresis system (Bio-Rad Laboratories, Hercules, CA, USA) with the standard-sensitivity RNA chip. ..

Chromatin Immunoprecipitation:

Article Title: The Urinary Bladder Transcriptome and Proteome Defined by Transcriptomics and Antibody-Based Profiling
Article Snippet: .. RNA samples were analysed using either an Agilent 2100 Bioanalyser system (Agilent Biotechnologies, Palo Alto, USA) with the RNA 6000 Nano Labchip Kit or an Experion automated electrophoresis system (Bio-Rad Laboratories, Hercules, CA, USA) with the standard-sensitivity RNA chip. ..

Agarose Gel Electrophoresis:

Article Title: A single-step method for RNA isolation from tropical crops in the field
Article Snippet: .. A spectrophotometric analysis, an electrophoretic separation using agarose gel ( ) and the Agilent 2100 bioanalyser system ( ) show similar results (quality and quantity) when total RNA were isolated in field using RNAzol RT reagent or TRIzol reagent in the lab (without breaking the cold chain). .. The worst results were obtained with the spin-columns kit which is not well adapted to coffee leaves RNA extraction.

Spectrophotometry:

Article Title: Codon misreading tRNAs promote tumor growth in mice
Article Snippet: .. RNA quantity and integrity were assessed using the Nanodrop 1000 Spectrophotometer (Thermo Scientific) and Agilent 2100 Bioanalyser system, respectively. .. 25 mg of tumor tissue (H460- (n = 3), MKN74- (n = 3), and NIH3T3-derived tumors (n = 1)) were homogenized in Protein Lysis Buffer (0.5% Triton X-100, 50mM HEPES, 250mM NaCl, 1mM DTT, 1mM NaF, 2mM EDTA, 1mM EGTA, 1mM PMSF, 1mM Na3 VO4 supplemented with a cocktail of protease inhibitors (Complete, EDTA-free, Roche).

Isolation:

Article Title: A single-step method for RNA isolation from tropical crops in the field
Article Snippet: .. A spectrophotometric analysis, an electrophoretic separation using agarose gel ( ) and the Agilent 2100 bioanalyser system ( ) show similar results (quality and quantity) when total RNA were isolated in field using RNAzol RT reagent or TRIzol reagent in the lab (without breaking the cold chain). .. The worst results were obtained with the spin-columns kit which is not well adapted to coffee leaves RNA extraction.

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    Agilent technologies agilent 2100 bioanalyser
    Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Sections before LMD (a, c, e) , and sections after LM (b, d, f) . The area selected for laser microdissection is outlined in green (a region near the damage caused by larvae feeding, which comprised the stamen tissue, viewed at a , c and d ) or blue (a region farther from the injured area, which comprised the carpel tissue, viewed at a , e , and f ). The assessment of extracted RNA integrity from the stamen and carpel are shown in g and h , respectively. Electropherograms were obtained with an <t>Agilent</t> 2100 <t>Bioanalyser.</t> Open and closed arrowheads indicate the 18S and 28S ribosomal RNA peaks, respectively. RNA quality is expressed as the RNA integrity number (RIN). Scale bars = 100 μm.
    Agilent 2100 Bioanalyser, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 533 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent 2100 bioanalyser/product/Agilent technologies
    Average 94 stars, based on 533 article reviews
    Price from $9.99 to $1999.99
    agilent 2100 bioanalyser - by Bioz Stars, 2020-07
    94/100 stars
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    Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Sections before LMD (a, c, e) , and sections after LM (b, d, f) . The area selected for laser microdissection is outlined in green (a region near the damage caused by larvae feeding, which comprised the stamen tissue, viewed at a , c and d ) or blue (a region farther from the injured area, which comprised the carpel tissue, viewed at a , e , and f ). The assessment of extracted RNA integrity from the stamen and carpel are shown in g and h , respectively. Electropherograms were obtained with an Agilent 2100 Bioanalyser. Open and closed arrowheads indicate the 18S and 28S ribosomal RNA peaks, respectively. RNA quality is expressed as the RNA integrity number (RIN). Scale bars = 100 μm.

    Journal: BMC Genomics

    Article Title: Transcriptome analysis of Gossypium hirsutum flower buds infested by cotton boll weevil (Anthonomus grandis) larvae

    doi: 10.1186/1471-2164-15-854

    Figure Lengend Snippet: Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Sections before LMD (a, c, e) , and sections after LM (b, d, f) . The area selected for laser microdissection is outlined in green (a region near the damage caused by larvae feeding, which comprised the stamen tissue, viewed at a , c and d ) or blue (a region farther from the injured area, which comprised the carpel tissue, viewed at a , e , and f ). The assessment of extracted RNA integrity from the stamen and carpel are shown in g and h , respectively. Electropherograms were obtained with an Agilent 2100 Bioanalyser. Open and closed arrowheads indicate the 18S and 28S ribosomal RNA peaks, respectively. RNA quality is expressed as the RNA integrity number (RIN). Scale bars = 100 μm.

    Article Snippet: The quality of total RNA extracted from LMD-collected tissues was assessed using an RNA 6000 Pico kit on the Agilent 2100 Bioanalyser (Agilent Technologies).

    Techniques: Isolation, Laser Capture Microdissection

    Quality of root-extracted RNA. The analysis of the total RNA extracted from inoculated roots was performed using an Agilent Bioanalyser 2100. One microlitre of RQ1 DNase-treated total RNA was analysed for each sample. The days post-inoculation with  Gaeumannomyces graminis  var.  tritici  ( Ggt ) are indicated. M, RNA size marker; lanes 1, 3, 6, 9, RNA from uninfected wheat roots; lanes 2, 4, 7, 10, RNA from wheat roots infected with  Ggt ; lanes 5, 8, 11, RNA from wheat roots infected with  Ggt  and Pf29Arp; lane 12, RNA from soil-borne pathogenic fungus IV-26/00.

    Journal: Molecular Plant Pathology

    Article Title: The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis-related gene expression of the take-all fungus Gaeumannomyces graminis var. tritici on wheat roots

    doi: 10.1111/j.1364-3703.2011.00715.x

    Figure Lengend Snippet: Quality of root-extracted RNA. The analysis of the total RNA extracted from inoculated roots was performed using an Agilent Bioanalyser 2100. One microlitre of RQ1 DNase-treated total RNA was analysed for each sample. The days post-inoculation with Gaeumannomyces graminis var. tritici ( Ggt ) are indicated. M, RNA size marker; lanes 1, 3, 6, 9, RNA from uninfected wheat roots; lanes 2, 4, 7, 10, RNA from wheat roots infected with Ggt ; lanes 5, 8, 11, RNA from wheat roots infected with Ggt and Pf29Arp; lane 12, RNA from soil-borne pathogenic fungus IV-26/00.

    Article Snippet: RNA purity and quality were assessed with a Bioanalyser 2100 (Agilent) and quantified with a Nanodrop (Agilent).

    Techniques: Marker, Infection

    Murine CD4 + CD25 + Treg EVs contain different miRNA compared to control T cell EVs. ( A) dTreg and control FoxP3 - T cell EVs (n = 3 per group) isolated by ExoQuick-TC were lysed and total RNA was purified and assessed using NanoDrop™ spectrophotometer and Agilent 2100 Bioanalyser. EVs miRNA was reverse transcribed into cDNA and pre-amplified before miRNA detection using QuantStudio™ 12 K Flex Real-Time PCR System with the OpenArray® Platform. The volcano plot shows the relation between the p-value and the log fold change between FoxP3 − T cell and Treg EV miRNA expression levels. The blue line indicates the inverse log 10 of the p-value = 0.05. ( B ) Bar graphs (mean + SEM) showing the relative quantity of miR-142-3p, miR-150-5p, and miR-384-5p by control T cell EVs (Control EVs) and Treg EVs (Treg EVs) as measured by qPCR and normalised relative to RNU6-2. Data pooled from 3 individual experiments that were performed in triplicates. Statistical significance was determined using a two-tailed Student’s t-test where *p

    Journal: Scientific Reports

    Article Title: Regulatory T cell-derived extracellular vesicles modify dendritic cell function

    doi: 10.1038/s41598-018-24531-8

    Figure Lengend Snippet: Murine CD4 + CD25 + Treg EVs contain different miRNA compared to control T cell EVs. ( A) dTreg and control FoxP3 - T cell EVs (n = 3 per group) isolated by ExoQuick-TC were lysed and total RNA was purified and assessed using NanoDrop™ spectrophotometer and Agilent 2100 Bioanalyser. EVs miRNA was reverse transcribed into cDNA and pre-amplified before miRNA detection using QuantStudio™ 12 K Flex Real-Time PCR System with the OpenArray® Platform. The volcano plot shows the relation between the p-value and the log fold change between FoxP3 − T cell and Treg EV miRNA expression levels. The blue line indicates the inverse log 10 of the p-value = 0.05. ( B ) Bar graphs (mean + SEM) showing the relative quantity of miR-142-3p, miR-150-5p, and miR-384-5p by control T cell EVs (Control EVs) and Treg EVs (Treg EVs) as measured by qPCR and normalised relative to RNU6-2. Data pooled from 3 individual experiments that were performed in triplicates. Statistical significance was determined using a two-tailed Student’s t-test where *p

    Article Snippet: Total RNA profile was assessed using an Agilent 2100 Bioanalyser and total RNA nano chips and quantified by NanoDrop™ spectrophotometer (Thermo Fisher Scientific). cDNA fragments were synthesised using the ‘miScript RT Kit’ (Qiagen, Hilden, Germany), according to manufacturer’s protocols. qPCR reactions were set up with the miScript SYBR Green PCR Kit and primers specific for miR-125b-5p, miR-29a-3p, miR-182-5p, miR-142-3p, miR-150-5p, miR-384-5p, SCARNA17 and RNU6-2, as per manufacturer’s protocols and run on the Applied Biosystems™ ViiA™ 7 Real time PCR system.

    Techniques: Isolation, Purification, Spectrophotometry, Amplification, Real-time Polymerase Chain Reaction, Expressing, Two Tailed Test

    Capillary electrophoreses of IPCR (odd-numbered lanes) and PAI-PCR (even-numbered lanes) products amplified from DNA extracted from horse vermiform appendixes. The electrophoreses followed by the analysis of the electrophoregrams were automatically performed by using an Agilent 2100 Bioanalyser with a DNA 7500 LabChip® kit (TaKaRa-Bio). The results are shown as a gel-like image. L is the ladder obtained with DNA fragment size markers (bp). The green and pink peaks are the lowest 50 bp and highest 10 380 bp markers respectively. The substrates used for IPCR or PAI-PCR were BamHI-digested DNA (lanes 1 and 2), EcoRI-digested DNA (lanes 3 and 4), HindIII-digested DNA (lanes 5 and 6), PstI-digested DNA (lanes 7 and 8) and XbaI-digested DNA (lanes 9 and 10).

    Journal: Environmental Microbiology

    Article Title: Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids

    doi: 10.1111/j.1462-2920.2007.01518.x

    Figure Lengend Snippet: Capillary electrophoreses of IPCR (odd-numbered lanes) and PAI-PCR (even-numbered lanes) products amplified from DNA extracted from horse vermiform appendixes. The electrophoreses followed by the analysis of the electrophoregrams were automatically performed by using an Agilent 2100 Bioanalyser with a DNA 7500 LabChip® kit (TaKaRa-Bio). The results are shown as a gel-like image. L is the ladder obtained with DNA fragment size markers (bp). The green and pink peaks are the lowest 50 bp and highest 10 380 bp markers respectively. The substrates used for IPCR or PAI-PCR were BamHI-digested DNA (lanes 1 and 2), EcoRI-digested DNA (lanes 3 and 4), HindIII-digested DNA (lanes 5 and 6), PstI-digested DNA (lanes 7 and 8) and XbaI-digested DNA (lanes 9 and 10).

    Article Snippet: Products were analysed with an Agilent 2100 Bioanalyser with a DNA7500 LabChip® kit (TaKaRa-Bio).

    Techniques: Polymerase Chain Reaction, Amplification

    Capillary electrophoreses of PAI-PCR products amplified from DNA extracted from 12 different termite gut samples. All DNA samples were digested with EcoRI, and an RCA primer [(A) Xyn-RC(LNA-Even); or (B) Xyn-RC(LNA-5′)] was used in the RCA reaction. Products were analysed with an Agilent 2100 Bioanalyser with a DNA 7500 LabChip® kit (TaKaRa-Bio).

    Journal: Environmental Microbiology

    Article Title: Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids

    doi: 10.1111/j.1462-2920.2007.01518.x

    Figure Lengend Snippet: Capillary electrophoreses of PAI-PCR products amplified from DNA extracted from 12 different termite gut samples. All DNA samples were digested with EcoRI, and an RCA primer [(A) Xyn-RC(LNA-Even); or (B) Xyn-RC(LNA-5′)] was used in the RCA reaction. Products were analysed with an Agilent 2100 Bioanalyser with a DNA 7500 LabChip® kit (TaKaRa-Bio).

    Article Snippet: Products were analysed with an Agilent 2100 Bioanalyser with a DNA7500 LabChip® kit (TaKaRa-Bio).

    Techniques: Polymerase Chain Reaction, Amplification