quantitect sybr green pcr kit  (Qiagen)


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    Name:
    QuantiTect SYBR Green PCR Kit
    Description:
    For real time PCR and two step RT PCR using SYBR Green Kit contents Qiagen QuantiTect SYBR Green PCR Kit 40 x 50L rxns cDNA and DNA Sample Real time PCR and two step RT PCR Reaction With ROX Ideal for Real time Quantification of Genomic DNA or cDNA Targets For Real time PCR and Two step RT PCR Using SYBR Green Includes 1mL 2x QuantiTect SYBR Green PCR Master Mix 2mL RNase free Water Benefits High PCR specificity with integrated hot start Reliable quantification of low abundance transcripts Accurate quantification over several logs of template Available with or without uracil N glycosylase UNG No need to optimize reaction and cycling conditio
    Catalog Number:
    204141
    Price:
    127
    Category:
    QuantiTect SYBR Green PCR Kits
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    Structured Review

    Qiagen quantitect sybr green pcr kit
    QuantiTect SYBR Green PCR Kit
    For real time PCR and two step RT PCR using SYBR Green Kit contents Qiagen QuantiTect SYBR Green PCR Kit 40 x 50L rxns cDNA and DNA Sample Real time PCR and two step RT PCR Reaction With ROX Ideal for Real time Quantification of Genomic DNA or cDNA Targets For Real time PCR and Two step RT PCR Using SYBR Green Includes 1mL 2x QuantiTect SYBR Green PCR Master Mix 2mL RNase free Water Benefits High PCR specificity with integrated hot start Reliable quantification of low abundance transcripts Accurate quantification over several logs of template Available with or without uracil N glycosylase UNG No need to optimize reaction and cycling conditio
    https://www.bioz.com/result/quantitect sybr green pcr kit/product/Qiagen
    Average 90 stars, based on 1159 article reviews
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    quantitect sybr green pcr kit - by Bioz Stars, 2020-01
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    Related Articles

    Centrifugation:

    Article Title: Altered Ca2+ Regulation of Yop Secretion in Yersinia enterocolitica after DNA Adenine Methyltransferase Overproduction Is Mediated by Clp-Dependent Degradation of LcrG
    Article Snippet: Briefly, overnight cultures grown at 26°C were subcultivated in BHI in the presence of Ca2+ and 1 mM IPTG to induce DamOP from pTP166Kan at 26°C for 2 h and at 37°C for 2 h. The bacterial cells were collected by centrifugation and washed in 0.85% (wt/vol) NaCl before enzyme activity assays. .. Relative gene expression was determined by qRT-PCR with the Light-Cycler system (Roche) and the QuantiTect SYBR green PCR kit (QIAGEN) according to the manufacturers' manuals.

    Amplification:

    Article Title: Roles of Four Chitinases (ChiA, ChiB, ChiC, and ChiD) in the Chitin Degradation System of Marine Bacterium Alteromonas sp. Strain O-7
    Article Snippet: .. PCR amplification was monitored by use of a QuantiTect SYBR green PCR kit (QIAGEN) and a LightCycler instrument (Roche Diagnostics). .. Serial 10-fold dilutions of a known amount of plasmid carrying the target chitinase gene, which corresponded to 1.0 × 103 to 1.0 × 107 copies of each gene, were used as standard samples.

    Article Title: Targeted Gene Silencing in the Model Mushroom Coprinopsis cinerea (Coprinus cinereus) by Expression of Homologous Hairpin RNAs
    Article Snippet: Real-time PCR was carried out by using a QuantiTect SYBR Green PCR kit (QIAGEN) with 0.9 μM forward and reverse primer concentrations each and a variable amount of cDNA (20 to 0.02 ng per reaction) in a final reaction volume of 20 μl. .. Each run was completed with a melting curve analysis to confirm specificity of amplification and absence of primer dimers.

    Article Title: TREM-1-expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases
    Article Snippet: Human TREM-1 and mouse TREM-1 and TNF expression was also assessed by real-time PCR using the TREM-1 QuantiTect primer assay system and QuantiTect SYBR green PCR Kit (both from QIAGEN). .. DNA was amplified on a 7500 Real-Time PCR system (Applied Biosystems), and the increase in gene expression was calculated using Sequence Detection System software (Applied Biosystems).

    Article Title: Firefly luciferase gene contains a cryptic promoter
    Article Snippet: .. Two microliters of reverse transcriptase reaction were subjected to PCR amplification (15 min at 95°C; then 40 cycles of 30 sec at 94°C; 30 sec at 54°C; 1 min at 68°C; and finally, 4 min at 72°C) using a LightCycler 1.5 (Roche) and QuantiTect SYBR Green PCR kit (QIAGEN). ..

    Article Title: Expression pattern of neuronal and skeletal muscle voltage-gated Na+ channels in the developing mouse heart
    Article Snippet: Real-time RT-PCR was carried out using the LightCycler from Roche (Mannheim, Germany) in combination with the QuantiTect SYBR Green PCR Kit from Qiagen (Hilden, Germany). .. The following temperature profile was used for amplification: denaturation/activation for 1 cycle at 95°C for 15 min, and 45 cycles at 95°C for 1 min (temperature transition, 20°C s−1 ), 54–64°C for 1 min (temperature transition, 20°C s−1 ), and 72°C for 1–2 min (temperature transition, 3°C s−1 ) with fluorescence acquisition at 54–64°C in single mode.

    Article Title: Sustained Induction of NF-?B Is Required for Efficient Expression of Latent Human Immunodeficiency Virus Type 1 ▿
    Article Snippet: Amplification was performed with Taq polymerase (QIAGEN) for 35 to 40 cycles, and products were analyzed on 2.5% agarose gels. .. ChIP extracts were quantitated with the QuantiTect SYBR Green PCR kit (QIAGEN).

    Article Title: Matrix mineralization controls gene expression in osteoblastic cells
    Article Snippet: For QRT-PCR the QuantiTect SYBR Green PCR Kit (Qiagen) in combination with the StepOnePlus Real-Time PCR System (Applied Biosystems, Darmstadt, Germany) was employed. .. Each target was amplified in a total reaction volume of 20 μl per well, containing 10 μl QuantiTect SYBR Green PCR Master Mix, 2 μl QuantiTect Primer, 2 μl cDNA and 6 μl RNase-free water.

    Article Title: Mutation of the Plastidial ?-Glucan Phosphorylase Gene in Rice Affects the Synthesis and Structure of Starch in the Endosperm [W]
    Article Snippet: The extracts, including 5 μg total RNAs, were treated with 5 units of DNase I (Amplification Grade; Invitrogen) to completely remove contaminating genomic DNA. .. An aliquot of the first-strand cDNA mixture corresponding to 5 ng of total RNA served as the template for quantitative real-time RT-PCR analysis with the Quantitect SYBR Green PCR kit (Qiagen).

    Synthesized:

    Article Title: Firefly luciferase gene contains a cryptic promoter
    Article Snippet: 32 P-labeled antisense ssRNA hybridization probes displaying complementarity to luc+ CDS in regions 3–392 and 1411–1634 bp were synthesized using the T7 RNA transcription kit (Fermentas). .. Two microliters of reverse transcriptase reaction were subjected to PCR amplification (15 min at 95°C; then 40 cycles of 30 sec at 94°C; 30 sec at 54°C; 1 min at 68°C; and finally, 4 min at 72°C) using a LightCycler 1.5 (Roche) and QuantiTect SYBR Green PCR kit (QIAGEN).

    Article Title: Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control 1Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control 1 [W]
    Article Snippet: Two technical replicates were performed for each gene analyzed. cDNA was synthesized at 42°C for 1 h in 20 μ L reaction mixture using AMV (Roche) according to the manufacturer's protocol. .. Q-RT-PCR was performed in a 10- μ L reaction using a Quantitect SYBR Green PCR kit (Qiagen) in ABI PRISM 7700 sequence detection system (Applied Biosystems) under the following conditions: 95°C, 10 min; 95°C, 15 s; and 60°C, 1 min.

    Quantitative RT-PCR:

    Article Title: Altered Ca2+ Regulation of Yop Secretion in Yersinia enterocolitica after DNA Adenine Methyltransferase Overproduction Is Mediated by Clp-Dependent Degradation of LcrG
    Article Snippet: .. Relative gene expression was determined by qRT-PCR with the Light-Cycler system (Roche) and the QuantiTect SYBR green PCR kit (QIAGEN) according to the manufacturers' manuals. ..

    Article Title: Firefly luciferase gene contains a cryptic promoter
    Article Snippet: Paragraph title: RNA isolation, Northern blot, and real-time qRT PCR analysis ... Two microliters of reverse transcriptase reaction were subjected to PCR amplification (15 min at 95°C; then 40 cycles of 30 sec at 94°C; 30 sec at 54°C; 1 min at 68°C; and finally, 4 min at 72°C) using a LightCycler 1.5 (Roche) and QuantiTect SYBR Green PCR kit (QIAGEN).

    Article Title: Expression pattern of neuronal and skeletal muscle voltage-gated Na+ channels in the developing mouse heart
    Article Snippet: .. Real-time RT-PCR was carried out using the LightCycler from Roche (Mannheim, Germany) in combination with the QuantiTect SYBR Green PCR Kit from Qiagen (Hilden, Germany). ..

    Article Title: Matrix mineralization controls gene expression in osteoblastic cells
    Article Snippet: .. For QRT-PCR the QuantiTect SYBR Green PCR Kit (Qiagen) in combination with the StepOnePlus Real-Time PCR System (Applied Biosystems, Darmstadt, Germany) was employed. .. The following ten commercial primer sets QuantiTect Primer Assay (Qiagen) were used to detect targets of interest: HMCN1 (Hs_HMCN1_1_SG, Cat. no.: QT00011228), NID2 (Hs_NID2_1_SG, Cat. no.: QT00055258), ITGA2 (Hs_ITGA2_1_SG, Cat. no.: QT00086695), VCAN (Hs_VCAN_1_SG, Cat. no.: QT00064064), GAPDH (Hs_GAPDH_1_SG, Cat. no.: QT00079247) and ACTB (Hs_ACTB_1_SG, Cat. no.: QT00095431).

    Article Title: Mutation of the Plastidial ?-Glucan Phosphorylase Gene in Rice Affects the Synthesis and Structure of Starch in the Endosperm [W]
    Article Snippet: .. An aliquot of the first-strand cDNA mixture corresponding to 5 ng of total RNA served as the template for quantitative real-time RT-PCR analysis with the Quantitect SYBR Green PCR kit (Qiagen). .. Reactions were performed on an iCycler (Bio-Rad) according to the manufacturer's protocols.

    Real-time Polymerase Chain Reaction:

    Article Title: Roles of Four Chitinases (ChiA, ChiB, ChiC, and ChiD) in the Chitin Degradation System of Marine Bacterium Alteromonas sp. Strain O-7
    Article Snippet: Paragraph title: Real-time quantitative PCR analyses of chiA , -B , -C , and - D transcripts. ... PCR amplification was monitored by use of a QuantiTect SYBR green PCR kit (QIAGEN) and a LightCycler instrument (Roche Diagnostics).

    Article Title: MarA-Like Regulator of Multidrug Resistance in Yersinia pestis
    Article Snippet: .. The real-time PCR assay was carried out using the Quantitect SYBR Green PCR kit (QIAGEN). .. The primers either to acrAB Yp (SACABF-GCCGGTGATCGCCTGATTA and SACABR-ATGGTCGGATATTGCGCTAC), marA47 Yp (SA47F-CTATATCCGTGGGCGAGTGT and SA47R-GCTTGATTTCCGGCGTATAA), or YP16s (S16VF-CAGAAGAAGCACCGGCTAAC and S16VR-CGGGGATTTCACATCTGACT) rRNA were used for the real-time PCR carried out in the MX4000 multiplex quantitative PCR system from Stratagene.

    Article Title: Targeted Gene Silencing in the Model Mushroom Coprinopsis cinerea (Coprinus cinereus) by Expression of Homologous Hairpin RNAs
    Article Snippet: .. Real-time PCR was carried out by using a QuantiTect SYBR Green PCR kit (QIAGEN) with 0.9 μM forward and reverse primer concentrations each and a variable amount of cDNA (20 to 0.02 ng per reaction) in a final reaction volume of 20 μl. .. Thermocycling was performed by using a Rotor-Gene 3000 Real-Time Thermal Cycler (Corbett Research, Sydney, Australia) initiated by a 15-min incubation at 95°C, followed by 50 cycles of 15 s at 94°C, 30 s at 60°C, and 30 s at 72°C.

    Article Title: TREM-1-expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases
    Article Snippet: .. Human TREM-1 and mouse TREM-1 and TNF expression was also assessed by real-time PCR using the TREM-1 QuantiTect primer assay system and QuantiTect SYBR green PCR Kit (both from QIAGEN). .. GAPDH was used to normalize TREM-1 and TNF expression levels.

    Article Title: Matrix mineralization controls gene expression in osteoblastic cells
    Article Snippet: .. For QRT-PCR the QuantiTect SYBR Green PCR Kit (Qiagen) in combination with the StepOnePlus Real-Time PCR System (Applied Biosystems, Darmstadt, Germany) was employed. .. The following ten commercial primer sets QuantiTect Primer Assay (Qiagen) were used to detect targets of interest: HMCN1 (Hs_HMCN1_1_SG, Cat. no.: QT00011228), NID2 (Hs_NID2_1_SG, Cat. no.: QT00055258), ITGA2 (Hs_ITGA2_1_SG, Cat. no.: QT00086695), VCAN (Hs_VCAN_1_SG, Cat. no.: QT00064064), GAPDH (Hs_GAPDH_1_SG, Cat. no.: QT00079247) and ACTB (Hs_ACTB_1_SG, Cat. no.: QT00095431).

    Article Title: Mutation of the Plastidial ?-Glucan Phosphorylase Gene in Rice Affects the Synthesis and Structure of Starch in the Endosperm [W]
    Article Snippet: Transcript levels of Pho1 and Pho2 genes were determined by quantitative real-time PCR methods using oligonucleotide primer pairs and DNA amplification conditions as previously described by . .. An aliquot of the first-strand cDNA mixture corresponding to 5 ng of total RNA served as the template for quantitative real-time RT-PCR analysis with the Quantitect SYBR Green PCR kit (Qiagen).

    Article Title: Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster
    Article Snippet: .. Real-time PCR was conducted on a RotorGene-3000 (Corbett Research, Sydney) using a QuantiTect SYBR Green PCR kit (Qiagen, Valencia, CA). .. PCR conditions were 95°C for 10 min to activate the hot-start polymerase, followed by 50 cycles of 95°C for 20 sec, 55°C for 30 sec, and 72°C for 30 sec. Fluorescence was measured after each cycle.

    Article Title: Mutations in the Spike Protein of Middle East Respiratory Syndrome Coronavirus Transmitted in Korea Increase Resistance to Antibody-Mediated Neutralization
    Article Snippet: .. Subsequently, 1 μl of each sample was subjected to quantitative PCR (qPCR) using the QuantiTect SYBR green PCR kit (Qiagen) and a Rotor-Gene Q platform (Qiagen). .. Subsequently, 1 μl of each sample was subjected to quantitative PCR (qPCR) using the QuantiTect SYBR green PCR kit (Qiagen) and a Rotor-Gene Q platform (Qiagen).

    Microarray:

    Article Title: Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control 1Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control 1 [W]
    Article Snippet: The Q-RT-PCR was analyzed in three independent biological replicates, two of three replicates being the two samples used for microarray analysis. .. Q-RT-PCR was performed in a 10- μ L reaction using a Quantitect SYBR Green PCR kit (Qiagen) in ABI PRISM 7700 sequence detection system (Applied Biosystems) under the following conditions: 95°C, 10 min; 95°C, 15 s; and 60°C, 1 min.

    Incubation:

    Article Title: Targeted Gene Silencing in the Model Mushroom Coprinopsis cinerea (Coprinus cinereus) by Expression of Homologous Hairpin RNAs
    Article Snippet: Real-time PCR was carried out by using a QuantiTect SYBR Green PCR kit (QIAGEN) with 0.9 μM forward and reverse primer concentrations each and a variable amount of cDNA (20 to 0.02 ng per reaction) in a final reaction volume of 20 μl. .. Thermocycling was performed by using a Rotor-Gene 3000 Real-Time Thermal Cycler (Corbett Research, Sydney, Australia) initiated by a 15-min incubation at 95°C, followed by 50 cycles of 15 s at 94°C, 30 s at 60°C, and 30 s at 72°C.

    Article Title: Matrix mineralization controls gene expression in osteoblastic cells
    Article Snippet: For QRT-PCR the QuantiTect SYBR Green PCR Kit (Qiagen) in combination with the StepOnePlus Real-Time PCR System (Applied Biosystems, Darmstadt, Germany) was employed. .. DNA amplification was initiated through an incubation step of 15 minutes at 95°C, followed by 40 cycles of a 15 s denaturation step at 95 °C, a 30 seconds annealing step at 55 °C and a 30 seconds extension step at 72 °C.

    Activity Assay:

    Article Title: Altered Ca2+ Regulation of Yop Secretion in Yersinia enterocolitica after DNA Adenine Methyltransferase Overproduction Is Mediated by Clp-Dependent Degradation of LcrG
    Article Snippet: Briefly, overnight cultures grown at 26°C were subcultivated in BHI in the presence of Ca2+ and 1 mM IPTG to induce DamOP from pTP166Kan at 26°C for 2 h and at 37°C for 2 h. The bacterial cells were collected by centrifugation and washed in 0.85% (wt/vol) NaCl before enzyme activity assays. .. Relative gene expression was determined by qRT-PCR with the Light-Cycler system (Roche) and the QuantiTect SYBR green PCR kit (QIAGEN) according to the manufacturers' manuals.

    Expressing:

    Article Title: TREM-1-expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases
    Article Snippet: .. Human TREM-1 and mouse TREM-1 and TNF expression was also assessed by real-time PCR using the TREM-1 QuantiTect primer assay system and QuantiTect SYBR green PCR Kit (both from QIAGEN). .. GAPDH was used to normalize TREM-1 and TNF expression levels.

    Article Title: Altered Ca2+ Regulation of Yop Secretion in Yersinia enterocolitica after DNA Adenine Methyltransferase Overproduction Is Mediated by Clp-Dependent Degradation of LcrG
    Article Snippet: .. Relative gene expression was determined by qRT-PCR with the Light-Cycler system (Roche) and the QuantiTect SYBR green PCR kit (QIAGEN) according to the manufacturers' manuals. ..

    Article Title: Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control 1Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control 1 [W]
    Article Snippet: Q-RT-PCR was performed in a 10- μ L reaction using a Quantitect SYBR Green PCR kit (Qiagen) in ABI PRISM 7700 sequence detection system (Applied Biosystems) under the following conditions: 95°C, 10 min; 95°C, 15 s; and 60°C, 1 min. .. Five genes ( ) with a constitutive expression were used for data normalization.

    Article Title: Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster
    Article Snippet: Real-time PCR was conducted on a RotorGene-3000 (Corbett Research, Sydney) using a QuantiTect SYBR Green PCR kit (Qiagen, Valencia, CA). .. Relative expression of each gene was measured in reference to the housekeeping gene RpL11 .

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster
    Article Snippet: Real-time PCR was conducted on a RotorGene-3000 (Corbett Research, Sydney) using a QuantiTect SYBR Green PCR kit (Qiagen, Valencia, CA). .. PCR primers used were RpL11F (CGA TCC CTC CAT CGG TAT CT) and RpL11R (AAC CAC TTC ATG GCA TCC TC) for RPL11 , Cyp12a4F (CCA ATC GTC CAG GCA ACT AT) and Cyp12a4R (TCG GGA TCT CTC AGT TCG AG) for Cyp12a4 , and Cyp12a5F (ATC CTG GGG AGA CTT TCG AT) and Cyp12a5R (GCG GTT AAT GGT TTC CAA GA) for Cyp12a5 .

    Hybridization:

    Article Title: Firefly luciferase gene contains a cryptic promoter
    Article Snippet: Hybridization was carried out in Dig Easy Hyb solution according to Roche's instructions. .. Two microliters of reverse transcriptase reaction were subjected to PCR amplification (15 min at 95°C; then 40 cycles of 30 sec at 94°C; 30 sec at 54°C; 1 min at 68°C; and finally, 4 min at 72°C) using a LightCycler 1.5 (Roche) and QuantiTect SYBR Green PCR kit (QIAGEN).

    Conjugation Assay:

    Article Title: Altered Ca2+ Regulation of Yop Secretion in Yersinia enterocolitica after DNA Adenine Methyltransferase Overproduction Is Mediated by Clp-Dependent Degradation of LcrG
    Article Snippet: After conjugation of the plasmids to Y. enterocolitica JB580v from E. coli S17-1λ pir and integration into the virulence plasmid, merodiploid lcrG-lacZYA/lcrG + , yopN-lacZYA/yopN + , yscN-lacZYA/yscN + , orf76-lacZYA/orf76 + , and orf83-lacZYA/orf83 + strains were generated. .. Relative gene expression was determined by qRT-PCR with the Light-Cycler system (Roche) and the QuantiTect SYBR green PCR kit (QIAGEN) according to the manufacturers' manuals.

    Sequencing:

    Article Title: Neuropathy target esterase catalyzes osmoprotective renal synthesis of glycerophosphocholine in response to high NaCl
    Article Snippet: QiaShredder column, RNeasy mini kit, RNase-free DNase, and Quantitect SYBR Green PCR kit were from Qiagen (Valencia, CA). .. ABI Prism 7900 sequence detection system and TaqMan reverse-transcription reagents kit were from Applied Biosystems (Foster City, CA).

    Article Title: TREM-1-expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases
    Article Snippet: Human TREM-1 and mouse TREM-1 and TNF expression was also assessed by real-time PCR using the TREM-1 QuantiTect primer assay system and QuantiTect SYBR green PCR Kit (both from QIAGEN). .. DNA was amplified on a 7500 Real-Time PCR system (Applied Biosystems), and the increase in gene expression was calculated using Sequence Detection System software (Applied Biosystems).

    Article Title: Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control 1Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control 1 [W]
    Article Snippet: .. Q-RT-PCR was performed in a 10- μ L reaction using a Quantitect SYBR Green PCR kit (Qiagen) in ABI PRISM 7700 sequence detection system (Applied Biosystems) under the following conditions: 95°C, 10 min; 95°C, 15 s; and 60°C, 1 min. .. Five genes ( ) with a constitutive expression were used for data normalization.

    Southern Blot:

    Article Title: Altered Ca2+ Regulation of Yop Secretion in Yersinia enterocolitica after DNA Adenine Methyltransferase Overproduction Is Mediated by Clp-Dependent Degradation of LcrG
    Article Snippet: Proper integration of the plasmids into the Y. enterocolitica virulence plasmid was confirmed by Southern blot analysis (data not shown). β-Galactosidase assays were performed as previously described ( ). .. Relative gene expression was determined by qRT-PCR with the Light-Cycler system (Roche) and the QuantiTect SYBR green PCR kit (QIAGEN) according to the manufacturers' manuals.

    Northern Blot:

    Article Title: Firefly luciferase gene contains a cryptic promoter
    Article Snippet: Paragraph title: RNA isolation, Northern blot, and real-time qRT PCR analysis ... Two microliters of reverse transcriptase reaction were subjected to PCR amplification (15 min at 95°C; then 40 cycles of 30 sec at 94°C; 30 sec at 54°C; 1 min at 68°C; and finally, 4 min at 72°C) using a LightCycler 1.5 (Roche) and QuantiTect SYBR Green PCR kit (QIAGEN).

    Generated:

    Article Title: Altered Ca2+ Regulation of Yop Secretion in Yersinia enterocolitica after DNA Adenine Methyltransferase Overproduction Is Mediated by Clp-Dependent Degradation of LcrG
    Article Snippet: For the quantification of gene expression by qRT-PCR, cultures were grown in the presence of Ca2+ and 1 mM IPTG to induce DamOP from pTP166Kan at 26°C for 2 h and shifted to 37°C for 2 h. Total RNA was isolated with the RNeasy mini kit (QIAGEN). cDNA was generated by randomly primed reverse transcription (Revert Aid kit; Fermentas) after removal of contaminating DNA (Turbo DNA-free kit; Ambion). .. Relative gene expression was determined by qRT-PCR with the Light-Cycler system (Roche) and the QuantiTect SYBR green PCR kit (QIAGEN) according to the manufacturers' manuals.

    Imaging:

    Article Title: Sustained Induction of NF-?B Is Required for Efficient Expression of Latent Human Immunodeficiency Virus Type 1 ▿
    Article Snippet: Images were acquired using an EagleEye II digital imaging system (Stratagene). .. ChIP extracts were quantitated with the QuantiTect SYBR Green PCR kit (QIAGEN).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: TREM-1-expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases
    Article Snippet: Paragraph title: RNA isolation and RT-PCR. ... Human TREM-1 and mouse TREM-1 and TNF expression was also assessed by real-time PCR using the TREM-1 QuantiTect primer assay system and QuantiTect SYBR green PCR Kit (both from QIAGEN).

    Article Title: Expression pattern of neuronal and skeletal muscle voltage-gated Na+ channels in the developing mouse heart
    Article Snippet: Paragraph title: Transcript quantification with LightCycler RT-PCR (real-time RT-PCR) ... Real-time RT-PCR was carried out using the LightCycler from Roche (Mannheim, Germany) in combination with the QuantiTect SYBR Green PCR Kit from Qiagen (Hilden, Germany).

    Binding Assay:

    Article Title: Sustained Induction of NF-?B Is Required for Efficient Expression of Latent Human Immunodeficiency Virus Type 1 ▿
    Article Snippet: Specific enrichment in target DNA sequences by a specified antibody was interpreted as DNA binding by the antibody target. .. ChIP extracts were quantitated with the QuantiTect SYBR Green PCR kit (QIAGEN).

    Cellular Antioxidant Activity Assay:

    Article Title: Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster
    Article Snippet: Real-time PCR was conducted on a RotorGene-3000 (Corbett Research, Sydney) using a QuantiTect SYBR Green PCR kit (Qiagen, Valencia, CA). .. PCR primers used were RpL11F (CGA TCC CTC CAT CGG TAT CT) and RpL11R (AAC CAC TTC ATG GCA TCC TC) for RPL11 , Cyp12a4F (CCA ATC GTC CAG GCA ACT AT) and Cyp12a4R (TCG GGA TCT CTC AGT TCG AG) for Cyp12a4 , and Cyp12a5F (ATC CTG GGG AGA CTT TCG AT) and Cyp12a5R (GCG GTT AAT GGT TTC CAA GA) for Cyp12a5 .

    Fluorescence:

    Article Title: Targeted Gene Silencing in the Model Mushroom Coprinopsis cinerea (Coprinus cinereus) by Expression of Homologous Hairpin RNAs
    Article Snippet: Real-time PCR was carried out by using a QuantiTect SYBR Green PCR kit (QIAGEN) with 0.9 μM forward and reverse primer concentrations each and a variable amount of cDNA (20 to 0.02 ng per reaction) in a final reaction volume of 20 μl. .. Fluorescence data were acquired during the elongation step in every cycle.

    Article Title: Expression pattern of neuronal and skeletal muscle voltage-gated Na+ channels in the developing mouse heart
    Article Snippet: Real-time RT-PCR was carried out using the LightCycler from Roche (Mannheim, Germany) in combination with the QuantiTect SYBR Green PCR Kit from Qiagen (Hilden, Germany). .. The following temperature profile was used for amplification: denaturation/activation for 1 cycle at 95°C for 15 min, and 45 cycles at 95°C for 1 min (temperature transition, 20°C s−1 ), 54–64°C for 1 min (temperature transition, 20°C s−1 ), and 72°C for 1–2 min (temperature transition, 3°C s−1 ) with fluorescence acquisition at 54–64°C in single mode.

    Article Title: Sustained Induction of NF-?B Is Required for Efficient Expression of Latent Human Immunodeficiency Virus Type 1 ▿
    Article Snippet: ChIP extracts were quantitated with the QuantiTect SYBR Green PCR kit (QIAGEN). .. Fluorescence profiles were collected on an ABI 7700 real-time thermal cycler and analyzed with SDS v1.91 (Applied Biosystems).

    Article Title: Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster
    Article Snippet: Real-time PCR was conducted on a RotorGene-3000 (Corbett Research, Sydney) using a QuantiTect SYBR Green PCR kit (Qiagen, Valencia, CA). .. PCR conditions were 95°C for 10 min to activate the hot-start polymerase, followed by 50 cycles of 95°C for 20 sec, 55°C for 30 sec, and 72°C for 30 sec. Fluorescence was measured after each cycle.

    Mutagenesis:

    Article Title: Mutation of the Plastidial ?-Glucan Phosphorylase Gene in Rice Affects the Synthesis and Structure of Starch in the Endosperm [W]
    Article Snippet: Total RNAs from seeds of cv T65 and its pho1 mutant BMF65 were extracted with EASYPrep RNA (TaKaRa) according to the manufacturer's instructions. .. An aliquot of the first-strand cDNA mixture corresponding to 5 ng of total RNA served as the template for quantitative real-time RT-PCR analysis with the Quantitect SYBR Green PCR kit (Qiagen).

    Isolation:

    Article Title: Roles of Four Chitinases (ChiA, ChiB, ChiC, and ChiD) in the Chitin Degradation System of Marine Bacterium Alteromonas sp. Strain O-7
    Article Snippet: The total RNA was extracted from 1.0 ml of a cell suspension of Alteromonas sp. strain O-7 by use of an SV total RNA isolation system (Promega) according to the manufacturer's instructions. .. PCR amplification was monitored by use of a QuantiTect SYBR green PCR kit (QIAGEN) and a LightCycler instrument (Roche Diagnostics).

    Article Title: TREM-1-expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases
    Article Snippet: Paragraph title: RNA isolation and RT-PCR. ... Human TREM-1 and mouse TREM-1 and TNF expression was also assessed by real-time PCR using the TREM-1 QuantiTect primer assay system and QuantiTect SYBR green PCR Kit (both from QIAGEN).

    Article Title: Altered Ca2+ Regulation of Yop Secretion in Yersinia enterocolitica after DNA Adenine Methyltransferase Overproduction Is Mediated by Clp-Dependent Degradation of LcrG
    Article Snippet: For the quantification of gene expression by qRT-PCR, cultures were grown in the presence of Ca2+ and 1 mM IPTG to induce DamOP from pTP166Kan at 26°C for 2 h and shifted to 37°C for 2 h. Total RNA was isolated with the RNeasy mini kit (QIAGEN). cDNA was generated by randomly primed reverse transcription (Revert Aid kit; Fermentas) after removal of contaminating DNA (Turbo DNA-free kit; Ambion). .. Relative gene expression was determined by qRT-PCR with the Light-Cycler system (Roche) and the QuantiTect SYBR green PCR kit (QIAGEN) according to the manufacturers' manuals.

    Article Title: Firefly luciferase gene contains a cryptic promoter
    Article Snippet: Paragraph title: RNA isolation, Northern blot, and real-time qRT PCR analysis ... Two microliters of reverse transcriptase reaction were subjected to PCR amplification (15 min at 95°C; then 40 cycles of 30 sec at 94°C; 30 sec at 54°C; 1 min at 68°C; and finally, 4 min at 72°C) using a LightCycler 1.5 (Roche) and QuantiTect SYBR Green PCR kit (QIAGEN).

    Size-exclusion Chromatography:

    Article Title: Firefly luciferase gene contains a cryptic promoter
    Article Snippet: .. Two microliters of reverse transcriptase reaction were subjected to PCR amplification (15 min at 95°C; then 40 cycles of 30 sec at 94°C; 30 sec at 54°C; 1 min at 68°C; and finally, 4 min at 72°C) using a LightCycler 1.5 (Roche) and QuantiTect SYBR Green PCR kit (QIAGEN). ..

    Article Title: Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster
    Article Snippet: Real-time PCR was conducted on a RotorGene-3000 (Corbett Research, Sydney) using a QuantiTect SYBR Green PCR kit (Qiagen, Valencia, CA). .. PCR conditions were 95°C for 10 min to activate the hot-start polymerase, followed by 50 cycles of 95°C for 20 sec, 55°C for 30 sec, and 72°C for 30 sec. Fluorescence was measured after each cycle.

    Polymerase Chain Reaction:

    Article Title: Roles of Four Chitinases (ChiA, ChiB, ChiC, and ChiD) in the Chitin Degradation System of Marine Bacterium Alteromonas sp. Strain O-7
    Article Snippet: .. PCR amplification was monitored by use of a QuantiTect SYBR green PCR kit (QIAGEN) and a LightCycler instrument (Roche Diagnostics). .. Serial 10-fold dilutions of a known amount of plasmid carrying the target chitinase gene, which corresponded to 1.0 × 103 to 1.0 × 107 copies of each gene, were used as standard samples.

    Article Title: Neuropathy target esterase catalyzes osmoprotective renal synthesis of glycerophosphocholine in response to high NaCl
    Article Snippet: .. QiaShredder column, RNeasy mini kit, RNase-free DNase, and Quantitect SYBR Green PCR kit were from Qiagen (Valencia, CA). .. Lipofectamine 2000 was from Invitrogen (Carlsbad, CA).

    Article Title: MarA-Like Regulator of Multidrug Resistance in Yersinia pestis
    Article Snippet: .. The real-time PCR assay was carried out using the Quantitect SYBR Green PCR kit (QIAGEN). .. The primers either to acrAB Yp (SACABF-GCCGGTGATCGCCTGATTA and SACABR-ATGGTCGGATATTGCGCTAC), marA47 Yp (SA47F-CTATATCCGTGGGCGAGTGT and SA47R-GCTTGATTTCCGGCGTATAA), or YP16s (S16VF-CAGAAGAAGCACCGGCTAAC and S16VR-CGGGGATTTCACATCTGACT) rRNA were used for the real-time PCR carried out in the MX4000 multiplex quantitative PCR system from Stratagene.

    Article Title: Targeted Gene Silencing in the Model Mushroom Coprinopsis cinerea (Coprinus cinereus) by Expression of Homologous Hairpin RNAs
    Article Snippet: .. Real-time PCR was carried out by using a QuantiTect SYBR Green PCR kit (QIAGEN) with 0.9 μM forward and reverse primer concentrations each and a variable amount of cDNA (20 to 0.02 ng per reaction) in a final reaction volume of 20 μl. .. Thermocycling was performed by using a Rotor-Gene 3000 Real-Time Thermal Cycler (Corbett Research, Sydney, Australia) initiated by a 15-min incubation at 95°C, followed by 50 cycles of 15 s at 94°C, 30 s at 60°C, and 30 s at 72°C.

    Article Title: TREM-1-expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases
    Article Snippet: .. Human TREM-1 and mouse TREM-1 and TNF expression was also assessed by real-time PCR using the TREM-1 QuantiTect primer assay system and QuantiTect SYBR green PCR Kit (both from QIAGEN). .. GAPDH was used to normalize TREM-1 and TNF expression levels.

    Article Title: Altered Ca2+ Regulation of Yop Secretion in Yersinia enterocolitica after DNA Adenine Methyltransferase Overproduction Is Mediated by Clp-Dependent Degradation of LcrG
    Article Snippet: .. Relative gene expression was determined by qRT-PCR with the Light-Cycler system (Roche) and the QuantiTect SYBR green PCR kit (QIAGEN) according to the manufacturers' manuals. ..

    Article Title: Firefly luciferase gene contains a cryptic promoter
    Article Snippet: .. Two microliters of reverse transcriptase reaction were subjected to PCR amplification (15 min at 95°C; then 40 cycles of 30 sec at 94°C; 30 sec at 54°C; 1 min at 68°C; and finally, 4 min at 72°C) using a LightCycler 1.5 (Roche) and QuantiTect SYBR Green PCR kit (QIAGEN). ..

    Article Title: Expression pattern of neuronal and skeletal muscle voltage-gated Na+ channels in the developing mouse heart
    Article Snippet: .. Real-time RT-PCR was carried out using the LightCycler from Roche (Mannheim, Germany) in combination with the QuantiTect SYBR Green PCR Kit from Qiagen (Hilden, Germany). ..

    Article Title: Sustained Induction of NF-?B Is Required for Efficient Expression of Latent Human Immunodeficiency Virus Type 1 ▿
    Article Snippet: .. ChIP extracts were quantitated with the QuantiTect SYBR Green PCR kit (QIAGEN). .. Fluorescence profiles were collected on an ABI 7700 real-time thermal cycler and analyzed with SDS v1.91 (Applied Biosystems).

    Article Title: Matrix mineralization controls gene expression in osteoblastic cells
    Article Snippet: .. For QRT-PCR the QuantiTect SYBR Green PCR Kit (Qiagen) in combination with the StepOnePlus Real-Time PCR System (Applied Biosystems, Darmstadt, Germany) was employed. .. The following ten commercial primer sets QuantiTect Primer Assay (Qiagen) were used to detect targets of interest: HMCN1 (Hs_HMCN1_1_SG, Cat. no.: QT00011228), NID2 (Hs_NID2_1_SG, Cat. no.: QT00055258), ITGA2 (Hs_ITGA2_1_SG, Cat. no.: QT00086695), VCAN (Hs_VCAN_1_SG, Cat. no.: QT00064064), GAPDH (Hs_GAPDH_1_SG, Cat. no.: QT00079247) and ACTB (Hs_ACTB_1_SG, Cat. no.: QT00095431).

    Article Title: Mutation of the Plastidial ?-Glucan Phosphorylase Gene in Rice Affects the Synthesis and Structure of Starch in the Endosperm [W]
    Article Snippet: .. An aliquot of the first-strand cDNA mixture corresponding to 5 ng of total RNA served as the template for quantitative real-time RT-PCR analysis with the Quantitect SYBR Green PCR kit (Qiagen). .. Reactions were performed on an iCycler (Bio-Rad) according to the manufacturer's protocols.

    Article Title: Translational regulation of glutathione peroxidase 4 expression through guanine-rich sequence-binding factor 1 is essential for embryonic brain development
    Article Snippet: .. The chemicals used were from the following sources: SuperScript III reverse transcriptase and RNaseOUT from Invitrogen; BD Advantage 2 Polymerase Mix from BD Biosciences (Pharmingen); dNTPs from Carl Roth GmbH; QuantiTect SYBR Green PCR Kit from Qiagen; and PCR primers from BIOTEZ. .. The yeast strains YBZ-1 and R40coat as well as the plasmid pIIIA/MS2-1 were a generous gift from D. Bernstein (University Wisconsin–Madison, Madison).

    Article Title: Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control 1Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control 1 [W]
    Article Snippet: .. Q-RT-PCR was performed in a 10- μ L reaction using a Quantitect SYBR Green PCR kit (Qiagen) in ABI PRISM 7700 sequence detection system (Applied Biosystems) under the following conditions: 95°C, 10 min; 95°C, 15 s; and 60°C, 1 min. .. Five genes ( ) with a constitutive expression were used for data normalization.

    Article Title: Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster
    Article Snippet: .. Real-time PCR was conducted on a RotorGene-3000 (Corbett Research, Sydney) using a QuantiTect SYBR Green PCR kit (Qiagen, Valencia, CA). .. PCR conditions were 95°C for 10 min to activate the hot-start polymerase, followed by 50 cycles of 95°C for 20 sec, 55°C for 30 sec, and 72°C for 30 sec. Fluorescence was measured after each cycle.

    Article Title: Mutations in the Spike Protein of Middle East Respiratory Syndrome Coronavirus Transmitted in Korea Increase Resistance to Antibody-Mediated Neutralization
    Article Snippet: .. Subsequently, 1 μl of each sample was subjected to quantitative PCR (qPCR) using the QuantiTect SYBR green PCR kit (Qiagen) and a Rotor-Gene Q platform (Qiagen). .. Subsequently, 1 μl of each sample was subjected to quantitative PCR (qPCR) using the QuantiTect SYBR green PCR kit (Qiagen) and a Rotor-Gene Q platform (Qiagen).

    Protein Extraction:

    Article Title: Neuropathy target esterase catalyzes osmoprotective renal synthesis of glycerophosphocholine in response to high NaCl
    Article Snippet: QiaShredder column, RNeasy mini kit, RNase-free DNase, and Quantitect SYBR Green PCR kit were from Qiagen (Valencia, CA). .. Mammalian protein extraction reagent was from Pierce (Rockford, IL), and complete miniprotease inhibitor tablets were from Roche (Indianapolis, IN).

    Activated Clotting Time Assay:

    Article Title: Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster
    Article Snippet: Real-time PCR was conducted on a RotorGene-3000 (Corbett Research, Sydney) using a QuantiTect SYBR Green PCR kit (Qiagen, Valencia, CA). .. PCR primers used were RpL11F (CGA TCC CTC CAT CGG TAT CT) and RpL11R (AAC CAC TTC ATG GCA TCC TC) for RPL11 , Cyp12a4F (CCA ATC GTC CAG GCA ACT AT) and Cyp12a4R (TCG GGA TCT CTC AGT TCG AG) for Cyp12a4 , and Cyp12a5F (ATC CTG GGG AGA CTT TCG AT) and Cyp12a5R (GCG GTT AAT GGT TTC CAA GA) for Cyp12a5 .

    Chromatin Immunoprecipitation:

    Article Title: Sustained Induction of NF-?B Is Required for Efficient Expression of Latent Human Immunodeficiency Virus Type 1 ▿
    Article Snippet: .. ChIP extracts were quantitated with the QuantiTect SYBR Green PCR kit (QIAGEN). .. Fluorescence profiles were collected on an ABI 7700 real-time thermal cycler and analyzed with SDS v1.91 (Applied Biosystems).

    Plasmid Preparation:

    Article Title: Roles of Four Chitinases (ChiA, ChiB, ChiC, and ChiD) in the Chitin Degradation System of Marine Bacterium Alteromonas sp. Strain O-7
    Article Snippet: PCR amplification was monitored by use of a QuantiTect SYBR green PCR kit (QIAGEN) and a LightCycler instrument (Roche Diagnostics). .. Serial 10-fold dilutions of a known amount of plasmid carrying the target chitinase gene, which corresponded to 1.0 × 103 to 1.0 × 107 copies of each gene, were used as standard samples.

    Article Title: Altered Ca2+ Regulation of Yop Secretion in Yersinia enterocolitica after DNA Adenine Methyltransferase Overproduction Is Mediated by Clp-Dependent Degradation of LcrG
    Article Snippet: Proper integration of the plasmids into the Y. enterocolitica virulence plasmid was confirmed by Southern blot analysis (data not shown). β-Galactosidase assays were performed as previously described ( ). .. Relative gene expression was determined by qRT-PCR with the Light-Cycler system (Roche) and the QuantiTect SYBR green PCR kit (QIAGEN) according to the manufacturers' manuals.

    Article Title: Translational regulation of glutathione peroxidase 4 expression through guanine-rich sequence-binding factor 1 is essential for embryonic brain development
    Article Snippet: The chemicals used were from the following sources: SuperScript III reverse transcriptase and RNaseOUT from Invitrogen; BD Advantage 2 Polymerase Mix from BD Biosciences (Pharmingen); dNTPs from Carl Roth GmbH; QuantiTect SYBR Green PCR Kit from Qiagen; and PCR primers from BIOTEZ. .. The yeast strains YBZ-1 and R40coat as well as the plasmid pIIIA/MS2-1 were a generous gift from D. Bernstein (University Wisconsin–Madison, Madison).

    Software:

    Article Title: TREM-1-expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases
    Article Snippet: Human TREM-1 and mouse TREM-1 and TNF expression was also assessed by real-time PCR using the TREM-1 QuantiTect primer assay system and QuantiTect SYBR green PCR Kit (both from QIAGEN). .. DNA was amplified on a 7500 Real-Time PCR system (Applied Biosystems), and the increase in gene expression was calculated using Sequence Detection System software (Applied Biosystems).

    SYBR Green Assay:

    Article Title: Roles of Four Chitinases (ChiA, ChiB, ChiC, and ChiD) in the Chitin Degradation System of Marine Bacterium Alteromonas sp. Strain O-7
    Article Snippet: .. PCR amplification was monitored by use of a QuantiTect SYBR green PCR kit (QIAGEN) and a LightCycler instrument (Roche Diagnostics). .. Serial 10-fold dilutions of a known amount of plasmid carrying the target chitinase gene, which corresponded to 1.0 × 103 to 1.0 × 107 copies of each gene, were used as standard samples.

    Article Title: Neuropathy target esterase catalyzes osmoprotective renal synthesis of glycerophosphocholine in response to high NaCl
    Article Snippet: .. QiaShredder column, RNeasy mini kit, RNase-free DNase, and Quantitect SYBR Green PCR kit were from Qiagen (Valencia, CA). .. Lipofectamine 2000 was from Invitrogen (Carlsbad, CA).

    Article Title: MarA-Like Regulator of Multidrug Resistance in Yersinia pestis
    Article Snippet: .. The real-time PCR assay was carried out using the Quantitect SYBR Green PCR kit (QIAGEN). .. The primers either to acrAB Yp (SACABF-GCCGGTGATCGCCTGATTA and SACABR-ATGGTCGGATATTGCGCTAC), marA47 Yp (SA47F-CTATATCCGTGGGCGAGTGT and SA47R-GCTTGATTTCCGGCGTATAA), or YP16s (S16VF-CAGAAGAAGCACCGGCTAAC and S16VR-CGGGGATTTCACATCTGACT) rRNA were used for the real-time PCR carried out in the MX4000 multiplex quantitative PCR system from Stratagene.

    Article Title: Targeted Gene Silencing in the Model Mushroom Coprinopsis cinerea (Coprinus cinereus) by Expression of Homologous Hairpin RNAs
    Article Snippet: .. Real-time PCR was carried out by using a QuantiTect SYBR Green PCR kit (QIAGEN) with 0.9 μM forward and reverse primer concentrations each and a variable amount of cDNA (20 to 0.02 ng per reaction) in a final reaction volume of 20 μl. .. Thermocycling was performed by using a Rotor-Gene 3000 Real-Time Thermal Cycler (Corbett Research, Sydney, Australia) initiated by a 15-min incubation at 95°C, followed by 50 cycles of 15 s at 94°C, 30 s at 60°C, and 30 s at 72°C.

    Article Title: TREM-1-expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases
    Article Snippet: .. Human TREM-1 and mouse TREM-1 and TNF expression was also assessed by real-time PCR using the TREM-1 QuantiTect primer assay system and QuantiTect SYBR green PCR Kit (both from QIAGEN). .. GAPDH was used to normalize TREM-1 and TNF expression levels.

    Article Title: Altered Ca2+ Regulation of Yop Secretion in Yersinia enterocolitica after DNA Adenine Methyltransferase Overproduction Is Mediated by Clp-Dependent Degradation of LcrG
    Article Snippet: .. Relative gene expression was determined by qRT-PCR with the Light-Cycler system (Roche) and the QuantiTect SYBR green PCR kit (QIAGEN) according to the manufacturers' manuals. ..

    Article Title: Firefly luciferase gene contains a cryptic promoter
    Article Snippet: .. Two microliters of reverse transcriptase reaction were subjected to PCR amplification (15 min at 95°C; then 40 cycles of 30 sec at 94°C; 30 sec at 54°C; 1 min at 68°C; and finally, 4 min at 72°C) using a LightCycler 1.5 (Roche) and QuantiTect SYBR Green PCR kit (QIAGEN). ..

    Article Title: Expression pattern of neuronal and skeletal muscle voltage-gated Na+ channels in the developing mouse heart
    Article Snippet: .. Real-time RT-PCR was carried out using the LightCycler from Roche (Mannheim, Germany) in combination with the QuantiTect SYBR Green PCR Kit from Qiagen (Hilden, Germany). ..

    Article Title: Sustained Induction of NF-?B Is Required for Efficient Expression of Latent Human Immunodeficiency Virus Type 1 ▿
    Article Snippet: .. ChIP extracts were quantitated with the QuantiTect SYBR Green PCR kit (QIAGEN). .. Fluorescence profiles were collected on an ABI 7700 real-time thermal cycler and analyzed with SDS v1.91 (Applied Biosystems).

    Article Title: Matrix mineralization controls gene expression in osteoblastic cells
    Article Snippet: .. For QRT-PCR the QuantiTect SYBR Green PCR Kit (Qiagen) in combination with the StepOnePlus Real-Time PCR System (Applied Biosystems, Darmstadt, Germany) was employed. .. The following ten commercial primer sets QuantiTect Primer Assay (Qiagen) were used to detect targets of interest: HMCN1 (Hs_HMCN1_1_SG, Cat. no.: QT00011228), NID2 (Hs_NID2_1_SG, Cat. no.: QT00055258), ITGA2 (Hs_ITGA2_1_SG, Cat. no.: QT00086695), VCAN (Hs_VCAN_1_SG, Cat. no.: QT00064064), GAPDH (Hs_GAPDH_1_SG, Cat. no.: QT00079247) and ACTB (Hs_ACTB_1_SG, Cat. no.: QT00095431).

    Article Title: Mutation of the Plastidial ?-Glucan Phosphorylase Gene in Rice Affects the Synthesis and Structure of Starch in the Endosperm [W]
    Article Snippet: .. An aliquot of the first-strand cDNA mixture corresponding to 5 ng of total RNA served as the template for quantitative real-time RT-PCR analysis with the Quantitect SYBR Green PCR kit (Qiagen). .. Reactions were performed on an iCycler (Bio-Rad) according to the manufacturer's protocols.

    Article Title: Translational regulation of glutathione peroxidase 4 expression through guanine-rich sequence-binding factor 1 is essential for embryonic brain development
    Article Snippet: .. The chemicals used were from the following sources: SuperScript III reverse transcriptase and RNaseOUT from Invitrogen; BD Advantage 2 Polymerase Mix from BD Biosciences (Pharmingen); dNTPs from Carl Roth GmbH; QuantiTect SYBR Green PCR Kit from Qiagen; and PCR primers from BIOTEZ. .. The yeast strains YBZ-1 and R40coat as well as the plasmid pIIIA/MS2-1 were a generous gift from D. Bernstein (University Wisconsin–Madison, Madison).

    Article Title: Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control 1Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control 1 [W]
    Article Snippet: .. Q-RT-PCR was performed in a 10- μ L reaction using a Quantitect SYBR Green PCR kit (Qiagen) in ABI PRISM 7700 sequence detection system (Applied Biosystems) under the following conditions: 95°C, 10 min; 95°C, 15 s; and 60°C, 1 min. .. Five genes ( ) with a constitutive expression were used for data normalization.

    Article Title: Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster
    Article Snippet: .. Real-time PCR was conducted on a RotorGene-3000 (Corbett Research, Sydney) using a QuantiTect SYBR Green PCR kit (Qiagen, Valencia, CA). .. PCR conditions were 95°C for 10 min to activate the hot-start polymerase, followed by 50 cycles of 95°C for 20 sec, 55°C for 30 sec, and 72°C for 30 sec. Fluorescence was measured after each cycle.

    Article Title: Mutations in the Spike Protein of Middle East Respiratory Syndrome Coronavirus Transmitted in Korea Increase Resistance to Antibody-Mediated Neutralization
    Article Snippet: .. Subsequently, 1 μl of each sample was subjected to quantitative PCR (qPCR) using the QuantiTect SYBR green PCR kit (Qiagen) and a Rotor-Gene Q platform (Qiagen). .. Subsequently, 1 μl of each sample was subjected to quantitative PCR (qPCR) using the QuantiTect SYBR green PCR kit (Qiagen) and a Rotor-Gene Q platform (Qiagen).

    Multiplex Assay:

    Article Title: MarA-Like Regulator of Multidrug Resistance in Yersinia pestis
    Article Snippet: The real-time PCR assay was carried out using the Quantitect SYBR Green PCR kit (QIAGEN). .. The primers either to acrAB Yp (SACABF-GCCGGTGATCGCCTGATTA and SACABR-ATGGTCGGATATTGCGCTAC), marA47 Yp (SA47F-CTATATCCGTGGGCGAGTGT and SA47R-GCTTGATTTCCGGCGTATAA), or YP16s (S16VF-CAGAAGAAGCACCGGCTAAC and S16VR-CGGGGATTTCACATCTGACT) rRNA were used for the real-time PCR carried out in the MX4000 multiplex quantitative PCR system from Stratagene.

    Agarose Gel Electrophoresis:

    Article Title: Firefly luciferase gene contains a cryptic promoter
    Article Snippet: Either 500 ng of mRNA or 15 μg of total RNA were run on a 1% agarose gel according the protocol described in . .. Two microliters of reverse transcriptase reaction were subjected to PCR amplification (15 min at 95°C; then 40 cycles of 30 sec at 94°C; 30 sec at 54°C; 1 min at 68°C; and finally, 4 min at 72°C) using a LightCycler 1.5 (Roche) and QuantiTect SYBR Green PCR kit (QIAGEN).

    CTG Assay:

    Article Title: Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster
    Article Snippet: Real-time PCR was conducted on a RotorGene-3000 (Corbett Research, Sydney) using a QuantiTect SYBR Green PCR kit (Qiagen, Valencia, CA). .. PCR primers used were RpL11F (CGA TCC CTC CAT CGG TAT CT) and RpL11R (AAC CAC TTC ATG GCA TCC TC) for RPL11 , Cyp12a4F (CCA ATC GTC CAG GCA ACT AT) and Cyp12a4R (TCG GGA TCT CTC AGT TCG AG) for Cyp12a4 , and Cyp12a5F (ATC CTG GGG AGA CTT TCG AT) and Cyp12a5R (GCG GTT AAT GGT TTC CAA GA) for Cyp12a5 .

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    Qiagen quantitect sybr green pcr kit
    Quantitect Sybr Green Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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