yeast kloeckera corticis  (ATCC)

Journal: Frontiers in Oncology

Article Title: CASP4 can be a diagnostic biomarker and correlated with immune infiltrates in gliomas

doi: 10.3389/fonc.2022.1025065

Figure Lengend Snippet: Expression patterns of CASP4 in gliomas. (A) Differential expression of CASP4 in different disease states (malignant or benign). The mRNA expression of CASP4 is markedly higher in 689 gliomas in The Cancer Genome Atlas (TCGA) database than in 1,157 normal tissues in the GETx. (B, C) Differential expression of CASP4 in different WHO grades. (B) TCGA database. (C) Chinese Glioma Genome Atlas database. CASP4 expression is significantly different from WHO G2 to G4, and the expression increased as the grade increased. (D–H) CASP4 expression at the mRNA and protein levels is verified using (D,E) western blot, (F) qRT-PCR, and (G,H) immunohistochemical staining (10 × 20). The results show that there is no significant difference between them (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). LGG, low-grade glioma; GBM, glioblastoma.

Article Snippet: Primary antibodies were incubated overnight at 4°C with rabbit anti-human CASP4 monoclonal antibody (PA5-20109, Thermo Fisher).

Techniques: Expressing, Quantitative Proteomics, Western Blot, Quantitative RT-PCR, Immunohistochemical staining, Staining

Journal: Frontiers in Oncology

Article Title: CASP4 can be a diagnostic biomarker and correlated with immune infiltrates in gliomas

doi: 10.3389/fonc.2022.1025065

Figure Lengend Snippet: Clinicopathological characteristics associated with CASP4 expression in glioma patients in The Cancer Genome Atlas (TCGA) database. (A–C) Association between CASP4 expression and survival. (A) Overall survival. (B) Progression-free survival. (C) Disease-free survival. (D) Correlation between CASP4 expression and primary treatment outcome. (E) Histological type of glioma correlated with CASP4 expression (* P < 0.05; ** P < 0.01; *** P < 0.001; NS, P > 0.05). NS is no significance.

Article Snippet: Primary antibodies were incubated overnight at 4°C with rabbit anti-human CASP4 monoclonal antibody (PA5-20109, Thermo Fisher).

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: CASP4 can be a diagnostic biomarker and correlated with immune infiltrates in gliomas

doi: 10.3389/fonc.2022.1025065

Figure Lengend Snippet: Survival curve, receiver operating characteristic (ROC) curve, and overall survival (OS) nomogram in The Cancer Genome Atlas (TCGA) database and Chinese Glioma Genome Atlas (CGGA) database suggest that high CASP4 expression is related to different disease states (tumor or normal) and overall survival. (A) Kaplan–Meier curves of OS in the training set (TCGA). (B) OS in the CGGA dataset. (C–E) OS by WHO grade in glioma patients in TCGA. (C) G2. (D) G3. (E) G4. (F) Time-dependent ROC curves for the 1-, 3-, and 5-year survival in TCGA cohort. (G) Nomogram for predicting the probability of 1-, 3-, and 5-year OS of glioma patients. (H) Calibration plot of the nomogram for predicting the probability of OS.

Article Snippet: Primary antibodies were incubated overnight at 4°C with rabbit anti-human CASP4 monoclonal antibody (PA5-20109, Thermo Fisher).

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: CASP4 can be a diagnostic biomarker and correlated with immune infiltrates in gliomas

doi: 10.3389/fonc.2022.1025065

Figure Lengend Snippet: Forest plot for the hazard ratios of CASP4 expression. Association between CASP4 expression and clinical pathological factors based on univariate Cox regression analysis.

Article Snippet: Primary antibodies were incubated overnight at 4°C with rabbit anti-human CASP4 monoclonal antibody (PA5-20109, Thermo Fisher).

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: CASP4 can be a diagnostic biomarker and correlated with immune infiltrates in gliomas

doi: 10.3389/fonc.2022.1025065

Figure Lengend Snippet: Comparison of immune infiltration in The Cancer Genome Atlas cohort. (A) Correlation between infiltrating immune cell types in gliomas and CASP4 expression. (B–G) Scatter plot of infiltrating immune cells showing positive correlations with CASP4 expression. (H–M) Scatter plot of infiltrating immune cells showing negative correlations with CASP4 expression.

Article Snippet: Primary antibodies were incubated overnight at 4°C with rabbit anti-human CASP4 monoclonal antibody (PA5-20109, Thermo Fisher).

Techniques: Comparison, Expressing

Journal: Frontiers in Oncology

Article Title: CASP4 can be a diagnostic biomarker and correlated with immune infiltrates in gliomas

doi: 10.3389/fonc.2022.1025065

Figure Lengend Snippet: Protein–protein interactions (PPI) and functional analysis of CASP4 and its co-expressed genes in gliomas. (A) The PPI analysis of 61 binding proteins is performed using the STRING software. (B) Volcano plot of differentially expressed genes between the high- and low-CASP4-expression groups. (C) Heat map of the top 10 significantly differentially expressed genes between the high- and low-CASP4-expression groups. (D) Venn diagram of the above-mentioned two groups showing two common members, namely, CASP1 and GSDMD.

Article Snippet: Primary antibodies were incubated overnight at 4°C with rabbit anti-human CASP4 monoclonal antibody (PA5-20109, Thermo Fisher).

Techniques: Protein-Protein interactions, Functional Assay, Binding Assay, Software, Expressing

Journal: Frontiers in Oncology

Article Title: CASP4 can be a diagnostic biomarker and correlated with immune infiltrates in gliomas

doi: 10.3389/fonc.2022.1025065

Figure Lengend Snippet: Functional analysis of CASP4 in patients with gliomas. (A–C) GO analysis of CASP4 co-expression genes. (A) Biological process. (B) Cellular component. (C) Molecular function. (D) KEGG pathway enrichment analysis of CASP4 co-expression genes. GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes.

Article Snippet: Primary antibodies were incubated overnight at 4°C with rabbit anti-human CASP4 monoclonal antibody (PA5-20109, Thermo Fisher).

Techniques: Functional Assay, Expressing

Journal: Frontiers in Oncology

Article Title: CASP4 can be a diagnostic biomarker and correlated with immune infiltrates in gliomas

doi: 10.3389/fonc.2022.1025065

Figure Lengend Snippet: Enrichment pathways from Gene Set Enrichment Analysis. (A) Neutrophil degranulation. (B) Antigen processing and presentation. (C) TOLL-like receptor signaling pathway. (D) NOD-like receptor signaling pathway. (E) Natural-killer-cell-mediated cytotoxicity. (F) Cytokine–cytokine receptor interaction. (G) FCERI-mediated NF-kB activation. (H) IL18 signaling pathway. (I) Cell cycle checkpoints. (J) M phase. All the above-mentioned qualified signaling pathways are significantly enriched in the high-CASP4-expression phenotype.

Article Snippet: Primary antibodies were incubated overnight at 4°C with rabbit anti-human CASP4 monoclonal antibody (PA5-20109, Thermo Fisher).

Techniques: Activation Assay, Protein-Protein interactions, Expressing

Journal: Frontiers in Oncology

Article Title: CASP4 can be a diagnostic biomarker and correlated with immune infiltrates in gliomas

doi: 10.3389/fonc.2022.1025065

Figure Lengend Snippet: The schematic model of CASP4 affects the distribution of microglia and the proliferation of gliomas.

Article Snippet: Primary antibodies were incubated overnight at 4°C with rabbit anti-human CASP4 monoclonal antibody (PA5-20109, Thermo Fisher).

Techniques: