type ii collagen  (Chondrex Inc)

Journal: Cancers

Article Title: Increased EGFR/HER2 Pathway Activation Contributes to Skin Tumorigenesis in Tpl2 − /− Mice

doi: 10.3390/cancers17203362

Figure Lengend Snippet: ErbB Receptor Upregulation in Tpl2 −/− Keratinocytes and Compensatory Response to Inhibitor Treatment. ( A ) Real-time PCR analysis of EGFR, HER2, and HER3 expression in primary keratinocytes from Tpl2 +/+ and Tpl2 −/− keratinocytes. Error bars represent SD. ( B ) Western analyses for p-EGFR, EGFR, HER2 and HER3. Tpl2 +/+ and Tpl2 −/− keratinocytes were cultured in low calcium growth media and upon 90% confluency treated with vehicle-control, 1 μM Lapatinib or 1 μM Gefitinib for 15 min (for phosphorylated protein) or 24 h (for assessing total protein) prior to isolation. NIH Image J was used for densitometry and bands were normalized to beta-actin, which served as a houskeeping gene. Densitometry changes in normalized protein (% of Tpl2 +/+ control) are displayed below each blot. Original western blots are presented in .

Article Snippet: Keratinocytes were grown at 37 °C and 5% CO 2 in keratinocyte growth media (Promocell; Heidelberg, Germany) containing hormone supplements, Penicillin-Streptomycin (10,000 U/mL) and low (0.06 mM) calcium.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Cell Culture, Control, Isolation

Journal: Cancers

Article Title: Increased EGFR/HER2 Pathway Activation Contributes to Skin Tumorigenesis in Tpl2 − /− Mice

doi: 10.3390/cancers17203362

Figure Lengend Snippet: ErbB receptors (EGFR, HER2, HER3) and microRNAs 205 and 21 are upregulated in keratinocytes from Tpl2 −/− mice. ( A ) Schematic of ErbB-related miRs 7, 125, 21, and 205 and their targets. Dashed line denotes that HER2 overexpression can modulate levels of miR21 ( B ) abundance (in nanomolar concentration) of miR21 and respective fold change ( C ) abundance (in nanomolar concentration) of miR205 in Tpl2 +/+ and Tpl2 −/− keratinocytes and respective fold change. Box and whisker plots show the median miR abundance, with box edges representing the 25th and 75th quartiles. Respective fold change displays mean miR abundance.

Article Snippet: Keratinocytes were grown at 37 °C and 5% CO 2 in keratinocyte growth media (Promocell; Heidelberg, Germany) containing hormone supplements, Penicillin-Streptomycin (10,000 U/mL) and low (0.06 mM) calcium.

Techniques: Over Expression, Concentration Assay, Whisker Assay