2 phenylethanol  (Millipore)


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    Name:
    2 Phenylethanol
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    Catalog Number:
    77861
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    Structured Review

    Millipore 2 phenylethanol
    2 Phenylethanol

    https://www.bioz.com/result/2 phenylethanol/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2 phenylethanol - by Bioz Stars, 2020-11
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    Images

    1) Product Images from "Capric Acid Secreted by S. boulardii Inhibits C. albicans Filamentous Growth, Adhesion and Biofilm Formation"

    Article Title: Capric Acid Secreted by S. boulardii Inhibits C. albicans Filamentous Growth, Adhesion and Biofilm Formation

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0012050

    GC-MS analysis of active fraction separated from S. boulardii extract. Peak at 3.98 min corresponds to presence of caproic acid (C6:0), peak at 5.13 min to 2-phenylethanol, peak at 5.32 min to caprylic acid (C8:0), peak at 6.64 min to capric acid (C10:0).
    Figure Legend Snippet: GC-MS analysis of active fraction separated from S. boulardii extract. Peak at 3.98 min corresponds to presence of caproic acid (C6:0), peak at 5.13 min to 2-phenylethanol, peak at 5.32 min to caprylic acid (C8:0), peak at 6.64 min to capric acid (C10:0).

    Techniques Used: Gas Chromatography-Mass Spectrometry

    UPLC chromatograms of 2-phenylethanol and fatty acids as compared with S. boulardii extract monitored at 220 nm. Solid line represents 96 µg of S. boulardii extract, dashed line 5.1 µg of 2-phenylethanol, dotted line 17.4 µg of caproic acid (C6:0), dash-dot-dot line 21.4 µg of caprylic acid (C8:0), dash-dot line 25.8 µg of capric acid (C10:0).
    Figure Legend Snippet: UPLC chromatograms of 2-phenylethanol and fatty acids as compared with S. boulardii extract monitored at 220 nm. Solid line represents 96 µg of S. boulardii extract, dashed line 5.1 µg of 2-phenylethanol, dotted line 17.4 µg of caproic acid (C6:0), dash-dot-dot line 21.4 µg of caprylic acid (C8:0), dash-dot line 25.8 µg of capric acid (C10:0).

    Techniques Used:

    Effect of the compounds on C. albicans adhesion. (A) control sample with 1% methanol, (B) 160 µg/ml of S. boulardii extract, (C) 37.8 µg/ml of caproic acid (C6:0), (D) 17.9 µg/ml of caprylic acid (C8:0), (E) 45.3 µg/ml of capric acid (C10:0), (F) 4.2 µg/ml of 2-phenylethanol, (G) mixture of all these compounds at concentrations indicated above. Statistical analysis was performed using paired Student t -test. P values
    Figure Legend Snippet: Effect of the compounds on C. albicans adhesion. (A) control sample with 1% methanol, (B) 160 µg/ml of S. boulardii extract, (C) 37.8 µg/ml of caproic acid (C6:0), (D) 17.9 µg/ml of caprylic acid (C8:0), (E) 45.3 µg/ml of capric acid (C10:0), (F) 4.2 µg/ml of 2-phenylethanol, (G) mixture of all these compounds at concentrations indicated above. Statistical analysis was performed using paired Student t -test. P values

    Techniques Used:

    C. albicans morphology incubated for 2 h (left panel) and 24 h (right panel) in RPMI-1640 at 37°C in the presence of the compounds. Control sample contained 1% methanol, other samples contained 160 µg/ml of S. boulardii extract, 17.9 µg/ml of caprylic acid (C8:0), 45.3 µg/ml of capric acid (C10:0) or 4.2 µg/ml of 2-phenylethanol. MIX – mixture of all the compounds at concentrations indicated above. Bar, 10 µm.
    Figure Legend Snippet: C. albicans morphology incubated for 2 h (left panel) and 24 h (right panel) in RPMI-1640 at 37°C in the presence of the compounds. Control sample contained 1% methanol, other samples contained 160 µg/ml of S. boulardii extract, 17.9 µg/ml of caprylic acid (C8:0), 45.3 µg/ml of capric acid (C10:0) or 4.2 µg/ml of 2-phenylethanol. MIX – mixture of all the compounds at concentrations indicated above. Bar, 10 µm.

    Techniques Used: Incubation

    2) Product Images from "Novel repellents for the blood-sucking insects Rhodnius prolixus and Triatoma infestans, vectors of Chagas disease"

    Article Title: Novel repellents for the blood-sucking insects Rhodnius prolixus and Triatoma infestans, vectors of Chagas disease

    Journal: bioRxiv

    doi: 10.1101/789602

    Box plots showing the effect of different doses of the test compounds on the time that R. prolixus spent in the proximity of a vertebrate host when the insects were exposed to 2-mercaptoethanol, dimethyl sulfide, 2-phenylethanol, and DEET (median, maximum and minimum values are shown). Letters denote significant differences among treatments according to Dunn’s multiple comparison test (p
    Figure Legend Snippet: Box plots showing the effect of different doses of the test compounds on the time that R. prolixus spent in the proximity of a vertebrate host when the insects were exposed to 2-mercaptoethanol, dimethyl sulfide, 2-phenylethanol, and DEET (median, maximum and minimum values are shown). Letters denote significant differences among treatments according to Dunn’s multiple comparison test (p

    Techniques Used:

    3) Product Images from "The Common Natural Products (S)-α-Terpineol and (E)-2-Hexenol are Important Pheromone Components of Megacyllene antennata (Coleoptera: Cerambycidae)"

    Article Title: The Common Natural Products (S)-α-Terpineol and (E)-2-Hexenol are Important Pheromone Components of Megacyllene antennata (Coleoptera: Cerambycidae)

    Journal: Environmental Entomology

    doi: 10.1093/ee/nvy126

    Representative total ion chromatogram of headspace volatiles from adult males of M. antennata collected in Sonora, Mexico (DB-5 GC column). Compound identifications: 1 ) ( E )-2-hexenal; 2 ) and 3 ) ( E )-2-hexenol and hexanol; 4 ) limonene; 5 ) 1-phenylethanol; 6 ) terpinolene; 7 ) 2-phenylethanol; and 8 ) ( S )-α-terpineol. Peak X is a contaminant.
    Figure Legend Snippet: Representative total ion chromatogram of headspace volatiles from adult males of M. antennata collected in Sonora, Mexico (DB-5 GC column). Compound identifications: 1 ) ( E )-2-hexenal; 2 ) and 3 ) ( E )-2-hexenol and hexanol; 4 ) limonene; 5 ) 1-phenylethanol; 6 ) terpinolene; 7 ) 2-phenylethanol; and 8 ) ( S )-α-terpineol. Peak X is a contaminant.

    Techniques Used:

    4) Product Images from "Capric Acid Secreted by S. boulardii Inhibits C. albicans Filamentous Growth, Adhesion and Biofilm Formation"

    Article Title: Capric Acid Secreted by S. boulardii Inhibits C. albicans Filamentous Growth, Adhesion and Biofilm Formation

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0012050

    GC-MS analysis of active fraction separated from S. boulardii extract. Peak at 3.98 min corresponds to presence of caproic acid (C6:0), peak at 5.13 min to 2-phenylethanol, peak at 5.32 min to caprylic acid (C8:0), peak at 6.64 min to capric acid (C10:0).
    Figure Legend Snippet: GC-MS analysis of active fraction separated from S. boulardii extract. Peak at 3.98 min corresponds to presence of caproic acid (C6:0), peak at 5.13 min to 2-phenylethanol, peak at 5.32 min to caprylic acid (C8:0), peak at 6.64 min to capric acid (C10:0).

    Techniques Used: Gas Chromatography-Mass Spectrometry

    UPLC chromatograms of 2-phenylethanol and fatty acids as compared with S. boulardii extract monitored at 220 nm. Solid line represents 96 µg of S. boulardii extract, dashed line 5.1 µg of 2-phenylethanol, dotted line 17.4 µg of caproic acid (C6:0), dash-dot-dot line 21.4 µg of caprylic acid (C8:0), dash-dot line 25.8 µg of capric acid (C10:0).
    Figure Legend Snippet: UPLC chromatograms of 2-phenylethanol and fatty acids as compared with S. boulardii extract monitored at 220 nm. Solid line represents 96 µg of S. boulardii extract, dashed line 5.1 µg of 2-phenylethanol, dotted line 17.4 µg of caproic acid (C6:0), dash-dot-dot line 21.4 µg of caprylic acid (C8:0), dash-dot line 25.8 µg of capric acid (C10:0).

    Techniques Used:

    Effect of the compounds on C. albicans adhesion. (A) control sample with 1% methanol, (B) 160 µg/ml of S. boulardii extract, (C) 37.8 µg/ml of caproic acid (C6:0), (D) 17.9 µg/ml of caprylic acid (C8:0), (E) 45.3 µg/ml of capric acid (C10:0), (F) 4.2 µg/ml of 2-phenylethanol, (G) mixture of all these compounds at concentrations indicated above. Statistical analysis was performed using paired Student t -test. P values
    Figure Legend Snippet: Effect of the compounds on C. albicans adhesion. (A) control sample with 1% methanol, (B) 160 µg/ml of S. boulardii extract, (C) 37.8 µg/ml of caproic acid (C6:0), (D) 17.9 µg/ml of caprylic acid (C8:0), (E) 45.3 µg/ml of capric acid (C10:0), (F) 4.2 µg/ml of 2-phenylethanol, (G) mixture of all these compounds at concentrations indicated above. Statistical analysis was performed using paired Student t -test. P values

    Techniques Used:

    C. albicans morphology incubated for 2 h (left panel) and 24 h (right panel) in RPMI-1640 at 37°C in the presence of the compounds. Control sample contained 1% methanol, other samples contained 160 µg/ml of S. boulardii extract, 17.9 µg/ml of caprylic acid (C8:0), 45.3 µg/ml of capric acid (C10:0) or 4.2 µg/ml of 2-phenylethanol. MIX – mixture of all the compounds at concentrations indicated above. Bar, 10 µm.
    Figure Legend Snippet: C. albicans morphology incubated for 2 h (left panel) and 24 h (right panel) in RPMI-1640 at 37°C in the presence of the compounds. Control sample contained 1% methanol, other samples contained 160 µg/ml of S. boulardii extract, 17.9 µg/ml of caprylic acid (C8:0), 45.3 µg/ml of capric acid (C10:0) or 4.2 µg/ml of 2-phenylethanol. MIX – mixture of all the compounds at concentrations indicated above. Bar, 10 µm.

    Techniques Used: Incubation

    5) Product Images from "Volatile Compounds from Grape Skin, Juice and Wine from Five Interspecific HybridGrape Cultivars Grown in Québec (Canada) for Wine Production"

    Article Title: Volatile Compounds from Grape Skin, Juice and Wine from Five Interspecific HybridGrape Cultivars Grown in Québec (Canada) for Wine Production

    Journal: Molecules

    doi: 10.3390/molecules200610980

    Biplots of four redundancy analyses relating berry volatile compounds (juice and berry skin, independent variables, yellow circles) to wine volatile compounds (dependant variables, red triangles) in the interspecific hybrid grape varieties Frontenac, Marquette, Maréchal Foch, Sabrevois and St. Croix ( n = 32), for the following groups of compounds: Fatty acid degradation products ( A ), Terpenes and C 13 -norisoprenoids ( B ); Non-aromatic acids, esters, alcohol and acetates ( C ); and Aromatics ( D ). The variables are identified as follow: ( A ) Berry juice (_J) and skin variables (_Sk): hexanal (C6CHO_J; C6CHO_Sk), ( Z )-3-hexenal (Z3C6_J), ( E )-2-hexenal (E2C6_J; E2C6_Sk), 2-octanone (C8one_J), ( E )-2-heptenal (E2C7_J), hexanol (C6OH_J; C6OH_Sk), ( Z )-3-hexenol (Z3C6OH_J; Z3C6OH_Sk), ( E , E )-2,4-hexadienal (EEC6_J), ( E )-2-hexenol (juice: 2; E2C6OH_Sk), 1-octen-3-ol (C8OH_J; C8OH_Sk), ( E , E )-2,4-heptadienal (juice: 1), ( E , Z )-2,4-heptadienal (juice: 3), decanal (dec_J), ( E,Z )-2,6-nonadienal (non_J), 2-undecanone (und_J); Wine variables: hexanal (C6CHO_W), hexanol (C6OH_W), ( E )-3-hexenol (E3C6OH_W), ( Z )-3-hexenol (Z3C6OH_W), nonanal (C9CHO_W), ( E , Z )-2,6-nonadienal (wine: 4); ( B ) Berry juice (_J) and skin variables (_Sk): β-myrcene (bmyrc_J; bmyrc_Sk), ( R )-(+)-limonene (limon_J; limon_Sk), linalool (linal_J; linal_Sk), α-terpineol (aterp_J), β-citronellol (bcitron_Sk), nerol (nerol_Sk), β-damascenone (bdam_J; bdam_Sk), α-ionone (aiono_J), α-ionol (aionol_J), β-ionone (biono_J; biono_Sk); Wine variables: β-myrcene (bmyrc_W), p -cymenene (pcymen_W), linalool (linal_W), α-terpineol (aterp_W), β-citronellol (bcitron_W), nerol (nerol_W), geraniol (ger_W), β-damascenone (bdam_W); ( C ) Berry juice (_J) and skin variables (_Sk): ethyl propanoate (etpro_J), ethyl 2-methylpropanoate (etibut_J), ethyl butanoate (etbut_J; etbut_Sk), ethyl 2-methylbutanoate (et2MB_J; et2MB_Sk), ethyl 3-methylbutanoate (etival_J), ethyl ( E )-2-butenoate (etcro_J; etcro_Sk), ethyl hexanoate (ethex_J), isoamyl acetate (iaAce_J); Wine variables: ethyl 2-methylpropanoate (etibut_W), ethyl butanoate (etbut_W), ethyl-2-methylbutanoate (et2MB_W), ethyl 3-methylbutanoate (etiva_W), ethyl hexanoate (ethex_W), ethyl octanoate (etoct_W), ethyl decanoate (etdec_W), ethyl-3-hydroxyhexanoate (et3OHH_W), hexanoic acid (hexac_W), octanoic acid (octac_W), isobutyl acetate (ibAce_W), isoamyl acetate (iaAce_W), hexyl acetate (hAce_W), ethyl lactate (etlact_W), acetoin (ace_W), butyrolactone (butlac_W), isobutanol (ibutOH_W); ( D ) Berry juice (_J) and skin variables (_Sk): 2-phenylacetaldehyde (PhCHO_J, PhCHO_Sk), 2-phenylethanol (PhEtOH_J; PhEtOH_Sk), eugenol (eug_J, eug_Sk), p -vinylguaiacol (VG_J); Wine variables: eugenol (eug_W), p -vinylguaiacol (VG_W), ethyl phenylacetate (etPhAce_W), phenethyl acetate (PhAc_W), ethyl dihydrocinnamate (etdhcin_W), ethyl cinnamate (etcin_W), ethyl vanillate (etvan_W), 2-phenylethanol (PhEtOH_W).
    Figure Legend Snippet: Biplots of four redundancy analyses relating berry volatile compounds (juice and berry skin, independent variables, yellow circles) to wine volatile compounds (dependant variables, red triangles) in the interspecific hybrid grape varieties Frontenac, Marquette, Maréchal Foch, Sabrevois and St. Croix ( n = 32), for the following groups of compounds: Fatty acid degradation products ( A ), Terpenes and C 13 -norisoprenoids ( B ); Non-aromatic acids, esters, alcohol and acetates ( C ); and Aromatics ( D ). The variables are identified as follow: ( A ) Berry juice (_J) and skin variables (_Sk): hexanal (C6CHO_J; C6CHO_Sk), ( Z )-3-hexenal (Z3C6_J), ( E )-2-hexenal (E2C6_J; E2C6_Sk), 2-octanone (C8one_J), ( E )-2-heptenal (E2C7_J), hexanol (C6OH_J; C6OH_Sk), ( Z )-3-hexenol (Z3C6OH_J; Z3C6OH_Sk), ( E , E )-2,4-hexadienal (EEC6_J), ( E )-2-hexenol (juice: 2; E2C6OH_Sk), 1-octen-3-ol (C8OH_J; C8OH_Sk), ( E , E )-2,4-heptadienal (juice: 1), ( E , Z )-2,4-heptadienal (juice: 3), decanal (dec_J), ( E,Z )-2,6-nonadienal (non_J), 2-undecanone (und_J); Wine variables: hexanal (C6CHO_W), hexanol (C6OH_W), ( E )-3-hexenol (E3C6OH_W), ( Z )-3-hexenol (Z3C6OH_W), nonanal (C9CHO_W), ( E , Z )-2,6-nonadienal (wine: 4); ( B ) Berry juice (_J) and skin variables (_Sk): β-myrcene (bmyrc_J; bmyrc_Sk), ( R )-(+)-limonene (limon_J; limon_Sk), linalool (linal_J; linal_Sk), α-terpineol (aterp_J), β-citronellol (bcitron_Sk), nerol (nerol_Sk), β-damascenone (bdam_J; bdam_Sk), α-ionone (aiono_J), α-ionol (aionol_J), β-ionone (biono_J; biono_Sk); Wine variables: β-myrcene (bmyrc_W), p -cymenene (pcymen_W), linalool (linal_W), α-terpineol (aterp_W), β-citronellol (bcitron_W), nerol (nerol_W), geraniol (ger_W), β-damascenone (bdam_W); ( C ) Berry juice (_J) and skin variables (_Sk): ethyl propanoate (etpro_J), ethyl 2-methylpropanoate (etibut_J), ethyl butanoate (etbut_J; etbut_Sk), ethyl 2-methylbutanoate (et2MB_J; et2MB_Sk), ethyl 3-methylbutanoate (etival_J), ethyl ( E )-2-butenoate (etcro_J; etcro_Sk), ethyl hexanoate (ethex_J), isoamyl acetate (iaAce_J); Wine variables: ethyl 2-methylpropanoate (etibut_W), ethyl butanoate (etbut_W), ethyl-2-methylbutanoate (et2MB_W), ethyl 3-methylbutanoate (etiva_W), ethyl hexanoate (ethex_W), ethyl octanoate (etoct_W), ethyl decanoate (etdec_W), ethyl-3-hydroxyhexanoate (et3OHH_W), hexanoic acid (hexac_W), octanoic acid (octac_W), isobutyl acetate (ibAce_W), isoamyl acetate (iaAce_W), hexyl acetate (hAce_W), ethyl lactate (etlact_W), acetoin (ace_W), butyrolactone (butlac_W), isobutanol (ibutOH_W); ( D ) Berry juice (_J) and skin variables (_Sk): 2-phenylacetaldehyde (PhCHO_J, PhCHO_Sk), 2-phenylethanol (PhEtOH_J; PhEtOH_Sk), eugenol (eug_J, eug_Sk), p -vinylguaiacol (VG_J); Wine variables: eugenol (eug_W), p -vinylguaiacol (VG_W), ethyl phenylacetate (etPhAce_W), phenethyl acetate (PhAc_W), ethyl dihydrocinnamate (etdhcin_W), ethyl cinnamate (etcin_W), ethyl vanillate (etvan_W), 2-phenylethanol (PhEtOH_W).

    Techniques Used:

    Redundancy analysis relating the chemical composition of the berry skin and juice (independent variables) to the chemical composition of the wines (dependant variables) made from the interspecific hybrid grape varieties Frontenac (blue diamonds), Marquette (red squares), Maréchal Foch (green triangles), Sabrevois (purple stars) and St. Croix (yellow circles). ( A ) Varieties samples plot ( n = 32); ( B ) Biplot of grape (yellow circles, 14 variables) and wine (red triangles, 10 variables). The berry skin and juice variables are identified as follow: total soluble solids (Brix_J), titratable acidity (TA_J), pH (pH_J), sum of fatty acids degradation products in juice (FADP_J) and in skin (FADP_Sk), sum of terpenes in juice (T_J) and in skin (T_Sk), sum of C 13 -norisoprenoids in juice (C13_J) and in skin (C13_Sk), fatty acid ethyl esters in juice (FAEE_J) and in skin (FAEE_Sk), volatile phenols in juice (Ar_J, including 2-phenylacetaldehyde and 2-phenylethanol; VP_J, including eugenol and p -vinylguaiacol) and in skin (VP_Sk, including 2-phenylacetaldehyde, 2-phenylethanol, and eugenol). The wine variables are identified as follow: alcohol percentage (Alc_W), titratable acidity (TA_W), pH (pH_W), sum of fatty acids degradation products (FADP_W), sum of terpenes (T_W), volatile phenols (VP_W, including eugenol and p -vinylguaiacol), fatty acid ethyl esters (FAEE_W), free fatty acids (FFA_W, including hexanoic acid and octanoic acid), phenolic esters (PE_W), C 13 -norisoprenoids (C13_W, including only β-damascenone). For variables grouped as a sum of compounds that are not listed here (e.g., FADP, terpenes, C 13 -norisoprenoids, FAEE, Phenolic esters), see Table 1 , Table 2 and Table 3 for the complete list of compounds grouped in each class.
    Figure Legend Snippet: Redundancy analysis relating the chemical composition of the berry skin and juice (independent variables) to the chemical composition of the wines (dependant variables) made from the interspecific hybrid grape varieties Frontenac (blue diamonds), Marquette (red squares), Maréchal Foch (green triangles), Sabrevois (purple stars) and St. Croix (yellow circles). ( A ) Varieties samples plot ( n = 32); ( B ) Biplot of grape (yellow circles, 14 variables) and wine (red triangles, 10 variables). The berry skin and juice variables are identified as follow: total soluble solids (Brix_J), titratable acidity (TA_J), pH (pH_J), sum of fatty acids degradation products in juice (FADP_J) and in skin (FADP_Sk), sum of terpenes in juice (T_J) and in skin (T_Sk), sum of C 13 -norisoprenoids in juice (C13_J) and in skin (C13_Sk), fatty acid ethyl esters in juice (FAEE_J) and in skin (FAEE_Sk), volatile phenols in juice (Ar_J, including 2-phenylacetaldehyde and 2-phenylethanol; VP_J, including eugenol and p -vinylguaiacol) and in skin (VP_Sk, including 2-phenylacetaldehyde, 2-phenylethanol, and eugenol). The wine variables are identified as follow: alcohol percentage (Alc_W), titratable acidity (TA_W), pH (pH_W), sum of fatty acids degradation products (FADP_W), sum of terpenes (T_W), volatile phenols (VP_W, including eugenol and p -vinylguaiacol), fatty acid ethyl esters (FAEE_W), free fatty acids (FFA_W, including hexanoic acid and octanoic acid), phenolic esters (PE_W), C 13 -norisoprenoids (C13_W, including only β-damascenone). For variables grouped as a sum of compounds that are not listed here (e.g., FADP, terpenes, C 13 -norisoprenoids, FAEE, Phenolic esters), see Table 1 , Table 2 and Table 3 for the complete list of compounds grouped in each class.

    Techniques Used:

    6) Product Images from "Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products"

    Article Title: Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products

    Journal: Microbial Cell Factories

    doi: 10.1186/s12934-020-01461-7

    Shikimate and phenylalanine/tyrosine biosynthesis in Kluyveromyces marxianus . The enzymes are named according to their orthologues in S. cerevisiae . In our study, genes for enzymes coloured red have been deleted or otherwise inactivated and those coloured green are overexpressed, with feedback-resistant alleles represented by the superscript fbr. Red dashed lines indicate product inhibition on enzymes, while dashed arrows indicated multiple reactions between the metabolites indicated. Abbreviations: PEP: phosphoenolpyruvate, E4P: erythrose 4-phosphate, DAHP: 3-deoxy-D-arabinoheptulosonate-7-phosphate, DHQ: 3-dehydroquinate, 3-DHS: 3-dehydroshikimate, SHIK: shikimic acid, S3P: shikimate-3-phosphate EPSP: 5-enoylpyruvateshikimate-3-phosphate, CHOR: chorismate, PPH; prephentate, (H)PPY: (hydroxyl)phenylpyruvate, 2-PE: 2-phenylethanol; PAA: phenylacetic acid; TYROSOL: tyrosol/para-hydroxyphenylethanol; PHPA: para-hydroxphenylacetate; TRP: tryptophan; TYR: tyrosine, PHE: phenylalanine
    Figure Legend Snippet: Shikimate and phenylalanine/tyrosine biosynthesis in Kluyveromyces marxianus . The enzymes are named according to their orthologues in S. cerevisiae . In our study, genes for enzymes coloured red have been deleted or otherwise inactivated and those coloured green are overexpressed, with feedback-resistant alleles represented by the superscript fbr. Red dashed lines indicate product inhibition on enzymes, while dashed arrows indicated multiple reactions between the metabolites indicated. Abbreviations: PEP: phosphoenolpyruvate, E4P: erythrose 4-phosphate, DAHP: 3-deoxy-D-arabinoheptulosonate-7-phosphate, DHQ: 3-dehydroquinate, 3-DHS: 3-dehydroshikimate, SHIK: shikimic acid, S3P: shikimate-3-phosphate EPSP: 5-enoylpyruvateshikimate-3-phosphate, CHOR: chorismate, PPH; prephentate, (H)PPY: (hydroxyl)phenylpyruvate, 2-PE: 2-phenylethanol; PAA: phenylacetic acid; TYROSOL: tyrosol/para-hydroxyphenylethanol; PHPA: para-hydroxphenylacetate; TRP: tryptophan; TYR: tyrosine, PHE: phenylalanine

    Techniques Used: Inhibition

    7) Product Images from "Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products"

    Article Title: Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products

    Journal: bioRxiv

    doi: 10.1101/2020.08.12.247957

    Shikimate and phenylalanine/tyrosine biosynthesis in Kluyeromyces marxianus . The genes are named according to their orthologues in Saccharomyces cerevisiae . Genes in red are deleted or otherwise inactivated, genes in green are overexpressed in our study, and genes in blue are overexpressed alleles of genes resistant to feedback inhibition. Red dashed lines indicate product inhibition, while dashed arrows indicated multiple reactions between the metabolites indicated. Compounds that were assayed by HPLC are italicised. Abbreviations: PEP: phosphoenolpyruvate, E4P: erythrose 4-phosphate, DAHP: 3-deoxy-D-arabinoheptulosonate-7-phosphate, DHQ: 3-dehydroquinate, 3-DHS: 3-dehydroshikimate, SHIK: shikimic acid, S3P: shikimate-3-phosphate EPSP: 5-enoylpyruvateshikimate-3-phosphate, CHOR: chorismate, PPH; prephentate, (H)PPY: (hydroxyl)phenylpyruvate, 2-PE: 2-phenylethanol; PAA: phenylacetic acid; TYROSOL: tyrosol/para-hydroxyphenylethanol; PHPA: para-hydroxphenylacetate; TRP: tryptophan; TYR: tyrosine, PHE: phenylalanine.
    Figure Legend Snippet: Shikimate and phenylalanine/tyrosine biosynthesis in Kluyeromyces marxianus . The genes are named according to their orthologues in Saccharomyces cerevisiae . Genes in red are deleted or otherwise inactivated, genes in green are overexpressed in our study, and genes in blue are overexpressed alleles of genes resistant to feedback inhibition. Red dashed lines indicate product inhibition, while dashed arrows indicated multiple reactions between the metabolites indicated. Compounds that were assayed by HPLC are italicised. Abbreviations: PEP: phosphoenolpyruvate, E4P: erythrose 4-phosphate, DAHP: 3-deoxy-D-arabinoheptulosonate-7-phosphate, DHQ: 3-dehydroquinate, 3-DHS: 3-dehydroshikimate, SHIK: shikimic acid, S3P: shikimate-3-phosphate EPSP: 5-enoylpyruvateshikimate-3-phosphate, CHOR: chorismate, PPH; prephentate, (H)PPY: (hydroxyl)phenylpyruvate, 2-PE: 2-phenylethanol; PAA: phenylacetic acid; TYROSOL: tyrosol/para-hydroxyphenylethanol; PHPA: para-hydroxphenylacetate; TRP: tryptophan; TYR: tyrosine, PHE: phenylalanine.

    Techniques Used: Inhibition, High Performance Liquid Chromatography

    8) Product Images from "The occurrence and formation of monoterpenes in herbivore-damaged poplar roots"

    Article Title: The occurrence and formation of monoterpenes in herbivore-damaged poplar roots

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-36302-6

    The effect of volatile monoterpenes on the growth of Phytophthora cactorum (Oomycetes). Mycelial growth of P. cactorum was measured in the presence of vapor-phase 2-phenylethanol, 1,8-cineole, (−)-limonene, and (−)-β-pinene. Pathogen growth in response to the individual compounds was compared to pathogen growth when exposed to pure mineral oil. The area of P. cactorum mycelium for the control treatment was set at 100% and growth under the influence of the different volatile compounds is shown relative to the mineral oil control. Means ± SE are shown (n = 5). Asterisks indicate significant differences (Student’s t-tests. 2-phenylethanol ( P = 0.452, t = −0.797); 1,8-cineole ( P = 0.001, t = −6.6); (−)-limonene ( P = 0.166, t = 1.524); (−)-β-pinene ( P = 0.023, t = −2.792)).
    Figure Legend Snippet: The effect of volatile monoterpenes on the growth of Phytophthora cactorum (Oomycetes). Mycelial growth of P. cactorum was measured in the presence of vapor-phase 2-phenylethanol, 1,8-cineole, (−)-limonene, and (−)-β-pinene. Pathogen growth in response to the individual compounds was compared to pathogen growth when exposed to pure mineral oil. The area of P. cactorum mycelium for the control treatment was set at 100% and growth under the influence of the different volatile compounds is shown relative to the mineral oil control. Means ± SE are shown (n = 5). Asterisks indicate significant differences (Student’s t-tests. 2-phenylethanol ( P = 0.452, t = −0.797); 1,8-cineole ( P = 0.001, t = −6.6); (−)-limonene ( P = 0.166, t = 1.524); (−)-β-pinene ( P = 0.023, t = −2.792)).

    Techniques Used:

    9) Product Images from "Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products"

    Article Title: Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products

    Journal: Microbial Cell Factories

    doi: 10.1186/s12934-020-01461-7

    Shikimate and phenylalanine/tyrosine biosynthesis in Kluyveromyces marxianus . The enzymes are named according to their orthologues in S. cerevisiae . In our study, genes for enzymes coloured red have been deleted or otherwise inactivated and those coloured green are overexpressed, with feedback-resistant alleles represented by the superscript fbr. Red dashed lines indicate product inhibition on enzymes, while dashed arrows indicated multiple reactions between the metabolites indicated. Abbreviations: PEP: phosphoenolpyruvate, E4P: erythrose 4-phosphate, DAHP: 3-deoxy-D-arabinoheptulosonate-7-phosphate, DHQ: 3-dehydroquinate, 3-DHS: 3-dehydroshikimate, SHIK: shikimic acid, S3P: shikimate-3-phosphate EPSP: 5-enoylpyruvateshikimate-3-phosphate, CHOR: chorismate, PPH; prephentate, (H)PPY: (hydroxyl)phenylpyruvate, 2-PE: 2-phenylethanol; PAA: phenylacetic acid; TYROSOL: tyrosol/para-hydroxyphenylethanol; PHPA: para-hydroxphenylacetate; TRP: tryptophan; TYR: tyrosine, PHE: phenylalanine
    Figure Legend Snippet: Shikimate and phenylalanine/tyrosine biosynthesis in Kluyveromyces marxianus . The enzymes are named according to their orthologues in S. cerevisiae . In our study, genes for enzymes coloured red have been deleted or otherwise inactivated and those coloured green are overexpressed, with feedback-resistant alleles represented by the superscript fbr. Red dashed lines indicate product inhibition on enzymes, while dashed arrows indicated multiple reactions between the metabolites indicated. Abbreviations: PEP: phosphoenolpyruvate, E4P: erythrose 4-phosphate, DAHP: 3-deoxy-D-arabinoheptulosonate-7-phosphate, DHQ: 3-dehydroquinate, 3-DHS: 3-dehydroshikimate, SHIK: shikimic acid, S3P: shikimate-3-phosphate EPSP: 5-enoylpyruvateshikimate-3-phosphate, CHOR: chorismate, PPH; prephentate, (H)PPY: (hydroxyl)phenylpyruvate, 2-PE: 2-phenylethanol; PAA: phenylacetic acid; TYROSOL: tyrosol/para-hydroxyphenylethanol; PHPA: para-hydroxphenylacetate; TRP: tryptophan; TYR: tyrosine, PHE: phenylalanine

    Techniques Used: Inhibition

    10) Product Images from "The diagonal band of broca continually regulates olfactory-mediated behaviors by modulating odor-evoked responses within the olfactory bulb"

    Article Title: The diagonal band of broca continually regulates olfactory-mediated behaviors by modulating odor-evoked responses within the olfactory bulb

    Journal: bioRxiv

    doi: 10.1101/2020.11.07.372649

    Tetanus toxin-mediated silencing of HDB disrupts odor habituation and discrimination without affecting general cognitive abilities. (A) Schematic of the stereotaxic injection. (B) The odor habituation/discrimination test (OHOD) performed in an open-field arena (60 x 60 cm). The circle in the middle of the arena indicates a petri dish containing a paper with odorant covered with fresh bedding. 2-phenylethanol (rose odor) and vanillin were used in habituation and discrimination trials, accordingly. (C) Investigation time (time spent at petri dish, 120 or 240 sec trial duration and 2 min. ITI) of control and HDB silenced mice during odor habituation and discrimination trials (see also Fig. 1 ). (D) Schematic of the stereotaxic injection. (E) Latency (time to reach the petri dish) and investigation time of control and HDB silenced mice during odor habituation and discrimination trials with shorter trial durations. (F) Schematic stereotaxic injection and sequence of experiments. (G) Latency and Investigation time of control and HDB silenced mice during odor habituation and discrimination trials before (blue) and after TeTn virus injection. (H) Schematic of test sequence and stereotaxic injection. (I) Buried food test was conducted in home cage. The ellipse indicates a hidden food pellet. (J) Latency in finding the food pellet of control and HDB silenced mice during buried food test. (K) The puzzle box test paradigm. Left: the arena is divided by black-painted Plexiglas into the start and goal compartments. Right: a timeline of the experiment; the compartments are connected by a door opening (trial 1), open underpass (trials 2-4), underpass blocked by sawdust (trials 5-7), or underpass blocked by a cardboard plug (trials 8-10). (L) Latency (time to enter the goal compartment) of control and HDB silenced mice. 1-way ANOVA followed by Dunnett’s posttest (*) and t-test (#) of sham-injected (n=6) and TeTn-injected (n=6) mice. #, *p
    Figure Legend Snippet: Tetanus toxin-mediated silencing of HDB disrupts odor habituation and discrimination without affecting general cognitive abilities. (A) Schematic of the stereotaxic injection. (B) The odor habituation/discrimination test (OHOD) performed in an open-field arena (60 x 60 cm). The circle in the middle of the arena indicates a petri dish containing a paper with odorant covered with fresh bedding. 2-phenylethanol (rose odor) and vanillin were used in habituation and discrimination trials, accordingly. (C) Investigation time (time spent at petri dish, 120 or 240 sec trial duration and 2 min. ITI) of control and HDB silenced mice during odor habituation and discrimination trials (see also Fig. 1 ). (D) Schematic of the stereotaxic injection. (E) Latency (time to reach the petri dish) and investigation time of control and HDB silenced mice during odor habituation and discrimination trials with shorter trial durations. (F) Schematic stereotaxic injection and sequence of experiments. (G) Latency and Investigation time of control and HDB silenced mice during odor habituation and discrimination trials before (blue) and after TeTn virus injection. (H) Schematic of test sequence and stereotaxic injection. (I) Buried food test was conducted in home cage. The ellipse indicates a hidden food pellet. (J) Latency in finding the food pellet of control and HDB silenced mice during buried food test. (K) The puzzle box test paradigm. Left: the arena is divided by black-painted Plexiglas into the start and goal compartments. Right: a timeline of the experiment; the compartments are connected by a door opening (trial 1), open underpass (trials 2-4), underpass blocked by sawdust (trials 5-7), or underpass blocked by a cardboard plug (trials 8-10). (L) Latency (time to enter the goal compartment) of control and HDB silenced mice. 1-way ANOVA followed by Dunnett’s posttest (*) and t-test (#) of sham-injected (n=6) and TeTn-injected (n=6) mice. #, *p

    Techniques Used: Injection, Mouse Assay, Sequencing

    11) Product Images from "Development of 2-phenylethanol plus acetic acid lures to monitor obliquebanded leafroller (Lepidoptera: Tortricidae) under mating disruption"

    Article Title: Development of 2-phenylethanol plus acetic acid lures to monitor obliquebanded leafroller (Lepidoptera: Tortricidae) under mating disruption

    Journal: bioRxiv

    doi: 10.1101/093955

    Experiment 8: comparison of mean (SE) total and female moth catches of C. rosaceana in delta traps baited with plastic vials with a 3.2-mm aperture in lid loaded with 5.0 ml of acetic acid and proprietary plastic membrane cup lures loaded with either 2-phenylethanol (TRE1256) or phenylacetonitrile (TRE1381).
    Figure Legend Snippet: Experiment 8: comparison of mean (SE) total and female moth catches of C. rosaceana in delta traps baited with plastic vials with a 3.2-mm aperture in lid loaded with 5.0 ml of acetic acid and proprietary plastic membrane cup lures loaded with either 2-phenylethanol (TRE1256) or phenylacetonitrile (TRE1381).

    Techniques Used:

    Experiment 9: comparison of mean (SE) male catches of C. rosaceana in Multipher 1 plastic bucket traps baited with a red rubber septum loaded with the 4-component sex pheromone (TRE1229), or the same septum plus a proprietary plastic membrane cup lure loaded with 2-phenylethanol (TRE1256) or phenylacetonitrile (TRE1381). Propylene glycol (250 ml) was used in the bottom of these traps to retain moths.
    Figure Legend Snippet: Experiment 9: comparison of mean (SE) male catches of C. rosaceana in Multipher 1 plastic bucket traps baited with a red rubber septum loaded with the 4-component sex pheromone (TRE1229), or the same septum plus a proprietary plastic membrane cup lure loaded with 2-phenylethanol (TRE1256) or phenylacetonitrile (TRE1381). Propylene glycol (250 ml) was used in the bottom of these traps to retain moths.

    Techniques Used:

    12) Product Images from "Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products"

    Article Title: Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products

    Journal: bioRxiv

    doi: 10.1101/2020.08.12.247957

    Shikimate and phenylalanine/tyrosine biosynthesis in Kluyeromyces marxianus . The genes are named according to their orthologues in Saccharomyces cerevisiae . Genes in red are deleted or otherwise inactivated, genes in green are overexpressed in our study, and genes in blue are overexpressed alleles of genes resistant to feedback inhibition. Red dashed lines indicate product inhibition, while dashed arrows indicated multiple reactions between the metabolites indicated. Compounds that were assayed by HPLC are italicised. Abbreviations: PEP: phosphoenolpyruvate, E4P: erythrose 4-phosphate, DAHP: 3-deoxy-D-arabinoheptulosonate-7-phosphate, DHQ: 3-dehydroquinate, 3-DHS: 3-dehydroshikimate, SHIK: shikimic acid, S3P: shikimate-3-phosphate EPSP: 5-enoylpyruvateshikimate-3-phosphate, CHOR: chorismate, PPH; prephentate, (H)PPY: (hydroxyl)phenylpyruvate, 2-PE: 2-phenylethanol; PAA: phenylacetic acid; TYROSOL: tyrosol/para-hydroxyphenylethanol; PHPA: para-hydroxphenylacetate; TRP: tryptophan; TYR: tyrosine, PHE: phenylalanine.
    Figure Legend Snippet: Shikimate and phenylalanine/tyrosine biosynthesis in Kluyeromyces marxianus . The genes are named according to their orthologues in Saccharomyces cerevisiae . Genes in red are deleted or otherwise inactivated, genes in green are overexpressed in our study, and genes in blue are overexpressed alleles of genes resistant to feedback inhibition. Red dashed lines indicate product inhibition, while dashed arrows indicated multiple reactions between the metabolites indicated. Compounds that were assayed by HPLC are italicised. Abbreviations: PEP: phosphoenolpyruvate, E4P: erythrose 4-phosphate, DAHP: 3-deoxy-D-arabinoheptulosonate-7-phosphate, DHQ: 3-dehydroquinate, 3-DHS: 3-dehydroshikimate, SHIK: shikimic acid, S3P: shikimate-3-phosphate EPSP: 5-enoylpyruvateshikimate-3-phosphate, CHOR: chorismate, PPH; prephentate, (H)PPY: (hydroxyl)phenylpyruvate, 2-PE: 2-phenylethanol; PAA: phenylacetic acid; TYROSOL: tyrosol/para-hydroxyphenylethanol; PHPA: para-hydroxphenylacetate; TRP: tryptophan; TYR: tyrosine, PHE: phenylalanine.

    Techniques Used: Inhibition, High Performance Liquid Chromatography

    Related Articles

    MTT Assay:

    Article Title: Secondary metabolites from food-derived yeasts inhibit virulence of Candida albicans
    Article Snippet: .. Ltd. RPMI-1640, yeast synthetic drop-out medium supplements, yeast nitrogen base, tryptophol, phenylethanol and 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) were obtained from Sigma-Aldrich. ..

    other:

    Article Title: Novel repellents for the blood-sucking insects Rhodnius prolixus and Triatoma infestans, vectors of Chagas disease
    Article Snippet: Dimethyl sulfide and 2-mercaptoethanol solutions were made in distilled water, while ethanol was the solvent for 2-phenylethanol and DEET.

    Article Title: The Common Natural Products (S)-α-Terpineol and (E)-2-Hexenol are Important Pheromone Components of Megacyllene antennata (Coleoptera: Cerambycidae)
    Article Snippet: All compounds were purchased from commercial suppliers: ( R )- and ( S )-α-terpineol (both > 98% pure), ( E )-2-hexenol (96%), ( E )-2-hexenal (98%), ( R )- and ( S )-limonene (both 96%), and 2-phenylethanol (98%) from Sigma-Aldrich (St. Louis, MO); ( R )- and ( S )-1-phenylethanol (both > 97%) and 1-hexanol (99%) from Alfa Aesar (Ward Hill, MA); and terpinolene (85%) from TCI America (Portland, OR).

    Article Title: Volatile Compounds from Grape Skin, Juice and Wine from Five Interspecific HybridGrape Cultivars Grown in Québec (Canada) for Wine Production
    Article Snippet: Acetoin, butyrolactone, β-citronellol, p -cymenene, β-damascenone, decanal, ethyl butanoate, ethyl cinnamate, ethyl decanoate, ethyl dihydrocinnamate, ethyl-3-hydroxyhexanoate, ethyl 2-methylbutanoate, ethyl 3-methylbutanoate, ethyl 2-methylpropanoate, (−)-ethyl L-lactate, ethyl octanoate, ethyl phenylacetate, ethyl (E )-2-butenoate, eugenol, fructose, geraniol, d -(+)-gluconic acid δ-lactone, glucose, (E ,E )-2,4-heptadienal, (E ,Z )-2,4-heptadienal, 1-heptanol, (E )-2-heptenal, (E ,E )-2,4-hexadienal, hexanal, hexanoic acid, hexanol, (Z )-3-hexenal, (E )-2- hexenal, (Z )-3-hexenol, (E )-2-hexenol, (E )-3-hexenol, hexyl acetate, α-ionol, α-ionone, β-ionone, isobutanol, isoamyl acetate, isobutyl acetate, linalool, (R )-(+)-limonene, nerol, (E ,Z )-2,6-nonadienal, γ-nonalactone, γ-octalactone, octanoic acid, 2-octanone, 1-octen-3-ol, 1-octen-3-one, phenethyl acetate, 2-phenylacetaldehyde, 2-phenylethanol, α-terpineol, 2-undecanone, and p -vinylguaiacol were bought from Sigma-Aldrich (St. Louis, MO, USA).

    Purification:

    Article Title: Enzyme-Modified Particles for Selective Biocatalytic Hydrogenation by Hydrogen-Driven NADH Recycling
    Article Snippet: .. Chemicals Acetophenone (Sigma), phenylethanol (Sigma), NAD+ (Prozomix), acetonitrile (Sigma), carbon black particles (Black Pearls 2000, BP2000, Cabot Corporation) and buffer salts (Sigma) were used as received without further purification. .. All solutions were prepared with MilliQ water (Millipore, 18 MΩ cm).

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  • 94
    Millipore anti dcc
    Spine density and presynaptic release properties of axotomized neurons treated with exogenous netrin-1. a Representative dendrites before and 48 h post-axotomy treated with vehicle (HBS) or netrin-1 (Ntn1) beginning at 40 h post-axotomy (inverted fluorescence). Arrows : new spines; red asterisks : eliminated spines. Scale bars , 10 µm. b Quantification of spine density illustrated in a . Axotomy : n = 33 dendrites; 7 neurons; #spines/TDL: 392/3696 µm ( before ), 263/3447 µm ( after ). Axotomy + netrin-1 : n = 29 dendrites, 6 neurons; #spines/TDL: 293/3281 µm ( before ), 363/3417 µm ( after ). c Percent responsive FM puncta per neuron field at 48 h post-axotomy with HBS or netrin-1. n = 8–11 fields/chambers per condition over 5 experiments. d Number of responsive and unresponsive FM puncta from c . Significantly fewer unresponsive puncta followed axotomy compared to uninjured control (HBS). e , f Number of vGLUT1 and <t>vGAT</t> puncta per neuron area (uninjured control, axotomized + HBS, or axotomized + netrin-1) at 14 DIV. n = 8–9 neurons; 3 chambers per condition over 3 experiments. g Representative <t>DCC</t> immunostaining ( turquoise ) in uninjured control, post-axotomy, and post-axotomy + netrin-1 in cultures with similar spine densities. Neurons were retrogradely labeled with fluorescent protein (FP, magenta ) using an mCherry modified rabies virus. Scale bar , 10 µm. h Quantification of DCC immunofluorescence per spine region-of-interest (ROI). ROI: 2 µm diameter circular region surrounding each spine. Control, n = 295 ROIs; axotomy, n = 293 ROIs; axotomy + Ntn1, n = 210 ROIs. 8 neuron fields/3 chambers per condition; 3 experiments. i Quantification of spine density following 24 h of control antibody (IgG ab.) or DCC function blocking antibody (DCC ab.). IgG: n = 33 dendrites; 8 neurons; #spines/TDL: 464/4864 µm (before), 419/4712 µm (after). DCC ab: n = 34 dendrites; 7 neurons; #spines/TDL: 404/4433 µm (before), 222/3647 µm (after). j Representative FP-labeled dendritic segments immunostained for vGAT (inverted) and vGLUT1 (inverted) following 24 h application of IgG or DCC antibodies (outlined dendrites, solid magenta line ). Neurons were fixed at 15–16 DIV, older than the cultures in e , f . Scale bar , 10 µm. Quantification shown on the right. n = 23 neuron fields per condition; 3 chambers per condition over 3 experiments. b , i Repeated-measure two-way ANOVA, Bonferroni post hoc test; analyses included 1 chamber per condition for 3 experiments. c , j Unpaired two-tailed t -test. d – f , h One-way ANOVA, Bonferroni post hoc test. Error bars , s.e.m. * p
    Anti Dcc, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore human tmod1 peptide
    Decreased active force production and stiffness in skinned skeletal muscle fibers from <t>Tmod1</t> −/− mice. Shown are typical peak force-length change ( A ), relative stiffness-relative force ( B ) and y 0 -force ( C ) relationships of skinned muscle fibers from Tmod1 +/+ (solid circles) and Tmod1 −/− (open circles) mice.
    Human Tmod1 Peptide, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 2 phenylethanol
    Box plots showing the effect of different doses of the test compounds on the time that R. prolixus spent in the proximity of a vertebrate host when the insects were exposed to 2-mercaptoethanol, dimethyl sulfide, <t>2-phenylethanol,</t> and DEET (median, maximum and minimum values are shown). Letters denote significant differences among treatments according to Dunn’s multiple comparison test (p
    2 Phenylethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Spine density and presynaptic release properties of axotomized neurons treated with exogenous netrin-1. a Representative dendrites before and 48 h post-axotomy treated with vehicle (HBS) or netrin-1 (Ntn1) beginning at 40 h post-axotomy (inverted fluorescence). Arrows : new spines; red asterisks : eliminated spines. Scale bars , 10 µm. b Quantification of spine density illustrated in a . Axotomy : n = 33 dendrites; 7 neurons; #spines/TDL: 392/3696 µm ( before ), 263/3447 µm ( after ). Axotomy + netrin-1 : n = 29 dendrites, 6 neurons; #spines/TDL: 293/3281 µm ( before ), 363/3417 µm ( after ). c Percent responsive FM puncta per neuron field at 48 h post-axotomy with HBS or netrin-1. n = 8–11 fields/chambers per condition over 5 experiments. d Number of responsive and unresponsive FM puncta from c . Significantly fewer unresponsive puncta followed axotomy compared to uninjured control (HBS). e , f Number of vGLUT1 and vGAT puncta per neuron area (uninjured control, axotomized + HBS, or axotomized + netrin-1) at 14 DIV. n = 8–9 neurons; 3 chambers per condition over 3 experiments. g Representative DCC immunostaining ( turquoise ) in uninjured control, post-axotomy, and post-axotomy + netrin-1 in cultures with similar spine densities. Neurons were retrogradely labeled with fluorescent protein (FP, magenta ) using an mCherry modified rabies virus. Scale bar , 10 µm. h Quantification of DCC immunofluorescence per spine region-of-interest (ROI). ROI: 2 µm diameter circular region surrounding each spine. Control, n = 295 ROIs; axotomy, n = 293 ROIs; axotomy + Ntn1, n = 210 ROIs. 8 neuron fields/3 chambers per condition; 3 experiments. i Quantification of spine density following 24 h of control antibody (IgG ab.) or DCC function blocking antibody (DCC ab.). IgG: n = 33 dendrites; 8 neurons; #spines/TDL: 464/4864 µm (before), 419/4712 µm (after). DCC ab: n = 34 dendrites; 7 neurons; #spines/TDL: 404/4433 µm (before), 222/3647 µm (after). j Representative FP-labeled dendritic segments immunostained for vGAT (inverted) and vGLUT1 (inverted) following 24 h application of IgG or DCC antibodies (outlined dendrites, solid magenta line ). Neurons were fixed at 15–16 DIV, older than the cultures in e , f . Scale bar , 10 µm. Quantification shown on the right. n = 23 neuron fields per condition; 3 chambers per condition over 3 experiments. b , i Repeated-measure two-way ANOVA, Bonferroni post hoc test; analyses included 1 chamber per condition for 3 experiments. c , j Unpaired two-tailed t -test. d – f , h One-way ANOVA, Bonferroni post hoc test. Error bars , s.e.m. * p

    Journal: Nature Communications

    Article Title: Distal axotomy enhances retrograde presynaptic excitability onto injured pyramidal neurons via trans-synaptic signaling

    doi: 10.1038/s41467-017-00652-y

    Figure Lengend Snippet: Spine density and presynaptic release properties of axotomized neurons treated with exogenous netrin-1. a Representative dendrites before and 48 h post-axotomy treated with vehicle (HBS) or netrin-1 (Ntn1) beginning at 40 h post-axotomy (inverted fluorescence). Arrows : new spines; red asterisks : eliminated spines. Scale bars , 10 µm. b Quantification of spine density illustrated in a . Axotomy : n = 33 dendrites; 7 neurons; #spines/TDL: 392/3696 µm ( before ), 263/3447 µm ( after ). Axotomy + netrin-1 : n = 29 dendrites, 6 neurons; #spines/TDL: 293/3281 µm ( before ), 363/3417 µm ( after ). c Percent responsive FM puncta per neuron field at 48 h post-axotomy with HBS or netrin-1. n = 8–11 fields/chambers per condition over 5 experiments. d Number of responsive and unresponsive FM puncta from c . Significantly fewer unresponsive puncta followed axotomy compared to uninjured control (HBS). e , f Number of vGLUT1 and vGAT puncta per neuron area (uninjured control, axotomized + HBS, or axotomized + netrin-1) at 14 DIV. n = 8–9 neurons; 3 chambers per condition over 3 experiments. g Representative DCC immunostaining ( turquoise ) in uninjured control, post-axotomy, and post-axotomy + netrin-1 in cultures with similar spine densities. Neurons were retrogradely labeled with fluorescent protein (FP, magenta ) using an mCherry modified rabies virus. Scale bar , 10 µm. h Quantification of DCC immunofluorescence per spine region-of-interest (ROI). ROI: 2 µm diameter circular region surrounding each spine. Control, n = 295 ROIs; axotomy, n = 293 ROIs; axotomy + Ntn1, n = 210 ROIs. 8 neuron fields/3 chambers per condition; 3 experiments. i Quantification of spine density following 24 h of control antibody (IgG ab.) or DCC function blocking antibody (DCC ab.). IgG: n = 33 dendrites; 8 neurons; #spines/TDL: 464/4864 µm (before), 419/4712 µm (after). DCC ab: n = 34 dendrites; 7 neurons; #spines/TDL: 404/4433 µm (before), 222/3647 µm (after). j Representative FP-labeled dendritic segments immunostained for vGAT (inverted) and vGLUT1 (inverted) following 24 h application of IgG or DCC antibodies (outlined dendrites, solid magenta line ). Neurons were fixed at 15–16 DIV, older than the cultures in e , f . Scale bar , 10 µm. Quantification shown on the right. n = 23 neuron fields per condition; 3 chambers per condition over 3 experiments. b , i Repeated-measure two-way ANOVA, Bonferroni post hoc test; analyses included 1 chamber per condition for 3 experiments. c , j Unpaired two-tailed t -test. d – f , h One-way ANOVA, Bonferroni post hoc test. Error bars , s.e.m. * p

    Article Snippet: Coverslips were incubated with anti-MAP2 (1:1000; Millipore # AB5622), anti-beta tubulin III (1:2000; Aves #TUJ), anti-GAD67 (1:2000; Aves labs # GAD), anti-vGLUT1 (1:100; NeuroMab, clone N28/9, cat. #75-066), anti-vGAT (1:1000; Synaptic Systems #131 003), anti-DCC (1:100; Calbiochem #OP45), or anti-synapsin1 (1:500; Calbiochem #574778) primary antibodies in 1% blocking solution for overnight at 4 °C.

    Techniques: Fluorescence, Droplet Countercurrent Chromatography, Immunostaining, Labeling, Modification, Immunofluorescence, Blocking Assay, Two Tailed Test

    Netrin-1 triggers the dissociation of a DSCAM/DCC complex. (A) DSCAM (red) and DCC (green) proteins co-localize in precrossing commissural axons in transverse section of the E12 rat spinal cord. Lower panels are close ups (20x) of the ventral spinal cord

    Journal:

    Article Title: DSCAM is a netrin receptor that collaborates with DCC in mediating turning responses to netrin-1

    doi: 10.1016/j.cell.2008.05.030

    Figure Lengend Snippet: Netrin-1 triggers the dissociation of a DSCAM/DCC complex. (A) DSCAM (red) and DCC (green) proteins co-localize in precrossing commissural axons in transverse section of the E12 rat spinal cord. Lower panels are close ups (20x) of the ventral spinal cord

    Article Snippet: Anti-DCC (AF5, Calbiochem; 2 μg/ml), DSCAM-Fc or Fc was added to the medium 30 min before initiating the gradient.

    Techniques: Droplet Countercurrent Chromatography

    DSCAM binds netrin-1. (A) COS cells expressing DSCAM or DCC, but not Robo1 bind netrin-1 (top). Receptor expression was verified by immunocytochemistry (bottom). (B–D) Netrin-1 specifically binds to DSCAM. (B) DSCAMecto-Fc or control [(BSA)] protein

    Journal:

    Article Title: DSCAM is a netrin receptor that collaborates with DCC in mediating turning responses to netrin-1

    doi: 10.1016/j.cell.2008.05.030

    Figure Lengend Snippet: DSCAM binds netrin-1. (A) COS cells expressing DSCAM or DCC, but not Robo1 bind netrin-1 (top). Receptor expression was verified by immunocytochemistry (bottom). (B–D) Netrin-1 specifically binds to DSCAM. (B) DSCAMecto-Fc or control [(BSA)] protein

    Article Snippet: Anti-DCC (AF5, Calbiochem; 2 μg/ml), DSCAM-Fc or Fc was added to the medium 30 min before initiating the gradient.

    Techniques: Expressing, Droplet Countercurrent Chromatography, Immunocytochemistry

    DSCAM guides growth cones independent of DCC

    Journal:

    Article Title: DSCAM is a netrin receptor that collaborates with DCC in mediating turning responses to netrin-1

    doi: 10.1016/j.cell.2008.05.030

    Figure Lengend Snippet: DSCAM guides growth cones independent of DCC

    Article Snippet: Anti-DCC (AF5, Calbiochem; 2 μg/ml), DSCAM-Fc or Fc was added to the medium 30 min before initiating the gradient.

    Techniques: Droplet Countercurrent Chromatography

    Decreased active force production and stiffness in skinned skeletal muscle fibers from Tmod1 −/− mice. Shown are typical peak force-length change ( A ), relative stiffness-relative force ( B ) and y 0 -force ( C ) relationships of skinned muscle fibers from Tmod1 +/+ (solid circles) and Tmod1 −/− (open circles) mice.

    Journal: The FASEB Journal

    Article Title: Pointed-end capping by tropomodulin modulates actomyosin crossbridge formation in skeletal muscle fibers

    doi: 10.1096/fj.13-239640

    Figure Lengend Snippet: Decreased active force production and stiffness in skinned skeletal muscle fibers from Tmod1 −/− mice. Shown are typical peak force-length change ( A ), relative stiffness-relative force ( B ) and y 0 -force ( C ) relationships of skinned muscle fibers from Tmod1 +/+ (solid circles) and Tmod1 −/− (open circles) mice.

    Article Snippet: For Western blotting, primary antibodies were rabbit polyclonal antiserum to residues 340–359 of a human Tmod1 peptide (PA2211, 1:5000; ref. ), rabbit polyclonal antiserum to chicken Tmod4 preadsorbed by passage through a Tmod1 Sepharose column (R3577bl3c, 1:2500; ref. ), mouse monoclonal anti-actin (C4, 1:10000; EMD Millipore, Billerica, MA, USA), and mouse monoclonal anti-tropomyosin (CH1, 1:500; Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Mouse Assay

    Box plots showing the effect of different doses of the test compounds on the time that R. prolixus spent in the proximity of a vertebrate host when the insects were exposed to 2-mercaptoethanol, dimethyl sulfide, 2-phenylethanol, and DEET (median, maximum and minimum values are shown). Letters denote significant differences among treatments according to Dunn’s multiple comparison test (p

    Journal: bioRxiv

    Article Title: Novel repellents for the blood-sucking insects Rhodnius prolixus and Triatoma infestans, vectors of Chagas disease

    doi: 10.1101/789602

    Figure Lengend Snippet: Box plots showing the effect of different doses of the test compounds on the time that R. prolixus spent in the proximity of a vertebrate host when the insects were exposed to 2-mercaptoethanol, dimethyl sulfide, 2-phenylethanol, and DEET (median, maximum and minimum values are shown). Letters denote significant differences among treatments according to Dunn’s multiple comparison test (p

    Article Snippet: Dimethyl sulfide and 2-mercaptoethanol solutions were made in distilled water, while ethanol was the solvent for 2-phenylethanol and DEET.

    Techniques: