phenylethanol  (Millipore)

 
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    Name:
    2 Phenylethanol
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    77861
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    Structured Review

    Millipore phenylethanol
    2 Phenylethanol

    https://www.bioz.com/result/phenylethanol/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylethanol - by Bioz Stars, 2021-05
    95/100 stars

    Images

    1) Product Images from "Secondary metabolites from food-derived yeasts inhibit virulence of Candida albicans"

    Article Title: Secondary metabolites from food-derived yeasts inhibit virulence of Candida albicans

    Journal: bioRxiv

    doi: 10.1101/2020.08.14.251447

    S. cerevisiae mutant analysis shows phenylethanol and tryptophol are required for beneficial activity in vitro and in vivo. (A) The neutralized secretome of aro8 aro9 double mutant (grey bar), that does not produce aromatic alcohols, was tested for its ability to prevent adhesion of C. albicans , compared to its isogenic wild type counterpart (white bar) or untreated controls (black bars), (n=4 assay replicates). (B.) Life span of C. elegans treated either with aro8 aro9 double mutant or its wild type counterpart. The double mutant (red curve) does not protect the nematode as well as its isogenic wild type control strain (blue curve) (n= 4 experimental replicates, 163 ± 11 nematodes). (C) Working model for the mechanism by which beneficial yeasts function to inhibit C. albicans virulence. Error bars represents means± standard deviations (SD). Kaplan-Meier statistical analysis tools by Log-rank (Mantel-Cox) tests were used for the C. elegans survival assay and statistical significance is expressed with respect to control.
    Figure Legend Snippet: S. cerevisiae mutant analysis shows phenylethanol and tryptophol are required for beneficial activity in vitro and in vivo. (A) The neutralized secretome of aro8 aro9 double mutant (grey bar), that does not produce aromatic alcohols, was tested for its ability to prevent adhesion of C. albicans , compared to its isogenic wild type counterpart (white bar) or untreated controls (black bars), (n=4 assay replicates). (B.) Life span of C. elegans treated either with aro8 aro9 double mutant or its wild type counterpart. The double mutant (red curve) does not protect the nematode as well as its isogenic wild type control strain (blue curve) (n= 4 experimental replicates, 163 ± 11 nematodes). (C) Working model for the mechanism by which beneficial yeasts function to inhibit C. albicans virulence. Error bars represents means± standard deviations (SD). Kaplan-Meier statistical analysis tools by Log-rank (Mantel-Cox) tests were used for the C. elegans survival assay and statistical significance is expressed with respect to control.

    Techniques Used: Mutagenesis, Activity Assay, In Vitro, In Vivo, Clonogenic Cell Survival Assay

    Phenylethanol and tryptophol are sufficient for beneficial activity in vitro and in vivo. (A) Varying concentration (100, 300 and 500 μM) of commercially procured aromatic alcohols, phenylethanol and tryptophol were exposed to C. albicans and biomass was measured with crystal violet (n = 5 experimental replicates). (B) Life span of C. elegans infected with C. albicans treated tryptophol (100 μM) (green curve) and phenylethanol (100 μM) (red curve) (n =3 experimental replicates, 82 ± 28 nematodes). (C) Representative images showing filamentation of C. albicans , upon phenylethanol and tryptophol treatment. Error bars represents means± standard deviations (SD). Kaplan-Meier statistical analysis tools by Log-rank (Mantel-Cox) tests were used for the C. elegans survival assay.
    Figure Legend Snippet: Phenylethanol and tryptophol are sufficient for beneficial activity in vitro and in vivo. (A) Varying concentration (100, 300 and 500 μM) of commercially procured aromatic alcohols, phenylethanol and tryptophol were exposed to C. albicans and biomass was measured with crystal violet (n = 5 experimental replicates). (B) Life span of C. elegans infected with C. albicans treated tryptophol (100 μM) (green curve) and phenylethanol (100 μM) (red curve) (n =3 experimental replicates, 82 ± 28 nematodes). (C) Representative images showing filamentation of C. albicans , upon phenylethanol and tryptophol treatment. Error bars represents means± standard deviations (SD). Kaplan-Meier statistical analysis tools by Log-rank (Mantel-Cox) tests were used for the C. elegans survival assay.

    Techniques Used: Activity Assay, In Vitro, In Vivo, Concentration Assay, Infection, Clonogenic Cell Survival Assay

    2) Product Images from "Bacterial bifunctional chorismate mutase-prephenate dehydratase PheA increases flux into the yeast phenylalanine pathway and improves mandelic acid production"

    Article Title: Bacterial bifunctional chorismate mutase-prephenate dehydratase PheA increases flux into the yeast phenylalanine pathway and improves mandelic acid production

    Journal: Metabolic Engineering Communications

    doi: 10.1016/j.mec.2018.e00079

    Using the bifunctional E. coli enzyme PheA fbr for mandelic acid production in an aro7Δ S. cerevisiae strain. A) Growth test with MRY36 (CEN.PK2-1C TRP1 Shik↑ aro10Δ aro8Δ pdc5Δ aro7Δ ) cells harboring different plasmid combinations on SMD with and without supplementation of aromatic amino acids. Cell dilutions of OD 600 1, 10 −1 , 10 −2 and 10 −3 (from left to right, respectively) were used. The picture was taken after 3d at 30 °C. EV, empty vector. B), C) and D) Production of mandelic acid (MA, B), hydroxymandelic acid (HMA, C) and phenylacetic acid + phenylethanol (PAA+PET, D) by the aro7Δ strain MRY36 expressing either pheA fbr and hmaS (red) or ARO7 fbr , PHA2 and hmaS (blue). E) Fermentation with the aro7Δ strain MRY36 harboring the hmaS-pheA fbr plasmid MRV144. MA, glucose and ethanol titers and OD 600 in SMD without supplementation of aromatic amino acids. The fermentations were performed in SMD (20 g/L glucose) without supplementation aromatic amino acids and with a starting OD 600 of 5. Error bars indicate standard deviation of biological duplicates (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).
    Figure Legend Snippet: Using the bifunctional E. coli enzyme PheA fbr for mandelic acid production in an aro7Δ S. cerevisiae strain. A) Growth test with MRY36 (CEN.PK2-1C TRP1 Shik↑ aro10Δ aro8Δ pdc5Δ aro7Δ ) cells harboring different plasmid combinations on SMD with and without supplementation of aromatic amino acids. Cell dilutions of OD 600 1, 10 −1 , 10 −2 and 10 −3 (from left to right, respectively) were used. The picture was taken after 3d at 30 °C. EV, empty vector. B), C) and D) Production of mandelic acid (MA, B), hydroxymandelic acid (HMA, C) and phenylacetic acid + phenylethanol (PAA+PET, D) by the aro7Δ strain MRY36 expressing either pheA fbr and hmaS (red) or ARO7 fbr , PHA2 and hmaS (blue). E) Fermentation with the aro7Δ strain MRY36 harboring the hmaS-pheA fbr plasmid MRV144. MA, glucose and ethanol titers and OD 600 in SMD without supplementation of aromatic amino acids. The fermentations were performed in SMD (20 g/L glucose) without supplementation aromatic amino acids and with a starting OD 600 of 5. Error bars indicate standard deviation of biological duplicates (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).

    Techniques Used: Plasmid Preparation, Positron Emission Tomography, Expressing, Standard Deviation

    Applied modifications of the aromatic amino acid pathway of S. cerevisiae for mandelic acid production. The heterologous hydroxymandelate synthase (HmaS, red) converts the intermediates of the aromatic amino acid pathway phenylpyruvate (PPY) and hydroxyphenylpyruvate (HPP) to mandelic acid (MA) and hydroxymandelic acid (HMA), respectively. Enzymes that were targeted to mitochondria/peroxisomes in our compartmentalization approach are marked with a star. The compartment (mitochondrion or peroxisome) containing the three consecutive enzymes Aro7 fbr , Pha2 and HmaS is depicted as a grey area surrounded by a dashed line. The bifunctional E. coli enzyme PheA fbr is indicated in orange (CM, chorismate mutase; PDT, prephenate dehydratase). Dashed arrows indicate multiple enzymatic steps. Enzymes whose genes were deleted in a part of the strains used in this work are labeled in grey. ANTH, anthranilate; Trp, tryptophan; PAC, phenylacetaldehyde; pPAC, p -hydroxyphenylacetaldehyde; Phe, phenylalanine; Tyr, tyrosine; PAA, phenylacetic acid; pPAA, p -hydroxyphenylacetic acid; PET, phenylethanol; pPET, p -hydroxyphenylethanol.
    Figure Legend Snippet: Applied modifications of the aromatic amino acid pathway of S. cerevisiae for mandelic acid production. The heterologous hydroxymandelate synthase (HmaS, red) converts the intermediates of the aromatic amino acid pathway phenylpyruvate (PPY) and hydroxyphenylpyruvate (HPP) to mandelic acid (MA) and hydroxymandelic acid (HMA), respectively. Enzymes that were targeted to mitochondria/peroxisomes in our compartmentalization approach are marked with a star. The compartment (mitochondrion or peroxisome) containing the three consecutive enzymes Aro7 fbr , Pha2 and HmaS is depicted as a grey area surrounded by a dashed line. The bifunctional E. coli enzyme PheA fbr is indicated in orange (CM, chorismate mutase; PDT, prephenate dehydratase). Dashed arrows indicate multiple enzymatic steps. Enzymes whose genes were deleted in a part of the strains used in this work are labeled in grey. ANTH, anthranilate; Trp, tryptophan; PAC, phenylacetaldehyde; pPAC, p -hydroxyphenylacetaldehyde; Phe, phenylalanine; Tyr, tyrosine; PAA, phenylacetic acid; pPAA, p -hydroxyphenylacetic acid; PET, phenylethanol; pPET, p -hydroxyphenylethanol.

    Techniques Used: Labeling, Positron Emission Tomography

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  • 93
    Millipore salbutamol
    Effects of <t>salbutamol</t> and roflumilast on macrophages. Macrophages were pre‐incubated (30 min) with indomethacin (1 μM) and then with or without either (A) salbutamol, (B) roflumilast or (C) PGE 2 in the absence (control) or presence of a single concentration of roflumilast (30 nM) for 30 min before challenge with LPS (1 ng·mL −1 ) for 22 h after which TNF‐α was measured in the supernatants. The horizontal grid line in (C) shows the inhibition seen with roflumilast alone (22 ± 5% inhibition). Results are expressed as the % inhibition of the unblocked control TNF‐α releases, which ranged from 2363 ± 835 to 2208 ± 969 pg·mL −1 . Data shown are means ± SEM for five (A, B and C) experiments.
    Salbutamol, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/salbutamol/product/Millipore
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    Effects of salbutamol and roflumilast on macrophages. Macrophages were pre‐incubated (30 min) with indomethacin (1 μM) and then with or without either (A) salbutamol, (B) roflumilast or (C) PGE 2 in the absence (control) or presence of a single concentration of roflumilast (30 nM) for 30 min before challenge with LPS (1 ng·mL −1 ) for 22 h after which TNF‐α was measured in the supernatants. The horizontal grid line in (C) shows the inhibition seen with roflumilast alone (22 ± 5% inhibition). Results are expressed as the % inhibition of the unblocked control TNF‐α releases, which ranged from 2363 ± 835 to 2208 ± 969 pg·mL −1 . Data shown are means ± SEM for five (A, B and C) experiments.

    Journal: British Journal of Pharmacology

    Article Title: The anti‐inflammatory effects of PGE2 on human lung macrophages are mediated by the EP4 receptor) The anti‐inflammatory effects of PGE2 on human lung macrophages are mediated by the EP4 receptor

    doi: 10.1111/bph.13565

    Figure Lengend Snippet: Effects of salbutamol and roflumilast on macrophages. Macrophages were pre‐incubated (30 min) with indomethacin (1 μM) and then with or without either (A) salbutamol, (B) roflumilast or (C) PGE 2 in the absence (control) or presence of a single concentration of roflumilast (30 nM) for 30 min before challenge with LPS (1 ng·mL −1 ) for 22 h after which TNF‐α was measured in the supernatants. The horizontal grid line in (C) shows the inhibition seen with roflumilast alone (22 ± 5% inhibition). Results are expressed as the % inhibition of the unblocked control TNF‐α releases, which ranged from 2363 ± 835 to 2208 ± 969 pg·mL −1 . Data shown are means ± SEM for five (A, B and C) experiments.

    Article Snippet: The materials used were supplied as follows: indomethacin, PGE2 , Percoll, salbutamol, Tri‐Reagent (all Sigma); gentamicin, penicillin/streptomycin, fungizone, RPMI 1640, (Invitrogen, Paisley, UK); butaprost, misoprostol, L‐902,688 (Cayman Chemical Company); L‐161,982 (Tocris Bioscience, Bristol, UK); roflumilast (Santa Cruz Biotechnology, Heidelberg, Germany); Quick‐Diff (Reagena, Toivala, Finland); FCS (Promocell, Heidelberg, Germany); and LPS (Enzo Life Sciences, Exeter, UK).

    Techniques: Incubation, Concentration Assay, Inhibition

    The effect of NS309, salbutamol and GEA3175 in (A–C) human pulmonary arteries with or without endothelium, (D–F) human bronchioles with or without epithelium. Data points represent means ± SEM, with n = 4–7 per data point.

    Journal: British Journal of Pharmacology

    Article Title: Activation of endothelial and epithelial KCa2.3 calcium-activated potassium channels by NS309 relaxes human small pulmonary arteries and bronchioles

    doi: 10.1111/j.1476-5381.2012.01986.x

    Figure Lengend Snippet: The effect of NS309, salbutamol and GEA3175 in (A–C) human pulmonary arteries with or without endothelium, (D–F) human bronchioles with or without epithelium. Data points represent means ± SEM, with n = 4–7 per data point.

    Article Snippet: Concentration–response curves (0.001–10 µM) were constructed for the KCa 2.3 and KCa 3.1 channel opener NS309 (Neurosearch A/S, Ballerup, Denmark), the ‘classical’ KCa 2.3 and KCa 3.1 channel opener 1-ethyl-benzimidazolinone (1-EBIO; Sigma Aldrich, St. Louis, MO); a selective activator of KCa 2 channels, cyclohexyl-[2-(3,5-dimethyl-pyrazol-1-yl)-6-methyl-pyrimidin-4-yl]-amine (CyPPA); a NO donor, 3-(2-chloro-3-methylphenyl)-5-[[(4-methylphenyl)sulphonyl] amino]-hydroxide (GEA 3175; GEA A/S, Copenhagen, Denmark) and the β2 -adrenoceptor agonist, salbutamol (Sigma Aldrich).

    Techniques:

    The effect of vehicle, NS309, 1-EBIO, levcromakalim, salbutamol and GEA 3175 in (A, B) human pulmonary arteries and (C, D) human bronchioles. Data points represent means ± SEM, with n = 4–12 per data point.

    Journal: British Journal of Pharmacology

    Article Title: Activation of endothelial and epithelial KCa2.3 calcium-activated potassium channels by NS309 relaxes human small pulmonary arteries and bronchioles

    doi: 10.1111/j.1476-5381.2012.01986.x

    Figure Lengend Snippet: The effect of vehicle, NS309, 1-EBIO, levcromakalim, salbutamol and GEA 3175 in (A, B) human pulmonary arteries and (C, D) human bronchioles. Data points represent means ± SEM, with n = 4–12 per data point.

    Article Snippet: Concentration–response curves (0.001–10 µM) were constructed for the KCa 2.3 and KCa 3.1 channel opener NS309 (Neurosearch A/S, Ballerup, Denmark), the ‘classical’ KCa 2.3 and KCa 3.1 channel opener 1-ethyl-benzimidazolinone (1-EBIO; Sigma Aldrich, St. Louis, MO); a selective activator of KCa 2 channels, cyclohexyl-[2-(3,5-dimethyl-pyrazol-1-yl)-6-methyl-pyrimidin-4-yl]-amine (CyPPA); a NO donor, 3-(2-chloro-3-methylphenyl)-5-[[(4-methylphenyl)sulphonyl] amino]-hydroxide (GEA 3175; GEA A/S, Copenhagen, Denmark) and the β2 -adrenoceptor agonist, salbutamol (Sigma Aldrich).

    Techniques:

    Effects of isoprenaline, salbutamol and SR 59104A on pulmonary reactivity; influence of K+ channel, EDRF and cyclo-oxygenase inhibitors

    Journal: British Journal of Pharmacology

    Article Title: Role of potassium channels and nitric oxide in the relaxant effects elicited by ?-adrenoceptor agonists on hypoxic vasoconstriction in the isolated perfused lung of the rat

    doi: 10.1038/sj.bjp.0702575

    Figure Lengend Snippet: Effects of isoprenaline, salbutamol and SR 59104A on pulmonary reactivity; influence of K+ channel, EDRF and cyclo-oxygenase inhibitors

    Article Snippet: The drugs used were: SR 59104A (N- [6 hydroxy-1,2,3,4-tetrahydronaphtalen-(2 R )-2yl) methyl]-(2 R )-2hydroxy-2-(3-chlorophenyl) ethanamine hydrochloride (Sanofi-Midy, Research Centre, Milan, Italy), glibenclamide (Laboratoire Hoechst, Paris la Défense, France), isoprenaline, salbutamol, angiotensin II, NG -nitro- L -arginine methyl ester, indomethacin (Sigma Chimie, La Verpillère, France) and charybdotoxin and apamin (Latoxan, Rosans, France).

    Techniques:

    Effects of isoprenaline, salbutamol and SR 59104A on pulmonary reactivity; influence of K+ channel, EDRF and cyclo-oxygenase inhibitors

    Journal: British Journal of Pharmacology

    Article Title: Role of potassium channels and nitric oxide in the relaxant effects elicited by ?-adrenoceptor agonists on hypoxic vasoconstriction in the isolated perfused lung of the rat

    doi: 10.1038/sj.bjp.0702575

    Figure Lengend Snippet: Effects of isoprenaline, salbutamol and SR 59104A on pulmonary reactivity; influence of K+ channel, EDRF and cyclo-oxygenase inhibitors

    Article Snippet: The drugs used were: SR 59104A (N- [6 hydroxy-1,2,3,4-tetrahydronaphtalen-(2 R )-2yl) methyl]-(2 R )-2hydroxy-2-(3-chlorophenyl) ethanamine hydrochloride (Sanofi-Midy, Research Centre, Milan, Italy), glibenclamide (Laboratoire Hoechst, Paris la Défense, France), isoprenaline, salbutamol, angiotensin II, NG -nitro- L -arginine methyl ester, indomethacin (Sigma Chimie, La Verpillère, France) and charybdotoxin and apamin (Latoxan, Rosans, France).

    Techniques:

    Brown adipose tissue (BAT) blood flow estimated by contrast-ultrasound before and during β2-adrenoreceptor stimulation. Individual data of Aβ product at baseline and 10 min after the onset of salbutamol infusion at 0.2 μg·kg

    Journal: Journal of Applied Physiology

    Article Title: Relationship of brown adipose tissue perfusion and function: a study through β2-adrenoreceptor stimulation

    doi: 10.1152/japplphysiol.00634.2015

    Figure Lengend Snippet: Brown adipose tissue (BAT) blood flow estimated by contrast-ultrasound before and during β2-adrenoreceptor stimulation. Individual data of Aβ product at baseline and 10 min after the onset of salbutamol infusion at 0.2 μg·kg

    Article Snippet: Salbutamol was obtained from Sigma Aldrich (S8260) and diluted in sterile 0.9% saline before injection.

    Techniques: Flow Cytometry

    BAT ( A ) and visceral ( B ) and subcutaneous white adipose tissue (WAT; C ) glucose uptake after β2-adrenoreceptor stimulation. Mice were treated with 10 μg/g mouse body wt of salbutamol intraperitoneally ( n = 10), and their glucose uptake

    Journal: Journal of Applied Physiology

    Article Title: Relationship of brown adipose tissue perfusion and function: a study through β2-adrenoreceptor stimulation

    doi: 10.1152/japplphysiol.00634.2015

    Figure Lengend Snippet: BAT ( A ) and visceral ( B ) and subcutaneous white adipose tissue (WAT; C ) glucose uptake after β2-adrenoreceptor stimulation. Mice were treated with 10 μg/g mouse body wt of salbutamol intraperitoneally ( n = 10), and their glucose uptake

    Article Snippet: Salbutamol was obtained from Sigma Aldrich (S8260) and diluted in sterile 0.9% saline before injection.

    Techniques: Mouse Assay