forskolin  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Forskolin
    Description:
    Forskolin is a cell permeable diterpenoid isolated from Coleus forskohlii
    Catalog Number:
    f3917
    Price:
    None
    Buy from Supplier


    Structured Review

    Millipore forskolin
    Forskolin
    Forskolin is a cell permeable diterpenoid isolated from Coleus forskohlii
    https://www.bioz.com/result/forskolin/product/Millipore
    Average 99 stars, based on 1215 article reviews
    Price from $9.99 to $1999.99
    forskolin - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Neurotrophic Effect of Citrus 5-Hydroxy-3,6,7,8,3?,4?-Hexamethoxyflavone: Promotion of Neurite Outgrowth via cAMP/PKA/CREB Pathway in PC12 Cells"

    Article Title: Neurotrophic Effect of Citrus 5-Hydroxy-3,6,7,8,3?,4?-Hexamethoxyflavone: Promotion of Neurite Outgrowth via cAMP/PKA/CREB Pathway in PC12 Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0028280

    Effects of the molecular inhibitors on the 5-OH-HxMF -mediated CREB activation. ( A ) PC12 cells were transfected with a CRE-mediated luciferase reporter construct and Renilla luciferase control plasmid for 24 h. Following transfection, cells were pre-treated for 30 min with inhibitors 10 µM U0126, 2.5 µM BIM, 20 µM LY294002, 10 µM KN-62, 500 µM SQ22536, 10 µM H-89 or vehicle (0.1% DMSO), followed by exposure to 5-OH-HxMF (20 µM) for 8 h. Forskolin (2 µM)-treated transfected cells as a positive control for CRE-luciferase reporter assay. The intensity of the luciferase reactions measured in the lysates of the transfectants was normalized to their Renilla luciferase control activity. ( B ) PC12 cells were seeded on poly-L-lysine-coated 100 mm dishes in normal medium for 24 h and then shifted to low serum medium (1% HS and 0.5% FBS) for further 24 h culture. Cells were treated with inhibitor SQ22536 or H-89 for 30 min prior to exposure of vehicle (0.1% DMSO) or 5-OH-HxMF (20 µM) for 60 min. Phosphor-CREB (p-CREB) and CREB were analyzed by Western blotting as described in Materials and Methods . The immunoblot experiments were replicated at least three times and a representative blot was shown. Normalized intensity of p-CREB versus CREB is presented as the mean ± SD of three independent experiments. ** p
    Figure Legend Snippet: Effects of the molecular inhibitors on the 5-OH-HxMF -mediated CREB activation. ( A ) PC12 cells were transfected with a CRE-mediated luciferase reporter construct and Renilla luciferase control plasmid for 24 h. Following transfection, cells were pre-treated for 30 min with inhibitors 10 µM U0126, 2.5 µM BIM, 20 µM LY294002, 10 µM KN-62, 500 µM SQ22536, 10 µM H-89 or vehicle (0.1% DMSO), followed by exposure to 5-OH-HxMF (20 µM) for 8 h. Forskolin (2 µM)-treated transfected cells as a positive control for CRE-luciferase reporter assay. The intensity of the luciferase reactions measured in the lysates of the transfectants was normalized to their Renilla luciferase control activity. ( B ) PC12 cells were seeded on poly-L-lysine-coated 100 mm dishes in normal medium for 24 h and then shifted to low serum medium (1% HS and 0.5% FBS) for further 24 h culture. Cells were treated with inhibitor SQ22536 or H-89 for 30 min prior to exposure of vehicle (0.1% DMSO) or 5-OH-HxMF (20 µM) for 60 min. Phosphor-CREB (p-CREB) and CREB were analyzed by Western blotting as described in Materials and Methods . The immunoblot experiments were replicated at least three times and a representative blot was shown. Normalized intensity of p-CREB versus CREB is presented as the mean ± SD of three independent experiments. ** p

    Techniques Used: Activation Assay, Transfection, Luciferase, Construct, Plasmid Preparation, Positive Control, Reporter Assay, Activity Assay, Western Blot

    2) Product Images from "High-content analysis of cancer-cell specific apoptosis and inhibition of in vivo angiogenesis by synthetic (-)-pironetin and analogs"

    Article Title: High-content analysis of cancer-cell specific apoptosis and inhibition of in vivo angiogenesis by synthetic (-)-pironetin and analogs

    Journal: Chemical biology & drug design

    doi: 10.1111/j.1747-0285.2009.00866.x

    Pironetin and 3 inhibit angiogenesis in zebrafish embryos Lateral views of 32hpf ( upper panel) or 48 hpf (lower panel) Tg(fli1:EGFP) y1 treated with compounds indicated. (A) In vehicle treated embryos, intersegmental vessels (ISV) sprout from the dorsal aorta (DA) and connect to the dorsal longitudinal anastomotic vessel (DLAV). (B) (−)-pironetin (2.5 μM) prevented the normal outgrowth of ISV and formation of the DLAV. (C) At 50μM 3 , ISV growth was stunted in a similar fashion to 2.5 μM (−)-pironetin. These phenotypes are similar to embryos treated with 40μM SU11652, a known VEGFR2 inhibitor (D) . Fluorescence images are shown inverted to improve visibility.
    Figure Legend Snippet: Pironetin and 3 inhibit angiogenesis in zebrafish embryos Lateral views of 32hpf ( upper panel) or 48 hpf (lower panel) Tg(fli1:EGFP) y1 treated with compounds indicated. (A) In vehicle treated embryos, intersegmental vessels (ISV) sprout from the dorsal aorta (DA) and connect to the dorsal longitudinal anastomotic vessel (DLAV). (B) (−)-pironetin (2.5 μM) prevented the normal outgrowth of ISV and formation of the DLAV. (C) At 50μM 3 , ISV growth was stunted in a similar fashion to 2.5 μM (−)-pironetin. These phenotypes are similar to embryos treated with 40μM SU11652, a known VEGFR2 inhibitor (D) . Fluorescence images are shown inverted to improve visibility.

    Techniques Used: Fluorescence

    (−)-Pironetin and 3 caused cancer-cell specific apoptosis and cell cycle arrest HeLa and IMR-90 cells (10,000 per well) were plated on the left and right halves, respectively, of a 384-well microplate. Wells were treated from right to left with 10 two-fold serial dilutions of drugs as shown. The two columns in the center of the plate show 14 replicates of vehicle treated control wells for each cell line. Starting concentrations for each agent are shown in parentheses. (A) Cell cycle arrest. Total Hoechst 33342 staining intensities from a minimum of 1,000 cells are presented as DNA content density distributions for each individual well on the microplate. Highlighted are the lowest concentrations where a shift in DNA content could be detected by visual inspection. (B) High-content analysis of apoptosis. HeLa, IMR-90, or A-549 cells treated with vehicle or ten two-fold dilutions of paclitaxel (□), vincristine (■), (−)-pironetin (●), 3 (○), or 4 (▲) were analyzed by high-content analysis for cell density, chromatin condensation, caspase cleavage, and p53 induction. Data are the averages of quadruplicate wells from a single experiment that has been repeated at least once with similar results. (HeLa, n=1).
    Figure Legend Snippet: (−)-Pironetin and 3 caused cancer-cell specific apoptosis and cell cycle arrest HeLa and IMR-90 cells (10,000 per well) were plated on the left and right halves, respectively, of a 384-well microplate. Wells were treated from right to left with 10 two-fold serial dilutions of drugs as shown. The two columns in the center of the plate show 14 replicates of vehicle treated control wells for each cell line. Starting concentrations for each agent are shown in parentheses. (A) Cell cycle arrest. Total Hoechst 33342 staining intensities from a minimum of 1,000 cells are presented as DNA content density distributions for each individual well on the microplate. Highlighted are the lowest concentrations where a shift in DNA content could be detected by visual inspection. (B) High-content analysis of apoptosis. HeLa, IMR-90, or A-549 cells treated with vehicle or ten two-fold dilutions of paclitaxel (□), vincristine (■), (−)-pironetin (●), 3 (○), or 4 (▲) were analyzed by high-content analysis for cell density, chromatin condensation, caspase cleavage, and p53 induction. Data are the averages of quadruplicate wells from a single experiment that has been repeated at least once with similar results. (HeLa, n=1).

    Techniques Used: Staining, High Content Screening

    Design and synthesis of pironetin analogs A. Covalent tubulin modification by αLys352 conjugate addition to bound (−)-pironetin and a pironetin derivative designed to mimic interaction with αLys352 without covalent binding. B , Synthesis of pironetin derivatives 3 and 4 .
    Figure Legend Snippet: Design and synthesis of pironetin analogs A. Covalent tubulin modification by αLys352 conjugate addition to bound (−)-pironetin and a pironetin derivative designed to mimic interaction with αLys352 without covalent binding. B , Synthesis of pironetin derivatives 3 and 4 .

    Techniques Used: Modification, Binding Assay

    Related Articles

    Activated Clotting Time Assay:

    Article Title: Stress-related hormone norepinephrine induces interleukin-6 expression in GES-1 cells
    Article Snippet: .. Reagents Phentolamine mesylate was purchased from Santa Cruz (USA), forskolin from Calbiochem (USA), KT5720 from Tocris (UK), and actinomycin D (Act D) from Beyotime Institute of Biotechnology Co. (China). ..

    other:

    Article Title: Involvement of the Notch pathway in terminal astrocytic differentiation: role of PKA
    Article Snippet: Reagents dbcAMP, H89 and Forskolin were all obtained from Sigma–Aldrich.

    Article Title: Cyclooxygenase-2 contributes to oxidopamine-mediated neuronal inflammation and injury via the prostaglandin E2 receptor EP2 subtype
    Article Snippet: 6-hydroxydopamine (6-OHDA), forskolin and rolipram were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Millipore pironetin
    <t>Pironetin</t> and 3 inhibit angiogenesis in zebrafish embryos Lateral views of 32hpf ( upper panel) or 48 hpf (lower panel) Tg(fli1:EGFP) y1 treated with compounds indicated. (A) In vehicle treated embryos, intersegmental vessels (ISV) sprout from the dorsal aorta (DA) and connect to the dorsal longitudinal anastomotic vessel (DLAV). (B) (−)-pironetin (2.5 μM) prevented the normal outgrowth of ISV and formation of the DLAV. (C) At 50μM 3 , ISV growth was stunted in a similar fashion to 2.5 μM (−)-pironetin. These phenotypes are similar to embryos treated with 40μM SU11652, a known VEGFR2 inhibitor (D) . Fluorescence images are shown inverted to improve visibility.
    Pironetin, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pironetin/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pironetin - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Pironetin and 3 inhibit angiogenesis in zebrafish embryos Lateral views of 32hpf ( upper panel) or 48 hpf (lower panel) Tg(fli1:EGFP) y1 treated with compounds indicated. (A) In vehicle treated embryos, intersegmental vessels (ISV) sprout from the dorsal aorta (DA) and connect to the dorsal longitudinal anastomotic vessel (DLAV). (B) (−)-pironetin (2.5 μM) prevented the normal outgrowth of ISV and formation of the DLAV. (C) At 50μM 3 , ISV growth was stunted in a similar fashion to 2.5 μM (−)-pironetin. These phenotypes are similar to embryos treated with 40μM SU11652, a known VEGFR2 inhibitor (D) . Fluorescence images are shown inverted to improve visibility.

    Journal: Chemical biology & drug design

    Article Title: High-content analysis of cancer-cell specific apoptosis and inhibition of in vivo angiogenesis by synthetic (-)-pironetin and analogs

    doi: 10.1111/j.1747-0285.2009.00866.x

    Figure Lengend Snippet: Pironetin and 3 inhibit angiogenesis in zebrafish embryos Lateral views of 32hpf ( upper panel) or 48 hpf (lower panel) Tg(fli1:EGFP) y1 treated with compounds indicated. (A) In vehicle treated embryos, intersegmental vessels (ISV) sprout from the dorsal aorta (DA) and connect to the dorsal longitudinal anastomotic vessel (DLAV). (B) (−)-pironetin (2.5 μM) prevented the normal outgrowth of ISV and formation of the DLAV. (C) At 50μM 3 , ISV growth was stunted in a similar fashion to 2.5 μM (−)-pironetin. These phenotypes are similar to embryos treated with 40μM SU11652, a known VEGFR2 inhibitor (D) . Fluorescence images are shown inverted to improve visibility.

    Article Snippet: Five transgenic zebrafish embryos ( Tg(Fli1:EGFP)y1 ) were then treated in 96-well plates in 200 μl E3 medium (5mM NaCl, 0.33mM CaCl2 , 0.17mM KCl, 0.33mM MgSO4 ) containing vehicle (DMSO, 0.5%), (−)- pironetin (5 μM to 0.5 μM), 3 (50μM) or SU11652 (Calbiochem) (40 μM) for 8 h or 24 h. The chorions were manually removed from treated embryos and single embryos were transferred to a 384-well plate containing 40μg/mL MS222 (tricaine methanesulfonate, Sigma) in E3 for imaging.

    Techniques: Fluorescence

    (−)-Pironetin and 3 caused cancer-cell specific apoptosis and cell cycle arrest HeLa and IMR-90 cells (10,000 per well) were plated on the left and right halves, respectively, of a 384-well microplate. Wells were treated from right to left with 10 two-fold serial dilutions of drugs as shown. The two columns in the center of the plate show 14 replicates of vehicle treated control wells for each cell line. Starting concentrations for each agent are shown in parentheses. (A) Cell cycle arrest. Total Hoechst 33342 staining intensities from a minimum of 1,000 cells are presented as DNA content density distributions for each individual well on the microplate. Highlighted are the lowest concentrations where a shift in DNA content could be detected by visual inspection. (B) High-content analysis of apoptosis. HeLa, IMR-90, or A-549 cells treated with vehicle or ten two-fold dilutions of paclitaxel (□), vincristine (■), (−)-pironetin (●), 3 (○), or 4 (▲) were analyzed by high-content analysis for cell density, chromatin condensation, caspase cleavage, and p53 induction. Data are the averages of quadruplicate wells from a single experiment that has been repeated at least once with similar results. (HeLa, n=1).

    Journal: Chemical biology & drug design

    Article Title: High-content analysis of cancer-cell specific apoptosis and inhibition of in vivo angiogenesis by synthetic (-)-pironetin and analogs

    doi: 10.1111/j.1747-0285.2009.00866.x

    Figure Lengend Snippet: (−)-Pironetin and 3 caused cancer-cell specific apoptosis and cell cycle arrest HeLa and IMR-90 cells (10,000 per well) were plated on the left and right halves, respectively, of a 384-well microplate. Wells were treated from right to left with 10 two-fold serial dilutions of drugs as shown. The two columns in the center of the plate show 14 replicates of vehicle treated control wells for each cell line. Starting concentrations for each agent are shown in parentheses. (A) Cell cycle arrest. Total Hoechst 33342 staining intensities from a minimum of 1,000 cells are presented as DNA content density distributions for each individual well on the microplate. Highlighted are the lowest concentrations where a shift in DNA content could be detected by visual inspection. (B) High-content analysis of apoptosis. HeLa, IMR-90, or A-549 cells treated with vehicle or ten two-fold dilutions of paclitaxel (□), vincristine (■), (−)-pironetin (●), 3 (○), or 4 (▲) were analyzed by high-content analysis for cell density, chromatin condensation, caspase cleavage, and p53 induction. Data are the averages of quadruplicate wells from a single experiment that has been repeated at least once with similar results. (HeLa, n=1).

    Article Snippet: Five transgenic zebrafish embryos ( Tg(Fli1:EGFP)y1 ) were then treated in 96-well plates in 200 μl E3 medium (5mM NaCl, 0.33mM CaCl2 , 0.17mM KCl, 0.33mM MgSO4 ) containing vehicle (DMSO, 0.5%), (−)- pironetin (5 μM to 0.5 μM), 3 (50μM) or SU11652 (Calbiochem) (40 μM) for 8 h or 24 h. The chorions were manually removed from treated embryos and single embryos were transferred to a 384-well plate containing 40μg/mL MS222 (tricaine methanesulfonate, Sigma) in E3 for imaging.

    Techniques: Staining, High Content Screening

    Design and synthesis of pironetin analogs A. Covalent tubulin modification by αLys352 conjugate addition to bound (−)-pironetin and a pironetin derivative designed to mimic interaction with αLys352 without covalent binding. B , Synthesis of pironetin derivatives 3 and 4 .

    Journal: Chemical biology & drug design

    Article Title: High-content analysis of cancer-cell specific apoptosis and inhibition of in vivo angiogenesis by synthetic (-)-pironetin and analogs

    doi: 10.1111/j.1747-0285.2009.00866.x

    Figure Lengend Snippet: Design and synthesis of pironetin analogs A. Covalent tubulin modification by αLys352 conjugate addition to bound (−)-pironetin and a pironetin derivative designed to mimic interaction with αLys352 without covalent binding. B , Synthesis of pironetin derivatives 3 and 4 .

    Article Snippet: Five transgenic zebrafish embryos ( Tg(Fli1:EGFP)y1 ) were then treated in 96-well plates in 200 μl E3 medium (5mM NaCl, 0.33mM CaCl2 , 0.17mM KCl, 0.33mM MgSO4 ) containing vehicle (DMSO, 0.5%), (−)- pironetin (5 μM to 0.5 μM), 3 (50μM) or SU11652 (Calbiochem) (40 μM) for 8 h or 24 h. The chorions were manually removed from treated embryos and single embryos were transferred to a 384-well plate containing 40μg/mL MS222 (tricaine methanesulfonate, Sigma) in E3 for imaging.

    Techniques: Modification, Binding Assay