Structured Review

Millipore 5 triphenyltetrazolium chloride
Curcumin pretreatment ameliorates MCAO/R-induced neurological impairment and early brain injury in rats. (A) Schematic representing the experimental protocol used for measuring the effects of curcumin pretreatment on brain injury. (B) Neurological Severity Scale scores in the different rat groups were evaluated at 24 h, and 3 and 7 days following reperfusion. (C) Rat brains were sliced and stained using <t>2,3,5-triphenyltetrazolium</t> chloride 24 h following MCAO/R and (D) the percentage of the relative infarct size was calculated. The data are presented as the mean ± SEM. n=6. ** P
5 Triphenyltetrazolium Chloride, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5 triphenyltetrazolium chloride/product/Millipore
Average 99 stars, based on 2 article reviews
Price from $9.99 to $1999.99
5 triphenyltetrazolium chloride - by Bioz Stars, 2022-10
99/100 stars

Images

1) Product Images from "Curcumin ameliorates ischemic stroke injury in rats by protecting the integrity of the blood-brain barrier"

Article Title: Curcumin ameliorates ischemic stroke injury in rats by protecting the integrity of the blood-brain barrier

Journal: Experimental and Therapeutic Medicine

doi: 10.3892/etm.2021.10215

Curcumin pretreatment ameliorates MCAO/R-induced neurological impairment and early brain injury in rats. (A) Schematic representing the experimental protocol used for measuring the effects of curcumin pretreatment on brain injury. (B) Neurological Severity Scale scores in the different rat groups were evaluated at 24 h, and 3 and 7 days following reperfusion. (C) Rat brains were sliced and stained using 2,3,5-triphenyltetrazolium chloride 24 h following MCAO/R and (D) the percentage of the relative infarct size was calculated. The data are presented as the mean ± SEM. n=6. ** P
Figure Legend Snippet: Curcumin pretreatment ameliorates MCAO/R-induced neurological impairment and early brain injury in rats. (A) Schematic representing the experimental protocol used for measuring the effects of curcumin pretreatment on brain injury. (B) Neurological Severity Scale scores in the different rat groups were evaluated at 24 h, and 3 and 7 days following reperfusion. (C) Rat brains were sliced and stained using 2,3,5-triphenyltetrazolium chloride 24 h following MCAO/R and (D) the percentage of the relative infarct size was calculated. The data are presented as the mean ± SEM. n=6. ** P

Techniques Used: Staining

2) Product Images from "Electroacupuncture Improves Neurobehavioral Function Through Targeting of SOX2-Mediated Axonal Regeneration by MicroRNA-132 After Ischemic Stroke"

Article Title: Electroacupuncture Improves Neurobehavioral Function Through Targeting of SOX2-Mediated Axonal Regeneration by MicroRNA-132 After Ischemic Stroke

Journal: Frontiers in Molecular Neuroscience

doi: 10.3389/fnmol.2018.00471

EA treatment alleviated brain injury and up-regulated miR-132 expression in ischemic penumbra after stroke. (A) 2, 3, and 5-triphenyltetrazolium chloride (TTC) staining was used to measure infarct volume in coronal brain sections from Sham, MCAO, and MCAO+EA-treated rats at 7 days after reperfusion. (B) Bar graph showed the infarct volume in coronal brain sections in each group. (C) Neurological score was used to assess recovery of neural function by EA treatment after stroke. (D) Bar graph showed the relative expression of miR-132 in the ischemic penumbra in each group. Data are expressed as means ± SEM ( n = 8 per group); ∗ p
Figure Legend Snippet: EA treatment alleviated brain injury and up-regulated miR-132 expression in ischemic penumbra after stroke. (A) 2, 3, and 5-triphenyltetrazolium chloride (TTC) staining was used to measure infarct volume in coronal brain sections from Sham, MCAO, and MCAO+EA-treated rats at 7 days after reperfusion. (B) Bar graph showed the infarct volume in coronal brain sections in each group. (C) Neurological score was used to assess recovery of neural function by EA treatment after stroke. (D) Bar graph showed the relative expression of miR-132 in the ischemic penumbra in each group. Data are expressed as means ± SEM ( n = 8 per group); ∗ p

Techniques Used: Expressing, Staining

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Millipore triphenyltetrazolium chloride
    Alda-1 decreased ALDH2 O -GlcNAc modification and improved myocardial ischemia/reperfusion injury exacerbated by hyperglycemia A . ALDH2 dehydrogenase activity in Con, HG, HG+H/R, HG+H/R+Alda-1 groups in vitro . ALDH2 activity was determined by measuring the conversion of NAD + to NADH. B . ALDH2 O -GlcNAcylation in Con, HG, HG+H/R, HG+H/R+Alda-1 groups in vitro . Cell lysates were immunoprecipitated with specific anti-ALDH2 antibody and then subjected to western blot using anti- O -GlcNAc (CDT110.6) antibody. C . ALDH2 O -GlcNAcylation in Sham, DM, DM+I/R, DM+I/R+Alda-1 groups in rats. D . LVEDP, LVESP, +LVdp/dt max and -LVdp/dt max in Sham, NS+I/R, DM+I/R, DM+I/R+Alda-1 groups in rats. LVEDP: left ventricular end diastolic pressure; LVESP: left ventricular end systolic pressure; +LVdp/dtmax, left ventricular maximal rate of pressure increase; -LVdp/dt max , left ventricular maximal rate of pressure decrease. E . Left: representative photographs of heart sections stained by 2, 3, <t>5-triphenyltetrazolium</t> (TTC) in Sham, NS+I/R, DM+I/R, DM+I/R+Alda-1 groups in rats; Right: myocardial infarct size expressed as percentage of the total left ventricular area. F . Left: representative photographs of in situ detection of apoptotic myocytes by TUNEL staining in Sham, NS+I/R, DM+I/R, DM+I/R+Alda-1 groups in rats; Right: percentage of TUNEL-positive nuclei in heart tissue sections. Scale bar, 50μm. Mannitol was used to keep the osmolarity consistent among different glucose concentrations in vitro . In A-C graphs, the mean value of Con or Sham was expressed as 1 or 100%. Con: normal glucose; HG: high glucose; H/R: hypoxia/reoxygenation; Alda-1: a specific ALDH2 activator; Sham, sham-operated; DM, diabetes; I/R, myocardial ischemia/reperfusion. Data are means ± SEM of 4 to 8 samples in each group. * P
    Triphenyltetrazolium Chloride, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triphenyltetrazolium chloride/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triphenyltetrazolium chloride - by Bioz Stars, 2022-10
    96/100 stars
      Buy from Supplier

    97
    Millipore ttc
    The effects of SA and LY on infarction volume and neurological deficits in rats following MCAO: ( A ) representative images of cerebral infarction after 2 h of MCAO and a 12 h reperfusion in rat brains by <t>2,3,5-triphenyltetrazolium</t> chloride <t>(TTC)</t> staining; ( B ) analysis of infarct volume, which is calculated as a percent relative to total cerebral volume ( n = 3 in each group. a p ≤ 0.01 vs. Sham; b p ≤ 0.05 vs. IR; c p ≤ 0.05 vs. SA); and ( C ) neurological deficits analysis ( n = 10 in each group. a p ≤ 0.01 vs. Sham; b p > 0.05 vs. IR; c p > 0.05 vs. SA). SA: salvianolic acids; MCAO: middle cerebral artery occlusion; LY: LY 294002; TTC: 2,3,5-triphenyltetrazolium chloride; IR: ischemia-reperfusion. Additionally, we investigated neurological deficits following MCAO, as shown in Figure 3 C. All rats in the sham group were scored zero. However, there was a significant deterioration in neurological functioning in the I/R group, compared to the sham group ( p ≤ 0.01). No improvement was noted in the scores in the SA groups compared to the I/R group after surgery.
    Ttc, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ttc/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ttc - by Bioz Stars, 2022-10
    97/100 stars
      Buy from Supplier

    Image Search Results


    Alda-1 decreased ALDH2 O -GlcNAc modification and improved myocardial ischemia/reperfusion injury exacerbated by hyperglycemia A . ALDH2 dehydrogenase activity in Con, HG, HG+H/R, HG+H/R+Alda-1 groups in vitro . ALDH2 activity was determined by measuring the conversion of NAD + to NADH. B . ALDH2 O -GlcNAcylation in Con, HG, HG+H/R, HG+H/R+Alda-1 groups in vitro . Cell lysates were immunoprecipitated with specific anti-ALDH2 antibody and then subjected to western blot using anti- O -GlcNAc (CDT110.6) antibody. C . ALDH2 O -GlcNAcylation in Sham, DM, DM+I/R, DM+I/R+Alda-1 groups in rats. D . LVEDP, LVESP, +LVdp/dt max and -LVdp/dt max in Sham, NS+I/R, DM+I/R, DM+I/R+Alda-1 groups in rats. LVEDP: left ventricular end diastolic pressure; LVESP: left ventricular end systolic pressure; +LVdp/dtmax, left ventricular maximal rate of pressure increase; -LVdp/dt max , left ventricular maximal rate of pressure decrease. E . Left: representative photographs of heart sections stained by 2, 3, 5-triphenyltetrazolium (TTC) in Sham, NS+I/R, DM+I/R, DM+I/R+Alda-1 groups in rats; Right: myocardial infarct size expressed as percentage of the total left ventricular area. F . Left: representative photographs of in situ detection of apoptotic myocytes by TUNEL staining in Sham, NS+I/R, DM+I/R, DM+I/R+Alda-1 groups in rats; Right: percentage of TUNEL-positive nuclei in heart tissue sections. Scale bar, 50μm. Mannitol was used to keep the osmolarity consistent among different glucose concentrations in vitro . In A-C graphs, the mean value of Con or Sham was expressed as 1 or 100%. Con: normal glucose; HG: high glucose; H/R: hypoxia/reoxygenation; Alda-1: a specific ALDH2 activator; Sham, sham-operated; DM, diabetes; I/R, myocardial ischemia/reperfusion. Data are means ± SEM of 4 to 8 samples in each group. * P

    Journal: Oncotarget

    Article Title: Inhibition of ALDH2 by O-GlcNAcylation contributes to the hyperglycemic exacerbation of myocardial ischemia/reperfusion injury

    doi: 10.18632/oncotarget.14297

    Figure Lengend Snippet: Alda-1 decreased ALDH2 O -GlcNAc modification and improved myocardial ischemia/reperfusion injury exacerbated by hyperglycemia A . ALDH2 dehydrogenase activity in Con, HG, HG+H/R, HG+H/R+Alda-1 groups in vitro . ALDH2 activity was determined by measuring the conversion of NAD + to NADH. B . ALDH2 O -GlcNAcylation in Con, HG, HG+H/R, HG+H/R+Alda-1 groups in vitro . Cell lysates were immunoprecipitated with specific anti-ALDH2 antibody and then subjected to western blot using anti- O -GlcNAc (CDT110.6) antibody. C . ALDH2 O -GlcNAcylation in Sham, DM, DM+I/R, DM+I/R+Alda-1 groups in rats. D . LVEDP, LVESP, +LVdp/dt max and -LVdp/dt max in Sham, NS+I/R, DM+I/R, DM+I/R+Alda-1 groups in rats. LVEDP: left ventricular end diastolic pressure; LVESP: left ventricular end systolic pressure; +LVdp/dtmax, left ventricular maximal rate of pressure increase; -LVdp/dt max , left ventricular maximal rate of pressure decrease. E . Left: representative photographs of heart sections stained by 2, 3, 5-triphenyltetrazolium (TTC) in Sham, NS+I/R, DM+I/R, DM+I/R+Alda-1 groups in rats; Right: myocardial infarct size expressed as percentage of the total left ventricular area. F . Left: representative photographs of in situ detection of apoptotic myocytes by TUNEL staining in Sham, NS+I/R, DM+I/R, DM+I/R+Alda-1 groups in rats; Right: percentage of TUNEL-positive nuclei in heart tissue sections. Scale bar, 50μm. Mannitol was used to keep the osmolarity consistent among different glucose concentrations in vitro . In A-C graphs, the mean value of Con or Sham was expressed as 1 or 100%. Con: normal glucose; HG: high glucose; H/R: hypoxia/reoxygenation; Alda-1: a specific ALDH2 activator; Sham, sham-operated; DM, diabetes; I/R, myocardial ischemia/reperfusion. Data are means ± SEM of 4 to 8 samples in each group. * P

    Article Snippet: Myocardial infarct area was determined by staining with 1% 2, 3, 5- triphenyltetrazolium chloride (TTC, 17779, Sigma-Aldrich, USA) and infarct size was expressed as percentage of left ventricular area.

    Techniques: Modification, Activity Assay, In Vitro, Immunoprecipitation, Western Blot, Staining, In Situ, TUNEL Assay

    Hyperglycemia exacerbated cardiac dysfunction, increased infarct size and apoptosis index after myocardial ischemia/reperfusion in rats A . LVEDP, LVESP, +LVdp/dt max and -LVdp/dt max . LVEDP, left ventricular end diastolic pressure; LVESP, left ventricular end systolic pressure; +LVdp/dt max , left ventricular maximal rate of pressure increase; -LVdp/dt max , left ventricular maximal rate of pressure decrease. B . Left: representative photographs of heart sections stained by 2, 3, 5-triphenyltetrazolium (TTC); Right: myocardial infarct size expressed as percentage of the total left ventricular area. C . Representative photographs of myocardial tissue with H E staining. Scale bar, 200μm. D . Left: representative photographs of in situ detection of apoptotic myocytes by TUNEL staining; Right: percentage of TUNEL-positive nuclei in heart tissue sections. Scale bar, 50μm. Sham, sham-operated; NS, normal saline; I/R, myocardial ischemia/reperfusion; AHG, acute hyperglycemia; DM, diabetes. Data are means ± SEM of 8 to 10 samples in each group. * P

    Journal: Oncotarget

    Article Title: Inhibition of ALDH2 by O-GlcNAcylation contributes to the hyperglycemic exacerbation of myocardial ischemia/reperfusion injury

    doi: 10.18632/oncotarget.14297

    Figure Lengend Snippet: Hyperglycemia exacerbated cardiac dysfunction, increased infarct size and apoptosis index after myocardial ischemia/reperfusion in rats A . LVEDP, LVESP, +LVdp/dt max and -LVdp/dt max . LVEDP, left ventricular end diastolic pressure; LVESP, left ventricular end systolic pressure; +LVdp/dt max , left ventricular maximal rate of pressure increase; -LVdp/dt max , left ventricular maximal rate of pressure decrease. B . Left: representative photographs of heart sections stained by 2, 3, 5-triphenyltetrazolium (TTC); Right: myocardial infarct size expressed as percentage of the total left ventricular area. C . Representative photographs of myocardial tissue with H E staining. Scale bar, 200μm. D . Left: representative photographs of in situ detection of apoptotic myocytes by TUNEL staining; Right: percentage of TUNEL-positive nuclei in heart tissue sections. Scale bar, 50μm. Sham, sham-operated; NS, normal saline; I/R, myocardial ischemia/reperfusion; AHG, acute hyperglycemia; DM, diabetes. Data are means ± SEM of 8 to 10 samples in each group. * P

    Article Snippet: Myocardial infarct area was determined by staining with 1% 2, 3, 5- triphenyltetrazolium chloride (TTC, 17779, Sigma-Aldrich, USA) and infarct size was expressed as percentage of left ventricular area.

    Techniques: Staining, In Situ, TUNEL Assay

    The effects of SA and LY on infarction volume and neurological deficits in rats following MCAO: ( A ) representative images of cerebral infarction after 2 h of MCAO and a 12 h reperfusion in rat brains by 2,3,5-triphenyltetrazolium chloride (TTC) staining; ( B ) analysis of infarct volume, which is calculated as a percent relative to total cerebral volume ( n = 3 in each group. a p ≤ 0.01 vs. Sham; b p ≤ 0.05 vs. IR; c p ≤ 0.05 vs. SA); and ( C ) neurological deficits analysis ( n = 10 in each group. a p ≤ 0.01 vs. Sham; b p > 0.05 vs. IR; c p > 0.05 vs. SA). SA: salvianolic acids; MCAO: middle cerebral artery occlusion; LY: LY 294002; TTC: 2,3,5-triphenyltetrazolium chloride; IR: ischemia-reperfusion. Additionally, we investigated neurological deficits following MCAO, as shown in Figure 3 C. All rats in the sham group were scored zero. However, there was a significant deterioration in neurological functioning in the I/R group, compared to the sham group ( p ≤ 0.01). No improvement was noted in the scores in the SA groups compared to the I/R group after surgery.

    Journal: International Journal of Molecular Sciences

    Article Title: Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury

    doi: 10.3390/ijms17071190

    Figure Lengend Snippet: The effects of SA and LY on infarction volume and neurological deficits in rats following MCAO: ( A ) representative images of cerebral infarction after 2 h of MCAO and a 12 h reperfusion in rat brains by 2,3,5-triphenyltetrazolium chloride (TTC) staining; ( B ) analysis of infarct volume, which is calculated as a percent relative to total cerebral volume ( n = 3 in each group. a p ≤ 0.01 vs. Sham; b p ≤ 0.05 vs. IR; c p ≤ 0.05 vs. SA); and ( C ) neurological deficits analysis ( n = 10 in each group. a p ≤ 0.01 vs. Sham; b p > 0.05 vs. IR; c p > 0.05 vs. SA). SA: salvianolic acids; MCAO: middle cerebral artery occlusion; LY: LY 294002; TTC: 2,3,5-triphenyltetrazolium chloride; IR: ischemia-reperfusion. Additionally, we investigated neurological deficits following MCAO, as shown in Figure 3 C. All rats in the sham group were scored zero. However, there was a significant deterioration in neurological functioning in the I/R group, compared to the sham group ( p ≤ 0.01). No improvement was noted in the scores in the SA groups compared to the I/R group after surgery.

    Article Snippet: TTC Staining 2,3,5-triphenyltetrazolium chloride (TTC, Sigma, St. Louis, MO, USA) staining was used to visualize the ischemic infarction.

    Techniques: Staining

    Effects of granulocyte-colony stimulating factor (G-CSF) on delayed tissue plasminogen activator (tPA)-induced hemorrhage and cerebral infarction in rats subjected to thromboembolic stroke. ( A ) Photographs are representative coronal brain sections obtained 3 days after stroke, showing that tPA treatment produced remarkable hemispheric hemorrhage, whereas the combined treatment with G-CSF did not result in bleeding; ( B ) Infarct volume was reduced, and hemorrhage was not observed in rats treated with G-CSF 7 days after stroke; ( C ) Representative coronal brain sections stained with 2,3,5-triphenyltetrazolium chloride (TTC) 3 days after stroke showing the infarct area (white) and intact areas (red); ( D ) Quantitative analysis of spectrophotometric assay findings showing the incidence of hemorrhage in rats subjected to delayed tPA treatment, which was reduced by 47% by G-CSF treatment; ( E ) Quantitative analysis of infarct volume in vehicle-, tPA-, and G-CSF + tPA-treated groups. G-CSF reduced infarct volume by 33% in stroked rats subjected to delayed tPA treatment; * p

    Journal: International Journal of Molecular Sciences

    Article Title: Extension of Tissue Plasminogen Activator Treatment Window by Granulocyte-Colony Stimulating Factor in a Thromboembolic Rat Model of Stroke

    doi: 10.3390/ijms19061635

    Figure Lengend Snippet: Effects of granulocyte-colony stimulating factor (G-CSF) on delayed tissue plasminogen activator (tPA)-induced hemorrhage and cerebral infarction in rats subjected to thromboembolic stroke. ( A ) Photographs are representative coronal brain sections obtained 3 days after stroke, showing that tPA treatment produced remarkable hemispheric hemorrhage, whereas the combined treatment with G-CSF did not result in bleeding; ( B ) Infarct volume was reduced, and hemorrhage was not observed in rats treated with G-CSF 7 days after stroke; ( C ) Representative coronal brain sections stained with 2,3,5-triphenyltetrazolium chloride (TTC) 3 days after stroke showing the infarct area (white) and intact areas (red); ( D ) Quantitative analysis of spectrophotometric assay findings showing the incidence of hemorrhage in rats subjected to delayed tPA treatment, which was reduced by 47% by G-CSF treatment; ( E ) Quantitative analysis of infarct volume in vehicle-, tPA-, and G-CSF + tPA-treated groups. G-CSF reduced infarct volume by 33% in stroked rats subjected to delayed tPA treatment; * p

    Article Snippet: To measure infarct volume, coronal sections of the rats’ brains were stained with 2% 2,3,5-triphenyltetrazolium chloride (TTC) (Sigma, St. Louis, MO, USA), fixed with 4% paraformaldehyde, scanned, and analyzed to determine the ratio of infarct area to the whole brain, using ImageJ software (ImageJ2, Bethesda, MD, USA).

    Techniques: Produced, Staining, Spectrophotometric Assay

    Effects of electro-acupuncture (EA) on cerebral infarction after stroke. (A) Cerebral tissues from each group were coronally sectioned into 2-mm-thick slices and then processed for 2,3,5-triphenyltetrazolium chloride (TTC) staining. Normal areas of the brain were stained deep red, indicating intact mitochondrial function, whereas the infarct areas remained unstained. Representative images were captured using a high-resolution digital camera. (B) Infarct volume was quantified using the Motic Med 6.0 system, and is presented as a percentage of the total brain volume. Data shown are the means ± SEM from 5 individual rats in each group. ## P

    Journal: International Journal of Molecular Medicine

    Article Title: Electro-acupuncture exerts beneficial effects against cerebral ischemia and promotes the proliferation of neural progenitor cells in the cortical peri-infarct area through the Wnt/β-catenin signaling pathway

    doi: 10.3892/ijmm.2015.2334

    Figure Lengend Snippet: Effects of electro-acupuncture (EA) on cerebral infarction after stroke. (A) Cerebral tissues from each group were coronally sectioned into 2-mm-thick slices and then processed for 2,3,5-triphenyltetrazolium chloride (TTC) staining. Normal areas of the brain were stained deep red, indicating intact mitochondrial function, whereas the infarct areas remained unstained. Representative images were captured using a high-resolution digital camera. (B) Infarct volume was quantified using the Motic Med 6.0 system, and is presented as a percentage of the total brain volume. Data shown are the means ± SEM from 5 individual rats in each group. ## P

    Article Snippet: The fresh slices were incubated in 2% (w/v) 2,3,5-triphenyltetrazolium chloride (TTC; Sigma-Aldrich) solution in phosphate-buffered saline (PBS; HyClone, Beijing, China) for 20 min, at 37°C in the dark.

    Techniques: Staining