2 diphenyl 1 picrylhydrazyl dpph  (Millipore)


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    Name:
    2 2 Diphenyl 1 picrylhydrazyl
    Description:
    2 2 diphenyl 1 picrylhydrazyl is a free radical which shows hydrogen acceptor ability towards antioxidants Hence it is commonly used in DPPH assay for measuring the antioxidant activity of different natural samples such as wine fruits herbal tea etc
    Catalog Number:
    d9132
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    Structured Review

    Millipore 2 diphenyl 1 picrylhydrazyl dpph
    2 2 Diphenyl 1 picrylhydrazyl
    2 2 diphenyl 1 picrylhydrazyl is a free radical which shows hydrogen acceptor ability towards antioxidants Hence it is commonly used in DPPH assay for measuring the antioxidant activity of different natural samples such as wine fruits herbal tea etc
    https://www.bioz.com/result/2 diphenyl 1 picrylhydrazyl dpph/product/Millipore
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    2 diphenyl 1 picrylhydrazyl dpph - by Bioz Stars, 2021-01
    99/100 stars

    Images

    1) Product Images from "Characteristics and kinetics of catalpol degradation and the effect of its degradation products on free radical scavenging"

    Article Title: Characteristics and kinetics of catalpol degradation and the effect of its degradation products on free radical scavenging

    Journal: Pharmacognosy Magazine

    doi: 10.4103/0973-1296.127360

    Catalpol degradation (30 mM) at 90°C and pH 4.0 for 4 h in the presence of amino acids (30 mM). (a) degradation rate; (b) absorbance at 420 nm of the 80-fold dilution reaction solution; (c) 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging ability of the 40-fold dilution reaction solution (at full page width)
    Figure Legend Snippet: Catalpol degradation (30 mM) at 90°C and pH 4.0 for 4 h in the presence of amino acids (30 mM). (a) degradation rate; (b) absorbance at 420 nm of the 80-fold dilution reaction solution; (c) 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging ability of the 40-fold dilution reaction solution (at full page width)

    Techniques Used: Polyacrylamide Gel Electrophoresis

    Degradation of catalpol (30 mM) at 90°C and different pH values for 4 h in the presence of different concentrations of glycine(△, 3 mM; □ 30 mM, △, 300 mM) (a) catalpol degradation rate; (b) absorbance at 420 nm of the 100-fold dilution reaction solution; (c) 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging ability of the 50-fold dilution of the reaction solution (at column width)
    Figure Legend Snippet: Degradation of catalpol (30 mM) at 90°C and different pH values for 4 h in the presence of different concentrations of glycine(△, 3 mM; □ 30 mM, △, 300 mM) (a) catalpol degradation rate; (b) absorbance at 420 nm of the 100-fold dilution reaction solution; (c) 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging ability of the 50-fold dilution of the reaction solution (at column width)

    Techniques Used:

    2) Product Images from "Antioxidative activity and protein profile of skim milk of Gaddi goats and hill cattle of North West Himalayan region"

    Article Title: Antioxidative activity and protein profile of skim milk of Gaddi goats and hill cattle of North West Himalayan region

    Journal: Veterinary World

    doi: 10.14202/vetworld.2019.1535-1539

    Antioxidant activity as elucidated by qualitative 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) test using F 254 thin-layer chromatography silica coated-aluminum plates. (a) Color developed immediately after DPPH spray; (b) yellow spot appearing after 30 min., (c) yellow spot after 18 h. Lane 1 – ascorbic acid (+ve control); lane 2 – methanol (−ve control); lanes 3 and 4 – skim milk of indigenous hill cattle; lanes 5 and 6 – skim milk of Jersey crossbred cattle; lanes 7 and 8 – skim milk of Gaddi goat; lanes 9 and 10 – skim milk of non-Gaddi goats. Each spot was developed from 10 µl of samples.
    Figure Legend Snippet: Antioxidant activity as elucidated by qualitative 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) test using F 254 thin-layer chromatography silica coated-aluminum plates. (a) Color developed immediately after DPPH spray; (b) yellow spot appearing after 30 min., (c) yellow spot after 18 h. Lane 1 – ascorbic acid (+ve control); lane 2 – methanol (−ve control); lanes 3 and 4 – skim milk of indigenous hill cattle; lanes 5 and 6 – skim milk of Jersey crossbred cattle; lanes 7 and 8 – skim milk of Gaddi goat; lanes 9 and 10 – skim milk of non-Gaddi goats. Each spot was developed from 10 µl of samples.

    Techniques Used: Antioxidant Activity Assay, Thin Layer Chromatography

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    Millipore 2 2 diphenyl 1 picrylhydrazyl
    Determination of DPPH radical scavenging activity and intracellular ROS production. (A) DPPH radical scavenging activity of EMfC was assayed in a mixture containing 0.1 mM DPPH and varying concentrations of EMfC (0-1,000 µg/ml). For each dose, two to three wells were evaluated, and the optical density was measured in duplicate for each sample. (B) Determination of intracellular ROS production. Following DCFH-DA treatment of EMfC+LPS-treated RAW264.7 cells, green fluorescence was examined in the subset groups of cells using a fluorescence microscope. Magnification, ×200. DPPH, <t>2,2-diphenyl-1-picrylhydrazyl;</t> EMfC, ethanol extracts of mulberry leaves fermented with C. militaris ; LPS, lipopolysaccharide; ROS, reactive oxygen species; Lo, low; Mi, medium; Hi, high.
    2 2 Diphenyl 1 Picrylhydrazyl, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 2 diphenyl 1 picrylhydrazyl/product/Millipore
    Average 99 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    2 2 diphenyl 1 picrylhydrazyl - by Bioz Stars, 2021-01
    99/100 stars
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    Determination of DPPH radical scavenging activity and intracellular ROS production. (A) DPPH radical scavenging activity of EMfC was assayed in a mixture containing 0.1 mM DPPH and varying concentrations of EMfC (0-1,000 µg/ml). For each dose, two to three wells were evaluated, and the optical density was measured in duplicate for each sample. (B) Determination of intracellular ROS production. Following DCFH-DA treatment of EMfC+LPS-treated RAW264.7 cells, green fluorescence was examined in the subset groups of cells using a fluorescence microscope. Magnification, ×200. DPPH, 2,2-diphenyl-1-picrylhydrazyl; EMfC, ethanol extracts of mulberry leaves fermented with C. militaris ; LPS, lipopolysaccharide; ROS, reactive oxygen species; Lo, low; Mi, medium; Hi, high.

    Journal: Molecular Medicine Reports

    Article Title: Protective role of fermented mulberry leave extract in LPS-induced inflammation and autophagy of RAW264.7 macrophage cells

    doi: 10.3892/mmr.2020.11563

    Figure Lengend Snippet: Determination of DPPH radical scavenging activity and intracellular ROS production. (A) DPPH radical scavenging activity of EMfC was assayed in a mixture containing 0.1 mM DPPH and varying concentrations of EMfC (0-1,000 µg/ml). For each dose, two to three wells were evaluated, and the optical density was measured in duplicate for each sample. (B) Determination of intracellular ROS production. Following DCFH-DA treatment of EMfC+LPS-treated RAW264.7 cells, green fluorescence was examined in the subset groups of cells using a fluorescence microscope. Magnification, ×200. DPPH, 2,2-diphenyl-1-picrylhydrazyl; EMfC, ethanol extracts of mulberry leaves fermented with C. militaris ; LPS, lipopolysaccharide; ROS, reactive oxygen species; Lo, low; Mi, medium; Hi, high.

    Article Snippet: Scavenging activity of free radical The scavenging capability of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical was measured at eight different concentrations of EMfC (0-1,000 µg/ml), according to the method described in a previous study ( ).

    Techniques: Activity Assay, Fluorescence, Microscopy

    Antioxidant activities of soluble extracts of decorticated sorghum varieties. ( A ) 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity IC 50 values; ( B ) 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity IC 50 values. Different lowercase letters denote significant difference ( p

    Journal: Molecules

    Article Title: Flavonoids in Decorticated Sorghum Grains Exert Antioxidant, Antidiabetic and Antiobesity Activities

    doi: 10.3390/molecules25122854

    Figure Lengend Snippet: Antioxidant activities of soluble extracts of decorticated sorghum varieties. ( A ) 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity IC 50 values; ( B ) 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity IC 50 values. Different lowercase letters denote significant difference ( p

    Article Snippet: Analytical grade reagents including potassium phosphate monobasic, potassium phosphate dibasic, sodium dihydrogen phosphate, sodium phosphate dibasic, sodium chloride, sodium carbonate, soluble starch, sodium hydroxide, 3,5-dinitrosalicyclic acid, sodium citrate, 4-nitrophenyl α-d-glucopyranoside, 2,2′-diphenyl-1-picrylhydrazyl (DPPH), Folin–Ciocalteu reagent, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), ethanol, hexane, methanol, hydrochloric acid, potassium persulfate and sodium carbonate were purchased.

    Techniques: Activity Assay

    A. saligna and L. inermis antioxidant effects using 2,2-diphenyl-1-picrylhydrazyl (DPPH), β-Carotene bleaching and ferric reducing antioxidant power (FRAP) assays (expressed as IC 50 in µg/mL). Identified polyphenol (rutoside, hyperoside, quercetin 3-glucuronide, gallic acid, isoquercetin, p -coumaric acid, quercitrin, apigetrin and apigenin 5-glucoside) antioxidant effects were also added. Values are expressed as mean ± standard deviation. TEAC: Trolox equivalent antioxidant capacity. Different letters among group of columns indicate significant differences ( p ≤ 0.05).

    Journal: Plants

    Article Title: Antioxidant and Biological Activities of Acacia saligna and Lawsonia inermis Natural Populations

    doi: 10.3390/plants9070908

    Figure Lengend Snippet: A. saligna and L. inermis antioxidant effects using 2,2-diphenyl-1-picrylhydrazyl (DPPH), β-Carotene bleaching and ferric reducing antioxidant power (FRAP) assays (expressed as IC 50 in µg/mL). Identified polyphenol (rutoside, hyperoside, quercetin 3-glucuronide, gallic acid, isoquercetin, p -coumaric acid, quercitrin, apigetrin and apigenin 5-glucoside) antioxidant effects were also added. Values are expressed as mean ± standard deviation. TEAC: Trolox equivalent antioxidant capacity. Different letters among group of columns indicate significant differences ( p ≤ 0.05).

    Article Snippet: Antioxidant Activity The antioxidant activities of leaf extracts of A. saligna and L. inermis were determined using ferric reducing antioxidant power (FRAP), β-carotene bleaching and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays [ , , , , ].

    Techniques: Standard Deviation