1x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 1x nebuffer 2 1
    1x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1x nebuffer 2 1 buffer  (New England Biolabs)


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    New England Biolabs 1x nebuffer 2 1 buffer
    1x Nebuffer 2 1 Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    86/100 stars

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    1x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 1x nebuffer 2 1
    1x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    1x nebuffer 2 1 - by Bioz Stars, 2023-12
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    1x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 1x nebuffer 2 1
    (a) The concentration of Cas enzyme and crRNA, together referred to as ribonucleoprotein (RNP) was tuned for most sensitive detection of AAV genomic titer. 4 RNP concentrations were tested against AAV genome titers ranging from 1e8 to 5e9 vg/mL. 10 nM RNP was selected was the most robust assay condition. Next, the standard NEBuffer 2.1, typically containing 10 mM Mg 2+ was custom prepared to test the effects of modifying magnesium concentration. Results after (b) 30 minutes and (c) 60 minutes of reaction show that increasing the Magnesium above 10 mM provide additional sensitivity represented by signal fold change over the NTC, where error bars represent standard deviation (n = 3). Statistical analysis was performed using a two tailed student’s t-test, where ns = not significant with p > 0.05, and asterisks (* = p < 0.005, ** = p < 0.0005) represent significant differences. (d) Buffer composition effect on CRAAVE assay sensitivity was further assessed by modifying both NaCl and MgCl 2 concentrations. Significantly better sensitivity was achieved when total ionic strength was lowered, but magnesium concentration was kept at 20 mM.
    1x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x nebuffer 2 1/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x nebuffer 2 1 - by Bioz Stars, 2023-12
    86/100 stars

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    1) Product Images from "Rapid Adeno-Associated Virus Genome Quantification with Amplification-Free CRISPR-Cas12a"

    Article Title: Rapid Adeno-Associated Virus Genome Quantification with Amplification-Free CRISPR-Cas12a

    Journal: bioRxiv

    doi: 10.1101/2023.11.14.567134

    (a) The concentration of Cas enzyme and crRNA, together referred to as ribonucleoprotein (RNP) was tuned for most sensitive detection of AAV genomic titer. 4 RNP concentrations were tested against AAV genome titers ranging from 1e8 to 5e9 vg/mL. 10 nM RNP was selected was the most robust assay condition. Next, the standard NEBuffer 2.1, typically containing 10 mM Mg 2+ was custom prepared to test the effects of modifying magnesium concentration. Results after (b) 30 minutes and (c) 60 minutes of reaction show that increasing the Magnesium above 10 mM provide additional sensitivity represented by signal fold change over the NTC, where error bars represent standard deviation (n = 3). Statistical analysis was performed using a two tailed student’s t-test, where ns = not significant with p > 0.05, and asterisks (* = p < 0.005, ** = p < 0.0005) represent significant differences. (d) Buffer composition effect on CRAAVE assay sensitivity was further assessed by modifying both NaCl and MgCl 2 concentrations. Significantly better sensitivity was achieved when total ionic strength was lowered, but magnesium concentration was kept at 20 mM.
    Figure Legend Snippet: (a) The concentration of Cas enzyme and crRNA, together referred to as ribonucleoprotein (RNP) was tuned for most sensitive detection of AAV genomic titer. 4 RNP concentrations were tested against AAV genome titers ranging from 1e8 to 5e9 vg/mL. 10 nM RNP was selected was the most robust assay condition. Next, the standard NEBuffer 2.1, typically containing 10 mM Mg 2+ was custom prepared to test the effects of modifying magnesium concentration. Results after (b) 30 minutes and (c) 60 minutes of reaction show that increasing the Magnesium above 10 mM provide additional sensitivity represented by signal fold change over the NTC, where error bars represent standard deviation (n = 3). Statistical analysis was performed using a two tailed student’s t-test, where ns = not significant with p > 0.05, and asterisks (* = p < 0.005, ** = p < 0.0005) represent significant differences. (d) Buffer composition effect on CRAAVE assay sensitivity was further assessed by modifying both NaCl and MgCl 2 concentrations. Significantly better sensitivity was achieved when total ionic strength was lowered, but magnesium concentration was kept at 20 mM.

    Techniques Used: Concentration Assay, Standard Deviation, Two Tailed Test

    1x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 1x nebuffer 2 1
    (a) The concentration of Cas enzyme and crRNA, together referred to as ribonucleoprotein (RNP) was tuned for most sensitive detection of AAV genomic titer. 4 RNP concentrations were tested against AAV genome titers ranging from 1e8 to 5e9 vg/mL. 10 nM RNP was selected was the most robust assay condition. Next, the standard NEBuffer 2.1, typically containing 10 mM Mg 2+ was custom prepared to test the effects of modifying magnesium concentration. Results after (b) 30 minutes and (c) 60 minutes of reaction show that increasing the Magnesium above 10 mM provide additional sensitivity represented by signal fold change over the NTC, where error bars represent standard deviation (n = 3). Statistical analysis was performed using a two tailed student’s t-test, where ns = not significant with p > 0.05, and asterisks (* = p < 0.005, ** = p < 0.0005) represent significant differences. (d) Buffer composition effect on CRAAVE assay sensitivity was further assessed by modifying both NaCl and MgCl 2 concentrations. Significantly better sensitivity was achieved when total ionic strength was lowered, but magnesium concentration was kept at 20 mM.
    1x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x nebuffer 2 1/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x nebuffer 2 1 - by Bioz Stars, 2023-12
    86/100 stars

    Images

    1) Product Images from "Rapid Adeno-Associated Virus Genome Quantification with Amplification-Free CRISPR-Cas12a"

    Article Title: Rapid Adeno-Associated Virus Genome Quantification with Amplification-Free CRISPR-Cas12a

    Journal: bioRxiv

    doi: 10.1101/2023.11.14.567134

    (a) The concentration of Cas enzyme and crRNA, together referred to as ribonucleoprotein (RNP) was tuned for most sensitive detection of AAV genomic titer. 4 RNP concentrations were tested against AAV genome titers ranging from 1e8 to 5e9 vg/mL. 10 nM RNP was selected was the most robust assay condition. Next, the standard NEBuffer 2.1, typically containing 10 mM Mg 2+ was custom prepared to test the effects of modifying magnesium concentration. Results after (b) 30 minutes and (c) 60 minutes of reaction show that increasing the Magnesium above 10 mM provide additional sensitivity represented by signal fold change over the NTC, where error bars represent standard deviation (n = 3). Statistical analysis was performed using a two tailed student’s t-test, where ns = not significant with p > 0.05, and asterisks (* = p < 0.005, ** = p < 0.0005) represent significant differences. (d) Buffer composition effect on CRAAVE assay sensitivity was further assessed by modifying both NaCl and MgCl 2 concentrations. Significantly better sensitivity was achieved when total ionic strength was lowered, but magnesium concentration was kept at 20 mM.
    Figure Legend Snippet: (a) The concentration of Cas enzyme and crRNA, together referred to as ribonucleoprotein (RNP) was tuned for most sensitive detection of AAV genomic titer. 4 RNP concentrations were tested against AAV genome titers ranging from 1e8 to 5e9 vg/mL. 10 nM RNP was selected was the most robust assay condition. Next, the standard NEBuffer 2.1, typically containing 10 mM Mg 2+ was custom prepared to test the effects of modifying magnesium concentration. Results after (b) 30 minutes and (c) 60 minutes of reaction show that increasing the Magnesium above 10 mM provide additional sensitivity represented by signal fold change over the NTC, where error bars represent standard deviation (n = 3). Statistical analysis was performed using a two tailed student’s t-test, where ns = not significant with p > 0.05, and asterisks (* = p < 0.005, ** = p < 0.0005) represent significant differences. (d) Buffer composition effect on CRAAVE assay sensitivity was further assessed by modifying both NaCl and MgCl 2 concentrations. Significantly better sensitivity was achieved when total ionic strength was lowered, but magnesium concentration was kept at 20 mM.

    Techniques Used: Concentration Assay, Standard Deviation, Two Tailed Test

    1x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 1x nebuffer 2 1
    1x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 1x nebuffer 2 1
    1x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x nebuffer 2 1/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
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    1x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 1x nebuffer 2 1
    1x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    1x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 1x nebuffer 2 1
    1x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 1x nebuffer 2 1
    1x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 1x nebuffer 2 1
    1x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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