1x neaa  (Thermo Fisher)


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    Name:
    MEM Non Essential Amino Acids Solution 100X
    Description:
    Gibco MEM Non Essential Amino Acids Solution is used as a growth supplement for cell culture medium to increase cell growth and viability Gibco MEM Non Essential Amino Acids Solution is formulated to contain 100X the non essential amino acids found in the standard Minimum Essential Medium MEM The complete formulation is available Product UseFor in vitro diagnostic use Dual site cGMP Manufacturing and Quality SystemGibco MEM Non Essential Amino Acids Solution is manufactured at a cGMP compliant facility located in Paisley Scotland UK The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard For supply chain continuity we offer an identical Gibco MEM Non Essential Amino Acids Solution product made in our Grand Island facility 11140 050 This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards
    Catalog Number:
    11140035
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|Mammalian Cell Culture
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    Structured Review

    Thermo Fisher 1x neaa
    Differentiation of iPSCs into iPS-NSCs. iPSCs were expanded to 80% confluency, and then, the iPSC media were replaced by neuronal induction media (Neurobasal-A, 1X B27-A, 1X N2, <t>1X</t> NEAA, 1X Glutamax, and 5 mg/mL streptomycin and 5 UI/mL penicillin). The cells were kept in culture until they detached and formed neurospheres. The media containing detached cells were centrifuged, and the pellet was dissociated in Accutase. Cells were then re-plated in neural stem cell media (Neurobasal-A, 1X B27-A, 1X N2, 1X NEAA, 1X Glutamax, 20 ng/mL EGF, 10 ng/mL bFGF, and 5 mg/mL streptomycin and 5 UI/mL penicillin). Cells were passaged three times and then characterized using ICC.
    Gibco MEM Non Essential Amino Acids Solution is used as a growth supplement for cell culture medium to increase cell growth and viability Gibco MEM Non Essential Amino Acids Solution is formulated to contain 100X the non essential amino acids found in the standard Minimum Essential Medium MEM The complete formulation is available Product UseFor in vitro diagnostic use Dual site cGMP Manufacturing and Quality SystemGibco MEM Non Essential Amino Acids Solution is manufactured at a cGMP compliant facility located in Paisley Scotland UK The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard For supply chain continuity we offer an identical Gibco MEM Non Essential Amino Acids Solution product made in our Grand Island facility 11140 050 This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards
    https://www.bioz.com/result/1x neaa/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x neaa - by Bioz Stars, 2021-03
    97/100 stars

    Images

    1) Product Images from "Induced Pluripotent Stem Cell-Derived Neural Stem Cell Transplantations Reduced Behavioral Deficits and Ameliorated Neuropathological Changes in YAC128 Mouse Model of Huntington's Disease"

    Article Title: Induced Pluripotent Stem Cell-Derived Neural Stem Cell Transplantations Reduced Behavioral Deficits and Ameliorated Neuropathological Changes in YAC128 Mouse Model of Huntington's Disease

    Journal: Frontiers in Neuroscience

    doi: 10.3389/fnins.2017.00628

    Differentiation of iPSCs into iPS-NSCs. iPSCs were expanded to 80% confluency, and then, the iPSC media were replaced by neuronal induction media (Neurobasal-A, 1X B27-A, 1X N2, 1X NEAA, 1X Glutamax, and 5 mg/mL streptomycin and 5 UI/mL penicillin). The cells were kept in culture until they detached and formed neurospheres. The media containing detached cells were centrifuged, and the pellet was dissociated in Accutase. Cells were then re-plated in neural stem cell media (Neurobasal-A, 1X B27-A, 1X N2, 1X NEAA, 1X Glutamax, 20 ng/mL EGF, 10 ng/mL bFGF, and 5 mg/mL streptomycin and 5 UI/mL penicillin). Cells were passaged three times and then characterized using ICC.
    Figure Legend Snippet: Differentiation of iPSCs into iPS-NSCs. iPSCs were expanded to 80% confluency, and then, the iPSC media were replaced by neuronal induction media (Neurobasal-A, 1X B27-A, 1X N2, 1X NEAA, 1X Glutamax, and 5 mg/mL streptomycin and 5 UI/mL penicillin). The cells were kept in culture until they detached and formed neurospheres. The media containing detached cells were centrifuged, and the pellet was dissociated in Accutase. Cells were then re-plated in neural stem cell media (Neurobasal-A, 1X B27-A, 1X N2, 1X NEAA, 1X Glutamax, 20 ng/mL EGF, 10 ng/mL bFGF, and 5 mg/mL streptomycin and 5 UI/mL penicillin). Cells were passaged three times and then characterized using ICC.

    Techniques Used: Immunocytochemistry

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    Cell Differentiation:

    Article Title: A Testis-Derived Hydrogel as an Efficient Feeder-Free Culture Platform to Promote Mouse Spermatogonial Stem Cell Proliferation and Differentiation
    Article Snippet: Then, the single-cell suspensions (approximately 1 × 105 cells in 10 μl of PBS) were injected into the seminiferous tubules of recipient mice through the efferent ducts using a micropipette (40–50 μm diameter tips). .. Spermatogonial Stem Cell Differentiation SSCs were plated on laminin, Matrigel, and DTM hydrogel scaffolds with SSC differentiation medium composed of DMEM, 10% FBS, minimal essential medium (MEM) non-essential amino acids (Thermo Fisher Scientific, Waltham, MA, United States), 10 μM testosterone (Cayman, Michigan, United States), 100 ng/ml follicle stimulating hormone (FSH) (ProSpec, Rocky Hill, United States), and 100 ng/ml retinoic acid (RA) (Sigma, Poole, Dorset, United Kingdom) at 34°C under 5% CO2. ..

    Cell Culture:

    Article Title: The Distinct Role of Tcfs and Lef1 in the Self-Renewal or Differentiation of Mouse Embryonic Stem Cells
    Article Snippet: .. Culture and differentiation of mouse ES cells A6P10 mES cells (a gift from Dr. Chyuan-Sheng Lin, Columbia University, USA) and 46C mES cells (ES cell line in which EGFP was replaced into the open reading frame of Sox1 gene, provided by Dr. Qilong Ying, University of Southern California, USA) were cultured in ES medium (DMEM (Gibco) with 15% FBS, 2 mM GlutaMAX (Gibco), MEM nonessential amino acids, β -mercaptoethanol (Gibco), tylosin, 1% Pen/Strep (Gibco)) supplemented with LIF (Chemicon) on 0.2% gelatin-coated dishes. .. To induce neuronal differentiation, 46C cells were cultured in N2B27 medium (DMEM/F12 (Gibco), Neurobasal medium (Gibco), N2 supplement (Invitrogen), B27 supplement (Invitrogen), 1 mM GlutaMAX (Gibco), 0.1 μ M β -mercaptoethanol (Gibco), 1% Pen/Strep (Gibco)) on 0.2% gelatin-coated tissue culture dish (Falcon).

    Article Title: Active macropinocytosis induction by stimulation of epidermal growth factor receptor and oncogenic Ras expression potentiates cellular uptake efficacy of exosomes
    Article Snippet: The human epidermoid carcinoma-derived A431 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). .. The cells were cultured in minimum essential medium α (α-MEM; Gibco, Life Technologies Corporation, Grand Island, NY, USA) containing 10% heat-inactivated foetal bovine serum (FBS; Gibco, Life Technologies Corporation) (HeLa cells), minimum essential medium (MEM) (Gibco, Life Technologies Corporation) containing 10% heat-inactivated FBS (Gibco, Life Technologies Corporation) (A431 cells), Eagle’s minimum essential medium (EMEM) (Wako, Osaka, Japan) containing 10% FBS (HyClone Laboratories, South Logan, UT, USA), 0.1 mM MEM non-essential amino acids (Gibco), penicillin-streptomycin (50 units/ml and 50 μg/ml) (Sigma-Aldrich, St. Louis, MO, USA) (MIA PaCa-2 cells), and RPMI1640 (Gibco, Life Technologies Corporation) containing 10% FBS (HyClone), and penicillin-streptomycin (50 units/ml and 50 μg/ml) (Sigma-Aldrich) (BxPC-3 cells). ..

    Article Title: Colorectal Mucus Binds DC-SIGN and Inhibits HIV-1 Trans-Infection of CD4+ T-Lymphocytes
    Article Snippet: For development, Lumi-phos plus (Lumigen Inc.) was used according to the manufacturer's protocol and as a standard curve a serial dilution of Escherichia coli -expressed recombinant HIV-1-p24 (Aalto Bio Reagents Ltd.) was analyzed. .. Cells TZM-bl cells were cultured in DMEM (Invitrogen) containing 10% FCS, MEM non-essential amino acids (0.1mM, Invitrogen), penicillin and streptavidin (pen/strep), both 100U/ml. .. Raji DC-SIGN cells were cultured in RPMI 1640 (Invitrogen) supplemented with 10% FCS, 100U/ml pen/ strep.

    Article Title: Tannic Acid-Lung Fluid Assemblies Promote Interaction and Delivery of Drugs to Lung Cancer Cells
    Article Snippet: .. The cancer cell lines cultured under sterile condition in Dulbecco’s Modified Eagle’s medium (DMEM for A549) and Roswell Park Memorial Institute medium (RPMI for H1299) supplemented with 4.5 g/L of glucose, 10 nM of nonessential amino acids (# 11140076, Gibco, Thermo Fisher Scientific, Grand Island, NY, USA), 100 mM of sodium pyruvate (#11360070, Gibco), 1× antibiotic/antimycotic (#15240062, Gibco), and 10% heat-inactivated FBS (#10438026 Thermo Fisher). .. Cells were maintained in 100 mm tissue culture dishes (#83.3902, Sarstedt, Inc., Newton, NC, USA) as 2D monolayers in a humidified incubator (5% CO2 and 95% air condition) at 37 °C (Thermo Fisher Scientific, Waltham, MA, USA).

    Modification:

    Article Title: Tannic Acid-Lung Fluid Assemblies Promote Interaction and Delivery of Drugs to Lung Cancer Cells
    Article Snippet: .. The cancer cell lines cultured under sterile condition in Dulbecco’s Modified Eagle’s medium (DMEM for A549) and Roswell Park Memorial Institute medium (RPMI for H1299) supplemented with 4.5 g/L of glucose, 10 nM of nonessential amino acids (# 11140076, Gibco, Thermo Fisher Scientific, Grand Island, NY, USA), 100 mM of sodium pyruvate (#11360070, Gibco), 1× antibiotic/antimycotic (#15240062, Gibco), and 10% heat-inactivated FBS (#10438026 Thermo Fisher). .. Cells were maintained in 100 mm tissue culture dishes (#83.3902, Sarstedt, Inc., Newton, NC, USA) as 2D monolayers in a humidified incubator (5% CO2 and 95% air condition) at 37 °C (Thermo Fisher Scientific, Waltham, MA, USA).

    Knock-Out:

    Article Title: Analyzing bovine OCT4 and NANOG enhancer activity in pluripotent stem cells using fluorescent protein reporters
    Article Snippet: .. The reprogramming medium was a 1:1 mixture of the KSR-ESC medium containing 20% Knock-out serum replacement (KSR), 1 x MEM non-essential amino acids, 1 x GlutaMax, 0.5 x penicillin/streptomycin, 1 x 2-mercaptoethanol and 1,000 U/mL mLIF in Knock-out DMEM (all from Invitrogen), and the serum-containing ES medium containing 20% ES-FBS, 1 x MEM non-essential amino acids, 1 x GlutaMax, 0.5 x penicillin/streptomycin, 1 x 2-mercaptoethanol and 1,000 U/ml mLIF in DMEM (all from Invitrogen). ..

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    • 1x neaa  (Thermo Fisher)
    97
    Thermo Fisher 1x neaa
    Differentiation of iPSCs into iPS-NSCs. iPSCs were expanded to 80% confluency, and then, the iPSC media were replaced by neuronal induction media (Neurobasal-A, 1X B27-A, 1X N2, 1X NEAA, 1X Glutamax, and 5 mg/mL streptomycin and 5 UI/mL penicillin). The cells were kept in culture until they detached and formed neurospheres. The media containing detached cells were centrifuged, and the pellet was dissociated in Accutase. Cells were then re-plated in neural stem cell media (Neurobasal-A, 1X B27-A, 1X N2, 1X NEAA, 1X Glutamax, 20 ng/mL EGF, 10 ng/mL bFGF, and 5 mg/mL streptomycin and 5 UI/mL penicillin). Cells were passaged three times and then characterized using ICC.
    1x Neaa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x neaa/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x neaa - by Bioz Stars, 2021-03
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Differentiation of iPSCs into iPS-NSCs. iPSCs were expanded to 80% confluency, and then, the iPSC media were replaced by neuronal induction media (Neurobasal-A, 1X B27-A, 1X N2, 1X NEAA, 1X Glutamax, and 5 mg/mL streptomycin and 5 UI/mL penicillin). The cells were kept in culture until they detached and formed neurospheres. The media containing detached cells were centrifuged, and the pellet was dissociated in Accutase. Cells were then re-plated in neural stem cell media (Neurobasal-A, 1X B27-A, 1X N2, 1X NEAA, 1X Glutamax, 20 ng/mL EGF, 10 ng/mL bFGF, and 5 mg/mL streptomycin and 5 UI/mL penicillin). Cells were passaged three times and then characterized using ICC.

    Journal: Frontiers in Neuroscience

    Article Title: Induced Pluripotent Stem Cell-Derived Neural Stem Cell Transplantations Reduced Behavioral Deficits and Ameliorated Neuropathological Changes in YAC128 Mouse Model of Huntington's Disease

    doi: 10.3389/fnins.2017.00628

    Figure Lengend Snippet: Differentiation of iPSCs into iPS-NSCs. iPSCs were expanded to 80% confluency, and then, the iPSC media were replaced by neuronal induction media (Neurobasal-A, 1X B27-A, 1X N2, 1X NEAA, 1X Glutamax, and 5 mg/mL streptomycin and 5 UI/mL penicillin). The cells were kept in culture until they detached and formed neurospheres. The media containing detached cells were centrifuged, and the pellet was dissociated in Accutase. Cells were then re-plated in neural stem cell media (Neurobasal-A, 1X B27-A, 1X N2, 1X NEAA, 1X Glutamax, 20 ng/mL EGF, 10 ng/mL bFGF, and 5 mg/mL streptomycin and 5 UI/mL penicillin). Cells were passaged three times and then characterized using ICC.

    Article Snippet: Briefly, iPSCs were expanded to 80% confluency, and then, the iPSC media were replaced by neuronal induction media [Neurobasal-A (Life Technologies, Carlsbad, CA) supplemented with 1X B27-A (Life Technologies, Carlsbad, CA), 1X N2 (Life Technologies, Carlsbad, CA), 1X NEAA (Life Technologies, Carlsbad, CA), 1X Glutamax (Life Technologies, Carlsbad, CA), and 5 mg/mL streptomycin and 5 UI/mL penicillin].

    Techniques: Immunocytochemistry