1x complete protease inhibitor cocktail roche  (Roche)

 
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    Roche 1x complete protease inhibitor cocktail roche
    1x Complete Protease Inhibitor Cocktail Roche, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x complete protease inhibitor cocktail roche/product/Roche
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x complete protease inhibitor cocktail roche - by Bioz Stars, 2020-03
    78/100 stars

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    Lysis:

    Article Title: Ovarian cancer stem cell like side populations are enriched following chemotherapy and overexpress EZH2
    Article Snippet: .. Cells were re-suspended in lysis buffer (PBS containing 0.5% Triton X 100 (v/v), 1X Complete protease inhibitor cocktail – Roche) and left to lyse for 10 min. at 4°C. ..

    Protease Inhibitor:

    Article Title: Ovarian cancer stem cell like side populations are enriched following chemotherapy and overexpress EZH2
    Article Snippet: .. Cells were re-suspended in lysis buffer (PBS containing 0.5% Triton X 100 (v/v), 1X Complete protease inhibitor cocktail – Roche) and left to lyse for 10 min. at 4°C. ..

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    Roche triton x100 extraction buffer
    Reduction of heparan sulfate biosynthetic function prevents increases of ubiquitin-modified protein in the brains of ROS exposed animals. Accumulation of insoluble ubiquitinated proteins (IUPs) was examined in the <t>triton-X100</t> insoluble fraction of total head proteins. UAS-mCherry RNAi, an shRNAi with no predicted targets in the Drosophila genome, was used as a control. All plus/minus oxidant pairs are from the same blot. All lanes are from the same experiment, with the same standard sample loaded on all gels from this one experiment for standardization (see Supplemental Figure 3 for original blots). Overexpression of Atg8a ( UAS-Atg8a ) was used as a positive control for enhancement of autophagy. The UAS-constructs were expressed under control of elav -GAL4 with UAS-dcrII to enhance RNAi efficacy. Lanes from anti-ubiquitin stained membranes are shown in control vs. oxidant-exposed pairs. The ratio depicted is the average density of anti-ubiquitin staining, normalized to loading, in samples with peroxide (+) divided by samples without peroxide (-). Two different sfl RNAi lines were tested. Two additional replicate experiments gave average peroxide/no peroxide ratios of 1.23 for control, and 0.8 for sfl RNAi-HMS . Two replicates of ttv RNAi also showed lower levels of ubiquitin-insoluble material upon peroxide exposure (ratio of 0.7).
    Triton X100 Extraction Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche complete edta free protease inhibitor cocktail
    Reduction of heparan sulfate biosynthetic function prevents increases of ubiquitin-modified protein in the brains of ROS exposed animals. Accumulation of insoluble ubiquitinated proteins (IUPs) was examined in the <t>triton-X100</t> insoluble fraction of total head proteins. UAS-mCherry RNAi, an shRNAi with no predicted targets in the Drosophila genome, was used as a control. All plus/minus oxidant pairs are from the same blot. All lanes are from the same experiment, with the same standard sample loaded on all gels from this one experiment for standardization (see Supplemental Figure 3 for original blots). Overexpression of Atg8a ( UAS-Atg8a ) was used as a positive control for enhancement of autophagy. The UAS-constructs were expressed under control of elav -GAL4 with UAS-dcrII to enhance RNAi efficacy. Lanes from anti-ubiquitin stained membranes are shown in control vs. oxidant-exposed pairs. The ratio depicted is the average density of anti-ubiquitin staining, normalized to loading, in samples with peroxide (+) divided by samples without peroxide (-). Two different sfl RNAi lines were tested. Two additional replicate experiments gave average peroxide/no peroxide ratios of 1.23 for control, and 0.8 for sfl RNAi-HMS . Two replicates of ttv RNAi also showed lower levels of ubiquitin-insoluble material upon peroxide exposure (ratio of 0.7).
    Complete Edta Free Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete edta free protease inhibitor cocktail/product/Roche
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    Roche im ac buffer
    Reduction of heparan sulfate biosynthetic function prevents increases of ubiquitin-modified protein in the brains of ROS exposed animals. Accumulation of insoluble ubiquitinated proteins (IUPs) was examined in the <t>triton-X100</t> insoluble fraction of total head proteins. UAS-mCherry RNAi, an shRNAi with no predicted targets in the Drosophila genome, was used as a control. All plus/minus oxidant pairs are from the same blot. All lanes are from the same experiment, with the same standard sample loaded on all gels from this one experiment for standardization (see Supplemental Figure 3 for original blots). Overexpression of Atg8a ( UAS-Atg8a ) was used as a positive control for enhancement of autophagy. The UAS-constructs were expressed under control of elav -GAL4 with UAS-dcrII to enhance RNAi efficacy. Lanes from anti-ubiquitin stained membranes are shown in control vs. oxidant-exposed pairs. The ratio depicted is the average density of anti-ubiquitin staining, normalized to loading, in samples with peroxide (+) divided by samples without peroxide (-). Two different sfl RNAi lines were tested. Two additional replicate experiments gave average peroxide/no peroxide ratios of 1.23 for control, and 0.8 for sfl RNAi-HMS . Two replicates of ttv RNAi also showed lower levels of ubiquitin-insoluble material upon peroxide exposure (ratio of 0.7).
    Im Ac Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lysis buffer
    Reduction of heparan sulfate biosynthetic function prevents increases of ubiquitin-modified protein in the brains of ROS exposed animals. Accumulation of insoluble ubiquitinated proteins (IUPs) was examined in the <t>triton-X100</t> insoluble fraction of total head proteins. UAS-mCherry RNAi, an shRNAi with no predicted targets in the Drosophila genome, was used as a control. All plus/minus oxidant pairs are from the same blot. All lanes are from the same experiment, with the same standard sample loaded on all gels from this one experiment for standardization (see Supplemental Figure 3 for original blots). Overexpression of Atg8a ( UAS-Atg8a ) was used as a positive control for enhancement of autophagy. The UAS-constructs were expressed under control of elav -GAL4 with UAS-dcrII to enhance RNAi efficacy. Lanes from anti-ubiquitin stained membranes are shown in control vs. oxidant-exposed pairs. The ratio depicted is the average density of anti-ubiquitin staining, normalized to loading, in samples with peroxide (+) divided by samples without peroxide (-). Two different sfl RNAi lines were tested. Two additional replicate experiments gave average peroxide/no peroxide ratios of 1.23 for control, and 0.8 for sfl RNAi-HMS . Two replicates of ttv RNAi also showed lower levels of ubiquitin-insoluble material upon peroxide exposure (ratio of 0.7).
    Lysis Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1428 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Reduction of heparan sulfate biosynthetic function prevents increases of ubiquitin-modified protein in the brains of ROS exposed animals. Accumulation of insoluble ubiquitinated proteins (IUPs) was examined in the triton-X100 insoluble fraction of total head proteins. UAS-mCherry RNAi, an shRNAi with no predicted targets in the Drosophila genome, was used as a control. All plus/minus oxidant pairs are from the same blot. All lanes are from the same experiment, with the same standard sample loaded on all gels from this one experiment for standardization (see Supplemental Figure 3 for original blots). Overexpression of Atg8a ( UAS-Atg8a ) was used as a positive control for enhancement of autophagy. The UAS-constructs were expressed under control of elav -GAL4 with UAS-dcrII to enhance RNAi efficacy. Lanes from anti-ubiquitin stained membranes are shown in control vs. oxidant-exposed pairs. The ratio depicted is the average density of anti-ubiquitin staining, normalized to loading, in samples with peroxide (+) divided by samples without peroxide (-). Two different sfl RNAi lines were tested. Two additional replicate experiments gave average peroxide/no peroxide ratios of 1.23 for control, and 0.8 for sfl RNAi-HMS . Two replicates of ttv RNAi also showed lower levels of ubiquitin-insoluble material upon peroxide exposure (ratio of 0.7).

    Journal: G3: Genes|Genomes|Genetics

    Article Title: Heparan Sulfate Structure Affects Autophagy, Lifespan, Responses to Oxidative Stress, and Cell Degeneration in Drosophila parkin Mutants

    doi: 10.1534/g3.119.400730

    Figure Lengend Snippet: Reduction of heparan sulfate biosynthetic function prevents increases of ubiquitin-modified protein in the brains of ROS exposed animals. Accumulation of insoluble ubiquitinated proteins (IUPs) was examined in the triton-X100 insoluble fraction of total head proteins. UAS-mCherry RNAi, an shRNAi with no predicted targets in the Drosophila genome, was used as a control. All plus/minus oxidant pairs are from the same blot. All lanes are from the same experiment, with the same standard sample loaded on all gels from this one experiment for standardization (see Supplemental Figure 3 for original blots). Overexpression of Atg8a ( UAS-Atg8a ) was used as a positive control for enhancement of autophagy. The UAS-constructs were expressed under control of elav -GAL4 with UAS-dcrII to enhance RNAi efficacy. Lanes from anti-ubiquitin stained membranes are shown in control vs. oxidant-exposed pairs. The ratio depicted is the average density of anti-ubiquitin staining, normalized to loading, in samples with peroxide (+) divided by samples without peroxide (-). Two different sfl RNAi lines were tested. Two additional replicate experiments gave average peroxide/no peroxide ratios of 1.23 for control, and 0.8 for sfl RNAi-HMS . Two replicates of ttv RNAi also showed lower levels of ubiquitin-insoluble material upon peroxide exposure (ratio of 0.7).

    Article Snippet: Typically, 30-60 adult heads were homogenized by ceramic bead agitation using the Bead Ruptor 24 in 150ul of Triton-X100 extraction buffer (1% Triton-X100, 1x PBS, 1X protease inhibitor cocktail Complete [Roche, 10184600]).

    Techniques: Modification, Over Expression, Positive Control, Construct, Staining