1x cell lysis buffer  (Cell Signaling Technology Inc)

 
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    Name:
    Cell Lysis Buffer 10X
    Description:
    Cell Lysis Buffer is used to lyse cells under nondenaturing conditions
    Catalog Number:
    9803
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    Category:
    Buffers Dyes
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    Structured Review

    Cell Signaling Technology Inc 1x cell lysis buffer
    Survivin expression profiles in Y79-Luc (A) and CHLA-215-Luc (B) tumors growing in the vitreal cavity of mice. Mice were treated with carboplatin (CBDCA, 60 mg/kg) via IP injection on days 15 and 18 following transplantation, YM155 alone (2mg/kg) administered via IP injection for 5 consecutive days starting 14 days post transplantation, or the combination of CBDCA and YM155. Eyes were enucleated 24 hours following the last treatment, homogenized with a polytron in <t>1X</t> cell lysis buffer, and analyzed for survivin expression by Western immunoblot and densitometry. Upper figure shows a representative Western immunoblot and lower figure provides quantitative densitometric values from all immunoblots. Columns , mean; bars , ± SE of three to nine separate intraocular lesions. ** P
    Cell Lysis Buffer is used to lyse cells under nondenaturing conditions
    https://www.bioz.com/result/1x cell lysis buffer/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x cell lysis buffer - by Bioz Stars, 2021-07
    99/100 stars

    Images

    1) Product Images from "Targeting Survivin Enhances Chemosensitivity in Retinoblastoma Cells and Orthotopic Tumors"

    Article Title: Targeting Survivin Enhances Chemosensitivity in Retinoblastoma Cells and Orthotopic Tumors

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0153011

    Survivin expression profiles in Y79-Luc (A) and CHLA-215-Luc (B) tumors growing in the vitreal cavity of mice. Mice were treated with carboplatin (CBDCA, 60 mg/kg) via IP injection on days 15 and 18 following transplantation, YM155 alone (2mg/kg) administered via IP injection for 5 consecutive days starting 14 days post transplantation, or the combination of CBDCA and YM155. Eyes were enucleated 24 hours following the last treatment, homogenized with a polytron in 1X cell lysis buffer, and analyzed for survivin expression by Western immunoblot and densitometry. Upper figure shows a representative Western immunoblot and lower figure provides quantitative densitometric values from all immunoblots. Columns , mean; bars , ± SE of three to nine separate intraocular lesions. ** P
    Figure Legend Snippet: Survivin expression profiles in Y79-Luc (A) and CHLA-215-Luc (B) tumors growing in the vitreal cavity of mice. Mice were treated with carboplatin (CBDCA, 60 mg/kg) via IP injection on days 15 and 18 following transplantation, YM155 alone (2mg/kg) administered via IP injection for 5 consecutive days starting 14 days post transplantation, or the combination of CBDCA and YM155. Eyes were enucleated 24 hours following the last treatment, homogenized with a polytron in 1X cell lysis buffer, and analyzed for survivin expression by Western immunoblot and densitometry. Upper figure shows a representative Western immunoblot and lower figure provides quantitative densitometric values from all immunoblots. Columns , mean; bars , ± SE of three to nine separate intraocular lesions. ** P

    Techniques Used: Expressing, Mouse Assay, Injection, Transplantation Assay, Lysis, Western Blot

    2) Product Images from "Targeting Survivin Enhances Chemosensitivity in Retinoblastoma Cells and Orthotopic Tumors"

    Article Title: Targeting Survivin Enhances Chemosensitivity in Retinoblastoma Cells and Orthotopic Tumors

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0153011

    Survivin expression profiles in Y79-Luc (A) and CHLA-215-Luc (B) tumors growing in the vitreal cavity of mice. Mice were treated with carboplatin (CBDCA, 60 mg/kg) via IP injection on days 15 and 18 following transplantation, YM155 alone (2mg/kg) administered via IP injection for 5 consecutive days starting 14 days post transplantation, or the combination of CBDCA and YM155. Eyes were enucleated 24 hours following the last treatment, homogenized with a polytron in 1X cell lysis buffer, and analyzed for survivin expression by Western immunoblot and densitometry. Upper figure shows a representative Western immunoblot and lower figure provides quantitative densitometric values from all immunoblots. Columns , mean; bars , ± SE of three to nine separate intraocular lesions. ** P
    Figure Legend Snippet: Survivin expression profiles in Y79-Luc (A) and CHLA-215-Luc (B) tumors growing in the vitreal cavity of mice. Mice were treated with carboplatin (CBDCA, 60 mg/kg) via IP injection on days 15 and 18 following transplantation, YM155 alone (2mg/kg) administered via IP injection for 5 consecutive days starting 14 days post transplantation, or the combination of CBDCA and YM155. Eyes were enucleated 24 hours following the last treatment, homogenized with a polytron in 1X cell lysis buffer, and analyzed for survivin expression by Western immunoblot and densitometry. Upper figure shows a representative Western immunoblot and lower figure provides quantitative densitometric values from all immunoblots. Columns , mean; bars , ± SE of three to nine separate intraocular lesions. ** P

    Techniques Used: Expressing, Mouse Assay, Injection, Transplantation Assay, Lysis, Western Blot

    Related Articles

    Protein Extraction:

    Article Title: Knocking Down TMPRSS2-ERG Fusion Oncogene by siRNA Could be an Alternative Treatment to Flutamide
    Article Snippet: .. Total protein extraction using M-PER lysis buffer and western blot were performed for ERG, cleaved caspase-3 (# 9662; 1:1,000; Cell Signaling Technology) and GAPDH as described above. .. Knowing that FLU prevents nuclear shuttling of AR, nuclear protein extraction was achieved as described by Erin et al ., then western blot were performed using AR antibody (ab108341; 1:1,000, Abcam Biochemicals).

    Lysis:

    Article Title: Knocking Down TMPRSS2-ERG Fusion Oncogene by siRNA Could be an Alternative Treatment to Flutamide
    Article Snippet: .. Total protein extraction using M-PER lysis buffer and western blot were performed for ERG, cleaved caspase-3 (# 9662; 1:1,000; Cell Signaling Technology) and GAPDH as described above. .. Knowing that FLU prevents nuclear shuttling of AR, nuclear protein extraction was achieved as described by Erin et al ., then western blot were performed using AR antibody (ab108341; 1:1,000, Abcam Biochemicals).

    Article Title: Hes1 is associated with long non-coding RNAs in colorectal cancer
    Article Snippet: We carried out RIP using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, MD, USA) following the manufacturer’s instructions. .. Cells were lysed in RIP Lysis Buffer, and immunoprecipitated with Hes1 antibody (CST, MA, USA) to RNA-binding protein of interest with protein A/G magnetic beads. .. RNAs were extracted and analyzed by RT-PCR or Illumina HiSeq 2500 Sequencing.

    Article Title: Depleting deubiquitinating enzymes promotes apoptosis in glioma cell line via RNA binding proteins SF2/ASF1
    Article Snippet: In another experiment T98G cells were transfected next day after plating with two different specific siRNAs (Eurogentec) against hnRNPA1 (10 nM). .. Protein lysates were prepared after 72 h of transfection in cell lysis buffer (Cell Signaling Technology) containing protease inhibitor (Abcam) and phosphatase inhibitor (Santa Cruz Biotechnology, Inc.). .. In all experiment siRNA mediated knock down efficiency was achieved ≥50% to 60% using Interferrin transfection reagent from Polyplus Company.

    Article Title: Long non-coding RNA HOTAIR acts as a competing endogenous RNA to promote malignant melanoma progression by sponging miR-152-3p
    Article Snippet: RNA immunoprecipitation (RIP) RIP assay was performed using the EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA). .. The melanoma cells were scraped off and lysed in complete RIP lysis buffer, and 100 μl of whole cell extract were incubated with RIP buffer containing magnetic beads conjugated with human anti-Ago2 antibody (Cell Signaling, USA). .. Positive control is SNRNP70 (Millipore, USA) and negative control is normal mouse IgG (Millipore, USA).

    Article Title: Long non-coding RNA CARLo-5 promotes tumor progression in hepatocellular carcinoma via suppressing miR-200b expression
    Article Snippet: RNA binding protein immunoprecipitation (RIP) assay RNA immunoprecipitation was performed using the EZMagna RIP kit (Millipore, Billerica, MA, USA) according to the manufacturer’s protocol. .. MHCC97H and MHCC97L cells at 80-90% confluency were scraped off, then lysed in complete RIP lysis buffer, after which 100 μl of whole cell extract was incubated with RIP buffer containing magnetic beads conjugated with human anti-EZH2 and anti-H3k27me3 antibody (Cell Signaling Technology, USA), negative control normal mouse IgG (Millipore). .. Anti-SNRNP70 (Millipore) was used as positive control for the RIP procedure.

    Western Blot:

    Article Title: Knocking Down TMPRSS2-ERG Fusion Oncogene by siRNA Could be an Alternative Treatment to Flutamide
    Article Snippet: .. Total protein extraction using M-PER lysis buffer and western blot were performed for ERG, cleaved caspase-3 (# 9662; 1:1,000; Cell Signaling Technology) and GAPDH as described above. .. Knowing that FLU prevents nuclear shuttling of AR, nuclear protein extraction was achieved as described by Erin et al ., then western blot were performed using AR antibody (ab108341; 1:1,000, Abcam Biochemicals).

    Immunoprecipitation:

    Article Title: Hes1 is associated with long non-coding RNAs in colorectal cancer
    Article Snippet: We carried out RIP using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, MD, USA) following the manufacturer’s instructions. .. Cells were lysed in RIP Lysis Buffer, and immunoprecipitated with Hes1 antibody (CST, MA, USA) to RNA-binding protein of interest with protein A/G magnetic beads. .. RNAs were extracted and analyzed by RT-PCR or Illumina HiSeq 2500 Sequencing.

    RNA Binding Assay:

    Article Title: Hes1 is associated with long non-coding RNAs in colorectal cancer
    Article Snippet: We carried out RIP using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, MD, USA) following the manufacturer’s instructions. .. Cells were lysed in RIP Lysis Buffer, and immunoprecipitated with Hes1 antibody (CST, MA, USA) to RNA-binding protein of interest with protein A/G magnetic beads. .. RNAs were extracted and analyzed by RT-PCR or Illumina HiSeq 2500 Sequencing.

    Magnetic Beads:

    Article Title: Hes1 is associated with long non-coding RNAs in colorectal cancer
    Article Snippet: We carried out RIP using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, MD, USA) following the manufacturer’s instructions. .. Cells were lysed in RIP Lysis Buffer, and immunoprecipitated with Hes1 antibody (CST, MA, USA) to RNA-binding protein of interest with protein A/G magnetic beads. .. RNAs were extracted and analyzed by RT-PCR or Illumina HiSeq 2500 Sequencing.

    Article Title: Long non-coding RNA HOXA11-AS promotes the proliferation HCC cells by epigenetically silencing DUSP5
    Article Snippet: Cells at 80–90% confluency were scraped off and lysed in complete RIP lysis buffer. .. Then, 100 μl of whole cell extract was incubated with RIP buffer containing magnetic beads conjugated with human anti-EZH2 antibody (Cell Signaling, USA), anti-SUZ12 antibody (Cell Signaling, USA), anti-EED antibody (Cell Signaling, USA) negative control normal mouse IgG (Millipore). .. ChIP assay Chromatin immunoprecipitation (ChIP) assays were performed using the Millipore ChIP Assay Kit (catalogno.

    Article Title: Long non-coding RNA HOTAIR acts as a competing endogenous RNA to promote malignant melanoma progression by sponging miR-152-3p
    Article Snippet: RNA immunoprecipitation (RIP) RIP assay was performed using the EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA). .. The melanoma cells were scraped off and lysed in complete RIP lysis buffer, and 100 μl of whole cell extract were incubated with RIP buffer containing magnetic beads conjugated with human anti-Ago2 antibody (Cell Signaling, USA). .. Positive control is SNRNP70 (Millipore, USA) and negative control is normal mouse IgG (Millipore, USA).

    Article Title: Long non-coding RNA CARLo-5 promotes tumor progression in hepatocellular carcinoma via suppressing miR-200b expression
    Article Snippet: RNA binding protein immunoprecipitation (RIP) assay RNA immunoprecipitation was performed using the EZMagna RIP kit (Millipore, Billerica, MA, USA) according to the manufacturer’s protocol. .. MHCC97H and MHCC97L cells at 80-90% confluency were scraped off, then lysed in complete RIP lysis buffer, after which 100 μl of whole cell extract was incubated with RIP buffer containing magnetic beads conjugated with human anti-EZH2 and anti-H3k27me3 antibody (Cell Signaling Technology, USA), negative control normal mouse IgG (Millipore). .. Anti-SNRNP70 (Millipore) was used as positive control for the RIP procedure.

    Transfection:

    Article Title: Depleting deubiquitinating enzymes promotes apoptosis in glioma cell line via RNA binding proteins SF2/ASF1
    Article Snippet: In another experiment T98G cells were transfected next day after plating with two different specific siRNAs (Eurogentec) against hnRNPA1 (10 nM). .. Protein lysates were prepared after 72 h of transfection in cell lysis buffer (Cell Signaling Technology) containing protease inhibitor (Abcam) and phosphatase inhibitor (Santa Cruz Biotechnology, Inc.). .. In all experiment siRNA mediated knock down efficiency was achieved ≥50% to 60% using Interferrin transfection reagent from Polyplus Company.

    Protease Inhibitor:

    Article Title: Depleting deubiquitinating enzymes promotes apoptosis in glioma cell line via RNA binding proteins SF2/ASF1
    Article Snippet: In another experiment T98G cells were transfected next day after plating with two different specific siRNAs (Eurogentec) against hnRNPA1 (10 nM). .. Protein lysates were prepared after 72 h of transfection in cell lysis buffer (Cell Signaling Technology) containing protease inhibitor (Abcam) and phosphatase inhibitor (Santa Cruz Biotechnology, Inc.). .. In all experiment siRNA mediated knock down efficiency was achieved ≥50% to 60% using Interferrin transfection reagent from Polyplus Company.

    Incubation:

    Article Title: Long non-coding RNA HOXA11-AS promotes the proliferation HCC cells by epigenetically silencing DUSP5
    Article Snippet: Cells at 80–90% confluency were scraped off and lysed in complete RIP lysis buffer. .. Then, 100 μl of whole cell extract was incubated with RIP buffer containing magnetic beads conjugated with human anti-EZH2 antibody (Cell Signaling, USA), anti-SUZ12 antibody (Cell Signaling, USA), anti-EED antibody (Cell Signaling, USA) negative control normal mouse IgG (Millipore). .. ChIP assay Chromatin immunoprecipitation (ChIP) assays were performed using the Millipore ChIP Assay Kit (catalogno.

    Article Title: A non-canonical DNA structure is a binding motif for the transcription factor SP1 in vitro
    Article Snippet: For detection, 50 μl of a rabbit polyclonal SP1 antibody (Millipore) at 1:500 dilution in buffer Z were added per well and incubated for 1 h at room temperature. .. After washing three times with buffer Z, a HRP-conjugated goat anti-rabbit (Cell Signalling Technology) diluted 1:2000 in buffer Z with 3% BSA was added and incubated for 45 min at room temperature. .. Wells were washed three times with buffer Z and peroxidase activity detected by adding 100 μl of the BM blue POD substrate (Roche).

    Article Title: Long non-coding RNA HOTAIR acts as a competing endogenous RNA to promote malignant melanoma progression by sponging miR-152-3p
    Article Snippet: RNA immunoprecipitation (RIP) RIP assay was performed using the EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA). .. The melanoma cells were scraped off and lysed in complete RIP lysis buffer, and 100 μl of whole cell extract were incubated with RIP buffer containing magnetic beads conjugated with human anti-Ago2 antibody (Cell Signaling, USA). .. Positive control is SNRNP70 (Millipore, USA) and negative control is normal mouse IgG (Millipore, USA).

    Article Title: Long non-coding RNA CARLo-5 promotes tumor progression in hepatocellular carcinoma via suppressing miR-200b expression
    Article Snippet: RNA binding protein immunoprecipitation (RIP) assay RNA immunoprecipitation was performed using the EZMagna RIP kit (Millipore, Billerica, MA, USA) according to the manufacturer’s protocol. .. MHCC97H and MHCC97L cells at 80-90% confluency were scraped off, then lysed in complete RIP lysis buffer, after which 100 μl of whole cell extract was incubated with RIP buffer containing magnetic beads conjugated with human anti-EZH2 and anti-H3k27me3 antibody (Cell Signaling Technology, USA), negative control normal mouse IgG (Millipore). .. Anti-SNRNP70 (Millipore) was used as positive control for the RIP procedure.

    Negative Control:

    Article Title: Long non-coding RNA HOXA11-AS promotes the proliferation HCC cells by epigenetically silencing DUSP5
    Article Snippet: Cells at 80–90% confluency were scraped off and lysed in complete RIP lysis buffer. .. Then, 100 μl of whole cell extract was incubated with RIP buffer containing magnetic beads conjugated with human anti-EZH2 antibody (Cell Signaling, USA), anti-SUZ12 antibody (Cell Signaling, USA), anti-EED antibody (Cell Signaling, USA) negative control normal mouse IgG (Millipore). .. ChIP assay Chromatin immunoprecipitation (ChIP) assays were performed using the Millipore ChIP Assay Kit (catalogno.

    Article Title: Long non-coding RNA CARLo-5 promotes tumor progression in hepatocellular carcinoma via suppressing miR-200b expression
    Article Snippet: RNA binding protein immunoprecipitation (RIP) assay RNA immunoprecipitation was performed using the EZMagna RIP kit (Millipore, Billerica, MA, USA) according to the manufacturer’s protocol. .. MHCC97H and MHCC97L cells at 80-90% confluency were scraped off, then lysed in complete RIP lysis buffer, after which 100 μl of whole cell extract was incubated with RIP buffer containing magnetic beads conjugated with human anti-EZH2 and anti-H3k27me3 antibody (Cell Signaling Technology, USA), negative control normal mouse IgG (Millipore). .. Anti-SNRNP70 (Millipore) was used as positive control for the RIP procedure.

    SDS Page:

    Article Title: Actin-Modulating Protein Cofilin Is Involved in the Formation of Measles Virus Ribonucleoprotein Complex at the Perinuclear Region
    Article Snippet: To detect phosphorylated cofilin, subconfluent monolayers of HeLa/hSLAM cells on 24-well plates were infected with IC323 for 1 h at 37°C, washed with PBS, and cultured with complete DMEM for the indicated lengths of time. .. Cells were lysed in 1× SDS loading buffer, subjected to SDS-PAGE, and subsequently examined by blot analysis using antibody against phosphorylated cofilin (77G2; Cell Signaling Technology) or total cofilin (ab42824; Abcam). .. Tubulin (sc-5286; Santa Cruz Biotechnology) was used as a loading control.

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  • 97
    Cell Signaling Technology Inc pathscan sandwich elisa assay
    LiCl enhances inhibitory GSK-3β phosphorylation in human RMS cell lines. <t>PathScan</t> ® <t>ELISA</t> assays detecting total and phospho-GSK-3β (serine 9) were performed after 36 h of single or combined treatment with 1 μM ATO and 25 mM LiCl in three RMS cell lines in quadruplicate. Mock treated control was set to 100% GSK-3β abundance, respectively phosphorylation. The relative phosphorylation was calculated by normalizing GSK-3β phosphorylation to total GSK-3β. Error bars indicate the standard deviation. Significant differences between groups are indicated by small letters. Multiplicity adjusted p values for each treatment against mock treated control were determined: “a” indicates p ≤ 0.0001, “b” indicates p ≤ 0.05, “c” indicates p ≤ 0.01. In addition, p values for combined treatment relative to single treatment were determined: “d” indicates p ≤ 0.0001.
    Pathscan Sandwich Elisa Assay, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pathscan sandwich elisa assay/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pathscan sandwich elisa assay - by Bioz Stars, 2021-07
    97/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc 1x cell lysis buffer
    Survivin expression profiles in Y79-Luc (A) and CHLA-215-Luc (B) tumors growing in the vitreal cavity of mice. Mice were treated with carboplatin (CBDCA, 60 mg/kg) via IP injection on days 15 and 18 following transplantation, YM155 alone (2mg/kg) administered via IP injection for 5 consecutive days starting 14 days post transplantation, or the combination of CBDCA and YM155. Eyes were enucleated 24 hours following the last treatment, homogenized with a polytron in <t>1X</t> cell lysis buffer, and analyzed for survivin expression by Western immunoblot and densitometry. Upper figure shows a representative Western immunoblot and lower figure provides quantitative densitometric values from all immunoblots. Columns , mean; bars , ± SE of three to nine separate intraocular lesions. ** P
    1x Cell Lysis Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x cell lysis buffer/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x cell lysis buffer - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier

    97
    Cell Signaling Technology Inc control pbs
    <t>WPF-induced</t> activation of EGFR-ERK pathway. Human gastric epithelial cell line (GES-1) cells were treated with control <t>(PBS)</t> or WPF (1.3 mg/mL) for 12 h before protein was extracted by cell lysis buffer and analyzed using Western blot. GAPDH was used as a loading control. ( A ) Representative blots of p-ERK, ERK, p-EGFR and EGFR; ( B ) Changes of p-ERK/ERK; ( C ) Changes of p-EGFR/EGFR. Data are presented as mean ± SD. * p
    Control Pbs, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control pbs/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    control pbs - by Bioz Stars, 2021-07
    97/100 stars
      Buy from Supplier

    Image Search Results


    LiCl enhances inhibitory GSK-3β phosphorylation in human RMS cell lines. PathScan ® ELISA assays detecting total and phospho-GSK-3β (serine 9) were performed after 36 h of single or combined treatment with 1 μM ATO and 25 mM LiCl in three RMS cell lines in quadruplicate. Mock treated control was set to 100% GSK-3β abundance, respectively phosphorylation. The relative phosphorylation was calculated by normalizing GSK-3β phosphorylation to total GSK-3β. Error bars indicate the standard deviation. Significant differences between groups are indicated by small letters. Multiplicity adjusted p values for each treatment against mock treated control were determined: “a” indicates p ≤ 0.0001, “b” indicates p ≤ 0.05, “c” indicates p ≤ 0.01. In addition, p values for combined treatment relative to single treatment were determined: “d” indicates p ≤ 0.0001.

    Journal: PLoS ONE

    Article Title: Combined application of arsenic trioxide and lithium chloride augments viability reduction and apoptosis induction in human rhabdomyosarcoma cell lines

    doi: 10.1371/journal.pone.0178857

    Figure Lengend Snippet: LiCl enhances inhibitory GSK-3β phosphorylation in human RMS cell lines. PathScan ® ELISA assays detecting total and phospho-GSK-3β (serine 9) were performed after 36 h of single or combined treatment with 1 μM ATO and 25 mM LiCl in three RMS cell lines in quadruplicate. Mock treated control was set to 100% GSK-3β abundance, respectively phosphorylation. The relative phosphorylation was calculated by normalizing GSK-3β phosphorylation to total GSK-3β. Error bars indicate the standard deviation. Significant differences between groups are indicated by small letters. Multiplicity adjusted p values for each treatment against mock treated control were determined: “a” indicates p ≤ 0.0001, “b” indicates p ≤ 0.05, “c” indicates p ≤ 0.01. In addition, p values for combined treatment relative to single treatment were determined: “d” indicates p ≤ 0.0001.

    Article Snippet: PathScan® sandwich ELISA assay 0.5 x 106 RD, SRH, RH-30 and SKMC cells were incubated with inhibitor concentrations indicated for 36 h in 6-well-plates and the PathScan® ELISA (Cell Signaling Technology, Leiden, Netherlands) specific for phospho-GSK-3β (serine 9) ♯7311C and total GSK-3β ♯7265C was carried out according the manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation

    Survivin expression profiles in Y79-Luc (A) and CHLA-215-Luc (B) tumors growing in the vitreal cavity of mice. Mice were treated with carboplatin (CBDCA, 60 mg/kg) via IP injection on days 15 and 18 following transplantation, YM155 alone (2mg/kg) administered via IP injection for 5 consecutive days starting 14 days post transplantation, or the combination of CBDCA and YM155. Eyes were enucleated 24 hours following the last treatment, homogenized with a polytron in 1X cell lysis buffer, and analyzed for survivin expression by Western immunoblot and densitometry. Upper figure shows a representative Western immunoblot and lower figure provides quantitative densitometric values from all immunoblots. Columns , mean; bars , ± SE of three to nine separate intraocular lesions. ** P

    Journal: PLoS ONE

    Article Title: Targeting Survivin Enhances Chemosensitivity in Retinoblastoma Cells and Orthotopic Tumors

    doi: 10.1371/journal.pone.0153011

    Figure Lengend Snippet: Survivin expression profiles in Y79-Luc (A) and CHLA-215-Luc (B) tumors growing in the vitreal cavity of mice. Mice were treated with carboplatin (CBDCA, 60 mg/kg) via IP injection on days 15 and 18 following transplantation, YM155 alone (2mg/kg) administered via IP injection for 5 consecutive days starting 14 days post transplantation, or the combination of CBDCA and YM155. Eyes were enucleated 24 hours following the last treatment, homogenized with a polytron in 1X cell lysis buffer, and analyzed for survivin expression by Western immunoblot and densitometry. Upper figure shows a representative Western immunoblot and lower figure provides quantitative densitometric values from all immunoblots. Columns , mean; bars , ± SE of three to nine separate intraocular lesions. ** P

    Article Snippet: Cells were collected at specified times and sonicated in 1X cell lysis buffer (Cell Signaling Technology, Danvers, MA) containing 1μM phenylmethylsulfonyl fluoride (Sigma, St. Louis, MO).

    Techniques: Expressing, Mouse Assay, Injection, Transplantation Assay, Lysis, Western Blot

    WPF-induced activation of EGFR-ERK pathway. Human gastric epithelial cell line (GES-1) cells were treated with control (PBS) or WPF (1.3 mg/mL) for 12 h before protein was extracted by cell lysis buffer and analyzed using Western blot. GAPDH was used as a loading control. ( A ) Representative blots of p-ERK, ERK, p-EGFR and EGFR; ( B ) Changes of p-ERK/ERK; ( C ) Changes of p-EGFR/EGFR. Data are presented as mean ± SD. * p

    Journal: Nutrients

    Article Title: A Novel Combination of Wheat Peptides and Fucoidan Attenuates Ethanol-Induced Gastric Mucosal Damage through Anti-Oxidant, Anti-Inflammatory, and Pro-Survival Mechanisms

    doi: 10.3390/nu9090978

    Figure Lengend Snippet: WPF-induced activation of EGFR-ERK pathway. Human gastric epithelial cell line (GES-1) cells were treated with control (PBS) or WPF (1.3 mg/mL) for 12 h before protein was extracted by cell lysis buffer and analyzed using Western blot. GAPDH was used as a loading control. ( A ) Representative blots of p-ERK, ERK, p-EGFR and EGFR; ( B ) Changes of p-ERK/ERK; ( C ) Changes of p-EGFR/EGFR. Data are presented as mean ± SD. * p

    Article Snippet: Western Blot GES-1 cells were treated with control (PBS) or WPF (1.3 mg/mL) for 12 h before protein extraction with cell lysis buffer (Cell Signaling, Danvers, MA, USA).

    Techniques: Activation Assay, Lysis, Western Blot