Journal: Nutrition & Metabolism

Article Title: Angiotensin(1–7) attenuates visceral adipose tissue expansion and lipogenesis by suppression of endoplasmic reticulum stress via Mas receptor

doi: 10.1186/s12986-022-00716-x

Figure Lengend Snippet: ACE2 deletion induced adipocyte hypertrophy, increased lipogenesis and ER stress related markers in epididymal adipose tissue in mice. A Hematoxylin/eosin staining for epididymal adipose tissue from WT and ACE2 KO mice. Bars indicate a length of 50um. B The mean diameter of epididymal adipocytes. C and D Representative Western blot and relative protein levels of lipogenesis related markers in epididymal adipose tissue. E and F Representative Western blot and relative protein levels of ER stress related markers in epididymal adipose tissue. Data are represented as mean ± SEM from n = 3. * p < 0.05, ** p < 0.01, *** P < 0.001vs WT group. WT, wild-type; ACE2, angiotensin-converting enzyme 2; KO, knock out; FAS, fatty acid synthase; ACCα, acetyl-CoA carboxylase α; M-SREBP-1c, mature sterol regulatory element-binding protein-1c; GRP78, glucose regulated protein 78; ATF4, activating transcription factor 4; CHOP, C/EBP homologous protein

Article Snippet: The antibodies targeted the following proteins: β-Actin (4970, CST, USA), ACE2 (Sc-20998, Santa Cruz, USA), Mas (AAR-013, Alomone labs, Israel), Fatty Acid Synthase (FAS) (3180, CST), acetyl-CoA carboxylase α (ACCα) (Sc-30212, Santa Cruz), sterol regulatory element-binding protein-1c (SREBP-1c) (Sc-366, Santa Cruz), C/EBP homologous protein (CHOP) (2895, CST), Glucose regulated protein 78 (GRP78) (ab21685, Abcam, England), activating transcription factor 4 (ATF4) (11,815, CST).

Techniques: Staining, Western Blot, Knock-Out, Binding Assay

Journal: Nutrition & Metabolism

Article Title: Angiotensin(1–7) attenuates visceral adipose tissue expansion and lipogenesis by suppression of endoplasmic reticulum stress via Mas receptor

doi: 10.1186/s12986-022-00716-x

Figure Lengend Snippet: Mas deletion induced adipocyte hypertrophy, increased lipogenesis and ER stress related markers in epididymal adipose tissue in mice. A Hematoxylin/eosin staining for epididymal adipose tissue from WT and Mas KO mice. Bars indicate a length of 50um. B The mean diameter of epididymal adipocytes. C and D Representative Western blot and relative protein levels of lipogenesis related markers in epididymal adipose tissue. E and F Representative Western blot and relative protein levels of ER stress related markers in epididymal adipose tissue. Data are represented as mean ± SEM from n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001 versus WT group. WT, wild-type; KO, knock out; FAS, fatty acid synthase; ACCα, acetyl-CoA carboxylase α; M-SREBP-1c, mature sterol regulatory element-binding protein-1c; GRP78, glucose regulated protein 78; ATF4, activating transcription factor 4; CHOP, C/EBP homologous protein

Article Snippet: The antibodies targeted the following proteins: β-Actin (4970, CST, USA), ACE2 (Sc-20998, Santa Cruz, USA), Mas (AAR-013, Alomone labs, Israel), Fatty Acid Synthase (FAS) (3180, CST), acetyl-CoA carboxylase α (ACCα) (Sc-30212, Santa Cruz), sterol regulatory element-binding protein-1c (SREBP-1c) (Sc-366, Santa Cruz), C/EBP homologous protein (CHOP) (2895, CST), Glucose regulated protein 78 (GRP78) (ab21685, Abcam, England), activating transcription factor 4 (ATF4) (11,815, CST).

Techniques: Staining, Western Blot, Knock-Out, Binding Assay

Journal: Nutrition & Metabolism

Article Title: Angiotensin(1–7) attenuates visceral adipose tissue expansion and lipogenesis by suppression of endoplasmic reticulum stress via Mas receptor

doi: 10.1186/s12986-022-00716-x

Figure Lengend Snippet: Ang(1–7) treatment induced decreased adipocytes size, lipogenesis and ER stress related markers in epididymal adipose tissue of db/db mice. A Hematoxylin/eosin staining for epididymal adipose tissue from db/db mice treated with NS, Ang(1–7), and Ang(1–7) combined with A779. Bars indicate a length of 50um. B The mean diameter of epididymal adipocytes. C and D Representative Western blot and relative protein levels of lipogenesis related markers in epididymal adipose tissue. E and F Representative Western blot and relative protein levels of ER stress related markers in epididymal adipose tissue. Data are represented as mean ± SEM from n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001 versus NS group; # p < 0.05, ## p < 0.01, ### p < 0.001 versus Ang(1–7) group by one-way ANOVA. Ang(1–7), angiotensin(1–7); NS, normal saline; FAS, fatty acid synthase; ACCα, acetyl-CoA carboxylase α; M-SREBP-1c, mature sterol regulatory element-binding protein-1c; GRP78, glucose regulated protein 78; ATF4, activating transcription factor 4;CHOP, C/EBP homologous protein

Article Snippet: The antibodies targeted the following proteins: β-Actin (4970, CST, USA), ACE2 (Sc-20998, Santa Cruz, USA), Mas (AAR-013, Alomone labs, Israel), Fatty Acid Synthase (FAS) (3180, CST), acetyl-CoA carboxylase α (ACCα) (Sc-30212, Santa Cruz), sterol regulatory element-binding protein-1c (SREBP-1c) (Sc-366, Santa Cruz), C/EBP homologous protein (CHOP) (2895, CST), Glucose regulated protein 78 (GRP78) (ab21685, Abcam, England), activating transcription factor 4 (ATF4) (11,815, CST).

Techniques: Staining, Western Blot, Binding Assay

Journal: Nutrition & Metabolism

Article Title: Angiotensin(1–7) attenuates visceral adipose tissue expansion and lipogenesis by suppression of endoplasmic reticulum stress via Mas receptor

doi: 10.1186/s12986-022-00716-x

Figure Lengend Snippet: Ang(1–7) treatment induced decreased lipogenesis and ER stress related markers in differentiated 3T3-L1 cells pre-loaded with palmitic acid. The differentiated cells were pre-loaded with 400 µM of palmitic acid for 24 h to induce ER stress, then treated with NS, 10 –9 mmol/L Ang(1–7) or both Ang(1–7) and 10 –6 mmol/L A779 for 24 h. A and B Representative Western blot and relative protein levels of lipogenesis related markers. C and D Representative Western blot and relative protein levels of ER stress related markers. Data are represented as mean ± SEM. * p < 0.05 versus NS group; # p < 0.05 versus Ang(1–7) group by one-way ANOVA. Ang(1–7), angiotensin(1–7); NS, normal saline; FAS, fatty acid synthase; ACCα, acetyl-CoA carboxylase α; M-SREBP-1c, mature sterol regulatory element-binding protein-1c; GRP78, glucose regulated protein 78; ATF4, activating transcription factor 4; CHOP, C/EBP homologous protein

Article Snippet: The antibodies targeted the following proteins: β-Actin (4970, CST, USA), ACE2 (Sc-20998, Santa Cruz, USA), Mas (AAR-013, Alomone labs, Israel), Fatty Acid Synthase (FAS) (3180, CST), acetyl-CoA carboxylase α (ACCα) (Sc-30212, Santa Cruz), sterol regulatory element-binding protein-1c (SREBP-1c) (Sc-366, Santa Cruz), C/EBP homologous protein (CHOP) (2895, CST), Glucose regulated protein 78 (GRP78) (ab21685, Abcam, England), activating transcription factor 4 (ATF4) (11,815, CST).

Techniques: Western Blot, Binding Assay

Journal: Microorganisms

Article Title: Levilactobacillus brevis MG5311 Alleviates Ethanol-Induced Liver Injury by Suppressing Hepatic Oxidative Stress in C57BL/6 Mice

doi: 10.3390/microorganisms10122488

Figure Lengend Snippet: Effect of L. brevis MG5311 on hepatic steatosis-related gene expression in liver tissues of ethanol-fed mice. ( A ) representative Western blot; ( B ) sterol regulatory element-binding transcription factor 1c (SREBP-1c); ( C ) sirtuin 1 (SIRT1); ( D ) phospho-AMP-activated protein kinase/AMP-activated protein kinase (p-AMPK/AMPK) ( E ) peroxisome proliferator-activated receptor α (PPARα). Data are presented as mean ± SD ( n = 3). Significance was analyzed by Dunnett’s test. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. ND group. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. EtOH group. ND, normal diet group; EtOH, ethanol diet group; MG5311, ethanol diet- L. brevis MG5311 administered group.

Article Snippet: Antibodies against aldehyde dehydrogenase 2 (ALDH2), sirtuin 1 (SIRT1), sterol regulatory element-binding protein 1c (SREBP-1c), peroxisome proliferator-activated receptor α (PPARα), and glutathione peroxidase 1/2 (GPx 1/2) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Expressing, Western Blot, Binding Assay

Journal: Microorganisms

Article Title: Levilactobacillus brevis MG5311 Alleviates Ethanol-Induced Liver Injury by Suppressing Hepatic Oxidative Stress in C57BL/6 Mice

doi: 10.3390/microorganisms10122488

Figure Lengend Snippet: L. brevis MG5311 exerts hepatoprotective effects by regulating hepatic oxidative stress and lipid metabolism. CYP2E1, Cytochrome P450 2E1; SIRT1, Sirtuin 1; SOD1, superoxide dismutase 1; GPx, glutathione peroxidase; CAT, catalase; PPARα, peroxisome proliferator-activated receptor α; SREBP-1c, sterol regulatory element-binding protein 1c.

Article Snippet: Antibodies against aldehyde dehydrogenase 2 (ALDH2), sirtuin 1 (SIRT1), sterol regulatory element-binding protein 1c (SREBP-1c), peroxisome proliferator-activated receptor α (PPARα), and glutathione peroxidase 1/2 (GPx 1/2) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Binding Assay

Journal: International Journal of Nanomedicine

Article Title: Comparing the effects of nano-sized sugarcane fiber with cellulose and psyllium on hepatic cellular signaling in mice

doi: 10.2147/IJN.S30887

Figure Lengend Snippet: Effects of dietary fibers on liver AMP-activated protein kinase (AMPK) signaling in mice. The protein abundances of AMPKα1, AMPKα2, ACC, SIRT1, sterol regulatory element-binding protein (SREBP) 1c and phosphorylated AMPKα, and ACC were measured by immunoblotting. Notes: Data are shown as mean ± standard error of the mean (n = 9/group). * P < 0.05; ** P < 0.01; *** P < 0.001, dietary fiber-supplemented group vs HFD-only group; ## P < 0.01, CEL or PSY vs SCF group. Abbreviations: CEL, cellulose; HFD, high-fat diet; PSY, psyllium; SCF, sugar cane fiber.

Article Snippet: Protein abundances were detected with antibodies against insulin receptor substrate 1 (IRS-1), insulin receptor (IR)-p (Tyr1150–1151) , IRS-1 p (Tyr612) , Akt1, Akt1 p (ser473) , AMPKα p (Thr172) , AMPKα1, AMPKα2, FGFR1, PGC-1α, SIRT1, ACC p (cer79) , ACC, and PPARα (Upstate, Lake Placid, NY), FGFR3 (Bioworld Inc, Visalia, CA), antiphosphotyrosine 20 (PY 20), insulin receptor beta (IR β), sterol regulatory element-binding protein (SREBP) 1c, and βKlotho antibodies (Santa Cruz Biotech Inc, Santa Cruz, CA), and β-actin (Affinity BioReagents Inc, Golden, CO) using Chemiluminescence Reagent Plus from PerkinElmer Life Science (Boston, MA), and quantified via a Bio-Rad universal hood II densitometer with Quantity One software (v 4.5; Bio-Rad, Hercules, CA).

Techniques: Binding Assay, Western Blot

Journal: Mediators of Inflammation

Article Title: Nutritional Recovery with a Soybean Diet after Weaning Reduces Lipogenesis but Induces Inflammation in the Liver in Adult Rats Exposed to Protein Restriction during Intrauterine Life and Lactation

doi: 10.1155/2015/781703

Figure Lengend Snippet: Immunoblot analysis of SREBP-1c (a) and PPAR γ (b) in nuclear extracts from livers of rats fed with casein (CC and LC), soybean-flour (CS and LS), or low-protein (LL) diets after weaning. The values are expressed as the mean ± SD ( n = 4–7 rats). # Mean values were significantly different from the casein diet (two-way ANOVA). Means with different superscript minuscule letters are significantly different by one-way ANOVA followed by a least significant difference (LSD) test ( P < 0.05).

Article Snippet: The membranes were blocked with 5% skim milk in Tween-Tris-buffered saline (TTBS) (10 mmol/L Tris, 150 mmol/L NaCl, 0.5% Tween 20) overnight at 4°C. nSREBP-1c and PPAR γ were detected in the membranes after a 2 h incubation at room temperature with anti-SREBP-1c rabbit polyclonal IgG and anti-PPAR γ mouse polyclonal IgG (Santa Cruz Biotechnology; diluted 1 : 500 in TTBS containing 3% dry skimmed milk).

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Changes in the Daily Rhythm of Lipid Metabolism in the Diabetic Retina

doi: 10.1371/journal.pone.0095028

Figure Lengend Snippet: Analysis of fluorescence intensity and daily rhythmicity of BMAL1 and SREBP1C by COSOPT and R analysis in rat retina.

Article Snippet: After pretreatments, sections were blocked with 10% normal goat sera (Jackson ImmunoResearch Laboratories Inc., West Grove, PA) for 2 h at room temperature and incubated with polyclonal rabbit anti-BMAL 1 antibody (Abcam Inc., Cambridge, MA) at 1∶100 or polyclonal rabbit anti-SREBP 1C antibody (Santa Cruz Biotechnology, Delaware, CA) at 1∶50 in PBS with 1% BSA for 2 h at room temperature or overnight at 4°C.

Techniques: Fluorescence

Journal: PLoS ONE

Article Title: Changes in the Daily Rhythm of Lipid Metabolism in the Diabetic Retina

doi: 10.1371/journal.pone.0095028

Figure Lengend Snippet: Cultures of rREC isolated from control and diabetic donors were exposed to 100 PPARα , PPARγ, srebp 1c, Elovl2 and Elovl4 . Expression values are normalized to cyclophilin. Values are shown as the mean ± SE, n = 3 for observations in rREC from control and diabetic rats. Statistical analysis was performed using two-way ANOVA for diabetes and time effect, and Dunnett’s post-test to compare replicates by time point, *p<0.05, **p<0.01,***p<0.001, ns means not significant.

Article Snippet: After pretreatments, sections were blocked with 10% normal goat sera (Jackson ImmunoResearch Laboratories Inc., West Grove, PA) for 2 h at room temperature and incubated with polyclonal rabbit anti-BMAL 1 antibody (Abcam Inc., Cambridge, MA) at 1∶100 or polyclonal rabbit anti-SREBP 1C antibody (Santa Cruz Biotechnology, Delaware, CA) at 1∶50 in PBS with 1% BSA for 2 h at room temperature or overnight at 4°C.

Techniques: Isolation, Expressing