mouse srebp 1c  (Biozol Diagnostica Vertrieb GmbH)

Journal: Molecules

Article Title: Influence of Genistein on Hepatic Lipid Metabolism in an In Vitro Model of Hepatic Steatosis

doi: 10.3390/molecules26041156

Figure Lengend Snippet: Adaption of gene expression of key players of lipid homeostasis in steatotic PHHs. PHHs were treated with 1 mM FFAs for 24 h and relative mRNA expression levels of gene targets were determined using RT-qPCR. Investigation of genes centrally involved in lipid catabolism (PPARα, ACSL1 and CPT1L) and anabolism (SREBP-1c and FASN) in PHHs compared to control. Data are shown as the mean + SD, n = 5, paired t-test, statistical analyses were conducted on ΔC T values, p ≤ 0.05 (*), p < 0.01 (**).

Article Snippet: Mouse SREBP-1c (Biozol, Eching, Germany) , 1:200 , 2 h.

Techniques: Expressing, Quantitative RT-PCR

Journal: Molecules

Article Title: Influence of Genistein on Hepatic Lipid Metabolism in an In Vitro Model of Hepatic Steatosis

doi: 10.3390/molecules26041156

Figure Lengend Snippet: Activation of key signaling pathways in lipid homeostasis in steatotic hepatocytes. PHHs were treated with 1 mM FFAs for 24 h and protein levels of PPARα and SREBP-1c were investigated. Western blot analyses were performed after protein extraction from different subcellular fractions of FFA-treated PHHs and control. Densitometric measurements of ( A ) cytosolic PPARα normalized to the expression levels of α-tubulin, nucleic PPARα normalized to p84, ( B ) ER membrane-bound SREBP-1c normalized to Na + /K + -ATPase and nucleic SREBP-1c normalized to p84 expression. Data are shown as the mean + SD, n = 5, paired t-test, p ≤ 0.05 (*). ( C ) Representative Western blot images show the specific bands of PPARα, α-tubulin, p84, SREBP-1c, and Na + /K + -ATPase in control and steatotic PHHs.

Article Snippet: Mouse SREBP-1c (Biozol, Eching, Germany) , 1:200 , 2 h.

Techniques: Activation Assay, Western Blot, Protein Extraction, Expressing

Journal: Molecules

Article Title: Influence of Genistein on Hepatic Lipid Metabolism in an In Vitro Model of Hepatic Steatosis

doi: 10.3390/molecules26041156

Figure Lengend Snippet: Transcriptional and translational response of genistein-treated steatotic PHHs on ( A ) PPARα and ( B ) SREBP-1c, two central regulators of hepatic lipid homeostasis. PHHs were treated with 1 mM FFAs for 24 h, followed by 24 h of additive treatment with genistein (0, 1, 5, 10, 50, and 100 µM). ( Ai ) PPARα mRNA levels were determined by RT-qPCR; ( Aii ) cytosolic and; ( Aiii ) nucleic PPARα protein levels were assessed by Western blot (α-tubulin and p84 served as the respective reference proteins). ( Bi ) Relative mRNA expression levels of SREBP-1c as measured by RT-qPCR; ( Bii ) densitometric measurements of ER membrane-bound SREBP-1c normalized to Na + /K + -ATPase and ( Biii ) nucleic SREBP-1c normalized to p84 expression. Data are shown as the mean + SD, n = 5 for RT-qPCR data, n = 4 for Western blot measurements, two-way ANOVA with Tukey or Sidak post hoc test. p ≤ 0.05 (*), p < 0.01 (**). Selected comparisons are shown; for details on the statistical evaluation, see . ( C ) Representative Western blot images show the specific bands of PPARα, α-tubulin, p84, SREBP-1c, and Na + /K + -ATPase in control and steatotic PHHs after genistein treatment.

Article Snippet: Mouse SREBP-1c (Biozol, Eching, Germany) , 1:200 , 2 h.

Techniques: Quantitative RT-PCR, Western Blot, Expressing

Journal: Molecules

Article Title: Influence of Genistein on Hepatic Lipid Metabolism in an In Vitro Model of Hepatic Steatosis

doi: 10.3390/molecules26041156

Figure Lengend Snippet: TaqMan ® gene expression assays (Applied Biosystems, Foster City, CA, USA) applied for qPCR.

Article Snippet: Mouse SREBP-1c (Biozol, Eching, Germany) , 1:200 , 2 h.

Techniques: Expressing, Binding Assay

Journal: Molecules

Article Title: Influence of Genistein on Hepatic Lipid Metabolism in an In Vitro Model of Hepatic Steatosis

doi: 10.3390/molecules26041156

Figure Lengend Snippet: Antibodies for Western blotting.

Article Snippet: Mouse SREBP-1c (Biozol, Eching, Germany) , 1:200 , 2 h.

Techniques: Western Blot, Incubation