Structured Review

Proteintech anti phd2
Effects of constant light exposure on renal expression of HIF1α, NOX4 and PHD. (A, B) qPCR analysis of mRNA levels of HIF1α and NOX4 in renal tissues of rats. (C) IHC staining of renal HIF1α and semi-quantification analysis. (D) Western blot assays of HIF1α expression in renal tissues. (E) Western blot assays of PHD1 and <t>PHD2</t> expression in the renal tissues. (F) qPCR analysis of mRNA levels of PHD1 (Egln2) , PHD2 (Egln1) , and PHD3 (Egln3) in the kidney. Values represent mean ± SEM (n=6 for A, B and F , n=8 for C ). Differences were determined using a two-way ANOVA followed by a Bonferroni post hoc analysis. # p <0.05, ## p <0.01, ### p <0.001, vs. ND counterpart. * p <0.05, ** p <0.01, vs. LD counterpart. p (diet), main effect of diet; p (light), main effect of light; p (light × diet), interaction effect of light and diet.
Anti Phd2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Chronic constant light exposure aggravates high fat diet-induced renal injury in rats"

Article Title: Chronic constant light exposure aggravates high fat diet-induced renal injury in rats

Journal: Frontiers in Endocrinology

doi: 10.3389/fendo.2022.900392

Effects of constant light exposure on renal expression of HIF1α, NOX4 and PHD. (A, B) qPCR analysis of mRNA levels of HIF1α and NOX4 in renal tissues of rats. (C) IHC staining of renal HIF1α and semi-quantification analysis. (D) Western blot assays of HIF1α expression in renal tissues. (E) Western blot assays of PHD1 and PHD2 expression in the renal tissues. (F) qPCR analysis of mRNA levels of PHD1 (Egln2) , PHD2 (Egln1) , and PHD3 (Egln3) in the kidney. Values represent mean ± SEM (n=6 for A, B and F , n=8 for C ). Differences were determined using a two-way ANOVA followed by a Bonferroni post hoc analysis. # p <0.05, ## p <0.01, ### p <0.001, vs. ND counterpart. * p <0.05, ** p <0.01, vs. LD counterpart. p (diet), main effect of diet; p (light), main effect of light; p (light × diet), interaction effect of light and diet.
Figure Legend Snippet: Effects of constant light exposure on renal expression of HIF1α, NOX4 and PHD. (A, B) qPCR analysis of mRNA levels of HIF1α and NOX4 in renal tissues of rats. (C) IHC staining of renal HIF1α and semi-quantification analysis. (D) Western blot assays of HIF1α expression in renal tissues. (E) Western blot assays of PHD1 and PHD2 expression in the renal tissues. (F) qPCR analysis of mRNA levels of PHD1 (Egln2) , PHD2 (Egln1) , and PHD3 (Egln3) in the kidney. Values represent mean ± SEM (n=6 for A, B and F , n=8 for C ). Differences were determined using a two-way ANOVA followed by a Bonferroni post hoc analysis. # p <0.05, ## p <0.01, ### p <0.001, vs. ND counterpart. * p <0.05, ** p <0.01, vs. LD counterpart. p (diet), main effect of diet; p (light), main effect of light; p (light × diet), interaction effect of light and diet.

Techniques Used: Expressing, Immunohistochemistry, Western Blot

ChIP-qPCR assays of BMAL1/CLOCK on HIF1α and Egln1/2 promoters in podocytes (MPC5). (A) Human HIF1α and mouse Hif1α promoter both contain one E-box. (B) Human PHD2 (gene EGLN1 ) and mouse PHD2 (gene Egln1 ) promoter both contain one E-box. (C) Human PHD1 (gene EGLN2 ) and mouse PHD1 (gene Egln2 ) promoter both contain three E-boxes. (D) Enrichment of BMAL1 was evaluated by quantitative PCR of immunoprecipitated DNA compared with input DNA (% of input). Immunoglobulin G (IgG) was used as a negative control. Values represent mean ± SEM. Differences were determined using two-tailed Student t-test. **p<0.01.
Figure Legend Snippet: ChIP-qPCR assays of BMAL1/CLOCK on HIF1α and Egln1/2 promoters in podocytes (MPC5). (A) Human HIF1α and mouse Hif1α promoter both contain one E-box. (B) Human PHD2 (gene EGLN1 ) and mouse PHD2 (gene Egln1 ) promoter both contain one E-box. (C) Human PHD1 (gene EGLN2 ) and mouse PHD1 (gene Egln2 ) promoter both contain three E-boxes. (D) Enrichment of BMAL1 was evaluated by quantitative PCR of immunoprecipitated DNA compared with input DNA (% of input). Immunoglobulin G (IgG) was used as a negative control. Values represent mean ± SEM. Differences were determined using two-tailed Student t-test. **p<0.01.

Techniques Used: Real-time Polymerase Chain Reaction, Immunoprecipitation, Negative Control, Two Tailed Test


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Proteintech phd2
Phd2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech anti phd2
HIF2α-mediated GO terms involved in the development of <t>Phd2</t> -KO mediated spontaneous PH. (A) A representative waveform shows RVP (left) and statistical data (right) represents peak RVSP in WT, Phd2 EC +/– and Phd2 EC – /– mice ( n = 6). Scale bars = 0.2 sec. (B) Statistical data of the RV/(LV + S) ratio shows RV hypertrophy defined by the Fulton index as a ratio [RV/(LV + S)] in WT, Phd2 EC +/– and Phd2 EC – /– mice ( n = 6). (C) Typical H & E images of pulmonary arterioles from WT, Phd2 EC +/– and Phd2 EC – /– mice. Scale bars = 10 μm. (D) Statistical data shows PA wall thickness that generated by the ratio of wall area to total vessel area in PAs which were restricted less than 100 μm in diameter from WT, Phd2 EC +/– or Phd2 EC – /– mice ( n = 6). (E) Expression heatmap of the genes commonly deregulated in both hypoxia and Phd2 EC – /– deficient groups. Red and blue represent relative up-regulation and down-regulation of gene expression, respectively. (F) Correlation in log 2 FC between hypoxia vs. normoxia mice ( X -axis) and Phd2 EC – /– vs. WT mice ( Y -axis) The correlation coefficient (r) and P -value were computed by Pearson correlation test. (G) Gene set score heatmap of the HIF2α-mediated GO biological process terms in WT and Phd2 EC – /– mice. In total, seven GO terms up-regulated in Phd2 EC – /– lungs were listed. Red and blue represent relative up-regulation and down-regulation of gene expression, respectively. (H) Comparison of gene set score of the TGF-β signaling pathway between WT and Phd2 EC – /– mice. (I) Genotyping of WT and Phd2 EC +/– mice. PC1: positive control 1 ( Phd2 f /+ ), PC2: positive control 2 (Tie2-Cre +/– ), NC1: negative control 1 ( Phd2 +/+ ), NC2: negative control 2 (Tie2-Cre – /– ). (J) Relative expression of the PHD2 and HIF2α in mice lung tissues detected by western blotting with β-actin as control ( n = 3). Significance levels: * P < 0.05, ** P < 0.01 and *** P < 0.001 ( t -test).
Anti Phd2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Hypoxia-Inducible Factor 2-Alpha Mediated Gene Sets Differentiate Pulmonary Arterial Hypertension"

Article Title: Hypoxia-Inducible Factor 2-Alpha Mediated Gene Sets Differentiate Pulmonary Arterial Hypertension

Journal: Frontiers in Cell and Developmental Biology

doi: 10.3389/fcell.2021.701247

HIF2α-mediated GO terms involved in the development of Phd2 -KO mediated spontaneous PH. (A) A representative waveform shows RVP (left) and statistical data (right) represents peak RVSP in WT, Phd2 EC +/– and Phd2 EC – /– mice ( n = 6). Scale bars = 0.2 sec. (B) Statistical data of the RV/(LV + S) ratio shows RV hypertrophy defined by the Fulton index as a ratio [RV/(LV + S)] in WT, Phd2 EC +/– and Phd2 EC – /– mice ( n = 6). (C) Typical H & E images of pulmonary arterioles from WT, Phd2 EC +/– and Phd2 EC – /– mice. Scale bars = 10 μm. (D) Statistical data shows PA wall thickness that generated by the ratio of wall area to total vessel area in PAs which were restricted less than 100 μm in diameter from WT, Phd2 EC +/– or Phd2 EC – /– mice ( n = 6). (E) Expression heatmap of the genes commonly deregulated in both hypoxia and Phd2 EC – /– deficient groups. Red and blue represent relative up-regulation and down-regulation of gene expression, respectively. (F) Correlation in log 2 FC between hypoxia vs. normoxia mice ( X -axis) and Phd2 EC – /– vs. WT mice ( Y -axis) The correlation coefficient (r) and P -value were computed by Pearson correlation test. (G) Gene set score heatmap of the HIF2α-mediated GO biological process terms in WT and Phd2 EC – /– mice. In total, seven GO terms up-regulated in Phd2 EC – /– lungs were listed. Red and blue represent relative up-regulation and down-regulation of gene expression, respectively. (H) Comparison of gene set score of the TGF-β signaling pathway between WT and Phd2 EC – /– mice. (I) Genotyping of WT and Phd2 EC +/– mice. PC1: positive control 1 ( Phd2 f /+ ), PC2: positive control 2 (Tie2-Cre +/– ), NC1: negative control 1 ( Phd2 +/+ ), NC2: negative control 2 (Tie2-Cre – /– ). (J) Relative expression of the PHD2 and HIF2α in mice lung tissues detected by western blotting with β-actin as control ( n = 3). Significance levels: * P < 0.05, ** P < 0.01 and *** P < 0.001 ( t -test).
Figure Legend Snippet: HIF2α-mediated GO terms involved in the development of Phd2 -KO mediated spontaneous PH. (A) A representative waveform shows RVP (left) and statistical data (right) represents peak RVSP in WT, Phd2 EC +/– and Phd2 EC – /– mice ( n = 6). Scale bars = 0.2 sec. (B) Statistical data of the RV/(LV + S) ratio shows RV hypertrophy defined by the Fulton index as a ratio [RV/(LV + S)] in WT, Phd2 EC +/– and Phd2 EC – /– mice ( n = 6). (C) Typical H & E images of pulmonary arterioles from WT, Phd2 EC +/– and Phd2 EC – /– mice. Scale bars = 10 μm. (D) Statistical data shows PA wall thickness that generated by the ratio of wall area to total vessel area in PAs which were restricted less than 100 μm in diameter from WT, Phd2 EC +/– or Phd2 EC – /– mice ( n = 6). (E) Expression heatmap of the genes commonly deregulated in both hypoxia and Phd2 EC – /– deficient groups. Red and blue represent relative up-regulation and down-regulation of gene expression, respectively. (F) Correlation in log 2 FC between hypoxia vs. normoxia mice ( X -axis) and Phd2 EC – /– vs. WT mice ( Y -axis) The correlation coefficient (r) and P -value were computed by Pearson correlation test. (G) Gene set score heatmap of the HIF2α-mediated GO biological process terms in WT and Phd2 EC – /– mice. In total, seven GO terms up-regulated in Phd2 EC – /– lungs were listed. Red and blue represent relative up-regulation and down-regulation of gene expression, respectively. (H) Comparison of gene set score of the TGF-β signaling pathway between WT and Phd2 EC – /– mice. (I) Genotyping of WT and Phd2 EC +/– mice. PC1: positive control 1 ( Phd2 f /+ ), PC2: positive control 2 (Tie2-Cre +/– ), NC1: negative control 1 ( Phd2 +/+ ), NC2: negative control 2 (Tie2-Cre – /– ). (J) Relative expression of the PHD2 and HIF2α in mice lung tissues detected by western blotting with β-actin as control ( n = 3). Significance levels: * P < 0.05, ** P < 0.01 and *** P < 0.001 ( t -test).

Techniques Used: Generated, Expressing, Positive Control, Negative Control, Western Blot


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Proteintech phd2
( a – c ) EGLN1 ( a ), hypoxia inducible factor (HIF) -1α ( b ), and VEGF ( c ) mRNA levels in MH7A cells were analyzed using quantitative reverse-transcription polymerase chain reaction (RT–PCR) after 72 h of hypoxia (1% O 2 ). Each value represents the mean ± SD. (n = 6); * p < 0.05. ( d – f ) Prolyl hydroxylase domain-containing protein 2 <t>(PHD2)</t> and HIF-1α protein expression was analyzed using Western blotting; β-actin was used as the loading control.
Phd2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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1) Product Images from "Sustained Hypoxia Suppresses Joint Destruction in a Rat Model of Rheumatoid Arthritis via Negative Feedback of Hypoxia Inducible Factor-1α"

Article Title: Sustained Hypoxia Suppresses Joint Destruction in a Rat Model of Rheumatoid Arthritis via Negative Feedback of Hypoxia Inducible Factor-1α

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms22083898

( a – c ) EGLN1 ( a ), hypoxia inducible factor (HIF) -1α ( b ), and VEGF ( c ) mRNA levels in MH7A cells were analyzed using quantitative reverse-transcription polymerase chain reaction (RT–PCR) after 72 h of hypoxia (1% O 2 ). Each value represents the mean ± SD. (n = 6); * p < 0.05. ( d – f ) Prolyl hydroxylase domain-containing protein 2 (PHD2) and HIF-1α protein expression was analyzed using Western blotting; β-actin was used as the loading control.
Figure Legend Snippet: ( a – c ) EGLN1 ( a ), hypoxia inducible factor (HIF) -1α ( b ), and VEGF ( c ) mRNA levels in MH7A cells were analyzed using quantitative reverse-transcription polymerase chain reaction (RT–PCR) after 72 h of hypoxia (1% O 2 ). Each value represents the mean ± SD. (n = 6); * p < 0.05. ( d – f ) Prolyl hydroxylase domain-containing protein 2 (PHD2) and HIF-1α protein expression was analyzed using Western blotting; β-actin was used as the loading control.

Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot


Structured Review

Proteintech anti phd2
Anti Phd2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti phd2/product/Proteintech
Average 93 stars, based on 1 article reviews
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Proteintech 19886 1 ap
19886 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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19886 1 ap - by Bioz Stars, 2024-04
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Proteintech phd2
Antibodies used in the present study.
Phd2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phd2/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
phd2 - by Bioz Stars, 2024-04
93/100 stars

Images

1) Product Images from "Antitumor effects of aconitine in A2780 cells via estrogen receptor β-mediated apoptosis, DNA damage and migration"

Article Title: Antitumor effects of aconitine in A2780 cells via estrogen receptor β-mediated apoptosis, DNA damage and migration

Journal: Molecular Medicine Reports

doi: 10.3892/mmr.2020.11322

Antibodies used in the present study.
Figure Legend Snippet: Antibodies used in the present study.

Techniques Used:

Effects of aconitine on the expression of proteins associated with apoptosis, DNA damage and migration. (A) Effects of aconitine on the expression levels of HIF-α and PHD2. (B) Effects of aconitine on the expression levels of MMP2 and MMP9. (C) Effects of aconitine on the expression levels of ATM, p-ATM and p53. Data are presented as mean ± SD of three independent experiments. *P<0.05, **P<0.01 vs. control group. Aco, aconitine; HIF-α, hypoxia-inducible factor; PHD2, prolyl hydroxylase domain-containing protein 2; MMP, matrix metalloproteinase; ATM, ATM serine/threonine kinase; p-, phosphorylated.
Figure Legend Snippet: Effects of aconitine on the expression of proteins associated with apoptosis, DNA damage and migration. (A) Effects of aconitine on the expression levels of HIF-α and PHD2. (B) Effects of aconitine on the expression levels of MMP2 and MMP9. (C) Effects of aconitine on the expression levels of ATM, p-ATM and p53. Data are presented as mean ± SD of three independent experiments. *P<0.05, **P<0.01 vs. control group. Aco, aconitine; HIF-α, hypoxia-inducible factor; PHD2, prolyl hydroxylase domain-containing protein 2; MMP, matrix metalloproteinase; ATM, ATM serine/threonine kinase; p-, phosphorylated.

Techniques Used: Expressing, Migration


Structured Review

Proteintech phd2
Expression of the various components of the CRL2 VHL complex in the presence or absence of MUL1 protein. (A) The differential expression of CUL2, VHL, <t>PHD2,</t> Elongin B, Elongin C and RBX1 proteins in HEK293 MUL1(+/+) and HEK293 MUL1(−/−) cells was monitored by SDS-PAGE and Western blot analysis. β-actin antibody was used to verify equal loading in each lane. (B) Graph summarizing the differential modulation of the various components of the CRL2 VHL complex between the HEK293 MUL1(+/+) and HEK293 MUL1(−/−) cells using densitometric analysis of the protein expression data from Figs. and 4A. The scale of the graph is presented as log 5 . (C) HEK293 MUL1(+/+) and HEK293 MUL1(−/−) cells were treated with the neddylation inhibitor MLN4924 (1 μM) for various time point (2, 4 and 8 hours). The expression of UBXN7, HIF-1α, CUL2 as well as neddylated-CUL2 was monitored. (D) Graph represents densitometric analysis of the UBXN7 and HIF-1α protein expression from ( C ) normalized against β-actin. * p < 0.03 vs HEK293 MUL1(+/+) control and # p < 0.001 vs HEK293 MUL1(+/+). Results shown are means ± S.D. of three independent experiments.
Phd2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Mitochondrial MUL1 E3 ubiquitin ligase regulates Hypoxia Inducible Factor (HIF-1α) and metabolic reprogramming by modulating the UBXN7 cofactor protein"

Article Title: Mitochondrial MUL1 E3 ubiquitin ligase regulates Hypoxia Inducible Factor (HIF-1α) and metabolic reprogramming by modulating the UBXN7 cofactor protein

Journal: Scientific Reports

doi: 10.1038/s41598-020-58484-8

Expression of the various components of the CRL2 VHL complex in the presence or absence of MUL1 protein. (A) The differential expression of CUL2, VHL, PHD2, Elongin B, Elongin C and RBX1 proteins in HEK293 MUL1(+/+) and HEK293 MUL1(−/−) cells was monitored by SDS-PAGE and Western blot analysis. β-actin antibody was used to verify equal loading in each lane. (B) Graph summarizing the differential modulation of the various components of the CRL2 VHL complex between the HEK293 MUL1(+/+) and HEK293 MUL1(−/−) cells using densitometric analysis of the protein expression data from Figs. and 4A. The scale of the graph is presented as log 5 . (C) HEK293 MUL1(+/+) and HEK293 MUL1(−/−) cells were treated with the neddylation inhibitor MLN4924 (1 μM) for various time point (2, 4 and 8 hours). The expression of UBXN7, HIF-1α, CUL2 as well as neddylated-CUL2 was monitored. (D) Graph represents densitometric analysis of the UBXN7 and HIF-1α protein expression from ( C ) normalized against β-actin. * p < 0.03 vs HEK293 MUL1(+/+) control and # p < 0.001 vs HEK293 MUL1(+/+). Results shown are means ± S.D. of three independent experiments.
Figure Legend Snippet: Expression of the various components of the CRL2 VHL complex in the presence or absence of MUL1 protein. (A) The differential expression of CUL2, VHL, PHD2, Elongin B, Elongin C and RBX1 proteins in HEK293 MUL1(+/+) and HEK293 MUL1(−/−) cells was monitored by SDS-PAGE and Western blot analysis. β-actin antibody was used to verify equal loading in each lane. (B) Graph summarizing the differential modulation of the various components of the CRL2 VHL complex between the HEK293 MUL1(+/+) and HEK293 MUL1(−/−) cells using densitometric analysis of the protein expression data from Figs. and 4A. The scale of the graph is presented as log 5 . (C) HEK293 MUL1(+/+) and HEK293 MUL1(−/−) cells were treated with the neddylation inhibitor MLN4924 (1 μM) for various time point (2, 4 and 8 hours). The expression of UBXN7, HIF-1α, CUL2 as well as neddylated-CUL2 was monitored. (D) Graph represents densitometric analysis of the UBXN7 and HIF-1α protein expression from ( C ) normalized against β-actin. * p < 0.03 vs HEK293 MUL1(+/+) control and # p < 0.001 vs HEK293 MUL1(+/+). Results shown are means ± S.D. of three independent experiments.

Techniques Used: Expressing, SDS Page, Western Blot

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    Proteintech anti phd2
    Effects of constant light exposure on renal expression of HIF1α, NOX4 and PHD. (A, B) qPCR analysis of mRNA levels of HIF1α and NOX4 in renal tissues of rats. (C) IHC staining of renal HIF1α and semi-quantification analysis. (D) Western blot assays of HIF1α expression in renal tissues. (E) Western blot assays of PHD1 and <t>PHD2</t> expression in the renal tissues. (F) qPCR analysis of mRNA levels of PHD1 (Egln2) , PHD2 (Egln1) , and PHD3 (Egln3) in the kidney. Values represent mean ± SEM (n=6 for A, B and F , n=8 for C ). Differences were determined using a two-way ANOVA followed by a Bonferroni post hoc analysis. # p <0.05, ## p <0.01, ### p <0.001, vs. ND counterpart. * p <0.05, ** p <0.01, vs. LD counterpart. p (diet), main effect of diet; p (light), main effect of light; p (light × diet), interaction effect of light and diet.
    Anti Phd2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phd2/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phd2 - by Bioz Stars, 2024-04
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    93
    Proteintech phd2
    Effects of constant light exposure on renal expression of HIF1α, NOX4 and PHD. (A, B) qPCR analysis of mRNA levels of HIF1α and NOX4 in renal tissues of rats. (C) IHC staining of renal HIF1α and semi-quantification analysis. (D) Western blot assays of HIF1α expression in renal tissues. (E) Western blot assays of PHD1 and <t>PHD2</t> expression in the renal tissues. (F) qPCR analysis of mRNA levels of PHD1 (Egln2) , PHD2 (Egln1) , and PHD3 (Egln3) in the kidney. Values represent mean ± SEM (n=6 for A, B and F , n=8 for C ). Differences were determined using a two-way ANOVA followed by a Bonferroni post hoc analysis. # p <0.05, ## p <0.01, ### p <0.001, vs. ND counterpart. * p <0.05, ** p <0.01, vs. LD counterpart. p (diet), main effect of diet; p (light), main effect of light; p (light × diet), interaction effect of light and diet.
    Phd2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phd2/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phd2 - by Bioz Stars, 2024-04
    93/100 stars
      Buy from Supplier

    93
    Proteintech 19886 1 ap
    Effects of constant light exposure on renal expression of HIF1α, NOX4 and PHD. (A, B) qPCR analysis of mRNA levels of HIF1α and NOX4 in renal tissues of rats. (C) IHC staining of renal HIF1α and semi-quantification analysis. (D) Western blot assays of HIF1α expression in renal tissues. (E) Western blot assays of PHD1 and <t>PHD2</t> expression in the renal tissues. (F) qPCR analysis of mRNA levels of PHD1 (Egln2) , PHD2 (Egln1) , and PHD3 (Egln3) in the kidney. Values represent mean ± SEM (n=6 for A, B and F , n=8 for C ). Differences were determined using a two-way ANOVA followed by a Bonferroni post hoc analysis. # p <0.05, ## p <0.01, ### p <0.001, vs. ND counterpart. * p <0.05, ** p <0.01, vs. LD counterpart. p (diet), main effect of diet; p (light), main effect of light; p (light × diet), interaction effect of light and diet.
    19886 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/19886 1 ap/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    19886 1 ap - by Bioz Stars, 2024-04
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    Effects of constant light exposure on renal expression of HIF1α, NOX4 and PHD. (A, B) qPCR analysis of mRNA levels of HIF1α and NOX4 in renal tissues of rats. (C) IHC staining of renal HIF1α and semi-quantification analysis. (D) Western blot assays of HIF1α expression in renal tissues. (E) Western blot assays of PHD1 and PHD2 expression in the renal tissues. (F) qPCR analysis of mRNA levels of PHD1 (Egln2) , PHD2 (Egln1) , and PHD3 (Egln3) in the kidney. Values represent mean ± SEM (n=6 for A, B and F , n=8 for C ). Differences were determined using a two-way ANOVA followed by a Bonferroni post hoc analysis. # p <0.05, ## p <0.01, ### p <0.001, vs. ND counterpart. * p <0.05, ** p <0.01, vs. LD counterpart. p (diet), main effect of diet; p (light), main effect of light; p (light × diet), interaction effect of light and diet.

    Journal: Frontiers in Endocrinology

    Article Title: Chronic constant light exposure aggravates high fat diet-induced renal injury in rats

    doi: 10.3389/fendo.2022.900392

    Figure Lengend Snippet: Effects of constant light exposure on renal expression of HIF1α, NOX4 and PHD. (A, B) qPCR analysis of mRNA levels of HIF1α and NOX4 in renal tissues of rats. (C) IHC staining of renal HIF1α and semi-quantification analysis. (D) Western blot assays of HIF1α expression in renal tissues. (E) Western blot assays of PHD1 and PHD2 expression in the renal tissues. (F) qPCR analysis of mRNA levels of PHD1 (Egln2) , PHD2 (Egln1) , and PHD3 (Egln3) in the kidney. Values represent mean ± SEM (n=6 for A, B and F , n=8 for C ). Differences were determined using a two-way ANOVA followed by a Bonferroni post hoc analysis. # p <0.05, ## p <0.01, ### p <0.001, vs. ND counterpart. * p <0.05, ** p <0.01, vs. LD counterpart. p (diet), main effect of diet; p (light), main effect of light; p (light × diet), interaction effect of light and diet.

    Article Snippet: The membranes were incubated with anti-TGF-β (1:1000 dilution, Abcam, Cambridge, UK), anti-HIF1α (1 μg/mL, R&D systems, Minneapolis, MN, USA), anti-PHD1 (1:1000 dilution, Proteintech, Wuhan, China), anti-PHD2 (1:500 dilution, Proteintech, Wuhan, China), and anti-β-actin (1:5000 dilution, Proteintech, Wuhan, China) at 4°C overnight and then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Proteintech, Wuhan, China) at room temperature for 90 min.

    Techniques: Expressing, Immunohistochemistry, Western Blot

    ChIP-qPCR assays of BMAL1/CLOCK on HIF1α and Egln1/2 promoters in podocytes (MPC5). (A) Human HIF1α and mouse Hif1α promoter both contain one E-box. (B) Human PHD2 (gene EGLN1 ) and mouse PHD2 (gene Egln1 ) promoter both contain one E-box. (C) Human PHD1 (gene EGLN2 ) and mouse PHD1 (gene Egln2 ) promoter both contain three E-boxes. (D) Enrichment of BMAL1 was evaluated by quantitative PCR of immunoprecipitated DNA compared with input DNA (% of input). Immunoglobulin G (IgG) was used as a negative control. Values represent mean ± SEM. Differences were determined using two-tailed Student t-test. **p<0.01.

    Journal: Frontiers in Endocrinology

    Article Title: Chronic constant light exposure aggravates high fat diet-induced renal injury in rats

    doi: 10.3389/fendo.2022.900392

    Figure Lengend Snippet: ChIP-qPCR assays of BMAL1/CLOCK on HIF1α and Egln1/2 promoters in podocytes (MPC5). (A) Human HIF1α and mouse Hif1α promoter both contain one E-box. (B) Human PHD2 (gene EGLN1 ) and mouse PHD2 (gene Egln1 ) promoter both contain one E-box. (C) Human PHD1 (gene EGLN2 ) and mouse PHD1 (gene Egln2 ) promoter both contain three E-boxes. (D) Enrichment of BMAL1 was evaluated by quantitative PCR of immunoprecipitated DNA compared with input DNA (% of input). Immunoglobulin G (IgG) was used as a negative control. Values represent mean ± SEM. Differences were determined using two-tailed Student t-test. **p<0.01.

    Article Snippet: The membranes were incubated with anti-TGF-β (1:1000 dilution, Abcam, Cambridge, UK), anti-HIF1α (1 μg/mL, R&D systems, Minneapolis, MN, USA), anti-PHD1 (1:1000 dilution, Proteintech, Wuhan, China), anti-PHD2 (1:500 dilution, Proteintech, Wuhan, China), and anti-β-actin (1:5000 dilution, Proteintech, Wuhan, China) at 4°C overnight and then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Proteintech, Wuhan, China) at room temperature for 90 min.

    Techniques: Real-time Polymerase Chain Reaction, Immunoprecipitation, Negative Control, Two Tailed Test