e coli k12  (ATCC)

Journal: bioRxiv

Article Title: Evaluating the Survival and Removal of Escherichia coli from Surfaces Made with Traditional and Sustainable Cement-Based Materials in Field-Relevant Conditions

doi: 10.1101/2024.11.07.622504

Figure Lengend Snippet: Natural log reduction of E. coli versus time (hours). The data are split into subplots based on the presence of soil and the environmental conditions for the incubation of the cement-based tile. The average reductions in concentration are plotted for each trial and the three mix designs,with error bars representing the 95% confidence interval for the reduction based on the CFU counts within each trial. Data points are jittered to better visualize the differences between the mix designs.

Article Snippet: E. coli K12 (ATCC 19853) cultures were prepared by inoculating 20 mL of tryptic soy broth (TSB; Becton, Dickinson, and Company, Sparks, MD, USA) with a loop of bacterial stock and incubating the culture at 37°C overnight.

Techniques: Incubation, Concentration Assay

Journal: bioRxiv

Article Title: Evaluating the Survival and Removal of Escherichia coli from Surfaces Made with Traditional and Sustainable Cement-Based Materials in Field-Relevant Conditions

doi: 10.1101/2024.11.07.622504

Figure Lengend Snippet: Percent removal of E. coli from cement given three removal activities. The data are split into subplots based on the presence of soil. The average percent removal for each of the three trials (opaque points) and the percent removal from each trial (transparent points) are plotted, in addition to error bars representing the 95% confidence interval for the trials. Mix designs are ordered in decreasing average roughness measurements.

Article Snippet: E. coli K12 (ATCC 19853) cultures were prepared by inoculating 20 mL of tryptic soy broth (TSB; Becton, Dickinson, and Company, Sparks, MD, USA) with a loop of bacterial stock and incubating the culture at 37°C overnight.

Techniques:

Journal: F1000Research

Article Title: A guide to selecting high-performing antibodies for CSNK2A1 (UniProt ID: P68400) for use in western blot, immunoprecipitation and immunofluorescence

doi: 10.12688/f1000research.153243.2

Figure Lengend Snippet: Summary of the CSNK2A1 antibodies tested.

Article Snippet: Bio-Techne , NBP3-19853 , 230458 , AB_3073949 , recombinant-mono , S05-7F8 , rabbit , 0.3 , Wb.

Techniques: Concentration Assay, Recombinant

Journal: F1000Research

Article Title: A guide to selecting high-performing antibodies for CSNK2A1 (UniProt ID: P68400) for use in western blot, immunoprecipitation and immunofluorescence

doi: 10.12688/f1000research.153243.2

Figure Lengend Snippet: Lysates of HAP1 (WT and CSNK2A KO) were prepared and 30 μg of protein were processed for western blot with the indicated CSNK2A1 antibodies. The ponceau stained transfers of each blot are presented to show equal loading of WT and KO lysates and protein transfer efficiency from the polyacrylamide gels to the nitrocellulose membrane. Antibody dilutions were chosen according to the recommendations of the antibody supplier. An exception was given to 68200-1-Ig* recommended at 1/20 000, as the signal was too weak and was therefore diluted and was used at 1/10 000. Antibody dilution used: ab76040** at 1/500, ab236664 at 1/1000, MAB7957* at 1/1000, NBP3-19853** at 1/1000, 2656 at 1/500, GTX107576 at 1/500, GTX107897 at 1/500, GTX107949 at 1/500, 68200-1-Ig* at 1/10 000, 702811** at 1/10 000. Predicted band size: 45 kDa. *Monoclonal antibody, **Recombinant antibody.

Article Snippet: Bio-Techne , NBP3-19853 , 230458 , AB_3073949 , recombinant-mono , S05-7F8 , rabbit , 0.3 , Wb.

Techniques: Western Blot, Staining, Membrane, Recombinant

Journal: F1000Research

Article Title: A guide to selecting high-performing antibodies for CSNK2A1 (UniProt ID: P68400) for use in western blot, immunoprecipitation and immunofluorescence

doi: 10.12688/f1000research.153243.2

Figure Lengend Snippet: HAP1 WT and CSNK2A1 KO cells were labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat-bottom. Cells were stained with the indicated CSNK2A1 antibodies and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the merged blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. When the concentration was not indicated by the supplier, antibodies were tested at concentrations where the signal from each antibody was in the range of detection of the microscope used. Antibody dilution used: ab76040** at 1/500, ab236664 at 1/100, MAB7957* at 1/500, NBP3-19853** at 1/300, 2656 at 1/30, GTX107576 at 1/100, GTX107897 at 1/100, GTX107949 at 1/200, 68200-1-Ig* at 1/500, 702811** at 1/250. Bars = 10 μm. *Monoclonal antibody, **Recombinant antibody.

Article Snippet: Bio-Techne , NBP3-19853 , 230458 , AB_3073949 , recombinant-mono , S05-7F8 , rabbit , 0.3 , Wb.

Techniques: Staining, Concentration Assay, Microscopy, Recombinant