Journal: BMC Medical Genomics
Article Title: m6A regulator-based molecular classification and hub genes associated with immune infiltration characteristics and clinical outcomes in diffuse gliomas
doi: 10.1186/s12920-025-02104-9
Figure Lengend Snippet: The role of FTO in regulating glioma proliferation and macrophage chemotaxis. A , Overexpression of FTO inhibited the expressions of PTX3 and SPAG4. B-D , Overexpression of FTO inhibited the proliferation of glioma cells in CCK8 (B) and Edu (C-D) assay. E-F , Overexpression of FTO reduced macrophage migration in transwell assay. The white bar represents 100 μm. *, P < 0.05; **, P < 0.01
Article Snippet: The membranes were incubated with primary antibodies at 1:1000 dilution and 4 °C overnight (anti- FTO , anti- SPAG4 , anti- PTX3 : Proteintech; anti- GDF15 : Abcam).
Techniques: Chemotaxis Assay, Over Expression, Migration, Transwell Assay