Journal: Foods

Article Title: Use of Doehlert Matrix as a Tool for High-Throughput Screening of Organic Acids and Essential Oils on Miniaturized Pork Loins, Followed by Lab-Scale Validation That Confirmed Tested Compounds Do Not Show Synergistic Effects against Salmonella Typhimurium

doi: 10.3390/foods12214034

Figure Lengend Snippet: Essential oil library screened for their individual antimicrobial activity against Salmonella Typhimurium ATCC 19585 on miniaturized pork disks.

Article Snippet: Thirty-five potential antimicrobials were screened at commercial working concentrations by individually adding them to miniaturized (69 cm 3 ) disks of pork loin ends, followed by inoculation with Salmonella Typhimurium ATCC 19585.

Techniques: Activity Assay, Single-particle Tracking

Journal: Foods

Article Title: Use of Doehlert Matrix as a Tool for High-Throughput Screening of Organic Acids and Essential Oils on Miniaturized Pork Loins, Followed by Lab-Scale Validation That Confirmed Tested Compounds Do Not Show Synergistic Effects against Salmonella Typhimurium

doi: 10.3390/foods12214034

Figure Lengend Snippet: Antimicrobial screening of chemically polar compounds that were surface-applied to miniaturized pork disks previously inoculated with Salmonella Typhimurium ATCC 19585. Results are presented as the difference in cell counts after 24 h incubation at 4 °C; experiments were performed in biological triplicate. Antimicrobial treatments tested were formic acid (FA), lactic acid (LA), peracetic acid (PA), potassium sorbate (PS), and sodium propionate (SOP). Antimicrobial treatment concentrations are represented as % ( v / w ). Statistically significant treatments that had p < 0.05 from untreated sample have specific p -values annotated, while all other compounds had p > 0.05. Statistically significant compounds were identified through a one-way ANOVA followed by Dunnett’s multiple comparisons test.

Article Snippet: Thirty-five potential antimicrobials were screened at commercial working concentrations by individually adding them to miniaturized (69 cm 3 ) disks of pork loin ends, followed by inoculation with Salmonella Typhimurium ATCC 19585.

Techniques: Incubation

Journal: Foods

Article Title: Use of Doehlert Matrix as a Tool for High-Throughput Screening of Organic Acids and Essential Oils on Miniaturized Pork Loins, Followed by Lab-Scale Validation That Confirmed Tested Compounds Do Not Show Synergistic Effects against Salmonella Typhimurium

doi: 10.3390/foods12214034

Figure Lengend Snippet: Antimicrobial screening of EO library. EOs were surface-applied to miniaturized pork disks previously inoculated with Salmonella Typhimurium ATCC 19585. Results are presented as the difference in cell counts after 24 h incubation at 4 °C. Experiments were carried out in biological duplicate. Statistically significant EOs were identified through a one-way ANOVA followed by Dunnett’s multiple comparisons test. Statistically significant treatments that had p < 0.05 from untreated sample have specific p -values annotated, while all other compounds had p > 0.05.

Article Snippet: Thirty-five potential antimicrobials were screened at commercial working concentrations by individually adding them to miniaturized (69 cm 3 ) disks of pork loin ends, followed by inoculation with Salmonella Typhimurium ATCC 19585.

Techniques: Incubation

Journal: Foods

Article Title: Use of Doehlert Matrix as a Tool for High-Throughput Screening of Organic Acids and Essential Oils on Miniaturized Pork Loins, Followed by Lab-Scale Validation That Confirmed Tested Compounds Do Not Show Synergistic Effects against Salmonella Typhimurium

doi: 10.3390/foods12214034

Figure Lengend Snippet: Essential oil library screened for their individual antimicrobial activity against Salmonella Typhimurium ATCC 19585 on miniaturized pork disks.

Article Snippet: Then, 200 μL of stationary phase Salmonella Typhimurium ATCC 19585 inoculum, diluted to 10 CFU/mL (See ), was added onto the slice to reach a desired inoculum concentration of approximately 4-log (CFU/g).

Techniques: Activity Assay, Single-particle Tracking

Journal: Foods

Article Title: Use of Doehlert Matrix as a Tool for High-Throughput Screening of Organic Acids and Essential Oils on Miniaturized Pork Loins, Followed by Lab-Scale Validation That Confirmed Tested Compounds Do Not Show Synergistic Effects against Salmonella Typhimurium

doi: 10.3390/foods12214034

Figure Lengend Snippet: Antimicrobial screening of chemically polar compounds that were surface-applied to miniaturized pork disks previously inoculated with Salmonella Typhimurium ATCC 19585. Results are presented as the difference in cell counts after 24 h incubation at 4 °C; experiments were performed in biological triplicate. Antimicrobial treatments tested were formic acid (FA), lactic acid (LA), peracetic acid (PA), potassium sorbate (PS), and sodium propionate (SOP). Antimicrobial treatment concentrations are represented as % ( v / w ). Statistically significant treatments that had p < 0.05 from untreated sample have specific p -values annotated, while all other compounds had p > 0.05. Statistically significant compounds were identified through a one-way ANOVA followed by Dunnett’s multiple comparisons test.

Article Snippet: Then, 200 μL of stationary phase Salmonella Typhimurium ATCC 19585 inoculum, diluted to 10 CFU/mL (See ), was added onto the slice to reach a desired inoculum concentration of approximately 4-log (CFU/g).

Techniques: Incubation

Journal: Foods

Article Title: Use of Doehlert Matrix as a Tool for High-Throughput Screening of Organic Acids and Essential Oils on Miniaturized Pork Loins, Followed by Lab-Scale Validation That Confirmed Tested Compounds Do Not Show Synergistic Effects against Salmonella Typhimurium

doi: 10.3390/foods12214034

Figure Lengend Snippet: Antimicrobial screening of EO library. EOs were surface-applied to miniaturized pork disks previously inoculated with Salmonella Typhimurium ATCC 19585. Results are presented as the difference in cell counts after 24 h incubation at 4 °C. Experiments were carried out in biological duplicate. Statistically significant EOs were identified through a one-way ANOVA followed by Dunnett’s multiple comparisons test. Statistically significant treatments that had p < 0.05 from untreated sample have specific p -values annotated, while all other compounds had p > 0.05.

Article Snippet: Then, 200 μL of stationary phase Salmonella Typhimurium ATCC 19585 inoculum, diluted to 10 CFU/mL (See ), was added onto the slice to reach a desired inoculum concentration of approximately 4-log (CFU/g).

Techniques: Incubation

Journal: Antibiotics

Article Title: Ciprofloxacin and Tetracycline Resistance Cause Collateral Sensitivity to Aminoglycosides in Salmonella Typhimurium

doi: 10.3390/antibiotics12081335

Figure Lengend Snippet: Minimum inhibitory concentrations (MICs; µg/mL) of selected antibiotics against Salmonella Typhimurium ATCC 19585 (ST WT ), ciprofloxacin-resistant S . Typhimurium (ST CIP ), gentamicin-resistant S . Typhimurium (ST GEN ), kanamycin-resistant S . Typhimurium (ST KAN ), and tetracycline-induced resistant S . Typhimurium (ST TET ).

Article Snippet: S . Typhimurium ATCC 19585 (ST WT ) was exposed to ciprofloxacin, gentamicin, kanamycin, and tetracycline to induce antibiotic resistance, respectively, assigned as ST CIP , ST GEN , ST KAN , and ST TET .

Techniques:

Journal: Antibiotics

Article Title: Ciprofloxacin and Tetracycline Resistance Cause Collateral Sensitivity to Aminoglycosides in Salmonella Typhimurium

doi: 10.3390/antibiotics12081335

Figure Lengend Snippet: Heat map of fold changes in MIC R of ciprofloxacin-resistant Salmonella Typhimurium (ST CIP ), gentamicin-resistant S . Typhimurium (ST GEN ), kanamycin-resistant S . Typhimurium (ST KAN ), and tetracycline-induced resistant S . Typhimurium (ST TET ) compared to MIC WT of S . Typhimurium ATCC 19585 (ST WT ). Heat map intensities indicate the fold change in MIC compared to that of the untreated ST WT ; [log 2 MIC R /MIC W ]. CET, cefotaxime; CHL, chloramphenicol; GEN, gentamicin; KAN, kanamycin; PMB, polymyxin B; STR, streptomycin, TET; tetracycline; TOB, tobramycin.

Article Snippet: S . Typhimurium ATCC 19585 (ST WT ) was exposed to ciprofloxacin, gentamicin, kanamycin, and tetracycline to induce antibiotic resistance, respectively, assigned as ST CIP , ST GEN , ST KAN , and ST TET .

Techniques:

Journal: Nature microbiology

Article Title: Computational identification of a systemic antibiotic for gram-negative bacteria

doi: 10.1038/s41564-022-01227-4

Figure Lengend Snippet: Dynobactin A minimum inhibitory concentrations

Article Snippet: Salmonella enterica typhimurium LT2 ATCC 19585 , 8.

Techniques: