Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: An antagonist of the platelet-activating factor receptor inhibits adherence of both nontypeable Haemophilus influenzae and Streptococcus pneumoniae to cultured human bronchial epithelial cells exposed to cigarette smoke

doi: 10.2147/COPD.S108698

Figure Lengend Snippet: CSE increases the adhesion of the NTHi strains and S. pneumoniae to BEAS-2B cells. Notes: ( A ) Control BEAS-2B cells showing few FITC-tagged S. pneumoniae 132 cells adhering to BEAS-2B cells. ( B ) BEAS-2B cells exposed to CSE (1%) show increased attachment of FITC-tagged S. pneumoniae to BEAS-2B cells. All micrographs show BEAS-2B cells with PAFr expression (anti-PAFr monoclonal antibody; 1:100, red) and nuclei stained with 4′,6-diamidino-2-phenylindole (1:5,000, blue). Magnification =×400. ( C ) S. pneumoniae clinical isolate 132. ( D ) NTHi reference strain NCTC-4560. ( E ) NTHi clinical isolate RHH3. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; h, hours; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .

Article Snippet: NCTC-4560 (ATCC 19418), an NTHi laboratory reference strain, was also used.

Techniques: Expressing, Staining

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: An antagonist of the platelet-activating factor receptor inhibits adherence of both nontypeable Haemophilus influenzae and Streptococcus pneumoniae to cultured human bronchial epithelial cells exposed to cigarette smoke

doi: 10.2147/COPD.S108698

Figure Lengend Snippet: Correlation between change in PAFr protein expression and variation in the number of adherent bacteria to BEAS-2B cells. Notes: ( A ) S. pneumoniae clinical isolate 132. ( B ) NTHi reference strain NCTC-4560. ( C ) NTHi clinical isolate RHH3. Abbreviations: NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .

Article Snippet: NCTC-4560 (ATCC 19418), an NTHi laboratory reference strain, was also used.

Techniques: Expressing

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: An antagonist of the platelet-activating factor receptor inhibits adherence of both nontypeable Haemophilus influenzae and Streptococcus pneumoniae to cultured human bronchial epithelial cells exposed to cigarette smoke

doi: 10.2147/COPD.S108698

Figure Lengend Snippet: Inhibitory effect of WEB-2086 on the adhesion of bacterial strains to CSE-treated BEAS-2B cells. Notes: ( A ) BEAS-2B cells exposed to CSE (1%) showing attachment of FITC-tagged Streptococcus pneumoniae clinical isolate 132. ( B ) Reduced attachment of S. pneumoniae bacterial cells to CSE-treated BEAS-2B cells due to application of PAFr antagonist, WEB-2086. ( C ) Significant reductions in S. pneumoniae adhesion to CSE-treated cells were observed in the presence of 100 nM, 1 μM, and 10 μM WEB-2086. ( D ) A significant reduction in NTHi reference strain (NCTC-4560) adherence to CSE-treated cells was observed in the presence of 10 μM WEB-2086. ( E ) Significant reductions in NTHi clinical isolate (RHH3) adherence to CSE-treated cells were observed in the presence of 1 and 10 μM WEB-2086. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .

Article Snippet: NCTC-4560 (ATCC 19418), an NTHi laboratory reference strain, was also used.

Techniques:

Journal: PLoS ONE

Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

doi: 10.1371/journal.pone.0034083

Figure Lengend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

Article Snippet: DNA for standards was extracted from the reference strain ATCC 19418 and a dilution series ranging from 10 to 100000 genome copies per reaction was used to estimate the LOD of real time PCR assays.

Techniques: Sequencing, Amplification

Journal: PLoS ONE

Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

doi: 10.1371/journal.pone.0034083

Figure Lengend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

Article Snippet: Sequencing of the 16S rRNA and recA genes was performed on the 60 study isolates and 2 reference isolates ( H. influenzae - ATCC 19418, and H. haemolyticus - ATCC 33390) respectively yielding 598 and 543 unambiguous bases.

Techniques: Sequencing, Amplification

Journal: PLoS ONE

Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

doi: 10.1371/journal.pone.0034083

Figure Lengend Snippet: Radiation trees are presented for (A) 598 bases of the 16S rRNA gene, (B) 545 bases of the recA gene, and (C) the concatenation of these sequences (1143 bases). The trees are rooted by H. parainfluenzae T3T1 as indicated by yellow dots. Blue dots represent isolates that cluster with H. influenzae reference strains, orange dots represent closely related phylogenetic variants, red dots represent likely Hh isolates and green dots represent variants related to H. parainfluenzae . Colours were assigned based on the phylogenetic grouping of the concatenated sequences in radial tree C.

Article Snippet: The sequenced H. influenzae reference strain (ATCC 19418) grouped with the strict cluster of NTHi's by recA sequence but was less well defined by the 16S rRNA gene sequence; however, its sequence similarity across both 16S rRNA and recA gene sequences was sufficient to place it with the strict NTHi's on the concatenated tree.

Techniques:

Journal: PLoS ONE

Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

doi: 10.1371/journal.pone.0034083

Figure Lengend Snippet: Genbank reference strains.

Article Snippet: The sequenced H. influenzae reference strain (ATCC 19418) grouped with the strict cluster of NTHi's by recA sequence but was less well defined by the 16S rRNA gene sequence; however, its sequence similarity across both 16S rRNA and recA gene sequences was sufficient to place it with the strict NTHi's on the concatenated tree.

Techniques:

Journal: PLoS ONE

Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

doi: 10.1371/journal.pone.0034083

Figure Lengend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

Article Snippet: The sequenced H. influenzae reference strain (ATCC 19418) grouped with the strict cluster of NTHi's by recA sequence but was less well defined by the 16S rRNA gene sequence; however, its sequence similarity across both 16S rRNA and recA gene sequences was sufficient to place it with the strict NTHi's on the concatenated tree.

Techniques: Sequencing, Amplification

Journal: PLoS ONE

Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

doi: 10.1371/journal.pone.0034083

Figure Lengend Snippet: The Neighbour-Joining dendogram of the concatenated 16S rRNA and recA gene sequences is displayed in conjunction with the PCR results and the percent DNA similarity (compared to H. influenzae 86-028NP). The tree is rooted by H. parainfluenzae as indicated by the yellow dots. The blue dots represent the strict NTHi group, the orange dots represent closely related phylogenetic variants, the red dots represent likely Hh isolates and the green dots represent variants related to H. parainfluenzae . Study-defined NTHi was based collectively on the phylogeny, PCR data and DNA similarity as is indicated on the right (✓ = NTHi or ✗ = Hh).

Article Snippet: The sequenced H. influenzae reference strain (ATCC 19418) grouped with the strict cluster of NTHi's by recA sequence but was less well defined by the 16S rRNA gene sequence; however, its sequence similarity across both 16S rRNA and recA gene sequences was sufficient to place it with the strict NTHi's on the concatenated tree.

Techniques:

Journal: PLoS ONE

Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

doi: 10.1371/journal.pone.0034083

Figure Lengend Snippet: PCR screening of respiratory reference isolates.

Article Snippet: The sequenced H. influenzae reference strain (ATCC 19418) grouped with the strict cluster of NTHi's by recA sequence but was less well defined by the 16S rRNA gene sequence; however, its sequence similarity across both 16S rRNA and recA gene sequences was sufficient to place it with the strict NTHi's on the concatenated tree.

Techniques:

Journal: PLoS ONE

Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

doi: 10.1371/journal.pone.0034083

Figure Lengend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

Article Snippet: The upper group of curves included the H. influenzae reference strain ATCC 19418 and the lower group of curves included the Hh reference strain ATCC 33390.

Techniques: Sequencing, Amplification

Journal: Antibiotics

Article Title: Essential Oil Blends: The Potential of Combined Use for Respiratory Tract Infections

doi: 10.3390/antibiotics10121517

Figure Lengend Snippet: The mean MIC ( n = 3) with standard deviation in brackets and ΣFIC values of the essential oil combinations investigated against pathogens of the respiratory tract.

Article Snippet: Microbial cultures were selected based on their relevance to respiratory infections and included the Gram-positive strains Staphylococcus aureus (ATCC 25924), Streptococcus agalactiae (ATCC 55618), Streptococcus pneumoniae (ATCC 49619) and Streptococcus pyogenes (ATCC 12344) and the Gram-negative strains Haemophilus influenzae (ATCC 19418), Klebsiella pneumoniae (ATCC 13883) and Moraxella catarrhalis (ATCC 23246).

Techniques: Standard Deviation, Positive Control, Negative Control

Journal: Photochemical & Photobiological Sciences

Article Title: Photodynamic disinfection and its role in controlling infectious diseases

doi: 10.1007/s43630-021-00102-1

Figure Lengend Snippet: Chemical properties and biological activity of photosensitizers used in vivo/ex vivo pre-clinical studies for treatment of infections by Gram-negative bacteria

Article Snippet: 2 , Photogem (PS 31 ) Charge: − 2 to − 12 MW = n.d. , In vivo: Mongolian Gerbils with otitis caused by H. influenza ATCC 19418 [ ] , Outcome: complete reduction of H. influenza in 50% of infections [PS]: 1 mg/ml (20 μl) Light dose: n.d. total energy: 90 J (laser λ = 632 nm).

Techniques: Activity Assay, In Vivo, Infection, Ex Vivo, Cell Culture