nctc 4560  (ATCC)


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    ATCC nctc 4560
    CSE increases the adhesion of the NTHi strains and S. pneumoniae to BEAS-2B cells. Notes: ( A ) Control BEAS-2B cells showing few FITC-tagged S. pneumoniae 132 cells adhering to BEAS-2B cells. ( B ) BEAS-2B cells exposed to CSE (1%) show increased attachment of FITC-tagged S. pneumoniae to BEAS-2B cells. All micrographs show BEAS-2B cells with PAFr expression (anti-PAFr monoclonal antibody; 1:100, red) and nuclei stained with 4′,6-diamidino-2-phenylindole (1:5,000, blue). Magnification =×400. ( C ) S. pneumoniae clinical isolate 132. ( D ) NTHi reference strain <t>NCTC-4560.</t> ( E ) NTHi clinical isolate RHH3. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; h, hours; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .
    Nctc 4560, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "An antagonist of the platelet-activating factor receptor inhibits adherence of both nontypeable Haemophilus influenzae and Streptococcus pneumoniae to cultured human bronchial epithelial cells exposed to cigarette smoke"

    Article Title: An antagonist of the platelet-activating factor receptor inhibits adherence of both nontypeable Haemophilus influenzae and Streptococcus pneumoniae to cultured human bronchial epithelial cells exposed to cigarette smoke

    Journal: International Journal of Chronic Obstructive Pulmonary Disease

    doi: 10.2147/COPD.S108698

    CSE increases the adhesion of the NTHi strains and S. pneumoniae to BEAS-2B cells. Notes: ( A ) Control BEAS-2B cells showing few FITC-tagged S. pneumoniae 132 cells adhering to BEAS-2B cells. ( B ) BEAS-2B cells exposed to CSE (1%) show increased attachment of FITC-tagged S. pneumoniae to BEAS-2B cells. All micrographs show BEAS-2B cells with PAFr expression (anti-PAFr monoclonal antibody; 1:100, red) and nuclei stained with 4′,6-diamidino-2-phenylindole (1:5,000, blue). Magnification =×400. ( C ) S. pneumoniae clinical isolate 132. ( D ) NTHi reference strain NCTC-4560. ( E ) NTHi clinical isolate RHH3. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; h, hours; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .
    Figure Legend Snippet: CSE increases the adhesion of the NTHi strains and S. pneumoniae to BEAS-2B cells. Notes: ( A ) Control BEAS-2B cells showing few FITC-tagged S. pneumoniae 132 cells adhering to BEAS-2B cells. ( B ) BEAS-2B cells exposed to CSE (1%) show increased attachment of FITC-tagged S. pneumoniae to BEAS-2B cells. All micrographs show BEAS-2B cells with PAFr expression (anti-PAFr monoclonal antibody; 1:100, red) and nuclei stained with 4′,6-diamidino-2-phenylindole (1:5,000, blue). Magnification =×400. ( C ) S. pneumoniae clinical isolate 132. ( D ) NTHi reference strain NCTC-4560. ( E ) NTHi clinical isolate RHH3. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; h, hours; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .

    Techniques Used: Expressing, Staining

    Correlation between change in PAFr protein expression and variation in the number of adherent bacteria to BEAS-2B cells. Notes: ( A ) S. pneumoniae clinical isolate 132. ( B ) NTHi reference strain NCTC-4560. ( C ) NTHi clinical isolate RHH3. Abbreviations: NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .
    Figure Legend Snippet: Correlation between change in PAFr protein expression and variation in the number of adherent bacteria to BEAS-2B cells. Notes: ( A ) S. pneumoniae clinical isolate 132. ( B ) NTHi reference strain NCTC-4560. ( C ) NTHi clinical isolate RHH3. Abbreviations: NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .

    Techniques Used: Expressing

    Inhibitory effect of WEB-2086 on the adhesion of bacterial strains to CSE-treated BEAS-2B cells. Notes: ( A ) BEAS-2B cells exposed to CSE (1%) showing attachment of FITC-tagged Streptococcus pneumoniae clinical isolate 132. ( B ) Reduced attachment of S. pneumoniae bacterial cells to CSE-treated BEAS-2B cells due to application of PAFr antagonist, WEB-2086. ( C ) Significant reductions in S. pneumoniae adhesion to CSE-treated cells were observed in the presence of 100 nM, 1 μM, and 10 μM WEB-2086. ( D ) A significant reduction in NTHi reference strain (NCTC-4560) adherence to CSE-treated cells was observed in the presence of 10 μM WEB-2086. ( E ) Significant reductions in NTHi clinical isolate (RHH3) adherence to CSE-treated cells were observed in the presence of 1 and 10 μM WEB-2086. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .
    Figure Legend Snippet: Inhibitory effect of WEB-2086 on the adhesion of bacterial strains to CSE-treated BEAS-2B cells. Notes: ( A ) BEAS-2B cells exposed to CSE (1%) showing attachment of FITC-tagged Streptococcus pneumoniae clinical isolate 132. ( B ) Reduced attachment of S. pneumoniae bacterial cells to CSE-treated BEAS-2B cells due to application of PAFr antagonist, WEB-2086. ( C ) Significant reductions in S. pneumoniae adhesion to CSE-treated cells were observed in the presence of 100 nM, 1 μM, and 10 μM WEB-2086. ( D ) A significant reduction in NTHi reference strain (NCTC-4560) adherence to CSE-treated cells was observed in the presence of 10 μM WEB-2086. ( E ) Significant reductions in NTHi clinical isolate (RHH3) adherence to CSE-treated cells were observed in the presence of 1 and 10 μM WEB-2086. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .

    Techniques Used:

    nthi 19418  (ATCC)


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    ATCC nthi 19418
    Nthi 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nthi 19418  (ATCC)


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    ATCC nthi 19418
    Nthi 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    haemophilus influenzae atcc 19418  (ATCC)


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    ATCC haemophilus influenzae atcc 19418
    Haemophilus Influenzae Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nctc 4560  (ATCC)


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    ATCC nctc 4560
    CSE increases the adhesion of the NTHi strains and S. pneumoniae to BEAS-2B cells. Notes: ( A ) Control BEAS-2B cells showing few FITC-tagged S. pneumoniae 132 cells adhering to BEAS-2B cells. ( B ) BEAS-2B cells exposed to CSE (1%) show increased attachment of FITC-tagged S. pneumoniae to BEAS-2B cells. All micrographs show BEAS-2B cells with PAFr expression (anti-PAFr monoclonal antibody; 1:100, red) and nuclei stained with 4′,6-diamidino-2-phenylindole (1:5,000, blue). Magnification =×400. ( C ) S. pneumoniae clinical isolate 132. ( D ) NTHi reference strain <t>NCTC-4560.</t> ( E ) NTHi clinical isolate RHH3. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; h, hours; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .
    Nctc 4560, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "An antagonist of the platelet-activating factor receptor inhibits adherence of both nontypeable Haemophilus influenzae and Streptococcus pneumoniae to cultured human bronchial epithelial cells exposed to cigarette smoke"

    Article Title: An antagonist of the platelet-activating factor receptor inhibits adherence of both nontypeable Haemophilus influenzae and Streptococcus pneumoniae to cultured human bronchial epithelial cells exposed to cigarette smoke

    Journal: International Journal of Chronic Obstructive Pulmonary Disease

    doi: 10.2147/COPD.S108698

    CSE increases the adhesion of the NTHi strains and S. pneumoniae to BEAS-2B cells. Notes: ( A ) Control BEAS-2B cells showing few FITC-tagged S. pneumoniae 132 cells adhering to BEAS-2B cells. ( B ) BEAS-2B cells exposed to CSE (1%) show increased attachment of FITC-tagged S. pneumoniae to BEAS-2B cells. All micrographs show BEAS-2B cells with PAFr expression (anti-PAFr monoclonal antibody; 1:100, red) and nuclei stained with 4′,6-diamidino-2-phenylindole (1:5,000, blue). Magnification =×400. ( C ) S. pneumoniae clinical isolate 132. ( D ) NTHi reference strain NCTC-4560. ( E ) NTHi clinical isolate RHH3. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; h, hours; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .
    Figure Legend Snippet: CSE increases the adhesion of the NTHi strains and S. pneumoniae to BEAS-2B cells. Notes: ( A ) Control BEAS-2B cells showing few FITC-tagged S. pneumoniae 132 cells adhering to BEAS-2B cells. ( B ) BEAS-2B cells exposed to CSE (1%) show increased attachment of FITC-tagged S. pneumoniae to BEAS-2B cells. All micrographs show BEAS-2B cells with PAFr expression (anti-PAFr monoclonal antibody; 1:100, red) and nuclei stained with 4′,6-diamidino-2-phenylindole (1:5,000, blue). Magnification =×400. ( C ) S. pneumoniae clinical isolate 132. ( D ) NTHi reference strain NCTC-4560. ( E ) NTHi clinical isolate RHH3. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; h, hours; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .

    Techniques Used: Expressing, Staining

    Correlation between change in PAFr protein expression and variation in the number of adherent bacteria to BEAS-2B cells. Notes: ( A ) S. pneumoniae clinical isolate 132. ( B ) NTHi reference strain NCTC-4560. ( C ) NTHi clinical isolate RHH3. Abbreviations: NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .
    Figure Legend Snippet: Correlation between change in PAFr protein expression and variation in the number of adherent bacteria to BEAS-2B cells. Notes: ( A ) S. pneumoniae clinical isolate 132. ( B ) NTHi reference strain NCTC-4560. ( C ) NTHi clinical isolate RHH3. Abbreviations: NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .

    Techniques Used: Expressing

    Inhibitory effect of WEB-2086 on the adhesion of bacterial strains to CSE-treated BEAS-2B cells. Notes: ( A ) BEAS-2B cells exposed to CSE (1%) showing attachment of FITC-tagged Streptococcus pneumoniae clinical isolate 132. ( B ) Reduced attachment of S. pneumoniae bacterial cells to CSE-treated BEAS-2B cells due to application of PAFr antagonist, WEB-2086. ( C ) Significant reductions in S. pneumoniae adhesion to CSE-treated cells were observed in the presence of 100 nM, 1 μM, and 10 μM WEB-2086. ( D ) A significant reduction in NTHi reference strain (NCTC-4560) adherence to CSE-treated cells was observed in the presence of 10 μM WEB-2086. ( E ) Significant reductions in NTHi clinical isolate (RHH3) adherence to CSE-treated cells were observed in the presence of 1 and 10 μM WEB-2086. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .
    Figure Legend Snippet: Inhibitory effect of WEB-2086 on the adhesion of bacterial strains to CSE-treated BEAS-2B cells. Notes: ( A ) BEAS-2B cells exposed to CSE (1%) showing attachment of FITC-tagged Streptococcus pneumoniae clinical isolate 132. ( B ) Reduced attachment of S. pneumoniae bacterial cells to CSE-treated BEAS-2B cells due to application of PAFr antagonist, WEB-2086. ( C ) Significant reductions in S. pneumoniae adhesion to CSE-treated cells were observed in the presence of 100 nM, 1 μM, and 10 μM WEB-2086. ( D ) A significant reduction in NTHi reference strain (NCTC-4560) adherence to CSE-treated cells was observed in the presence of 10 μM WEB-2086. ( E ) Significant reductions in NTHi clinical isolate (RHH3) adherence to CSE-treated cells were observed in the presence of 1 and 10 μM WEB-2086. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .

    Techniques Used:

    haemophilus influenzae 19418  (ATCC)


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    ATCC haemophilus influenzae 19418
    Haemophilus Influenzae 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    reference strain atcc 19418  (ATCC)


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    ATCC reference strain atcc 19418
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Reference Strain Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays"

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0034083

    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Figure Legend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Techniques Used: Sequencing, Amplification

    influenzae atcc 19418  (ATCC)


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    ATCC influenzae atcc 19418
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Influenzae Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    influenzae atcc 19418 - by Bioz Stars, 2024-02
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    1) Product Images from "Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays"

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0034083

    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Figure Legend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Techniques Used: Sequencing, Amplification

    influenzae reference strain  (ATCC)


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    ATCC influenzae reference strain
    Radiation trees are presented for (A) 598 bases of the 16S rRNA gene, (B) 545 bases of the recA gene, and (C) the concatenation of these sequences (1143 bases). The trees are rooted by H. parainfluenzae T3T1 as indicated by yellow dots. Blue dots represent isolates that cluster with H. <t>influenzae</t> reference strains, orange dots represent closely related phylogenetic variants, red dots represent likely Hh isolates and green dots represent variants related to H. parainfluenzae . Colours were assigned based on the phylogenetic grouping of the concatenated sequences in radial tree C.
    Influenzae Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays"

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0034083

    Radiation trees are presented for (A) 598 bases of the 16S rRNA gene, (B) 545 bases of the recA gene, and (C) the concatenation of these sequences (1143 bases). The trees are rooted by H. parainfluenzae T3T1 as indicated by yellow dots. Blue dots represent isolates that cluster with H. influenzae reference strains, orange dots represent closely related phylogenetic variants, red dots represent likely Hh isolates and green dots represent variants related to H. parainfluenzae . Colours were assigned based on the phylogenetic grouping of the concatenated sequences in radial tree C.
    Figure Legend Snippet: Radiation trees are presented for (A) 598 bases of the 16S rRNA gene, (B) 545 bases of the recA gene, and (C) the concatenation of these sequences (1143 bases). The trees are rooted by H. parainfluenzae T3T1 as indicated by yellow dots. Blue dots represent isolates that cluster with H. influenzae reference strains, orange dots represent closely related phylogenetic variants, red dots represent likely Hh isolates and green dots represent variants related to H. parainfluenzae . Colours were assigned based on the phylogenetic grouping of the concatenated sequences in radial tree C.

    Techniques Used:

    Genbank reference strains.
    Figure Legend Snippet: Genbank reference strains.

    Techniques Used:

    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Figure Legend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Techniques Used: Sequencing, Amplification

    The Neighbour-Joining dendogram of the concatenated 16S rRNA and recA gene sequences is displayed in conjunction with the PCR results and the percent DNA similarity (compared to H. influenzae 86-028NP). The tree is rooted by H. parainfluenzae as indicated by the yellow dots. The blue dots represent the strict NTHi group, the orange dots represent closely related phylogenetic variants, the red dots represent likely Hh isolates and the green dots represent variants related to H. parainfluenzae . Study-defined NTHi was based collectively on the phylogeny, PCR data and DNA similarity as is indicated on the right (✓ = NTHi or ✗ = Hh).
    Figure Legend Snippet: The Neighbour-Joining dendogram of the concatenated 16S rRNA and recA gene sequences is displayed in conjunction with the PCR results and the percent DNA similarity (compared to H. influenzae 86-028NP). The tree is rooted by H. parainfluenzae as indicated by the yellow dots. The blue dots represent the strict NTHi group, the orange dots represent closely related phylogenetic variants, the red dots represent likely Hh isolates and the green dots represent variants related to H. parainfluenzae . Study-defined NTHi was based collectively on the phylogeny, PCR data and DNA similarity as is indicated on the right (✓ = NTHi or ✗ = Hh).

    Techniques Used:

    PCR screening of respiratory reference isolates.
    Figure Legend Snippet: PCR screening of respiratory reference isolates.

    Techniques Used:

    influenzae reference strain atcc 19418  (ATCC)


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    ATCC influenzae reference strain atcc 19418
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Influenzae Reference Strain Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    influenzae reference strain atcc 19418 - by Bioz Stars, 2024-02
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    1) Product Images from "Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays"

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0034083

    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Figure Legend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Techniques Used: Sequencing, Amplification

    influenzae  (ATCC)


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    ATCC influenzae
    Influenzae, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC nctc 4560
    CSE increases the adhesion of the NTHi strains and S. pneumoniae to BEAS-2B cells. Notes: ( A ) Control BEAS-2B cells showing few FITC-tagged S. pneumoniae 132 cells adhering to BEAS-2B cells. ( B ) BEAS-2B cells exposed to CSE (1%) show increased attachment of FITC-tagged S. pneumoniae to BEAS-2B cells. All micrographs show BEAS-2B cells with PAFr expression (anti-PAFr monoclonal antibody; 1:100, red) and nuclei stained with 4′,6-diamidino-2-phenylindole (1:5,000, blue). Magnification =×400. ( C ) S. pneumoniae clinical isolate 132. ( D ) NTHi reference strain <t>NCTC-4560.</t> ( E ) NTHi clinical isolate RHH3. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; h, hours; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .
    Nctc 4560, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC nthi 19418
    CSE increases the adhesion of the NTHi strains and S. pneumoniae to BEAS-2B cells. Notes: ( A ) Control BEAS-2B cells showing few FITC-tagged S. pneumoniae 132 cells adhering to BEAS-2B cells. ( B ) BEAS-2B cells exposed to CSE (1%) show increased attachment of FITC-tagged S. pneumoniae to BEAS-2B cells. All micrographs show BEAS-2B cells with PAFr expression (anti-PAFr monoclonal antibody; 1:100, red) and nuclei stained with 4′,6-diamidino-2-phenylindole (1:5,000, blue). Magnification =×400. ( C ) S. pneumoniae clinical isolate 132. ( D ) NTHi reference strain <t>NCTC-4560.</t> ( E ) NTHi clinical isolate RHH3. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; h, hours; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .
    Nthi 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC haemophilus influenzae atcc 19418
    CSE increases the adhesion of the NTHi strains and S. pneumoniae to BEAS-2B cells. Notes: ( A ) Control BEAS-2B cells showing few FITC-tagged S. pneumoniae 132 cells adhering to BEAS-2B cells. ( B ) BEAS-2B cells exposed to CSE (1%) show increased attachment of FITC-tagged S. pneumoniae to BEAS-2B cells. All micrographs show BEAS-2B cells with PAFr expression (anti-PAFr monoclonal antibody; 1:100, red) and nuclei stained with 4′,6-diamidino-2-phenylindole (1:5,000, blue). Magnification =×400. ( C ) S. pneumoniae clinical isolate 132. ( D ) NTHi reference strain <t>NCTC-4560.</t> ( E ) NTHi clinical isolate RHH3. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; h, hours; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .
    Haemophilus Influenzae Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC haemophilus influenzae 19418
    CSE increases the adhesion of the NTHi strains and S. pneumoniae to BEAS-2B cells. Notes: ( A ) Control BEAS-2B cells showing few FITC-tagged S. pneumoniae 132 cells adhering to BEAS-2B cells. ( B ) BEAS-2B cells exposed to CSE (1%) show increased attachment of FITC-tagged S. pneumoniae to BEAS-2B cells. All micrographs show BEAS-2B cells with PAFr expression (anti-PAFr monoclonal antibody; 1:100, red) and nuclei stained with 4′,6-diamidino-2-phenylindole (1:5,000, blue). Magnification =×400. ( C ) S. pneumoniae clinical isolate 132. ( D ) NTHi reference strain <t>NCTC-4560.</t> ( E ) NTHi clinical isolate RHH3. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; h, hours; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .
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    ATCC reference strain atcc 19418
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Reference Strain Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC influenzae atcc 19418
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Influenzae Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC influenzae reference strain
    Radiation trees are presented for (A) 598 bases of the 16S rRNA gene, (B) 545 bases of the recA gene, and (C) the concatenation of these sequences (1143 bases). The trees are rooted by H. parainfluenzae T3T1 as indicated by yellow dots. Blue dots represent isolates that cluster with H. <t>influenzae</t> reference strains, orange dots represent closely related phylogenetic variants, red dots represent likely Hh isolates and green dots represent variants related to H. parainfluenzae . Colours were assigned based on the phylogenetic grouping of the concatenated sequences in radial tree C.
    Influenzae Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC influenzae reference strain atcc 19418
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Influenzae Reference Strain Atcc 19418, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC influenzae
    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).
    Influenzae, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CSE increases the adhesion of the NTHi strains and S. pneumoniae to BEAS-2B cells. Notes: ( A ) Control BEAS-2B cells showing few FITC-tagged S. pneumoniae 132 cells adhering to BEAS-2B cells. ( B ) BEAS-2B cells exposed to CSE (1%) show increased attachment of FITC-tagged S. pneumoniae to BEAS-2B cells. All micrographs show BEAS-2B cells with PAFr expression (anti-PAFr monoclonal antibody; 1:100, red) and nuclei stained with 4′,6-diamidino-2-phenylindole (1:5,000, blue). Magnification =×400. ( C ) S. pneumoniae clinical isolate 132. ( D ) NTHi reference strain NCTC-4560. ( E ) NTHi clinical isolate RHH3. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; h, hours; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .

    Journal: International Journal of Chronic Obstructive Pulmonary Disease

    Article Title: An antagonist of the platelet-activating factor receptor inhibits adherence of both nontypeable Haemophilus influenzae and Streptococcus pneumoniae to cultured human bronchial epithelial cells exposed to cigarette smoke

    doi: 10.2147/COPD.S108698

    Figure Lengend Snippet: CSE increases the adhesion of the NTHi strains and S. pneumoniae to BEAS-2B cells. Notes: ( A ) Control BEAS-2B cells showing few FITC-tagged S. pneumoniae 132 cells adhering to BEAS-2B cells. ( B ) BEAS-2B cells exposed to CSE (1%) show increased attachment of FITC-tagged S. pneumoniae to BEAS-2B cells. All micrographs show BEAS-2B cells with PAFr expression (anti-PAFr monoclonal antibody; 1:100, red) and nuclei stained with 4′,6-diamidino-2-phenylindole (1:5,000, blue). Magnification =×400. ( C ) S. pneumoniae clinical isolate 132. ( D ) NTHi reference strain NCTC-4560. ( E ) NTHi clinical isolate RHH3. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; h, hours; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .

    Article Snippet: NCTC-4560 (ATCC 19418), an NTHi laboratory reference strain, was also used.

    Techniques: Expressing, Staining

    Correlation between change in PAFr protein expression and variation in the number of adherent bacteria to BEAS-2B cells. Notes: ( A ) S. pneumoniae clinical isolate 132. ( B ) NTHi reference strain NCTC-4560. ( C ) NTHi clinical isolate RHH3. Abbreviations: NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .

    Journal: International Journal of Chronic Obstructive Pulmonary Disease

    Article Title: An antagonist of the platelet-activating factor receptor inhibits adherence of both nontypeable Haemophilus influenzae and Streptococcus pneumoniae to cultured human bronchial epithelial cells exposed to cigarette smoke

    doi: 10.2147/COPD.S108698

    Figure Lengend Snippet: Correlation between change in PAFr protein expression and variation in the number of adherent bacteria to BEAS-2B cells. Notes: ( A ) S. pneumoniae clinical isolate 132. ( B ) NTHi reference strain NCTC-4560. ( C ) NTHi clinical isolate RHH3. Abbreviations: NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .

    Article Snippet: NCTC-4560 (ATCC 19418), an NTHi laboratory reference strain, was also used.

    Techniques: Expressing

    Inhibitory effect of WEB-2086 on the adhesion of bacterial strains to CSE-treated BEAS-2B cells. Notes: ( A ) BEAS-2B cells exposed to CSE (1%) showing attachment of FITC-tagged Streptococcus pneumoniae clinical isolate 132. ( B ) Reduced attachment of S. pneumoniae bacterial cells to CSE-treated BEAS-2B cells due to application of PAFr antagonist, WEB-2086. ( C ) Significant reductions in S. pneumoniae adhesion to CSE-treated cells were observed in the presence of 100 nM, 1 μM, and 10 μM WEB-2086. ( D ) A significant reduction in NTHi reference strain (NCTC-4560) adherence to CSE-treated cells was observed in the presence of 10 μM WEB-2086. ( E ) Significant reductions in NTHi clinical isolate (RHH3) adherence to CSE-treated cells were observed in the presence of 1 and 10 μM WEB-2086. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .

    Journal: International Journal of Chronic Obstructive Pulmonary Disease

    Article Title: An antagonist of the platelet-activating factor receptor inhibits adherence of both nontypeable Haemophilus influenzae and Streptococcus pneumoniae to cultured human bronchial epithelial cells exposed to cigarette smoke

    doi: 10.2147/COPD.S108698

    Figure Lengend Snippet: Inhibitory effect of WEB-2086 on the adhesion of bacterial strains to CSE-treated BEAS-2B cells. Notes: ( A ) BEAS-2B cells exposed to CSE (1%) showing attachment of FITC-tagged Streptococcus pneumoniae clinical isolate 132. ( B ) Reduced attachment of S. pneumoniae bacterial cells to CSE-treated BEAS-2B cells due to application of PAFr antagonist, WEB-2086. ( C ) Significant reductions in S. pneumoniae adhesion to CSE-treated cells were observed in the presence of 100 nM, 1 μM, and 10 μM WEB-2086. ( D ) A significant reduction in NTHi reference strain (NCTC-4560) adherence to CSE-treated cells was observed in the presence of 10 μM WEB-2086. ( E ) Significant reductions in NTHi clinical isolate (RHH3) adherence to CSE-treated cells were observed in the presence of 1 and 10 μM WEB-2086. Bars represent standard error of the mean. Abbreviations: CSE, cigarette smoke extract; FITC, fluorescein isothiocyanate; NTHi, non-typeable Haemophilus influenzae ; PAFr, platelet-activating factor receptor; S. pneumoniae , Streptococcus pneumoniae .

    Article Snippet: NCTC-4560 (ATCC 19418), an NTHi laboratory reference strain, was also used.

    Techniques:

    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Journal: PLoS ONE

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    doi: 10.1371/journal.pone.0034083

    Figure Lengend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Article Snippet: DNA for standards was extracted from the reference strain ATCC 19418 and a dilution series ranging from 10 to 100000 genome copies per reaction was used to estimate the LOD of real time PCR assays.

    Techniques: Sequencing, Amplification

    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Journal: PLoS ONE

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    doi: 10.1371/journal.pone.0034083

    Figure Lengend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Article Snippet: Sequencing of the 16S rRNA and recA genes was performed on the 60 study isolates and 2 reference isolates ( H. influenzae - ATCC 19418, and H. haemolyticus - ATCC 33390) respectively yielding 598 and 543 unambiguous bases.

    Techniques: Sequencing, Amplification

    Radiation trees are presented for (A) 598 bases of the 16S rRNA gene, (B) 545 bases of the recA gene, and (C) the concatenation of these sequences (1143 bases). The trees are rooted by H. parainfluenzae T3T1 as indicated by yellow dots. Blue dots represent isolates that cluster with H. influenzae reference strains, orange dots represent closely related phylogenetic variants, red dots represent likely Hh isolates and green dots represent variants related to H. parainfluenzae . Colours were assigned based on the phylogenetic grouping of the concatenated sequences in radial tree C.

    Journal: PLoS ONE

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    doi: 10.1371/journal.pone.0034083

    Figure Lengend Snippet: Radiation trees are presented for (A) 598 bases of the 16S rRNA gene, (B) 545 bases of the recA gene, and (C) the concatenation of these sequences (1143 bases). The trees are rooted by H. parainfluenzae T3T1 as indicated by yellow dots. Blue dots represent isolates that cluster with H. influenzae reference strains, orange dots represent closely related phylogenetic variants, red dots represent likely Hh isolates and green dots represent variants related to H. parainfluenzae . Colours were assigned based on the phylogenetic grouping of the concatenated sequences in radial tree C.

    Article Snippet: The sequenced H. influenzae reference strain (ATCC 19418) grouped with the strict cluster of NTHi's by recA sequence but was less well defined by the 16S rRNA gene sequence; however, its sequence similarity across both 16S rRNA and recA gene sequences was sufficient to place it with the strict NTHi's on the concatenated tree.

    Techniques:

    Genbank reference strains.

    Journal: PLoS ONE

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    doi: 10.1371/journal.pone.0034083

    Figure Lengend Snippet: Genbank reference strains.

    Article Snippet: The sequenced H. influenzae reference strain (ATCC 19418) grouped with the strict cluster of NTHi's by recA sequence but was less well defined by the 16S rRNA gene sequence; however, its sequence similarity across both 16S rRNA and recA gene sequences was sufficient to place it with the strict NTHi's on the concatenated tree.

    Techniques:

    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Journal: PLoS ONE

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    doi: 10.1371/journal.pone.0034083

    Figure Lengend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Article Snippet: The sequenced H. influenzae reference strain (ATCC 19418) grouped with the strict cluster of NTHi's by recA sequence but was less well defined by the 16S rRNA gene sequence; however, its sequence similarity across both 16S rRNA and recA gene sequences was sufficient to place it with the strict NTHi's on the concatenated tree.

    Techniques: Sequencing, Amplification

    The Neighbour-Joining dendogram of the concatenated 16S rRNA and recA gene sequences is displayed in conjunction with the PCR results and the percent DNA similarity (compared to H. influenzae 86-028NP). The tree is rooted by H. parainfluenzae as indicated by the yellow dots. The blue dots represent the strict NTHi group, the orange dots represent closely related phylogenetic variants, the red dots represent likely Hh isolates and the green dots represent variants related to H. parainfluenzae . Study-defined NTHi was based collectively on the phylogeny, PCR data and DNA similarity as is indicated on the right (✓ = NTHi or ✗ = Hh).

    Journal: PLoS ONE

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    doi: 10.1371/journal.pone.0034083

    Figure Lengend Snippet: The Neighbour-Joining dendogram of the concatenated 16S rRNA and recA gene sequences is displayed in conjunction with the PCR results and the percent DNA similarity (compared to H. influenzae 86-028NP). The tree is rooted by H. parainfluenzae as indicated by the yellow dots. The blue dots represent the strict NTHi group, the orange dots represent closely related phylogenetic variants, the red dots represent likely Hh isolates and the green dots represent variants related to H. parainfluenzae . Study-defined NTHi was based collectively on the phylogeny, PCR data and DNA similarity as is indicated on the right (✓ = NTHi or ✗ = Hh).

    Article Snippet: The sequenced H. influenzae reference strain (ATCC 19418) grouped with the strict cluster of NTHi's by recA sequence but was less well defined by the 16S rRNA gene sequence; however, its sequence similarity across both 16S rRNA and recA gene sequences was sufficient to place it with the strict NTHi's on the concatenated tree.

    Techniques:

    PCR screening of respiratory reference isolates.

    Journal: PLoS ONE

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    doi: 10.1371/journal.pone.0034083

    Figure Lengend Snippet: PCR screening of respiratory reference isolates.

    Article Snippet: The sequenced H. influenzae reference strain (ATCC 19418) grouped with the strict cluster of NTHi's by recA sequence but was less well defined by the 16S rRNA gene sequence; however, its sequence similarity across both 16S rRNA and recA gene sequences was sufficient to place it with the strict NTHi's on the concatenated tree.

    Techniques:

    Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Journal: PLoS ONE

    Article Title: Molecular Surveillance of True Nontypeable Haemophilus influenzae : An Evaluation of PCR Screening Assays

    doi: 10.1371/journal.pone.0034083

    Figure Lengend Snippet: Analysis of publically available sequence identified a SNP in the omp P6 genes of NTHi (G) and Hh (T) corresponding to nucleotide position 402465 of H. influenzae Rd KW20, Accession No. L42023. HRM of the 40 base pair amplicon (402446–402485) surrounding the SNP revealed 2 discrete melt profiles; one that clustered with reference H. influenzae (ATCC 19418) and another that clustered with reference Hh (ATCC 33390).

    Article Snippet: The upper group of curves included the H. influenzae reference strain ATCC 19418 and the lower group of curves included the Hh reference strain ATCC 33390.

    Techniques: Sequencing, Amplification