Rubrisubalbicans, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Quantification of natural populations of Gluconacetobacter diazotrophicus and Herbaspirillum spp. In sugar cane (Saccharum spp.) Using differente polyclonal antibodies"
Article Title: Quantification of natural populations of Gluconacetobacter diazotrophicus and Herbaspirillum spp. In sugar cane (Saccharum spp.) Using differente polyclonal antibodies
Journal: Brazilian Journal of Microbiology
Figure Legend Snippet: Titre of the polyclonal antibodies produced against the species G. diazotrophicus, H. seropedicae and H. rubrisubalbicans . Primary antibodies (PA) used: anti-PR2 [GB-pH 3.0 (ptn 0.640 mg mL -1 ) and GB-pH 4.0 (ptn 0.205 mg mL- 1 )]; anti-PAL3 [GB-pH 3.0 (ptn 0.255 mg mL -1 ) and GB-pH 4.0 (ptn 0.342 mg mL -1 )]; anti-Z 67 [GB-pH 3.0 (ptn 0.473 mg mL -1 ) and GB-pH 4.0 (ptn 0.362 mg mL -1 )]; anti-HRC54 [GB-pH 3.0 (ptn 0.115 mg mL -1 ) and GB-pH 4.0 (ptn 0.285 mg mL- 1 )]; anti-M4 [GB-pH 3.0 (ptn 0.609 mg mL -1 ) and GB-pH 4.0 (ptn– 0.382 mg mL -1 )]; anti-HCC103 [GB-pH 3 .0 (ptn 0.117 mg mL -1 ) and GB-pH 4.0 (ptn 0. 330 mg mL -1 )]. The concentrations of antigens used were 10 8 cell mL -1 . Secondary antibody (SA) dilution was 1:300. The incubations with the antibodies were processed at 37 ºC for 30 min to PA and for 45 min to SA, after rotation at 300 rpm for 3 min. Reaction time with the ABTS substrate were inferior for 9 min at 100 rpm. Means of 4 replicates. The absorbance of the pre-immune serum was subtracted from the absorbance of the samples. Absorbance read at λ= 405 nm.
Techniques Used: Produced