Journal: Brazilian Journal of Microbiology

Article Title: Quantification of natural populations of Gluconacetobacter diazotrophicus and Herbaspirillum spp. In sugar cane ( Saccharum spp.) Using differente polyclonal antibodies

doi: 10.1590/S1517-838220090004000018

Figure Lengend Snippet: Cross reaction values of different strains of N2-fixing bacteria against Gluconacetobacter diazotrophicus antiserum.

Article Snippet: For H. rubrisubalbicans , type strain M4 (BR 11192, ATCC 19308, DSM 9440, LMG 2286) isolated from sugar cane in the USA was used, along with HCC103 (BR 11504) isolated from roots of sugar cane in Brazil.

Techniques: Purification

Journal: Brazilian Journal of Microbiology

Article Title: Quantification of natural populations of Gluconacetobacter diazotrophicus and Herbaspirillum spp. In sugar cane ( Saccharum spp.) Using differente polyclonal antibodies

doi: 10.1590/S1517-838220090004000018

Figure Lengend Snippet: Cross reaction values of different strains of N 2 -fixing bacteria against Herbaspirillum seropedicae antiserum

Article Snippet: For H. rubrisubalbicans , type strain M4 (BR 11192, ATCC 19308, DSM 9440, LMG 2286) isolated from sugar cane in the USA was used, along with HCC103 (BR 11504) isolated from roots of sugar cane in Brazil.

Techniques: Purification

Journal: Brazilian Journal of Microbiology

Article Title: Quantification of natural populations of Gluconacetobacter diazotrophicus and Herbaspirillum spp. In sugar cane ( Saccharum spp.) Using differente polyclonal antibodies

doi: 10.1590/S1517-838220090004000018

Figure Lengend Snippet: Cross reaction values of different strains of N2-fixing bacteria against Herbaspirillum rubrisubalbicans antiserum.

Article Snippet: For H. rubrisubalbicans , type strain M4 (BR 11192, ATCC 19308, DSM 9440, LMG 2286) isolated from sugar cane in the USA was used, along with HCC103 (BR 11504) isolated from roots of sugar cane in Brazil.

Techniques: Purification

Journal: Brazilian Journal of Microbiology

Article Title: Quantification of natural populations of Gluconacetobacter diazotrophicus and Herbaspirillum spp. In sugar cane ( Saccharum spp.) Using differente polyclonal antibodies

doi: 10.1590/S1517-838220090004000018

Figure Lengend Snippet: Titre of the polyclonal antibodies produced against the species G. diazotrophicus, H. seropedicae and H. rubrisubalbicans . Primary antibodies (PA) used: anti-PR2 [GB-pH 3.0 (ptn 0.640 mg mL -1 ) and GB-pH 4.0 (ptn 0.205 mg mL- 1 )]; anti-PAL3 [GB-pH 3.0 (ptn 0.255 mg mL -1 ) and GB-pH 4.0 (ptn 0.342 mg mL -1 )]; anti-Z 67 [GB-pH 3.0 (ptn 0.473 mg mL -1 ) and GB-pH 4.0 (ptn 0.362 mg mL -1 )]; anti-HRC54 [GB-pH 3.0 (ptn 0.115 mg mL -1 ) and GB-pH 4.0 (ptn 0.285 mg mL- 1 )]; anti-M4 [GB-pH 3.0 (ptn 0.609 mg mL -1 ) and GB-pH 4.0 (ptn– 0.382 mg mL -1 )]; anti-HCC103 [GB-pH 3 .0 (ptn 0.117 mg mL -1 ) and GB-pH 4.0 (ptn 0. 330 mg mL -1 )]. The concentrations of antigens used were 10 8 cell mL -1 . Secondary antibody (SA) dilution was 1:300. The incubations with the antibodies were processed at 37 ºC for 30 min to PA and for 45 min to SA, after rotation at 300 rpm for 3 min. Reaction time with the ABTS substrate were inferior for 9 min at 100 rpm. Means of 4 replicates. The absorbance of the pre-immune serum was subtracted from the absorbance of the samples. Absorbance read at λ= 405 nm.

Article Snippet: For H. rubrisubalbicans , type strain M4 (BR 11192, ATCC 19308, DSM 9440, LMG 2286) isolated from sugar cane in the USA was used, along with HCC103 (BR 11504) isolated from roots of sugar cane in Brazil.

Techniques: Produced