am2  (ATCC)


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    Structured Review

    ATCC am2
    Am2, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    am2 - by Bioz Stars, 2022-09
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    ATCC cremoris atcc 19257
    RT-PCR analysis for gadB mRNA. Lanes: 1, L. lactis subsp. lactis biovar diacetylactis 01-7; 2, L. lactis subsp. lactis biovar diacetylactis ATCC 13675; 3, L. lactis subsp. lactis ATCC 19435; 4, L. lactis subsp. lactis 712; 5, L. lactis subsp. lactis SlN; 6, L. lactis subsp. <t>cremoris</t> ATCC 19257; 7, L. lactis subsp. cremoris H-61; 8, L. lactis subsp. cremoris HP.
    Cremoris Atcc 19257, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cremoris atcc 19257/product/ATCC
    Average 94 stars, based on 29 article reviews
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    cremoris atcc 19257 - by Bioz Stars, 2022-09
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    RT-PCR analysis for gadB mRNA. Lanes: 1, L. lactis subsp. lactis biovar diacetylactis 01-7; 2, L. lactis subsp. lactis biovar diacetylactis ATCC 13675; 3, L. lactis subsp. lactis ATCC 19435; 4, L. lactis subsp. lactis 712; 5, L. lactis subsp. lactis SlN; 6, L. lactis subsp. cremoris ATCC 19257; 7, L. lactis subsp. cremoris H-61; 8, L. lactis subsp. cremoris HP.

    Journal: Applied and Environmental Microbiology

    Article Title: Inactivation of the Glutamate Decarboxylase Gene in Lactococcus lactis subsp. cremoris

    doi:

    Figure Lengend Snippet: RT-PCR analysis for gadB mRNA. Lanes: 1, L. lactis subsp. lactis biovar diacetylactis 01-7; 2, L. lactis subsp. lactis biovar diacetylactis ATCC 13675; 3, L. lactis subsp. lactis ATCC 19435; 4, L. lactis subsp. lactis 712; 5, L. lactis subsp. lactis SlN; 6, L. lactis subsp. cremoris ATCC 19257; 7, L. lactis subsp. cremoris H-61; 8, L. lactis subsp. cremoris HP.

    Article Snippet: L. lactis subsp. lactis biovar diacetylactis ATCC 13675, L. lactis subsp. lactis ATCC 19435, and L. lactis subsp. cremoris ATCC 19257 were obtained from the American Type Culture Collection (Manassas, Va.).

    Techniques: Reverse Transcription Polymerase Chain Reaction

    Southern blot analysis of L. lactis gadB . The DNA was digested with Eco RI. The blot was probed with a digoxigenin-labeled 2.4-kb Xba I- Eco ). Lanes: 1, L. lactis subsp. lactis biovar diacetylactis 01-7; 2, L. lactis subsp. lactis biovar diacetylactis ATCC 13675; 3, L. lactis subsp. lactis ATCC 19435; 4, L. lactis subsp. lactis 712; 5, L. lactis subsp. lactis SlN; 6, L. lactis subsp. cremoris ATCC 19257; 7, L. lactis subsp. cremoris H-61; 8, L. lactis subsp. cremoris HP.

    Journal: Applied and Environmental Microbiology

    Article Title: Inactivation of the Glutamate Decarboxylase Gene in Lactococcus lactis subsp. cremoris

    doi:

    Figure Lengend Snippet: Southern blot analysis of L. lactis gadB . The DNA was digested with Eco RI. The blot was probed with a digoxigenin-labeled 2.4-kb Xba I- Eco ). Lanes: 1, L. lactis subsp. lactis biovar diacetylactis 01-7; 2, L. lactis subsp. lactis biovar diacetylactis ATCC 13675; 3, L. lactis subsp. lactis ATCC 19435; 4, L. lactis subsp. lactis 712; 5, L. lactis subsp. lactis SlN; 6, L. lactis subsp. cremoris ATCC 19257; 7, L. lactis subsp. cremoris H-61; 8, L. lactis subsp. cremoris HP.

    Article Snippet: L. lactis subsp. lactis biovar diacetylactis ATCC 13675, L. lactis subsp. lactis ATCC 19435, and L. lactis subsp. cremoris ATCC 19257 were obtained from the American Type Culture Collection (Manassas, Va.).

    Techniques: Southern Blot, Labeling

    Ingestion of Lactococcus Lactis Subsp. cremoris Is Cytoprotective Against Radiological Insult in Mice. A) Survival of 6-week-old C57BL/6 fed the indicated bacteria daily for 3 days followed 12 Gy irradiation insult. Note significantly enhanced survival of only mice treated before irradiation for three days with L. lactis subsp. cremoris ATCC 19257 (Log-Rank test for untreated vs. L. lactis subsp. cremoris treated, P=0.0006, n=5), or with L. rhamnosus GG (Log-Rank test for untreated vs. L. rhamnosus GG P=0.0202, n=5). (B) Weight of mice described in (A) measured daily after irradiation insult. Nonparametric unpaired t-test of bacterial L. lactis subsp. cremoris ATCC 19257 treated against untreated mice. **=P

    Journal: iScience

    Article Title: Lactococcus Lactis Subsp. cremoris Is an Efficacious Beneficial Bacterium that Limits Tissue Injury in the Intestine

    doi: 10.1016/j.isci.2019.01.030

    Figure Lengend Snippet: Ingestion of Lactococcus Lactis Subsp. cremoris Is Cytoprotective Against Radiological Insult in Mice. A) Survival of 6-week-old C57BL/6 fed the indicated bacteria daily for 3 days followed 12 Gy irradiation insult. Note significantly enhanced survival of only mice treated before irradiation for three days with L. lactis subsp. cremoris ATCC 19257 (Log-Rank test for untreated vs. L. lactis subsp. cremoris treated, P=0.0006, n=5), or with L. rhamnosus GG (Log-Rank test for untreated vs. L. rhamnosus GG P=0.0202, n=5). (B) Weight of mice described in (A) measured daily after irradiation insult. Nonparametric unpaired t-test of bacterial L. lactis subsp. cremoris ATCC 19257 treated against untreated mice. **=P

    Article Snippet: Together, our data identify a beneficial bacterium, namely, L . lactis subsp. cremoris ATCC 19257 that exhibits potent anti-inflammatory and cytoprotective effects on the fly and mammalian intestine.

    Techniques: Mouse Assay, Irradiation

    Lactococcus lactis subsp. cremoris ATCC 19257 Protects the Murine Intestine from Dextran Sodium Sulfate (DSS)-Induced Colitis (A) Groups of five 6-week-old male C57/B6 mice were administered either 1 × 10 8 CFU total Lactococcus lactis subsp. cremoris ATCC 19257, or Lactobacillus rhamnosus GG, or vehicle control for 14 days. Feeding of bacteria was ceased before 4% DSS being included in the drinking water (day 0) for 7 days in the indicated groups. Control groups of bacteria-fed mice were subjected to regular water. Measurement of weight loss in mice of the aforementioned groups following commencement of the inclusion of 4% DSS in the drinking water. Data are represented as mean ± SEM. Nonparametric unpaired t test *p

    Journal: iScience

    Article Title: Lactococcus Lactis Subsp. cremoris Is an Efficacious Beneficial Bacterium that Limits Tissue Injury in the Intestine

    doi: 10.1016/j.isci.2019.01.030

    Figure Lengend Snippet: Lactococcus lactis subsp. cremoris ATCC 19257 Protects the Murine Intestine from Dextran Sodium Sulfate (DSS)-Induced Colitis (A) Groups of five 6-week-old male C57/B6 mice were administered either 1 × 10 8 CFU total Lactococcus lactis subsp. cremoris ATCC 19257, or Lactobacillus rhamnosus GG, or vehicle control for 14 days. Feeding of bacteria was ceased before 4% DSS being included in the drinking water (day 0) for 7 days in the indicated groups. Control groups of bacteria-fed mice were subjected to regular water. Measurement of weight loss in mice of the aforementioned groups following commencement of the inclusion of 4% DSS in the drinking water. Data are represented as mean ± SEM. Nonparametric unpaired t test *p

    Article Snippet: Together, our data identify a beneficial bacterium, namely, L . lactis subsp. cremoris ATCC 19257 that exhibits potent anti-inflammatory and cytoprotective effects on the fly and mammalian intestine.

    Techniques: Mouse Assay

    Lactococcus lactis Subsp. Cremoris ATCC 19257 Confers Potent Cytoprotective Effects in the Drosophila Intestine in Response to Oxidative Challenge (A) Graphical representation of experiments conducted to generate data presented in (B–D). (B) Relative survival of germ-free adult Drosophila gnotobiotically mono-colonized with the indicated lactic acid bacteria in response to paraquat challenge. Note only significantly enhanced survival of flies mono-associated with L . lactis subsp. cremoris ATCC 19257 (log rank test for germ-free (g-f) versus g-f + L . lactis subsp. cremoris , p ≤ 0.0006, n = 50), or mono-colonized with Lactobacillus rhamnosus GG (log rank test for g-f versus g-f + L . rhamnosus GG p ≤ 0.0202, n = 50). (C) TUNEL analysis of posterior midgut tissues dissected from Drosophila described in (A) at 24 h following paraquat challenge. Note the presence of TUNEL-positive cells in the Posterior 3 (P3) section of the midgut in response to paraquat. (D) Numeration of TUNEL-positive cells per midgut examined in (C). Data are represented as mean ± SEM. Nonparametric unpaired t test ***p

    Journal: iScience

    Article Title: Lactococcus Lactis Subsp. cremoris Is an Efficacious Beneficial Bacterium that Limits Tissue Injury in the Intestine

    doi: 10.1016/j.isci.2019.01.030

    Figure Lengend Snippet: Lactococcus lactis Subsp. Cremoris ATCC 19257 Confers Potent Cytoprotective Effects in the Drosophila Intestine in Response to Oxidative Challenge (A) Graphical representation of experiments conducted to generate data presented in (B–D). (B) Relative survival of germ-free adult Drosophila gnotobiotically mono-colonized with the indicated lactic acid bacteria in response to paraquat challenge. Note only significantly enhanced survival of flies mono-associated with L . lactis subsp. cremoris ATCC 19257 (log rank test for germ-free (g-f) versus g-f + L . lactis subsp. cremoris , p ≤ 0.0006, n = 50), or mono-colonized with Lactobacillus rhamnosus GG (log rank test for g-f versus g-f + L . rhamnosus GG p ≤ 0.0202, n = 50). (C) TUNEL analysis of posterior midgut tissues dissected from Drosophila described in (A) at 24 h following paraquat challenge. Note the presence of TUNEL-positive cells in the Posterior 3 (P3) section of the midgut in response to paraquat. (D) Numeration of TUNEL-positive cells per midgut examined in (C). Data are represented as mean ± SEM. Nonparametric unpaired t test ***p

    Article Snippet: Together, our data identify a beneficial bacterium, namely, L . lactis subsp. cremoris ATCC 19257 that exhibits potent anti-inflammatory and cytoprotective effects on the fly and mammalian intestine.

    Techniques: TUNEL Assay

    Lactococcus lactis subsp. cremoris ATCC 19257 Induces the Activation of the Nrf2 Signaling Pathway in the Drosophila Intestine by a Mechanism that Does Not Involves the Detectable Generation of Reactive Oxygen Species within Enterocytes (A) Detection of ROS generation following the ingestion of indicated bacteria by germ-free third instar larvae for 1 h. ROS was detected by oxidation of the hydrocyanine ROS-sensitive dye that is present in the larval food. Shown also is the detection of the Nrf2-responsive anti-oxidant response element-dependent GFP expression in the distal midgut of germ-free gstD1- gfp 5-day-old adult Drosophila following ingestion of the indicated bacteria. (B) Detection of transcript enrichment in the midgut of the indicated CncC (Nrf2 in mammals) pathway-responsive genes in germ-free Drosophila mono-associated with the indicated bacteria for 4 h. Data are represented as mean ± SEM. Nonparametric unpaired t test **p

    Journal: iScience

    Article Title: Lactococcus Lactis Subsp. cremoris Is an Efficacious Beneficial Bacterium that Limits Tissue Injury in the Intestine

    doi: 10.1016/j.isci.2019.01.030

    Figure Lengend Snippet: Lactococcus lactis subsp. cremoris ATCC 19257 Induces the Activation of the Nrf2 Signaling Pathway in the Drosophila Intestine by a Mechanism that Does Not Involves the Detectable Generation of Reactive Oxygen Species within Enterocytes (A) Detection of ROS generation following the ingestion of indicated bacteria by germ-free third instar larvae for 1 h. ROS was detected by oxidation of the hydrocyanine ROS-sensitive dye that is present in the larval food. Shown also is the detection of the Nrf2-responsive anti-oxidant response element-dependent GFP expression in the distal midgut of germ-free gstD1- gfp 5-day-old adult Drosophila following ingestion of the indicated bacteria. (B) Detection of transcript enrichment in the midgut of the indicated CncC (Nrf2 in mammals) pathway-responsive genes in germ-free Drosophila mono-associated with the indicated bacteria for 4 h. Data are represented as mean ± SEM. Nonparametric unpaired t test **p

    Article Snippet: Together, our data identify a beneficial bacterium, namely, L . lactis subsp. cremoris ATCC 19257 that exhibits potent anti-inflammatory and cytoprotective effects on the fly and mammalian intestine.

    Techniques: Activation Assay, Expressing

    Multiple alignment of gadB sequences from L. lactis subsp. lactis NIAI 01-7, L. lactis subsp. lactis ATCC 19435, L. lactis subsp. cremoris MG1363, and L. lactis subsp. cremoris ATCC 19257. PCR primers are boxed. Dashed box, Ase I restriction site; dashed arrows, inverted repeat, suggestive of a transcription terminator (not including the poly[T] stretch); asterisks, positions of insertions or deletions. The numbering is according to the sequence of L. lactis subsp. lactis ).

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid PCR-Based Method Which Can Determine Both Phenotype and Genotype of Lactococcus lactis Subspecies

    doi: 10.1128/AEM.68.5.2209-2213.2002

    Figure Lengend Snippet: Multiple alignment of gadB sequences from L. lactis subsp. lactis NIAI 01-7, L. lactis subsp. lactis ATCC 19435, L. lactis subsp. cremoris MG1363, and L. lactis subsp. cremoris ATCC 19257. PCR primers are boxed. Dashed box, Ase I restriction site; dashed arrows, inverted repeat, suggestive of a transcription terminator (not including the poly[T] stretch); asterisks, positions of insertions or deletions. The numbering is according to the sequence of L. lactis subsp. lactis ).

    Article Snippet: L. lactis subsp. lactis strains ATCC 9936 and ATCC 19435, L. lactis subsp. lactis biovar diacetylactis ATCC 13675, L. lactis subsp. cremoris ATCC 19257, Leuconostoc mesenteroides ATCC 8293, and Lactobacillus casei ATCC 393 were obtained from the American Type Culture Collection (Manassas, Va.).

    Techniques: Polymerase Chain Reaction, Sequencing

    PCR amplification and digestion with Ase I of the gadB gene in isolates from cheese starters. Conditions for PCR and electrophoresis are described in Materials and Methods. Lanes: 1, L. lactis subsp. lactis biovar diacetylactis NIAI 01-7; 2, L. lactis subsp. lactis ATCC 19435; 3, L. lactis subsp. cremoris ATCC 19257; 4, L. lactis subsp. cremoris 53-2; 5, L. lactis subsp. cremoris 53-6.

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid PCR-Based Method Which Can Determine Both Phenotype and Genotype of Lactococcus lactis Subspecies

    doi: 10.1128/AEM.68.5.2209-2213.2002

    Figure Lengend Snippet: PCR amplification and digestion with Ase I of the gadB gene in isolates from cheese starters. Conditions for PCR and electrophoresis are described in Materials and Methods. Lanes: 1, L. lactis subsp. lactis biovar diacetylactis NIAI 01-7; 2, L. lactis subsp. lactis ATCC 19435; 3, L. lactis subsp. cremoris ATCC 19257; 4, L. lactis subsp. cremoris 53-2; 5, L. lactis subsp. cremoris 53-6.

    Article Snippet: L. lactis subsp. lactis strains ATCC 9936 and ATCC 19435, L. lactis subsp. lactis biovar diacetylactis ATCC 13675, L. lactis subsp. cremoris ATCC 19257, Leuconostoc mesenteroides ATCC 8293, and Lactobacillus casei ATCC 393 were obtained from the American Type Culture Collection (Manassas, Va.).

    Techniques: Polymerase Chain Reaction, Amplification, Electrophoresis