Journal: Applied and Environmental Microbiology
Article Title: Rapid PCR-Based Method Which Can Determine Both Phenotype and Genotype of Lactococcus lactis Subspecies
Figure Lengend Snippet: Multiple alignment of gadB sequences from L. lactis subsp. lactis NIAI 01-7, L. lactis subsp. lactis ATCC 19435, L. lactis subsp. cremoris MG1363, and L. lactis subsp. cremoris ATCC 19257. PCR primers are boxed. Dashed box, Ase I restriction site; dashed arrows, inverted repeat, suggestive of a transcription terminator (not including the poly[T] stretch); asterisks, positions of insertions or deletions. The numbering is according to the sequence of L. lactis subsp. lactis ).
Article Snippet: L. lactis subsp. lactis strains ATCC 9936 and ATCC 19435, L. lactis subsp. lactis biovar diacetylactis ATCC 13675, L. lactis subsp. cremoris ATCC 19257, Leuconostoc mesenteroides ATCC 8293, and Lactobacillus casei ATCC 393 were obtained from the American Type Culture Collection (Manassas, Va.).
Techniques: Polymerase Chain Reaction, Sequencing