nsp7 97 096  (ProSci Incorporated)


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    Structured Review

    ProSci Incorporated nsp7 97 096
    SARS-CoV2 nucleocapsid protein (NP) is a potent inducer of human endothelial cell activation. (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins; 1 μg/ml) and five nonstructural proteins (NSP1, NSP3, NSP5, <t>NSP7,</t> and NSP8; 1 μg/ml) for 8 h. (b) HLMECs were treated with 1 μg/ml of NP or 10 ng/ml of TNF-α for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 h. TNF-α at 10 ng/ml served as a positive control. (d) Different cultured cells, including mouse lung vascular endothelial cells (MECs), A549 cells, 293T cells, HUVECs, HAECs, HCAECs, HDMECs, and HLMECs, were treated with NP (1 μg/ml) for 8 h. The expression of ICAM-1, VCAM-1, and VE-cadherin was detected by Western blotting. β-Actin served as a loading control. (e) HLMECs were treated with PBS, NP (1 μg/ml), TNF-α (10 ng/ml), or lipopolysaccharide (LPS) (1 μg/ml) for 8 h. The total RNA was isolated and qPCR was performed for measuring the mRNA levels of TNF-α, ICAM-1, VCAM-1, MCP-1, and IL-6. (f) HLMECs were treated with PBS, NP (1 μg/ml), or TNF-α (10 ng/ml) for 8 h and cocultured with Zombie Red-labeled THP-1 cells for 1 h. After being washed, the adherent cells were imaged and quantitatively analyzed.
    Nsp7 97 096, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nsp7 97 096/product/ProSci Incorporated
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nsp7 97 096 - by Bioz Stars, 2023-12
    92/100 stars

    Images

    1) Product Images from "Direct Activation of Endothelial Cells by SARS-CoV-2 Nucleocapsid Protein Is Blocked by Simvastatin"

    Article Title: Direct Activation of Endothelial Cells by SARS-CoV-2 Nucleocapsid Protein Is Blocked by Simvastatin

    Journal: Journal of Virology

    doi: 10.1128/JVI.01396-21

    SARS-CoV2 nucleocapsid protein (NP) is a potent inducer of human endothelial cell activation. (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins; 1 μg/ml) and five nonstructural proteins (NSP1, NSP3, NSP5, NSP7, and NSP8; 1 μg/ml) for 8 h. (b) HLMECs were treated with 1 μg/ml of NP or 10 ng/ml of TNF-α for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 h. TNF-α at 10 ng/ml served as a positive control. (d) Different cultured cells, including mouse lung vascular endothelial cells (MECs), A549 cells, 293T cells, HUVECs, HAECs, HCAECs, HDMECs, and HLMECs, were treated with NP (1 μg/ml) for 8 h. The expression of ICAM-1, VCAM-1, and VE-cadherin was detected by Western blotting. β-Actin served as a loading control. (e) HLMECs were treated with PBS, NP (1 μg/ml), TNF-α (10 ng/ml), or lipopolysaccharide (LPS) (1 μg/ml) for 8 h. The total RNA was isolated and qPCR was performed for measuring the mRNA levels of TNF-α, ICAM-1, VCAM-1, MCP-1, and IL-6. (f) HLMECs were treated with PBS, NP (1 μg/ml), or TNF-α (10 ng/ml) for 8 h and cocultured with Zombie Red-labeled THP-1 cells for 1 h. After being washed, the adherent cells were imaged and quantitatively analyzed.
    Figure Legend Snippet: SARS-CoV2 nucleocapsid protein (NP) is a potent inducer of human endothelial cell activation. (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins; 1 μg/ml) and five nonstructural proteins (NSP1, NSP3, NSP5, NSP7, and NSP8; 1 μg/ml) for 8 h. (b) HLMECs were treated with 1 μg/ml of NP or 10 ng/ml of TNF-α for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 h. TNF-α at 10 ng/ml served as a positive control. (d) Different cultured cells, including mouse lung vascular endothelial cells (MECs), A549 cells, 293T cells, HUVECs, HAECs, HCAECs, HDMECs, and HLMECs, were treated with NP (1 μg/ml) for 8 h. The expression of ICAM-1, VCAM-1, and VE-cadherin was detected by Western blotting. β-Actin served as a loading control. (e) HLMECs were treated with PBS, NP (1 μg/ml), TNF-α (10 ng/ml), or lipopolysaccharide (LPS) (1 μg/ml) for 8 h. The total RNA was isolated and qPCR was performed for measuring the mRNA levels of TNF-α, ICAM-1, VCAM-1, MCP-1, and IL-6. (f) HLMECs were treated with PBS, NP (1 μg/ml), or TNF-α (10 ng/ml) for 8 h and cocultured with Zombie Red-labeled THP-1 cells for 1 h. After being washed, the adherent cells were imaged and quantitatively analyzed.

    Techniques Used: Activation Assay, Incubation, Positive Control, Cell Culture, Expressing, Western Blot, Isolation, Labeling

    nsp7 97 096  (ProSci Incorporated)


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    Structured Review

    ProSci Incorporated nsp7 97 096
    SARS-CoV2 nucleocapsid protein (NP) is a potent inducer of human endothelial cell activation. (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins; 1 μg/ml) and five nonstructural proteins (NSP1, NSP3, NSP5, <t>NSP7,</t> and NSP8; 1 μg/ml) for 8 h. (b) HLMECs were treated with 1 μg/ml of NP or 10 ng/ml of TNF-α for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 h. TNF-α at 10 ng/ml served as a positive control. (d) Different cultured cells, including mouse lung vascular endothelial cells (MECs), A549 cells, 293T cells, HUVECs, HAECs, HCAECs, HDMECs, and HLMECs, were treated with NP (1 μg/ml) for 8 h. The expression of ICAM-1, VCAM-1, and VE-cadherin was detected by Western blotting. β-Actin served as a loading control. (e) HLMECs were treated with PBS, NP (1 μg/ml), TNF-α (10 ng/ml), or lipopolysaccharide (LPS) (1 μg/ml) for 8 h. The total RNA was isolated and qPCR was performed for measuring the mRNA levels of TNF-α, ICAM-1, VCAM-1, MCP-1, and IL-6. (f) HLMECs were treated with PBS, NP (1 μg/ml), or TNF-α (10 ng/ml) for 8 h and cocultured with Zombie Red-labeled THP-1 cells for 1 h. After being washed, the adherent cells were imaged and quantitatively analyzed.
    Nsp7 97 096, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nsp7 97 096/product/ProSci Incorporated
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nsp7 97 096 - by Bioz Stars, 2023-12
    92/100 stars

    Images

    1) Product Images from "Direct Activation of Endothelial Cells by SARS-CoV-2 Nucleocapsid Protein Is Blocked by Simvastatin"

    Article Title: Direct Activation of Endothelial Cells by SARS-CoV-2 Nucleocapsid Protein Is Blocked by Simvastatin

    Journal: Journal of Virology

    doi: 10.1128/JVI.01396-21

    SARS-CoV2 nucleocapsid protein (NP) is a potent inducer of human endothelial cell activation. (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins; 1 μg/ml) and five nonstructural proteins (NSP1, NSP3, NSP5, NSP7, and NSP8; 1 μg/ml) for 8 h. (b) HLMECs were treated with 1 μg/ml of NP or 10 ng/ml of TNF-α for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 h. TNF-α at 10 ng/ml served as a positive control. (d) Different cultured cells, including mouse lung vascular endothelial cells (MECs), A549 cells, 293T cells, HUVECs, HAECs, HCAECs, HDMECs, and HLMECs, were treated with NP (1 μg/ml) for 8 h. The expression of ICAM-1, VCAM-1, and VE-cadherin was detected by Western blotting. β-Actin served as a loading control. (e) HLMECs were treated with PBS, NP (1 μg/ml), TNF-α (10 ng/ml), or lipopolysaccharide (LPS) (1 μg/ml) for 8 h. The total RNA was isolated and qPCR was performed for measuring the mRNA levels of TNF-α, ICAM-1, VCAM-1, MCP-1, and IL-6. (f) HLMECs were treated with PBS, NP (1 μg/ml), or TNF-α (10 ng/ml) for 8 h and cocultured with Zombie Red-labeled THP-1 cells for 1 h. After being washed, the adherent cells were imaged and quantitatively analyzed.
    Figure Legend Snippet: SARS-CoV2 nucleocapsid protein (NP) is a potent inducer of human endothelial cell activation. (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins; 1 μg/ml) and five nonstructural proteins (NSP1, NSP3, NSP5, NSP7, and NSP8; 1 μg/ml) for 8 h. (b) HLMECs were treated with 1 μg/ml of NP or 10 ng/ml of TNF-α for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 h. TNF-α at 10 ng/ml served as a positive control. (d) Different cultured cells, including mouse lung vascular endothelial cells (MECs), A549 cells, 293T cells, HUVECs, HAECs, HCAECs, HDMECs, and HLMECs, were treated with NP (1 μg/ml) for 8 h. The expression of ICAM-1, VCAM-1, and VE-cadherin was detected by Western blotting. β-Actin served as a loading control. (e) HLMECs were treated with PBS, NP (1 μg/ml), TNF-α (10 ng/ml), or lipopolysaccharide (LPS) (1 μg/ml) for 8 h. The total RNA was isolated and qPCR was performed for measuring the mRNA levels of TNF-α, ICAM-1, VCAM-1, MCP-1, and IL-6. (f) HLMECs were treated with PBS, NP (1 μg/ml), or TNF-α (10 ng/ml) for 8 h and cocultured with Zombie Red-labeled THP-1 cells for 1 h. After being washed, the adherent cells were imaged and quantitatively analyzed.

    Techniques Used: Activation Assay, Incubation, Positive Control, Cell Culture, Expressing, Western Blot, Isolation, Labeling

    nsp7 97 096  (ProSci Incorporated)


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    Structured Review

    ProSci Incorporated nsp7 97 096
    (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins, 1 μg/mL) and five non-structural proteins (NSP1, NSP3, NSP5, <t>NSP7</t> and NSP8, 1 μg/mL) for 8 hrs. (b) HLMECs were treated with 1 μg/mL of NP or 10 ng/ml of TNFα for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 hrs. 10 ng/ml of TNFα serves as positive control. (d) Different cultured cells including mouse lung vascular endothelial cells (MEC), A549, 293T, HUVEC, HAEC, HCAEC, HDMEC and HLMEC were treated with NP (1 μg/mL) for 8 hrs. The expression of ICAM-1, VCAM-1 and VE-cadherin was detected by western blot. β-actin was served as loading control. (e) HLMECs were treated with PBS, NP (1 μg/ml), TNFα (10 ng/ml) or LPS (1 μg/ml) for 8 hours. The total RNA was isolated and QPCR was performed for measuring the mRNA levels of TNFα, ICAM-1, VCAM-1, MCP-1 and IL-6. (f) HLMECs were treated with PBS, NP (1 μg/ml) or TNFα (10 ng/ml) for 8 hours and co-cultured with Zombie Red-labeled THP-1 cells for 1h. After washing, the adherent cells were imaged and quantitatively analyzed.
    Nsp7 97 096, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nsp7 97 096/product/ProSci Incorporated
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nsp7 97 096 - by Bioz Stars, 2023-12
    92/100 stars

    Images

    1) Product Images from "Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin"

    Article Title: Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin

    Journal: bioRxiv

    doi: 10.1101/2021.02.14.431174

    (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins, 1 μg/mL) and five non-structural proteins (NSP1, NSP3, NSP5, NSP7 and NSP8, 1 μg/mL) for 8 hrs. (b) HLMECs were treated with 1 μg/mL of NP or 10 ng/ml of TNFα for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 hrs. 10 ng/ml of TNFα serves as positive control. (d) Different cultured cells including mouse lung vascular endothelial cells (MEC), A549, 293T, HUVEC, HAEC, HCAEC, HDMEC and HLMEC were treated with NP (1 μg/mL) for 8 hrs. The expression of ICAM-1, VCAM-1 and VE-cadherin was detected by western blot. β-actin was served as loading control. (e) HLMECs were treated with PBS, NP (1 μg/ml), TNFα (10 ng/ml) or LPS (1 μg/ml) for 8 hours. The total RNA was isolated and QPCR was performed for measuring the mRNA levels of TNFα, ICAM-1, VCAM-1, MCP-1 and IL-6. (f) HLMECs were treated with PBS, NP (1 μg/ml) or TNFα (10 ng/ml) for 8 hours and co-cultured with Zombie Red-labeled THP-1 cells for 1h. After washing, the adherent cells were imaged and quantitatively analyzed.
    Figure Legend Snippet: (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins, 1 μg/mL) and five non-structural proteins (NSP1, NSP3, NSP5, NSP7 and NSP8, 1 μg/mL) for 8 hrs. (b) HLMECs were treated with 1 μg/mL of NP or 10 ng/ml of TNFα for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 hrs. 10 ng/ml of TNFα serves as positive control. (d) Different cultured cells including mouse lung vascular endothelial cells (MEC), A549, 293T, HUVEC, HAEC, HCAEC, HDMEC and HLMEC were treated with NP (1 μg/mL) for 8 hrs. The expression of ICAM-1, VCAM-1 and VE-cadherin was detected by western blot. β-actin was served as loading control. (e) HLMECs were treated with PBS, NP (1 μg/ml), TNFα (10 ng/ml) or LPS (1 μg/ml) for 8 hours. The total RNA was isolated and QPCR was performed for measuring the mRNA levels of TNFα, ICAM-1, VCAM-1, MCP-1 and IL-6. (f) HLMECs were treated with PBS, NP (1 μg/ml) or TNFα (10 ng/ml) for 8 hours and co-cultured with Zombie Red-labeled THP-1 cells for 1h. After washing, the adherent cells were imaged and quantitatively analyzed.

    Techniques Used: Incubation, Positive Control, Cell Culture, Expressing, Western Blot, Isolation, Labeling

    nsp7 97 096  (ProSci Incorporated)


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    Structured Review

    ProSci Incorporated nsp7 97 096
    (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins, 1 μg/mL) and five non-structural proteins (NSP1, NSP3, NSP5, <t>NSP7</t> and NSP8, 1 μg/mL) for 8 hrs. (b) HLMECs were treated with 1 μg/mL of NP or 10 ng/ml of TNFα for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 hrs. 10 ng/ml of TNFα serves as positive control. (d) Different cultured cells including mouse lung vascular endothelial cells (MEC), A549, 293T, HUVEC, HAEC, HCAEC, HDMEC and HLMEC were treated with NP (1 μg/mL) for 8 hrs. The expression of ICAM-1, VCAM-1 and VE-cadherin was detected by western blot. β-actin was served as loading control. (e) HLMECs were treated with PBS, NP (1 µg/ml), TNFα (10 ng/ml) or LPS (1 µg/ml) for 8 hours. The total RNA was isolated and QPCR was performed for measuring the mRNA levels of TNFα, ICAM-1, VCAM-1, MCP-1 and IL-6. (f) HLMECs were treated with PBS, NP (1 µg/ml) or TNFα (10 ng/ml) for 8 hours and co-cultured with Zombie Red-labeled THP-1 cells for 1h. After washing, the adherent cells were imaged and quantitatively analyzed.
    Nsp7 97 096, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nsp7 97 096/product/ProSci Incorporated
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nsp7 97 096 - by Bioz Stars, 2023-12
    92/100 stars

    Images

    1) Product Images from "Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin"

    Article Title: Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin

    Journal: bioRxiv

    doi: 10.1101/2021.02.14.431174

    (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins, 1 μg/mL) and five non-structural proteins (NSP1, NSP3, NSP5, NSP7 and NSP8, 1 μg/mL) for 8 hrs. (b) HLMECs were treated with 1 μg/mL of NP or 10 ng/ml of TNFα for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 hrs. 10 ng/ml of TNFα serves as positive control. (d) Different cultured cells including mouse lung vascular endothelial cells (MEC), A549, 293T, HUVEC, HAEC, HCAEC, HDMEC and HLMEC were treated with NP (1 μg/mL) for 8 hrs. The expression of ICAM-1, VCAM-1 and VE-cadherin was detected by western blot. β-actin was served as loading control. (e) HLMECs were treated with PBS, NP (1 µg/ml), TNFα (10 ng/ml) or LPS (1 µg/ml) for 8 hours. The total RNA was isolated and QPCR was performed for measuring the mRNA levels of TNFα, ICAM-1, VCAM-1, MCP-1 and IL-6. (f) HLMECs were treated with PBS, NP (1 µg/ml) or TNFα (10 ng/ml) for 8 hours and co-cultured with Zombie Red-labeled THP-1 cells for 1h. After washing, the adherent cells were imaged and quantitatively analyzed.
    Figure Legend Snippet: (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins, 1 μg/mL) and five non-structural proteins (NSP1, NSP3, NSP5, NSP7 and NSP8, 1 μg/mL) for 8 hrs. (b) HLMECs were treated with 1 μg/mL of NP or 10 ng/ml of TNFα for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 hrs. 10 ng/ml of TNFα serves as positive control. (d) Different cultured cells including mouse lung vascular endothelial cells (MEC), A549, 293T, HUVEC, HAEC, HCAEC, HDMEC and HLMEC were treated with NP (1 μg/mL) for 8 hrs. The expression of ICAM-1, VCAM-1 and VE-cadherin was detected by western blot. β-actin was served as loading control. (e) HLMECs were treated with PBS, NP (1 µg/ml), TNFα (10 ng/ml) or LPS (1 µg/ml) for 8 hours. The total RNA was isolated and QPCR was performed for measuring the mRNA levels of TNFα, ICAM-1, VCAM-1, MCP-1 and IL-6. (f) HLMECs were treated with PBS, NP (1 µg/ml) or TNFα (10 ng/ml) for 8 hours and co-cultured with Zombie Red-labeled THP-1 cells for 1h. After washing, the adherent cells were imaged and quantitatively analyzed.

    Techniques Used: Incubation, Positive Control, Cell Culture, Expressing, Western Blot, Isolation, Labeling

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    ProSci Incorporated nsp7 97 096
    SARS-CoV2 nucleocapsid protein (NP) is a potent inducer of human endothelial cell activation. (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins; 1 μg/ml) and five nonstructural proteins (NSP1, NSP3, NSP5, <t>NSP7,</t> and NSP8; 1 μg/ml) for 8 h. (b) HLMECs were treated with 1 μg/ml of NP or 10 ng/ml of TNF-α for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 h. TNF-α at 10 ng/ml served as a positive control. (d) Different cultured cells, including mouse lung vascular endothelial cells (MECs), A549 cells, 293T cells, HUVECs, HAECs, HCAECs, HDMECs, and HLMECs, were treated with NP (1 μg/ml) for 8 h. The expression of ICAM-1, VCAM-1, and VE-cadherin was detected by Western blotting. β-Actin served as a loading control. (e) HLMECs were treated with PBS, NP (1 μg/ml), TNF-α (10 ng/ml), or lipopolysaccharide (LPS) (1 μg/ml) for 8 h. The total RNA was isolated and qPCR was performed for measuring the mRNA levels of TNF-α, ICAM-1, VCAM-1, MCP-1, and IL-6. (f) HLMECs were treated with PBS, NP (1 μg/ml), or TNF-α (10 ng/ml) for 8 h and cocultured with Zombie Red-labeled THP-1 cells for 1 h. After being washed, the adherent cells were imaged and quantitatively analyzed.
    Nsp7 97 096, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nsp7 97 096/product/ProSci Incorporated
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nsp7 97 096 - by Bioz Stars, 2023-12
    92/100 stars
    		  Buy from Supplier

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    SARS-CoV2 nucleocapsid protein (NP) is a potent inducer of human endothelial cell activation. (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins; 1 μg/ml) and five nonstructural proteins (NSP1, NSP3, NSP5, NSP7, and NSP8; 1 μg/ml) for 8 h. (b) HLMECs were treated with 1 μg/ml of NP or 10 ng/ml of TNF-α for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 h. TNF-α at 10 ng/ml served as a positive control. (d) Different cultured cells, including mouse lung vascular endothelial cells (MECs), A549 cells, 293T cells, HUVECs, HAECs, HCAECs, HDMECs, and HLMECs, were treated with NP (1 μg/ml) for 8 h. The expression of ICAM-1, VCAM-1, and VE-cadherin was detected by Western blotting. β-Actin served as a loading control. (e) HLMECs were treated with PBS, NP (1 μg/ml), TNF-α (10 ng/ml), or lipopolysaccharide (LPS) (1 μg/ml) for 8 h. The total RNA was isolated and qPCR was performed for measuring the mRNA levels of TNF-α, ICAM-1, VCAM-1, MCP-1, and IL-6. (f) HLMECs were treated with PBS, NP (1 μg/ml), or TNF-α (10 ng/ml) for 8 h and cocultured with Zombie Red-labeled THP-1 cells for 1 h. After being washed, the adherent cells were imaged and quantitatively analyzed.

    Journal: Journal of Virology

    Article Title: Direct Activation of Endothelial Cells by SARS-CoV-2 Nucleocapsid Protein Is Blocked by Simvastatin

    doi: 10.1128/JVI.01396-21

    Figure Lengend Snippet: SARS-CoV2 nucleocapsid protein (NP) is a potent inducer of human endothelial cell activation. (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins; 1 μg/ml) and five nonstructural proteins (NSP1, NSP3, NSP5, NSP7, and NSP8; 1 μg/ml) for 8 h. (b) HLMECs were treated with 1 μg/ml of NP or 10 ng/ml of TNF-α for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 h. TNF-α at 10 ng/ml served as a positive control. (d) Different cultured cells, including mouse lung vascular endothelial cells (MECs), A549 cells, 293T cells, HUVECs, HAECs, HCAECs, HDMECs, and HLMECs, were treated with NP (1 μg/ml) for 8 h. The expression of ICAM-1, VCAM-1, and VE-cadherin was detected by Western blotting. β-Actin served as a loading control. (e) HLMECs were treated with PBS, NP (1 μg/ml), TNF-α (10 ng/ml), or lipopolysaccharide (LPS) (1 μg/ml) for 8 h. The total RNA was isolated and qPCR was performed for measuring the mRNA levels of TNF-α, ICAM-1, VCAM-1, MCP-1, and IL-6. (f) HLMECs were treated with PBS, NP (1 μg/ml), or TNF-α (10 ng/ml) for 8 h and cocultured with Zombie Red-labeled THP-1 cells for 1 h. After being washed, the adherent cells were imaged and quantitatively analyzed.

    Article Snippet: SARS-CoV-2 NSP1 (97-095), NSP5 (10-116), NSP7 (97-096), and NSP8 (97-097) proteins were obtained from Prosci (Poway, CA).

    Techniques: Activation Assay, Incubation, Positive Control, Cell Culture, Expressing, Western Blot, Isolation, Labeling