Journal: Science Advances

Article Title: Synthetic gene circuits for preventing disruption of the circadian clock due to interleukin-1–induced inflammation

doi: 10.1126/sciadv.abj8892

Figure Lengend Snippet: ( A ) Differentiation protocol of miPSCs to chondrocytes. miPSCs were subjected to high-density micromass culture to create PDiPSCs. PDiPSCs were then either cast in agarose or pelleted and cultured in chondrogenic media for 14 to 21 days to create tissue-engineered cartilage. ( B ) P2L bioluminescence intensity (BLI) of miPSCs ( n = 10), PDiPSCs ( n = 2), PDiPSCs in agarose ( n = 4), and pellets ( n = 10) (left) and B1L bioluminescence intensity (BLI) of miPSCs ( n = 5), PDiPSCs ( n = 4), PDiPSCs in agarose ( n = 5) (middle), and pellets ( n = 5) (right). ( C ) BLI of femoral head cartilage explants from PER2::LUC mice ( n = 3, one hip per mouse). ( D ) P2L BLI in porcine chondrocytes cast in agarose ( n = 5). Shaded region on graphs represents SEM.

Article Snippet: Two luciferase reporter lentiviral vectors, one driven by the murine Bmal1 promoter (Addgene no. 182761) and one driven by the murine Period 2 (Per2) promoter (Addgene no. 182762), were used ( ).

Techniques: Cell Culture

Journal: Science Advances

Article Title: Synthetic gene circuits for preventing disruption of the circadian clock due to interleukin-1–induced inflammation

doi: 10.1126/sciadv.abj8892

Figure Lengend Snippet: Per2 expression in cartilage explants from the femoral head of PER2::LUC mice was recorded as bioluminescence intensity. Explants were treated with IL-1β (1 ng/ml), IL-1⍺ (1 ng/ml), TNF⍺ (20 ng/ml), or vehicle controls ( n = 3 per each condition). Loss of circadian rhythm was seen in explants, given IL-1 and circadian rhythm was rescued with the administration of dex. Gray, blue, orange, and red bars indicate administration of PBS or cytokine; black bars indicate administration of dex. Shaded region on graph represents SEM. Asterisks represent significance compared to controls ( P < 0.05). ** P < 0.01; *** P < 0.05.

Article Snippet: Two luciferase reporter lentiviral vectors, one driven by the murine Bmal1 promoter (Addgene no. 182761) and one driven by the murine Period 2 (Per2) promoter (Addgene no. 182762), were used ( ).

Techniques: Expressing