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Western blot analysis for tyrosine hydroxylase and α-synuclein proteins. (A) Western blot gels for the selected markers. (B, C) Bar charts indicating the density of proteins. Data plotted represent the mean ± SD; one-way ANOVA and Bonferroni tests were applied with a threshold of p < 0.05 ( n = 5). Symbols indicate significant differences compared to: (*) the vehicle group, or (#) the PD control group.

Journal: Frontiers in Pharmacology

Article Title: Marula oil nanoemulsion improves motor function in experimental parkinsonism via mitigation of inflammation and oxidative stress

doi: 10.3389/fphar.2023.1293306

Figure Lengend Snippet: Western blot analysis for tyrosine hydroxylase and α-synuclein proteins. (A) Western blot gels for the selected markers. (B, C) Bar charts indicating the density of proteins. Data plotted represent the mean ± SD; one-way ANOVA and Bonferroni tests were applied with a threshold of p < 0.05 ( n = 5). Symbols indicate significant differences compared to: (*) the vehicle group, or (#) the PD control group.

Article Snippet: Primary antibodies against tyrosine hydroxylase (1:1000, PA1-4679, Thermo Fisher, USA), alpha-synuclein (1:500, PA1-18264, Thermo Fisher, USA), and β-actin (1:2000, PA1-183, Thermo Fisher, USA) were incubated overnight at 4°C on the membranes.

Techniques: Western Blot, Control