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rat cd24  (ProSci Incorporated)


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    Structured Review

    ProSci Incorporated rat cd24
    Characterization of NP structures by transmission electron microscopy (TEM) and immunohistochemistry. (A) Transmission electron micrographs depict vacuole structures inside NP cells (labelled with white V) and immunofluorescence staining of NP cells show expression for lysosomal marker Lamp1. Lamp 1 is highly expressed in NP with presence of large vacuolar structures (IF and transmission electron microscopy) These structures resent with heterogenous staining for lysosomal marker Lamp 1 indicating potential existence of different types of vacuoles. (B) Microscopy images of live tail NP cells labelled with LysoTracker for 20 min (a) Hoescht, (b) LysoTracker and (c) differential interference contrast DIC and fixed tail NP cells labelled with LipidTOX green neutral lipid stain (d) Hoescht (e) LipidTOX and (f) DIC. (C) Immunofluorescence staining of IVD shows high expression of Slc12a2 in NP. (D) TEM of NP cells showing structures involved in cell–cell communication (a) shows presence of desmosome-like structures (b) as well as gap junctions (c & d, white arrows). (E) Immunofluorescence shows expression of Cd81 and Cd109 in NP. FACS analysis shows that Cd81 and Cd109 have high co-expression with NP marker <t>Cd24.</t> (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    Rat Cd24, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "PRIMUS: Comprehensive proteomics of mouse intervertebral discs that inform novel biology and relevance to human disease modelling"

    Article Title: PRIMUS: Comprehensive proteomics of mouse intervertebral discs that inform novel biology and relevance to human disease modelling

    Journal: Matrix Biology Plus

    doi: 10.1016/j.mbplus.2021.100082

    Characterization of NP structures by transmission electron microscopy (TEM) and immunohistochemistry. (A) Transmission electron micrographs depict vacuole structures inside NP cells (labelled with white V) and immunofluorescence staining of NP cells show expression for lysosomal marker Lamp1. Lamp 1 is highly expressed in NP with presence of large vacuolar structures (IF and transmission electron microscopy) These structures resent with heterogenous staining for lysosomal marker Lamp 1 indicating potential existence of different types of vacuoles. (B) Microscopy images of live tail NP cells labelled with LysoTracker for 20 min (a) Hoescht, (b) LysoTracker and (c) differential interference contrast DIC and fixed tail NP cells labelled with LipidTOX green neutral lipid stain (d) Hoescht (e) LipidTOX and (f) DIC. (C) Immunofluorescence staining of IVD shows high expression of Slc12a2 in NP. (D) TEM of NP cells showing structures involved in cell–cell communication (a) shows presence of desmosome-like structures (b) as well as gap junctions (c & d, white arrows). (E) Immunofluorescence shows expression of Cd81 and Cd109 in NP. FACS analysis shows that Cd81 and Cd109 have high co-expression with NP marker Cd24. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Characterization of NP structures by transmission electron microscopy (TEM) and immunohistochemistry. (A) Transmission electron micrographs depict vacuole structures inside NP cells (labelled with white V) and immunofluorescence staining of NP cells show expression for lysosomal marker Lamp1. Lamp 1 is highly expressed in NP with presence of large vacuolar structures (IF and transmission electron microscopy) These structures resent with heterogenous staining for lysosomal marker Lamp 1 indicating potential existence of different types of vacuoles. (B) Microscopy images of live tail NP cells labelled with LysoTracker for 20 min (a) Hoescht, (b) LysoTracker and (c) differential interference contrast DIC and fixed tail NP cells labelled with LipidTOX green neutral lipid stain (d) Hoescht (e) LipidTOX and (f) DIC. (C) Immunofluorescence staining of IVD shows high expression of Slc12a2 in NP. (D) TEM of NP cells showing structures involved in cell–cell communication (a) shows presence of desmosome-like structures (b) as well as gap junctions (c & d, white arrows). (E) Immunofluorescence shows expression of Cd81 and Cd109 in NP. FACS analysis shows that Cd81 and Cd109 have high co-expression with NP marker Cd24. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Transmission Assay, Electron Microscopy, Immunohistochemistry, Immunofluorescence, Staining, Expressing, Marker, Microscopy



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    ProSci Incorporated rat cd24
    Characterization of NP structures by transmission electron microscopy (TEM) and immunohistochemistry. (A) Transmission electron micrographs depict vacuole structures inside NP cells (labelled with white V) and immunofluorescence staining of NP cells show expression for lysosomal marker Lamp1. Lamp 1 is highly expressed in NP with presence of large vacuolar structures (IF and transmission electron microscopy) These structures resent with heterogenous staining for lysosomal marker Lamp 1 indicating potential existence of different types of vacuoles. (B) Microscopy images of live tail NP cells labelled with LysoTracker for 20 min (a) Hoescht, (b) LysoTracker and (c) differential interference contrast DIC and fixed tail NP cells labelled with LipidTOX green neutral lipid stain (d) Hoescht (e) LipidTOX and (f) DIC. (C) Immunofluorescence staining of IVD shows high expression of Slc12a2 in NP. (D) TEM of NP cells showing structures involved in cell–cell communication (a) shows presence of desmosome-like structures (b) as well as gap junctions (c & d, white arrows). (E) Immunofluorescence shows expression of Cd81 and Cd109 in NP. FACS analysis shows that Cd81 and Cd109 have high co-expression with NP marker <t>Cd24.</t> (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
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    Characterization of NP structures by transmission electron microscopy (TEM) and immunohistochemistry. (A) Transmission electron micrographs depict vacuole structures inside NP cells (labelled with white V) and immunofluorescence staining of NP cells show expression for lysosomal marker Lamp1. Lamp 1 is highly expressed in NP with presence of large vacuolar structures (IF and transmission electron microscopy) These structures resent with heterogenous staining for lysosomal marker Lamp 1 indicating potential existence of different types of vacuoles. (B) Microscopy images of live tail NP cells labelled with LysoTracker for 20 min (a) Hoescht, (b) LysoTracker and (c) differential interference contrast DIC and fixed tail NP cells labelled with LipidTOX green neutral lipid stain (d) Hoescht (e) LipidTOX and (f) DIC. (C) Immunofluorescence staining of IVD shows high expression of Slc12a2 in NP. (D) TEM of NP cells showing structures involved in cell–cell communication (a) shows presence of desmosome-like structures (b) as well as gap junctions (c & d, white arrows). (E) Immunofluorescence shows expression of Cd81 and Cd109 in NP. FACS analysis shows that Cd81 and Cd109 have high co-expression with NP marker <t>Cd24.</t> (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
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    Characterization of NP structures by transmission electron microscopy (TEM) and immunohistochemistry. (A) Transmission electron micrographs depict vacuole structures inside NP cells (labelled with white V) and immunofluorescence staining of NP cells show expression for lysosomal marker Lamp1. Lamp 1 is highly expressed in NP with presence of large vacuolar structures (IF and transmission electron microscopy) These structures resent with heterogenous staining for lysosomal marker Lamp 1 indicating potential existence of different types of vacuoles. (B) Microscopy images of live tail NP cells labelled with LysoTracker for 20 min (a) Hoescht, (b) LysoTracker and (c) differential interference contrast DIC and fixed tail NP cells labelled with LipidTOX green neutral lipid stain (d) Hoescht (e) LipidTOX and (f) DIC. (C) Immunofluorescence staining of IVD shows high expression of Slc12a2 in NP. (D) TEM of NP cells showing structures involved in cell–cell communication (a) shows presence of desmosome-like structures (b) as well as gap junctions (c & d, white arrows). (E) Immunofluorescence shows expression of Cd81 and Cd109 in NP. FACS analysis shows that Cd81 and Cd109 have high co-expression with NP marker <t>Cd24.</t> (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
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    Characterization of NP structures by transmission electron microscopy (TEM) and immunohistochemistry. (A) Transmission electron micrographs depict vacuole structures inside NP cells (labelled with white V) and immunofluorescence staining of NP cells show expression for lysosomal marker Lamp1. Lamp 1 is highly expressed in NP with presence of large vacuolar structures (IF and transmission electron microscopy) These structures resent with heterogenous staining for lysosomal marker Lamp 1 indicating potential existence of different types of vacuoles. (B) Microscopy images of live tail NP cells labelled with LysoTracker for 20 min (a) Hoescht, (b) LysoTracker and (c) differential interference contrast DIC and fixed tail NP cells labelled with LipidTOX green neutral lipid stain (d) Hoescht (e) LipidTOX and (f) DIC. (C) Immunofluorescence staining of IVD shows high expression of Slc12a2 in NP. (D) TEM of NP cells showing structures involved in cell–cell communication (a) shows presence of desmosome-like structures (b) as well as gap junctions (c & d, white arrows). (E) Immunofluorescence shows expression of Cd81 and Cd109 in NP. FACS analysis shows that Cd81 and Cd109 have high co-expression with NP marker <t>Cd24.</t> (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
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    Characterization of NP structures by transmission electron microscopy (TEM) and immunohistochemistry. (A) Transmission electron micrographs depict vacuole structures inside NP cells (labelled with white V) and immunofluorescence staining of NP cells show expression for lysosomal marker Lamp1. Lamp 1 is highly expressed in NP with presence of large vacuolar structures (IF and transmission electron microscopy) These structures resent with heterogenous staining for lysosomal marker Lamp 1 indicating potential existence of different types of vacuoles. (B) Microscopy images of live tail NP cells labelled with LysoTracker for 20 min (a) Hoescht, (b) LysoTracker and (c) differential interference contrast DIC and fixed tail NP cells labelled with LipidTOX green neutral lipid stain (d) Hoescht (e) LipidTOX and (f) DIC. (C) Immunofluorescence staining of IVD shows high expression of Slc12a2 in NP. (D) TEM of NP cells showing structures involved in cell–cell communication (a) shows presence of desmosome-like structures (b) as well as gap junctions (c & d, white arrows). (E) Immunofluorescence shows expression of Cd81 and Cd109 in NP. FACS analysis shows that Cd81 and Cd109 have high co-expression with NP marker <t>Cd24.</t> (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
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    Characterization of NP structures by transmission electron microscopy (TEM) and immunohistochemistry. (A) Transmission electron micrographs depict vacuole structures inside NP cells (labelled with white V) and immunofluorescence staining of NP cells show expression for lysosomal marker Lamp1. Lamp 1 is highly expressed in NP with presence of large vacuolar structures (IF and transmission electron microscopy) These structures resent with heterogenous staining for lysosomal marker Lamp 1 indicating potential existence of different types of vacuoles. (B) Microscopy images of live tail NP cells labelled with LysoTracker for 20 min (a) Hoescht, (b) LysoTracker and (c) differential interference contrast DIC and fixed tail NP cells labelled with LipidTOX green neutral lipid stain (d) Hoescht (e) LipidTOX and (f) DIC. (C) Immunofluorescence staining of IVD shows high expression of Slc12a2 in NP. (D) TEM of NP cells showing structures involved in cell–cell communication (a) shows presence of desmosome-like structures (b) as well as gap junctions (c & d, white arrows). (E) Immunofluorescence shows expression of Cd81 and Cd109 in NP. FACS analysis shows that Cd81 and Cd109 have high co-expression with NP marker <t>Cd24.</t> (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
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    Image Search Results


    Characterization of NP structures by transmission electron microscopy (TEM) and immunohistochemistry. (A) Transmission electron micrographs depict vacuole structures inside NP cells (labelled with white V) and immunofluorescence staining of NP cells show expression for lysosomal marker Lamp1. Lamp 1 is highly expressed in NP with presence of large vacuolar structures (IF and transmission electron microscopy) These structures resent with heterogenous staining for lysosomal marker Lamp 1 indicating potential existence of different types of vacuoles. (B) Microscopy images of live tail NP cells labelled with LysoTracker for 20 min (a) Hoescht, (b) LysoTracker and (c) differential interference contrast DIC and fixed tail NP cells labelled with LipidTOX green neutral lipid stain (d) Hoescht (e) LipidTOX and (f) DIC. (C) Immunofluorescence staining of IVD shows high expression of Slc12a2 in NP. (D) TEM of NP cells showing structures involved in cell–cell communication (a) shows presence of desmosome-like structures (b) as well as gap junctions (c & d, white arrows). (E) Immunofluorescence shows expression of Cd81 and Cd109 in NP. FACS analysis shows that Cd81 and Cd109 have high co-expression with NP marker Cd24. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: Matrix Biology Plus

    Article Title: PRIMUS: Comprehensive proteomics of mouse intervertebral discs that inform novel biology and relevance to human disease modelling

    doi: 10.1016/j.mbplus.2021.100082

    Figure Lengend Snippet: Characterization of NP structures by transmission electron microscopy (TEM) and immunohistochemistry. (A) Transmission electron micrographs depict vacuole structures inside NP cells (labelled with white V) and immunofluorescence staining of NP cells show expression for lysosomal marker Lamp1. Lamp 1 is highly expressed in NP with presence of large vacuolar structures (IF and transmission electron microscopy) These structures resent with heterogenous staining for lysosomal marker Lamp 1 indicating potential existence of different types of vacuoles. (B) Microscopy images of live tail NP cells labelled with LysoTracker for 20 min (a) Hoescht, (b) LysoTracker and (c) differential interference contrast DIC and fixed tail NP cells labelled with LipidTOX green neutral lipid stain (d) Hoescht (e) LipidTOX and (f) DIC. (C) Immunofluorescence staining of IVD shows high expression of Slc12a2 in NP. (D) TEM of NP cells showing structures involved in cell–cell communication (a) shows presence of desmosome-like structures (b) as well as gap junctions (c & d, white arrows). (E) Immunofluorescence shows expression of Cd81 and Cd109 in NP. FACS analysis shows that Cd81 and Cd109 have high co-expression with NP marker Cd24. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Isolated cells were fixed in 3.2 % (w/v) formaldehyde, blocked with 2% (w/v) BSA, and incubated with rabbit Cd109 (1:100) (Abcam, ab203588), rabbit Cd81 (1:50) (ProSci, 5195) or rat Cd24 (1:50) antibodies followed by incubation with appropriate secondary antibodies (1:200) (donkey anti-rabbit or donkey anti-rat) conjugated with Alexa 488 or Cyanine 3 (Cy3) respectively.

    Techniques: Transmission Assay, Electron Microscopy, Immunohistochemistry, Immunofluorescence, Staining, Expressing, Marker, Microscopy