Journal: Heliyon

Article Title: Low expression of RACK1 is associated with metastasis and worse prognosis in cholangiocarcinoma

doi: 10.1016/j.heliyon.2024.e27366

Figure Lengend Snippet: The immunohistochemistry result of RACK1 protein level in the clinical cholangiocarcinoma samples from Zhongshan Hospital A. Representative immunostaining images of RACK1 in 2 cholangiocarcinoma cases (scale bars = 100 μm). B. The representative figures of RACK1 expression intensities in tumor and adjacent normal tissues. The expression intensity was graded as negative, weak, moderate, or strong (scale bars = 100 μm). C. The RACK1 expression staining intensities in tumor tissues and peritumoral normal tissues. ( P < 0.001). D. The composite expression score was used to evaluate the IHC staining intensity. The higher expression of RACK1 was observed in tumor tissues than in paired adjacent normal tissues ( P < 0.001). E. The prognostic significance of RACK1 expression in Zhongshan Hospital's surveillance cohort. The OS of patients with high RACK1 expression was significantly longer than that of patients with low RACK1 expression ( P < 0.001). *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Article Snippet: The primary antibody was anti-RACK1 antibody (1:1000; Santa Cruz Biotechnology, Lot # sc-17754).

Techniques: Immunohistochemistry, Immunostaining, Expressing, Staining

Journal: Heliyon

Article Title: Low expression of RACK1 is associated with metastasis and worse prognosis in cholangiocarcinoma

doi: 10.1016/j.heliyon.2024.e27366

Figure Lengend Snippet: The relation between clinical parameters and the expression of RACK1.

Article Snippet: The primary antibody was anti-RACK1 antibody (1:1000; Santa Cruz Biotechnology, Lot # sc-17754).

Techniques: Expressing

Journal: Heliyon

Article Title: Low expression of RACK1 is associated with metastasis and worse prognosis in cholangiocarcinoma

doi: 10.1016/j.heliyon.2024.e27366

Figure Lengend Snippet: Univariate and multivariate analysis for overall survival.

Article Snippet: The primary antibody was anti-RACK1 antibody (1:1000; Santa Cruz Biotechnology, Lot # sc-17754).

Techniques:

Journal: Heliyon

Article Title: Low expression of RACK1 is associated with metastasis and worse prognosis in cholangiocarcinoma

doi: 10.1016/j.heliyon.2024.e27366

Figure Lengend Snippet: The mRNA expression pattern of RACK1 in GEO and TCGA cholangiocarcinoma datasets A. The RACK1 protein score in tumor tissues of patients with lymph node metastasis and without lymph node metastasis. The RACK1 scores were lower in patients with lymph node metastasis than in patients without lymph node metastasis ( P < 0.001). B. The RACK1 protein score in tumor tissues of patients with different tumor number. The RACK1 scores were lower in patients with multiple tumors than with single tumor ( P < 0.05). C. The RACK1 score in tumor tissues of patients with different differentiations was not significant ( P > 0.05). D and E. The expression pattern of RACK1 between tumor tissues and adjacent normal tissues in GEO datasets. Compared with the adjacent normal tissue, the aberrant expression of RACK1 was observed in the tumor tissues in the GSE76311(N = 90, P < 0.001) and GSE107943 (N = 27, P < 0.001) datasets. F. The expression pattern of RACK1 between tumor tissues and paired adjacent normal tissues in TCGA database. Compared with the adjacent normal tissue, the aberrant expression of RACK1 was observed in the tumor tissues (N = 9, P < 0.01). G. The correlation between the lymph node metastasis and the RACK1 expression level. The expression level of RACK1 was significantly lower in patients with lymph node metastasis. (N = 36, P < 0.05). *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Article Snippet: The primary antibody was anti-RACK1 antibody (1:1000; Santa Cruz Biotechnology, Lot # sc-17754).

Techniques: Expressing

Journal: Heliyon

Article Title: Low expression of RACK1 is associated with metastasis and worse prognosis in cholangiocarcinoma

doi: 10.1016/j.heliyon.2024.e27366

Figure Lengend Snippet: The potential underlying mechanism for the biological effect of knockdown of RACK1 on cholangiocarcinoma (A) qRT-PCR results confirmed that the RACK1 mRNA expression was significantly lower in siRNA-1 and siRNA-2 RBE and HCCC-9810 cell lines compared with the control, siRNA-SCR RBE and HCCC-9810 cell lines. (B) The knockdown of RACK1 expression induced the downregulation of E-cadherin, ZO-1, and claudin and the upregulation of vimentin in RBE and HCCC-9810 cell lines. (C) Representative view and statistics of RBE and HCCC-9810 cell lines migrating from the upper Transwell chambers into the lower chambers. The knockdown of RACK1 expression promoted the migration of RBE and HCCC-9810 cell lines. (D) Representative view and statistics of RBE and HCCC-9810 cell lines migration on a wound healing assay. The knockdown of RACK1 expression promoted the migration ability of RBE and HCCC-9810 cell lines.

Article Snippet: The primary antibody was anti-RACK1 antibody (1:1000; Santa Cruz Biotechnology, Lot # sc-17754).

Techniques: Quantitative RT-PCR, Expressing, Migration, Wound Healing Assay

Journal: Heliyon

Article Title: Low expression of RACK1 is associated with metastasis and worse prognosis in cholangiocarcinoma

doi: 10.1016/j.heliyon.2024.e27366

Figure Lengend Snippet: Effect of RACK1 depletion on the proliferation and apoptosis. (A) Representative view and statistics of RBE and HCCC-9810 cells in the colony formation assay. The knockdown of RACK1 expression had no significant effect on the colony number of RBE and HCCC-9810 cells. (B) Cell proliferation potential measured by CCK-8 assay. The knockdown of RACK1 expression had no significant effect on the cell viability of RBE and HCCC-9810 cells. (C) Cell apoptosis measured by flow cytometric analysis. The knockdown of RACK1 expression had no significant effect on the cell apoptosis of RBE and HCCC-9810 cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Article Snippet: The primary antibody was anti-RACK1 antibody (1:1000; Santa Cruz Biotechnology, Lot # sc-17754).

Techniques: Colony Assay, Expressing, CCK-8 Assay

Journal: Heliyon

Article Title: Low expression of RACK1 is associated with metastasis and worse prognosis in cholangiocarcinoma

doi: 10.1016/j.heliyon.2024.e27366

Figure Lengend Snippet: The potential underlying mechanism for the biological effect of overexpression of RACK1 on cholangiocarcinoma (A) The overexpression of RACK1 expression induced the upregulation of E-cadherin, ZO-1, and claudin and the downregulation of vimentin in RBE and HCCC-9810 cell lines. (B) Representative view and statistics of RBE and HCCC-9810 cell lines migrating from upper Transwell chambers into lower ones. The overexpression of RACK1 expression inhibited the migration ability of RBE and HCCC-9810 cell lines. (C) Representative view and statistics of RBE and HCCC-9810 cell lines migration on a wound healing assay. The overexpression of RACK1 expression inhibited the migration ability of RBE and HCCC-9810 cell lines.

Article Snippet: The primary antibody was anti-RACK1 antibody (1:1000; Santa Cruz Biotechnology, Lot # sc-17754).

Techniques: Over Expression, Expressing, Migration, Wound Healing Assay