cultivation conditions c necator h16  (ATCC)


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    ATCC cultivation conditions c necator h16
    Heterotrophic growth of C. <t>necator</t> <t>H16</t> cells during a 48 h bioreactor fermentation. A) Log 10 OD 600nm and soluble hydrogenase activity (by NAD+ reduction) with time; B) dO 2 and pH variation with time. These graphs are based on three biological replicates and represent their mean values with standard deviation given.
    Cultivation Conditions C Necator H16, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "An analysis of the changes in soluble hydrogenase and global gene expression in Cupriavidus necator (Ralstonia eutropha) H16 grown in heterotrophic diauxic batch culture"

    Article Title: An analysis of the changes in soluble hydrogenase and global gene expression in Cupriavidus necator (Ralstonia eutropha) H16 grown in heterotrophic diauxic batch culture

    Journal: Microbial Cell Factories

    doi: 10.1186/s12934-015-0226-4

    Heterotrophic growth of C. necator H16 cells during a 48 h bioreactor fermentation. A) Log 10 OD 600nm and soluble hydrogenase activity (by NAD+ reduction) with time; B) dO 2 and pH variation with time. These graphs are based on three biological replicates and represent their mean values with standard deviation given.
    Figure Legend Snippet: Heterotrophic growth of C. necator H16 cells during a 48 h bioreactor fermentation. A) Log 10 OD 600nm and soluble hydrogenase activity (by NAD+ reduction) with time; B) dO 2 and pH variation with time. These graphs are based on three biological replicates and represent their mean values with standard deviation given.

    Techniques Used: Activity Assay, Standard Deviation

    2) Product Images from "Comparative and Functional Genomics of Rhodococcus opacus PD630 for Biofuels Development"

    Article Title: Comparative and Functional Genomics of Rhodococcus opacus PD630 for Biofuels Development

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1002219

    Screens of four bacterial species for growth on carbohydrates and alcohols. a) Compounds were clustered according to how R. opacus PD630, R. jostii RHA1, C. glutamicum 13032, and R. eutropha H16 were able to grow on oligosaccharides from 2–4 days. Yellow indicates evidence of growth. b) alcohol and monosaccharide compounds were clustered according to bacterial growth as in a. c) Comparison of three Rhodococcus chromosomes revealed that R. opacus B4 and R. opacus PD630 shared two divergent operons dedicated to galactose and oligogalactoside metabolism but R. jostii RHA1 only had a small piece of this chromosomal region containing the GalK and GalT genes omitting α- and β-galactosidases, Solute Binding Protein (SBP), two Solute Binding Protein Transporter (SBPT) proteins, and a DeoR family transcriptional regulatory protein.
    Figure Legend Snippet: Screens of four bacterial species for growth on carbohydrates and alcohols. a) Compounds were clustered according to how R. opacus PD630, R. jostii RHA1, C. glutamicum 13032, and R. eutropha H16 were able to grow on oligosaccharides from 2–4 days. Yellow indicates evidence of growth. b) alcohol and monosaccharide compounds were clustered according to bacterial growth as in a. c) Comparison of three Rhodococcus chromosomes revealed that R. opacus B4 and R. opacus PD630 shared two divergent operons dedicated to galactose and oligogalactoside metabolism but R. jostii RHA1 only had a small piece of this chromosomal region containing the GalK and GalT genes omitting α- and β-galactosidases, Solute Binding Protein (SBP), two Solute Binding Protein Transporter (SBPT) proteins, and a DeoR family transcriptional regulatory protein.

    Techniques Used: Binding Assay

    3) Product Images from "Bioconversion Process of Polyethylene from Waste Tetra Pak® Packaging to Polyhydroxyalkanoates"

    Article Title: Bioconversion Process of Polyethylene from Waste Tetra Pak® Packaging to Polyhydroxyalkanoates

    Journal: Polymers

    doi: 10.3390/polym14142840

    The ESI-MS (positive-ion mode) spectrum of PHA oligomers, obtained via partial thermal degradation of the biopolyester produced by C. necator H16 in TSB using PE-T as an additional carbon source.
    Figure Legend Snippet: The ESI-MS (positive-ion mode) spectrum of PHA oligomers, obtained via partial thermal degradation of the biopolyester produced by C. necator H16 in TSB using PE-T as an additional carbon source.

    Techniques Used: Produced

    1 H-NMR of PHA produced by C. necator utilising PE-T as an additional carbon source in TSB medium.
    Figure Legend Snippet: 1 H-NMR of PHA produced by C. necator utilising PE-T as an additional carbon source in TSB medium.

    Techniques Used: Nuclear Magnetic Resonance, Produced

    Growth observation of C. necator H16 with 0.5 g of PE-T in either TSB or BSM medium after 72 h of incubation at 30 °C. Viable count (Log 10 CFU/mL) data points at times of 0, 3, 6, 9, 15, 21, 24, 27, 30, 40, 44, 48, 54, 65, and 72 h are the mean values of triplicate experiments (n = 3). Error bars represent the standard error of the mean values.
    Figure Legend Snippet: Growth observation of C. necator H16 with 0.5 g of PE-T in either TSB or BSM medium after 72 h of incubation at 30 °C. Viable count (Log 10 CFU/mL) data points at times of 0, 3, 6, 9, 15, 21, 24, 27, 30, 40, 44, 48, 54, 65, and 72 h are the mean values of triplicate experiments (n = 3). Error bars represent the standard error of the mean values.

    Techniques Used: Incubation

    4) Product Images from "CO synthesized from the central one-carbon pool as source for the iron carbonyl in O2-tolerant [NiFe]-hydrogenase"

    Article Title: CO synthesized from the central one-carbon pool as source for the iron carbonyl in O2-tolerant [NiFe]-hydrogenase

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1614656113

    Retarded maturation of [NiFe]-hydrogenase large subunits in the Δ hypX strain can be complemented by external addition of gaseous CO. The R. eutropha strains H16 (wild type) and HF469 (Δ hypX ) were cultivated under lithoautotrophic conditions
    Figure Legend Snippet: Retarded maturation of [NiFe]-hydrogenase large subunits in the Δ hypX strain can be complemented by external addition of gaseous CO. The R. eutropha strains H16 (wild type) and HF469 (Δ hypX ) were cultivated under lithoautotrophic conditions

    Techniques Used:

    Glycine auxotrophy of a Δ glyA mutant of R. eutropha . The R. eutropha strains H16 (wt) and HF965 (Δ glyA ) were plated on solid FN medium without ( A ) or with ( B ) 5 mM glycine and cultivated for 72 h under air at 30 °C.
    Figure Legend Snippet: Glycine auxotrophy of a Δ glyA mutant of R. eutropha . The R. eutropha strains H16 (wt) and HF965 (Δ glyA ) were plated on solid FN medium without ( A ) or with ( B ) 5 mM glycine and cultivated for 72 h under air at 30 °C.

    Techniques Used: Mutagenesis

    Lithoautotrophic growth of the HypX-deficient R. eutropha derivative in the absence and presence of externally added carbon monoxide gas. Strains R. eutropha HF469 (Δ hypX , gray lines) and H16 (wild-type, black lines) were pregrown in fructose-ammonium
    Figure Legend Snippet: Lithoautotrophic growth of the HypX-deficient R. eutropha derivative in the absence and presence of externally added carbon monoxide gas. Strains R. eutropha HF469 (Δ hypX , gray lines) and H16 (wild-type, black lines) were pregrown in fructose-ammonium

    Techniques Used:

    5) Product Images from "Revisiting the Single Cell Protein Application of Cupriavidus necator H16 and Recovering Bioplastic Granules Simultaneously"

    Article Title: Revisiting the Single Cell Protein Application of Cupriavidus necator H16 and Recovering Bioplastic Granules Simultaneously

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0078528

    The appearance of the faeces of the (A) Control animals and (B) Test animals fed with lyophilized cells of C. necator H16 containing 39 wt% PHB.
    Figure Legend Snippet: The appearance of the faeces of the (A) Control animals and (B) Test animals fed with lyophilized cells of C. necator H16 containing 39 wt% PHB.

    Techniques Used:

    6) Product Images from "A Transcription Factor-Based Biosensor for Detection of Itaconic Acid"

    Article Title: A Transcription Factor-Based Biosensor for Detection of Itaconic Acid

    Journal: ACS Synthetic Biology

    doi: 10.1021/acssynbio.8b00057

    Influence of ItcR on inducible gene expression. Absolute normalized fluorescence (in arbitrary units) of (A) E. coli MG1655 and (B) C. necator H16 harboring the Y. pseudotuberculosis ( Yp ) and P. aeruginosa ( Pa ) itaconate-inducible systems composed of promoter and transcriptional regulator (ItcR/P), and promoter-only (P) implementation in the absence and presence of 5 mM itaconate. Single time-point fluorescence measurements were taken 6 h after inducer addition. The promoterless reporter plasmid pEH006E was employed as negative control. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p
    Figure Legend Snippet: Influence of ItcR on inducible gene expression. Absolute normalized fluorescence (in arbitrary units) of (A) E. coli MG1655 and (B) C. necator H16 harboring the Y. pseudotuberculosis ( Yp ) and P. aeruginosa ( Pa ) itaconate-inducible systems composed of promoter and transcriptional regulator (ItcR/P), and promoter-only (P) implementation in the absence and presence of 5 mM itaconate. Single time-point fluorescence measurements were taken 6 h after inducer addition. The promoterless reporter plasmid pEH006E was employed as negative control. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p

    Techniques Used: Expressing, Fluorescence, Plasmid Preparation, Negative Control

    7) Product Images from "Characterization of the norB Gene, Encoding Nitric Oxide Reductase, in the Nondenitrifying Cyanobacterium Synechocystis sp. Strain PCC6803"

    Article Title: Characterization of the norB Gene, Encoding Nitric Oxide Reductase, in the Nondenitrifying Cyanobacterium Synechocystis sp. Strain PCC6803

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.68.2.668-672.2002

    Nitrous oxide production by R . eutropha strains. Symbols: ◊, wild-type H16; ▴, nitric oxide reductase-negative mutant HF420; •, HF420 complemented with the norB gene region from Synechocystis (pGE409); ○, HF420 complemented with Synechocystis norB under the control of the norAB promoter of R . eutropha (pGE453).
    Figure Legend Snippet: Nitrous oxide production by R . eutropha strains. Symbols: ◊, wild-type H16; ▴, nitric oxide reductase-negative mutant HF420; •, HF420 complemented with the norB gene region from Synechocystis (pGE409); ○, HF420 complemented with Synechocystis norB under the control of the norAB promoter of R . eutropha (pGE453).

    Techniques Used: Mutagenesis

    8) Product Images from "RubisCO selection using the vigorously aerobic and metabolically versatile bacterium Ralstonia eutropha"

    Article Title: RubisCO selection using the vigorously aerobic and metabolically versatile bacterium Ralstonia eutropha

    Journal: The FEBS journal

    doi: 10.1111/febs.13774

    Complementation of R. eutropha strain H16ΔLS with native and heterologous RubisCOs. (A) Schematic of a ~4.5-kb region amplified from cbb c operon in R. eutropha strain H16, which includes the coding sequences for cbbR , cbbL , cbbS , and cbbX . This product was used as a template for replacing cbbLS Re genes with heterologous RubisCO-encoding genes, utilizing the engineered MfeI or a ClaI , and SpeI recognition sequences. The form-I sequences from R. palustris ( cbbLS Rp ) and S. elongatus strain PCC 6301 ( rbcLS Se ) both included the native intergenic spacer DNA from the respective organisms. The ~1.4-kb R. palustris cbbM Rp gene and the ~1.3-kb A. fulgidus rbcL2 Af gene both encode form II and form III enzymes, respectively. (B) Vector map of the broad-host range plasmid, p90, which was used for trans expression of RubisCO genes in R. eutropha . It features the native cbb promoter (red; between NcoI and SacI sites) to drive the expression of genes cloned into the multiple-cloning site (green; from SacI to HindIII ). (C) Autotrophic growth phenotypes conferred by the RubisCO genes that had been recombined into the cbb c locus in R. eutropha strain H16ΔLS. For all samples, data points represent average values obtained from triplicate cultures, along with the respective standard deviations. Data is representative of two independent experiments that gave similar results.
    Figure Legend Snippet: Complementation of R. eutropha strain H16ΔLS with native and heterologous RubisCOs. (A) Schematic of a ~4.5-kb region amplified from cbb c operon in R. eutropha strain H16, which includes the coding sequences for cbbR , cbbL , cbbS , and cbbX . This product was used as a template for replacing cbbLS Re genes with heterologous RubisCO-encoding genes, utilizing the engineered MfeI or a ClaI , and SpeI recognition sequences. The form-I sequences from R. palustris ( cbbLS Rp ) and S. elongatus strain PCC 6301 ( rbcLS Se ) both included the native intergenic spacer DNA from the respective organisms. The ~1.4-kb R. palustris cbbM Rp gene and the ~1.3-kb A. fulgidus rbcL2 Af gene both encode form II and form III enzymes, respectively. (B) Vector map of the broad-host range plasmid, p90, which was used for trans expression of RubisCO genes in R. eutropha . It features the native cbb promoter (red; between NcoI and SacI sites) to drive the expression of genes cloned into the multiple-cloning site (green; from SacI to HindIII ). (C) Autotrophic growth phenotypes conferred by the RubisCO genes that had been recombined into the cbb c locus in R. eutropha strain H16ΔLS. For all samples, data points represent average values obtained from triplicate cultures, along with the respective standard deviations. Data is representative of two independent experiments that gave similar results.

    Techniques Used: Amplification, Periodic Counter-current Chromatography, Plasmid Preparation, Expressing, Clone Assay

    9) Product Images from "PCR Detection of Genes Encoding Nitrite Reductase in Denitrifying Bacteria"

    Article Title: PCR Detection of Genes Encoding Nitrite Reductase in Denitrifying Bacteria

    Journal: Applied and Environmental Microbiology

    doi:

    Agarose gels of PCR fragments and Southern blot hybridizations. Arrows indicate fragments of the expected sizes. Panels: a, cd 1 -nir primers; b, cd 1 probe; c, Cu- nir primers; d, Cu probe. The upper row has molecular size markers in lane 1, P. stutzeri ATCC 14405 in lane 2, P. stutzeri CCUG 29240 in lane 3, P. fluorescens ATCC 33512 in lane 4, P. fluorescens Mi32 in lane 5, R. eutropha CCUG 13724 in lane 6, A. eutrophus ATCC 17699 in lane 7, P. aeruginosa CCUG 241 in lane 8, P. aeruginosa Mi11 in lane 9, P. denitrificans Pd1222 in lane 10, P. denitrificans ATCC 19367 in lane 11, P. denitrificans ATCC 13867 in lane 12, P. denitrificans CCUG 2519 in lane 13, P. putida CCUG 2479 in lane 14, and molecular size markers in lane 15. The lower row has molecular size markers in lane 16, A. faecalis ATCC 8750 in lane 17, A. faecalis ATCC 19018 in lane 18, P. aureofaciens ATCC 13985 in lane 19, A. denitrificans ATCC 15173 in lane 20, A. cycloclastes ATCC 21921 in lane 21, sludge L1 in lane 22, sludge L2 in lane 23, sludge P1 in lane 24, E. coli TG1 in lane 25, S. aureus 8325-4 in lane 26, a negative control in lane 27, and molecular size markers in lane 28. The molecular size markers consisted of a 100-bp ladder with the 800-bp fragment twice as intense as the other bands (Pharmacia).
    Figure Legend Snippet: Agarose gels of PCR fragments and Southern blot hybridizations. Arrows indicate fragments of the expected sizes. Panels: a, cd 1 -nir primers; b, cd 1 probe; c, Cu- nir primers; d, Cu probe. The upper row has molecular size markers in lane 1, P. stutzeri ATCC 14405 in lane 2, P. stutzeri CCUG 29240 in lane 3, P. fluorescens ATCC 33512 in lane 4, P. fluorescens Mi32 in lane 5, R. eutropha CCUG 13724 in lane 6, A. eutrophus ATCC 17699 in lane 7, P. aeruginosa CCUG 241 in lane 8, P. aeruginosa Mi11 in lane 9, P. denitrificans Pd1222 in lane 10, P. denitrificans ATCC 19367 in lane 11, P. denitrificans ATCC 13867 in lane 12, P. denitrificans CCUG 2519 in lane 13, P. putida CCUG 2479 in lane 14, and molecular size markers in lane 15. The lower row has molecular size markers in lane 16, A. faecalis ATCC 8750 in lane 17, A. faecalis ATCC 19018 in lane 18, P. aureofaciens ATCC 13985 in lane 19, A. denitrificans ATCC 15173 in lane 20, A. cycloclastes ATCC 21921 in lane 21, sludge L1 in lane 22, sludge L2 in lane 23, sludge P1 in lane 24, E. coli TG1 in lane 25, S. aureus 8325-4 in lane 26, a negative control in lane 27, and molecular size markers in lane 28. The molecular size markers consisted of a 100-bp ladder with the 800-bp fragment twice as intense as the other bands (Pharmacia).

    Techniques Used: Polymerase Chain Reaction, Southern Blot, Negative Control

    10) Product Images from "Global changes in the proteome of Cupriavidus necator H16 during poly-(3-hydroxybutyrate) synthesis from various biodiesel by-product substrates"

    Article Title: Global changes in the proteome of Cupriavidus necator H16 during poly-(3-hydroxybutyrate) synthesis from various biodiesel by-product substrates

    Journal: AMB Express

    doi: 10.1186/s13568-016-0206-z

    Consumption of glycerol, free fatty acids, and nitrogen by C. necator H16. a Consumption of glycerol by C. necator H16 in cultures containing REG-GB (glycerol bottom) and REG-80 (glycerol); b Consumption of fatty acids by C. necator H16 in cultures containing REG-GB (glycerol bottom) and REG-FFA (free fatty acids); c Consumption of ammonium nitrogen (NH 4 -N) by C. necator H16 in cultures containing REG-GB (glycerol bottom), REG-80 (glycerol), and REG-FFA (free fatty acids)
    Figure Legend Snippet: Consumption of glycerol, free fatty acids, and nitrogen by C. necator H16. a Consumption of glycerol by C. necator H16 in cultures containing REG-GB (glycerol bottom) and REG-80 (glycerol); b Consumption of fatty acids by C. necator H16 in cultures containing REG-GB (glycerol bottom) and REG-FFA (free fatty acids); c Consumption of ammonium nitrogen (NH 4 -N) by C. necator H16 in cultures containing REG-GB (glycerol bottom), REG-80 (glycerol), and REG-FFA (free fatty acids)

    Techniques Used:

    Differential expression level of proteins of glycerol metabolism pathway, the TCA cycle, and glyoxylate shunt pathway in C. necator H16 grown in REG-GB, REG-FFA and REG-80 at 24 h pi. Expression levels are presented as normalized relative Z-score expression ratios (Znet > 1.65). The green colored numbers indicate up-regulation of protein expression; red colored numbers indicate down-regulation of protein expression. White color no significant change. GB-24, REG-GB (glycerol bottom) at 24 h pi; R80-24, REG-80 (glycerol) at 24 h pi; FFA-24, REG-FFA (free fatty acids) at 24 h pi
    Figure Legend Snippet: Differential expression level of proteins of glycerol metabolism pathway, the TCA cycle, and glyoxylate shunt pathway in C. necator H16 grown in REG-GB, REG-FFA and REG-80 at 24 h pi. Expression levels are presented as normalized relative Z-score expression ratios (Znet > 1.65). The green colored numbers indicate up-regulation of protein expression; red colored numbers indicate down-regulation of protein expression. White color no significant change. GB-24, REG-GB (glycerol bottom) at 24 h pi; R80-24, REG-80 (glycerol) at 24 h pi; FFA-24, REG-FFA (free fatty acids) at 24 h pi

    Techniques Used: Expressing

    Venn diagram showing the number and relationship of proteins differentially expressed for the Substrate comparisons. Circles represent the total of proteins identified in proteome of C. necator H16 grown in REG glycerol bottom (REG-GB), REG free fatty acid (REG-FFA) and REG glycerol (REG-80) for 24 h. The number of proteins differentially expressed is indicated in each subset. Green color indicates up-regulation and red color indicates down-regulation. Only the 10 % of proteins showing the largest variation (Znet > ± 1.65) are listed
    Figure Legend Snippet: Venn diagram showing the number and relationship of proteins differentially expressed for the Substrate comparisons. Circles represent the total of proteins identified in proteome of C. necator H16 grown in REG glycerol bottom (REG-GB), REG free fatty acid (REG-FFA) and REG glycerol (REG-80) for 24 h. The number of proteins differentially expressed is indicated in each subset. Green color indicates up-regulation and red color indicates down-regulation. Only the 10 % of proteins showing the largest variation (Znet > ± 1.65) are listed

    Techniques Used:

    Cell dry weight (dcw) and PHB synthesis by C. necator H16 in RMM with a REG-80, b REG-GB, and c REG-FFA as substrates
    Figure Legend Snippet: Cell dry weight (dcw) and PHB synthesis by C. necator H16 in RMM with a REG-80, b REG-GB, and c REG-FFA as substrates

    Techniques Used:

    Differential protein expression levels in the fatty acid oxidation pathway in C. necator H16 grown with different substrates at 24 h pi. Expression levels are presented as normalized relative Z-score expression ratios (Znet > 1.65). The green colored numbers indicate up-regulation of protein expression; red colored numbers indicate down-regulation and white color no significant change of protein expression. GB-24, REG-GB (glycerol bottom) at 24 h pi; R80-24, REG-80 (glycerol) at 24 h pi; FFA, REG-FFA (free fatty acids) at 24 h pi
    Figure Legend Snippet: Differential protein expression levels in the fatty acid oxidation pathway in C. necator H16 grown with different substrates at 24 h pi. Expression levels are presented as normalized relative Z-score expression ratios (Znet > 1.65). The green colored numbers indicate up-regulation of protein expression; red colored numbers indicate down-regulation and white color no significant change of protein expression. GB-24, REG-GB (glycerol bottom) at 24 h pi; R80-24, REG-80 (glycerol) at 24 h pi; FFA, REG-FFA (free fatty acids) at 24 h pi

    Techniques Used: Expressing

    11) Product Images from "Sugar Beet Molasses as a Potential C-Substrate for PHA Production by Cupriavidus necator"

    Article Title: Sugar Beet Molasses as a Potential C-Substrate for PHA Production by Cupriavidus necator

    Journal: Bioengineering

    doi: 10.3390/bioengineering9040154

    Production parameters of bacterium Cupriavidus necator B-10646 when grown on hydrolyzed sugar beet molasses in 2 L glass flasks under different scenarios of its use: ( a )—20-fold dilution of molasses hydrolysate; ( b )—30-fold dilution of molasses hydrolysate with the addition of phosphorus and glucose to the culture (indicated by arrows).
    Figure Legend Snippet: Production parameters of bacterium Cupriavidus necator B-10646 when grown on hydrolyzed sugar beet molasses in 2 L glass flasks under different scenarios of its use: ( a )—20-fold dilution of molasses hydrolysate; ( b )—30-fold dilution of molasses hydrolysate with the addition of phosphorus and glucose to the culture (indicated by arrows).

    Techniques Used:

    Production parameters of Cupriavidus necator B-10646 culture when cultivated in the 8 L fermenter using 20-fold dilution of molasses hydrolysate, concentrated inoculum (5 g/L), and a larger amount of glucose and phosphorus supplementation as a source of carbon, nitrogen, and mineral elements at the first stage with feeding with phosphorus and glucose; at the second stage—in a nitrogen-free medium with culture fed with glucose and phosphorus (feeds are shown by arrows): the concentration of the total cell biomass in the culture (X, g/L) and the polymer in the cells (PHA% of CDW); specific cell growth rate (µX h −1 ) and specific polymer synthesis rate (µPHA, h −1 ).
    Figure Legend Snippet: Production parameters of Cupriavidus necator B-10646 culture when cultivated in the 8 L fermenter using 20-fold dilution of molasses hydrolysate, concentrated inoculum (5 g/L), and a larger amount of glucose and phosphorus supplementation as a source of carbon, nitrogen, and mineral elements at the first stage with feeding with phosphorus and glucose; at the second stage—in a nitrogen-free medium with culture fed with glucose and phosphorus (feeds are shown by arrows): the concentration of the total cell biomass in the culture (X, g/L) and the polymer in the cells (PHA% of CDW); specific cell growth rate (µX h −1 ) and specific polymer synthesis rate (µPHA, h −1 ).

    Techniques Used: Concentration Assay

    Production parameters of the bacterial culture Cupriavidus necator B-10646 when grown on hydrolyzed sugar beet molasses in the 8 L fermenter under different versions of feeding the culture with nutritive elements: ( a )—20-fold dilution of molasses hydrolysate; culture feeding with phosphorus at the first stage, and feeding with phosphorus + glucose at the second stage; ( b )—20-fold dilution of molasses hydrolysate with feeding the culture with phosphorus and glucose at the first and second stages (indicated by arrows).
    Figure Legend Snippet: Production parameters of the bacterial culture Cupriavidus necator B-10646 when grown on hydrolyzed sugar beet molasses in the 8 L fermenter under different versions of feeding the culture with nutritive elements: ( a )—20-fold dilution of molasses hydrolysate; culture feeding with phosphorus at the first stage, and feeding with phosphorus + glucose at the second stage; ( b )—20-fold dilution of molasses hydrolysate with feeding the culture with phosphorus and glucose at the first and second stages (indicated by arrows).

    Techniques Used:

    Indicators of the culture of Cupriavidus necator B-10646 when grown on molasses hydrolysate and Schlegel’s medium: 1-, 2-, 3-, 10-, 20-, 30-fold dilution of molasses hydrolysate, respectively; 4-complete Schlegel medium; 5-with 50% nitrogen content. Biomass concentration (X, g/L), polymer (% of CDW).
    Figure Legend Snippet: Indicators of the culture of Cupriavidus necator B-10646 when grown on molasses hydrolysate and Schlegel’s medium: 1-, 2-, 3-, 10-, 20-, 30-fold dilution of molasses hydrolysate, respectively; 4-complete Schlegel medium; 5-with 50% nitrogen content. Biomass concentration (X, g/L), polymer (% of CDW).

    Techniques Used: Concentration Assay

    12) Product Images from "Development of an immunoFET biosensor for the detection of biotinylated PCR product"

    Article Title: Development of an immunoFET biosensor for the detection of biotinylated PCR product

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2016.e00188

    Specificity testing of the ISFET-based biosensor to detect the phaE amplicon. (A) Agarose gel electrophoresis of the PCR product and (B) the ΔV G of the biosensor. (M) DNA ladder, (NTC) No template control, (HW) H. walsbyi DSM 16854 (Positive control), (CN) C. necator DSM 428, (BM) B. megaterium DSM 319, (EC) E. coli ATCC 25922, (PA) P. aeruginosa ATCC 9027 and (SH) S. hygroscopicus BTCC 7028.
    Figure Legend Snippet: Specificity testing of the ISFET-based biosensor to detect the phaE amplicon. (A) Agarose gel electrophoresis of the PCR product and (B) the ΔV G of the biosensor. (M) DNA ladder, (NTC) No template control, (HW) H. walsbyi DSM 16854 (Positive control), (CN) C. necator DSM 428, (BM) B. megaterium DSM 319, (EC) E. coli ATCC 25922, (PA) P. aeruginosa ATCC 9027 and (SH) S. hygroscopicus BTCC 7028.

    Techniques Used: Amplification, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Positive Control

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    ATCC cultivation conditions c necator h16
    Heterotrophic growth of C. <t>necator</t> <t>H16</t> cells during a 48 h bioreactor fermentation. A) Log 10 OD 600nm and soluble hydrogenase activity (by NAD+ reduction) with time; B) dO 2 and pH variation with time. These graphs are based on three biological replicates and represent their mean values with standard deviation given.
    Cultivation Conditions C Necator H16, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Heterotrophic growth of C. necator H16 cells during a 48 h bioreactor fermentation. A) Log 10 OD 600nm and soluble hydrogenase activity (by NAD+ reduction) with time; B) dO 2 and pH variation with time. These graphs are based on three biological replicates and represent their mean values with standard deviation given.

    Journal: Microbial Cell Factories

    Article Title: An analysis of the changes in soluble hydrogenase and global gene expression in Cupriavidus necator (Ralstonia eutropha) H16 grown in heterotrophic diauxic batch culture

    doi: 10.1186/s12934-015-0226-4

    Figure Lengend Snippet: Heterotrophic growth of C. necator H16 cells during a 48 h bioreactor fermentation. A) Log 10 OD 600nm and soluble hydrogenase activity (by NAD+ reduction) with time; B) dO 2 and pH variation with time. These graphs are based on three biological replicates and represent their mean values with standard deviation given.

    Article Snippet: Bacterial strain and cultivation conditions C. necator H16 (DSM428, ATCC 17699) was grown heterotrophically in minimal medium (FGN) as described previously [ ] with the omission of the SL-6 trace elements solution [ ].

    Techniques: Activity Assay, Standard Deviation

    Screens of four bacterial species for growth on carbohydrates and alcohols. a) Compounds were clustered according to how R. opacus PD630, R. jostii RHA1, C. glutamicum 13032, and R. eutropha H16 were able to grow on oligosaccharides from 2–4 days. Yellow indicates evidence of growth. b) alcohol and monosaccharide compounds were clustered according to bacterial growth as in a. c) Comparison of three Rhodococcus chromosomes revealed that R. opacus B4 and R. opacus PD630 shared two divergent operons dedicated to galactose and oligogalactoside metabolism but R. jostii RHA1 only had a small piece of this chromosomal region containing the GalK and GalT genes omitting α- and β-galactosidases, Solute Binding Protein (SBP), two Solute Binding Protein Transporter (SBPT) proteins, and a DeoR family transcriptional regulatory protein.

    Journal: PLoS Genetics

    Article Title: Comparative and Functional Genomics of Rhodococcus opacus PD630 for Biofuels Development

    doi: 10.1371/journal.pgen.1002219

    Figure Lengend Snippet: Screens of four bacterial species for growth on carbohydrates and alcohols. a) Compounds were clustered according to how R. opacus PD630, R. jostii RHA1, C. glutamicum 13032, and R. eutropha H16 were able to grow on oligosaccharides from 2–4 days. Yellow indicates evidence of growth. b) alcohol and monosaccharide compounds were clustered according to bacterial growth as in a. c) Comparison of three Rhodococcus chromosomes revealed that R. opacus B4 and R. opacus PD630 shared two divergent operons dedicated to galactose and oligogalactoside metabolism but R. jostii RHA1 only had a small piece of this chromosomal region containing the GalK and GalT genes omitting α- and β-galactosidases, Solute Binding Protein (SBP), two Solute Binding Protein Transporter (SBPT) proteins, and a DeoR family transcriptional regulatory protein.

    Article Snippet: R. eutropha H16 ATCC17699 and C. glutamicum ATCC13032 were from ATCC.

    Techniques: Binding Assay

    The ESI-MS (positive-ion mode) spectrum of PHA oligomers, obtained via partial thermal degradation of the biopolyester produced by C. necator H16 in TSB using PE-T as an additional carbon source.

    Journal: Polymers

    Article Title: Bioconversion Process of Polyethylene from Waste Tetra Pak® Packaging to Polyhydroxyalkanoates

    doi: 10.3390/polym14142840

    Figure Lengend Snippet: The ESI-MS (positive-ion mode) spectrum of PHA oligomers, obtained via partial thermal degradation of the biopolyester produced by C. necator H16 in TSB using PE-T as an additional carbon source.

    Article Snippet: The bacterial strain Cupriavidus necator H16 (NCIMB 10442, ATCC 17699) was used for this investigation.

    Techniques: Produced

    1 H-NMR of PHA produced by C. necator utilising PE-T as an additional carbon source in TSB medium.

    Journal: Polymers

    Article Title: Bioconversion Process of Polyethylene from Waste Tetra Pak® Packaging to Polyhydroxyalkanoates

    doi: 10.3390/polym14142840

    Figure Lengend Snippet: 1 H-NMR of PHA produced by C. necator utilising PE-T as an additional carbon source in TSB medium.

    Article Snippet: The bacterial strain Cupriavidus necator H16 (NCIMB 10442, ATCC 17699) was used for this investigation.

    Techniques: Nuclear Magnetic Resonance, Produced

    Growth observation of C. necator H16 with 0.5 g of PE-T in either TSB or BSM medium after 72 h of incubation at 30 °C. Viable count (Log 10 CFU/mL) data points at times of 0, 3, 6, 9, 15, 21, 24, 27, 30, 40, 44, 48, 54, 65, and 72 h are the mean values of triplicate experiments (n = 3). Error bars represent the standard error of the mean values.

    Journal: Polymers

    Article Title: Bioconversion Process of Polyethylene from Waste Tetra Pak® Packaging to Polyhydroxyalkanoates

    doi: 10.3390/polym14142840

    Figure Lengend Snippet: Growth observation of C. necator H16 with 0.5 g of PE-T in either TSB or BSM medium after 72 h of incubation at 30 °C. Viable count (Log 10 CFU/mL) data points at times of 0, 3, 6, 9, 15, 21, 24, 27, 30, 40, 44, 48, 54, 65, and 72 h are the mean values of triplicate experiments (n = 3). Error bars represent the standard error of the mean values.

    Article Snippet: The bacterial strain Cupriavidus necator H16 (NCIMB 10442, ATCC 17699) was used for this investigation.

    Techniques: Incubation

    Retarded maturation of [NiFe]-hydrogenase large subunits in the Δ hypX strain can be complemented by external addition of gaseous CO. The R. eutropha strains H16 (wild type) and HF469 (Δ hypX ) were cultivated under lithoautotrophic conditions

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: CO synthesized from the central one-carbon pool as source for the iron carbonyl in O2-tolerant [NiFe]-hydrogenase

    doi: 10.1073/pnas.1614656113

    Figure Lengend Snippet: Retarded maturation of [NiFe]-hydrogenase large subunits in the Δ hypX strain can be complemented by external addition of gaseous CO. The R. eutropha strains H16 (wild type) and HF469 (Δ hypX ) were cultivated under lithoautotrophic conditions

    Article Snippet: All R. eutropha strains used in this study were derived from the wild-type R. eutropha H16 (DSM 428, ATCC 17699) or a HypX− derivative of H16, HF469 ( ).

    Techniques:

    Glycine auxotrophy of a Δ glyA mutant of R. eutropha . The R. eutropha strains H16 (wt) and HF965 (Δ glyA ) were plated on solid FN medium without ( A ) or with ( B ) 5 mM glycine and cultivated for 72 h under air at 30 °C.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: CO synthesized from the central one-carbon pool as source for the iron carbonyl in O2-tolerant [NiFe]-hydrogenase

    doi: 10.1073/pnas.1614656113

    Figure Lengend Snippet: Glycine auxotrophy of a Δ glyA mutant of R. eutropha . The R. eutropha strains H16 (wt) and HF965 (Δ glyA ) were plated on solid FN medium without ( A ) or with ( B ) 5 mM glycine and cultivated for 72 h under air at 30 °C.

    Article Snippet: All R. eutropha strains used in this study were derived from the wild-type R. eutropha H16 (DSM 428, ATCC 17699) or a HypX− derivative of H16, HF469 ( ).

    Techniques: Mutagenesis

    Lithoautotrophic growth of the HypX-deficient R. eutropha derivative in the absence and presence of externally added carbon monoxide gas. Strains R. eutropha HF469 (Δ hypX , gray lines) and H16 (wild-type, black lines) were pregrown in fructose-ammonium

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: CO synthesized from the central one-carbon pool as source for the iron carbonyl in O2-tolerant [NiFe]-hydrogenase

    doi: 10.1073/pnas.1614656113

    Figure Lengend Snippet: Lithoautotrophic growth of the HypX-deficient R. eutropha derivative in the absence and presence of externally added carbon monoxide gas. Strains R. eutropha HF469 (Δ hypX , gray lines) and H16 (wild-type, black lines) were pregrown in fructose-ammonium

    Article Snippet: All R. eutropha strains used in this study were derived from the wild-type R. eutropha H16 (DSM 428, ATCC 17699) or a HypX− derivative of H16, HF469 ( ).

    Techniques: