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Illumina Inc 16s rrna gene amplicon libraries
Percentage of total <t>16S</t> <t>rRNA</t> gene <t>amplicon</t> reads assigned to Rickettsia , Spiroplasma and Wolbachia endosymbionts in individuals of social S. dumicola and S. mimosarum . Localities are indicated with MAH (Madagascar, Mahavanana), SAK (Madagascar, Isalo National Park), TANA (Madagascar, Antananarivo), PON (South Africa, Pongola Game Reserve), WEE (South Africa, Weenen Game Farm), KRU (South Africa, Kruger National Park), ADDO (South Africa, Addo), PAA (South Africa, Paarl). First number indicates colony number while second number is the number of the individual spider, belonging to the respective colony.
16s Rrna Gene Amplicon Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/16s rrna gene amplicon libraries/product/Illumina Inc
Average 90 stars, based on 25 article reviews
Price from $9.99 to $1999.99
16s rrna gene amplicon libraries - by Bioz Stars, 2020-07
90/100 stars

Images

1) Product Images from "Male spiders control offspring sex ratio through greater production of female-determining sperm"

Article Title: Male spiders control offspring sex ratio through greater production of female-determining sperm

Journal: Proceedings of the Royal Society B: Biological Sciences

doi: 10.1098/rspb.2017.2887

Percentage of total 16S rRNA gene amplicon reads assigned to Rickettsia , Spiroplasma and Wolbachia endosymbionts in individuals of social S. dumicola and S. mimosarum . Localities are indicated with MAH (Madagascar, Mahavanana), SAK (Madagascar, Isalo National Park), TANA (Madagascar, Antananarivo), PON (South Africa, Pongola Game Reserve), WEE (South Africa, Weenen Game Farm), KRU (South Africa, Kruger National Park), ADDO (South Africa, Addo), PAA (South Africa, Paarl). First number indicates colony number while second number is the number of the individual spider, belonging to the respective colony.
Figure Legend Snippet: Percentage of total 16S rRNA gene amplicon reads assigned to Rickettsia , Spiroplasma and Wolbachia endosymbionts in individuals of social S. dumicola and S. mimosarum . Localities are indicated with MAH (Madagascar, Mahavanana), SAK (Madagascar, Isalo National Park), TANA (Madagascar, Antananarivo), PON (South Africa, Pongola Game Reserve), WEE (South Africa, Weenen Game Farm), KRU (South Africa, Kruger National Park), ADDO (South Africa, Addo), PAA (South Africa, Paarl). First number indicates colony number while second number is the number of the individual spider, belonging to the respective colony.

Techniques Used: Amplification

2) Product Images from "A Walnut-Enriched Diet Affects Gut Microbiome in Healthy Caucasian Subjects: A Randomized, Controlled Trial"

Article Title: A Walnut-Enriched Diet Affects Gut Microbiome in Healthy Caucasian Subjects: A Randomized, Controlled Trial

Journal: Nutrients

doi: 10.3390/nu10020244

( a ) Relative abundance of the 4 dominating bacterial phyla between the walnut and the control diet. Walnut consumption shifted the predominant phyla from Firmicutes (61.2% after walnut consumption vs. 63.9% after control) to Bacteroidetes (30.8% vs. 27.4%). Relative abundance was calculated from the relative abundance of 16S rRNA gene sequences for each bacterial community by using the IMNGS platform. ( b ) Most abundant Operational Taxonomic Units for both walnut and control phase at genus level. Significant different OTUs are marked with by using * and p -values. p -values were calculated using a pairwise Fisher test.
Figure Legend Snippet: ( a ) Relative abundance of the 4 dominating bacterial phyla between the walnut and the control diet. Walnut consumption shifted the predominant phyla from Firmicutes (61.2% after walnut consumption vs. 63.9% after control) to Bacteroidetes (30.8% vs. 27.4%). Relative abundance was calculated from the relative abundance of 16S rRNA gene sequences for each bacterial community by using the IMNGS platform. ( b ) Most abundant Operational Taxonomic Units for both walnut and control phase at genus level. Significant different OTUs are marked with by using * and p -values. p -values were calculated using a pairwise Fisher test.

Techniques Used:

3) Product Images from "CFTR dysregulation drives active selection of the gut microbiome"

Article Title: CFTR dysregulation drives active selection of the gut microbiome

Journal: PLoS Pathogens

doi: 10.1371/journal.ppat.1008251

Mutations in CFTR gene influence the composition of the gut microbiota. The composition of the fecal and cecal microbiota were assessed in CF and non-CF GF recipient mice 1–3 months following FMT with SPF C57BL/6J feces by sequencing of the V3-V4 hypervariable regions of the 16S rRNA gene. See also S1 Fig . (A) Bar graph represents the relative abundances of major genera identified within each sample. Hierarchical clustering was performed based on Bray-Curtis distance, according to ASV distribution at the genus level. The experiment was repeated four times over the course of a year and the numbers (and colors) at the bottom of each column (Red, Brown, Blue, Orange) correspond to an experimental cohort. Fecal and cecal contents were pooled at equal volumes for pair-housed, age, sex, and genotype matched mice within a study prior to DNA extraction (N = 2 mice per genotype per experiment). Black X and Y samples indicate samples from two experiments that used the equivalent of the CF cecal sample of experiment 4 as donor material for the FMT. (B) Multidimensional scaling by ASV’s abundance demonstrate significant clustering by genotype (PERMANOVA, p
Figure Legend Snippet: Mutations in CFTR gene influence the composition of the gut microbiota. The composition of the fecal and cecal microbiota were assessed in CF and non-CF GF recipient mice 1–3 months following FMT with SPF C57BL/6J feces by sequencing of the V3-V4 hypervariable regions of the 16S rRNA gene. See also S1 Fig . (A) Bar graph represents the relative abundances of major genera identified within each sample. Hierarchical clustering was performed based on Bray-Curtis distance, according to ASV distribution at the genus level. The experiment was repeated four times over the course of a year and the numbers (and colors) at the bottom of each column (Red, Brown, Blue, Orange) correspond to an experimental cohort. Fecal and cecal contents were pooled at equal volumes for pair-housed, age, sex, and genotype matched mice within a study prior to DNA extraction (N = 2 mice per genotype per experiment). Black X and Y samples indicate samples from two experiments that used the equivalent of the CF cecal sample of experiment 4 as donor material for the FMT. (B) Multidimensional scaling by ASV’s abundance demonstrate significant clustering by genotype (PERMANOVA, p

Techniques Used: Mouse Assay, Sequencing, DNA Extraction

4) Product Images from "A Walnut-Enriched Diet Affects Gut Microbiome in Healthy Caucasian Subjects: A Randomized, Controlled Trial"

Article Title: A Walnut-Enriched Diet Affects Gut Microbiome in Healthy Caucasian Subjects: A Randomized, Controlled Trial

Journal: Nutrients

doi: 10.3390/nu10020244

( a ) Relative abundance of the 4 dominating bacterial phyla between the walnut and the control diet. Walnut consumption shifted the predominant phyla from Firmicutes (61.2% after walnut consumption vs. 63.9% after control) to Bacteroidetes (30.8% vs. 27.4%). Relative abundance was calculated from the relative abundance of 16S rRNA gene sequences for each bacterial community by using the IMNGS platform. ( b ) Most abundant Operational Taxonomic Units for both walnut and control phase at genus level. Significant different OTUs are marked with by using * and p -values. p -values were calculated using a pairwise Fisher test.
Figure Legend Snippet: ( a ) Relative abundance of the 4 dominating bacterial phyla between the walnut and the control diet. Walnut consumption shifted the predominant phyla from Firmicutes (61.2% after walnut consumption vs. 63.9% after control) to Bacteroidetes (30.8% vs. 27.4%). Relative abundance was calculated from the relative abundance of 16S rRNA gene sequences for each bacterial community by using the IMNGS platform. ( b ) Most abundant Operational Taxonomic Units for both walnut and control phase at genus level. Significant different OTUs are marked with by using * and p -values. p -values were calculated using a pairwise Fisher test.

Techniques Used:

5) Product Images from "Bacteria Associated With a Commercial Mycorrhizal Inoculum: Community Composition and Multifunctional Activity as Assessed by Illumina Sequencing and Culture-Dependent Tools"

Article Title: Bacteria Associated With a Commercial Mycorrhizal Inoculum: Community Composition and Multifunctional Activity as Assessed by Illumina Sequencing and Culture-Dependent Tools

Journal: Frontiers in Plant Science

doi: 10.3389/fpls.2018.01956

Affiliation of the sequences of the 14 bacterial strains showing the best PGP traits with the existing 16S rRNA gene sequences. Phylogenetic analysis was inferred by using the Neighbor-Joining method. The evolutionary distances were computed using the Tamura 3-parameter method. The rate variation among sites was modeled with a gamma distribution (shape parameter = 1). Bootstrap (1,000 replicates) values below 70 are not shown. Evolutionary analyses were conducted in MEGA10.
Figure Legend Snippet: Affiliation of the sequences of the 14 bacterial strains showing the best PGP traits with the existing 16S rRNA gene sequences. Phylogenetic analysis was inferred by using the Neighbor-Joining method. The evolutionary distances were computed using the Tamura 3-parameter method. The rate variation among sites was modeled with a gamma distribution (shape parameter = 1). Bootstrap (1,000 replicates) values below 70 are not shown. Evolutionary analyses were conducted in MEGA10.

Techniques Used:

6) Product Images from "Rett Syndrome: A Focus on Gut Microbiota"

Article Title: Rett Syndrome: A Focus on Gut Microbiota

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms18020344

Bar charts representing the average relative abundance of Rett (RTT, n = 8) and control (CTR, n = 10) microbiota, classified using the 16S rRNA gene. ( A ) Mean relative abundances of fecal bacterial phyla; ( B ) Mean relative abundances of fecal bacterial families.
Figure Legend Snippet: Bar charts representing the average relative abundance of Rett (RTT, n = 8) and control (CTR, n = 10) microbiota, classified using the 16S rRNA gene. ( A ) Mean relative abundances of fecal bacterial phyla; ( B ) Mean relative abundances of fecal bacterial families.

Techniques Used:

7) Product Images from "The Microbiome of Ehrlichia-Infected and Uninfected Lone Star Ticks (Amblyomma americanum)"

Article Title: The Microbiome of Ehrlichia-Infected and Uninfected Lone Star Ticks (Amblyomma americanum)

Journal: PLoS ONE

doi: 10.1371/journal.pone.0146651

Female ticks had significantly higher relative abundance of Ehrlichia compared to males. The relative abundance of Ehrlichia (OTU052) in the 16S rRNA gene libraries was significantly higher in females compared to males (ANOVA F = 3.587, P = 0.064).
Figure Legend Snippet: Female ticks had significantly higher relative abundance of Ehrlichia compared to males. The relative abundance of Ehrlichia (OTU052) in the 16S rRNA gene libraries was significantly higher in females compared to males (ANOVA F = 3.587, P = 0.064).

Techniques Used:

8) Product Images from "Effect of freshwater mussels on the vertical distribution of anaerobic ammonia oxidizers and other nitrogen-transforming microorganisms in upper Mississippi river sediment"

Article Title: Effect of freshwater mussels on the vertical distribution of anaerobic ammonia oxidizers and other nitrogen-transforming microorganisms in upper Mississippi river sediment

Journal: PeerJ

doi: 10.7717/peerj.3536

(A) The mean anammox 16S rRNA gene copies (per gram of sediment) in the presence of mussels were normally distributed (Shapiro–Wilk normality test, W -statistic =0.954, p = 0.773) with depth (Batch 1 data). Error bars represent 1 standard deviation from the mean. (B) Mussels (salmon-colored data) significantly increased the anammox 16S rRNA gene copies at 3 cm depth ( p
Figure Legend Snippet: (A) The mean anammox 16S rRNA gene copies (per gram of sediment) in the presence of mussels were normally distributed (Shapiro–Wilk normality test, W -statistic =0.954, p = 0.773) with depth (Batch 1 data). Error bars represent 1 standard deviation from the mean. (B) Mussels (salmon-colored data) significantly increased the anammox 16S rRNA gene copies at 3 cm depth ( p

Techniques Used: Standard Deviation

Related Articles

Sequencing:

Article Title: Monitoring of microbial dynamics in a drinking water distribution system using the culture-free, user-friendly, MYcrobiota platform
Article Snippet: .. FASTQ-formatted sequences were extracted after paired-end sequencing of the 16S rRNA gene amplicon library using the MiniSeq system (Illumina) and processed using our previously developed bioinformatics analysis service . .. This bioinformatics pipeline consists of 23 well-established mothur tools (v.1.36) and an additional 9 custom-made tools that have been integrated and combined in Galaxy , and allows for a fully automated sequence interpretation of 16S rRNA gene micPCR/NGS data.

Article Title: CFTR dysregulation drives active selection of the gut microbiome
Article Snippet: .. 16S rRNA gene amplicon libraries were prepared according to Illumina’s 16S metagenomics sequencing library protocol (Illumina Inc., San Diego, CA). .. Briefly, an approximate 460 bp region within the V3-V4 region of the 16S rRNA gene was amplified by PCR using the forward primer S-D-Bact-0341-b-S-17 and reverse primer S-D-Bact-0785-a-A-21 [ ] with Illumina 5’ overhang adapter sequences: Forward: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’ and Reverse: 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3’.

Article Title: Metagenomic Analysis of a Biphenyl-Degrading Soil Bacterial Consortium Reveals the Metabolic Roles of Specific Populations
Article Snippet: .. Whole-community DNA extracted from the consortium was used to analyze biodiversity by Illumina sequencing of a 16S rRNA gene amplicon library and to determine the metagenome by whole-genome shotgun Illumina sequencing. ..

Article Title: Male spiders control offspring sex ratio through greater production of female-determining sperm
Article Snippet: .. 16S rRNA gene amplicon libraries of variable regions V3 and V4 were prepared according to Illumina's 16S Metagenomic Sequencing Library Preparation guide, using Bac 341F and Bac 805R primers [ ] and sequenced on a MiSeq desktop sequencer (Illumina). .. Sequence analysis, OTU clustering and taxonomic classification was done using mothur v. 1.39.0 [ ], with Silva SSU NR release 128 as a reference [ ].

Article Title: Bacteria Associated With a Commercial Mycorrhizal Inoculum: Community Composition and Multifunctional Activity as Assessed by Illumina Sequencing and Culture-Dependent Tools
Article Snippet: .. Library Preparation Three 16S rRNA gene amplicon libraries were prepared by PCR amplification of an approximate 630 bp region within the hypervariable (V3-V4) region of the 16S rRNA gene according to the Illumina 16S metagenomic sequencing library protocol. .. PCR amplification was performed with broad spectrum 16S rRNA primers (forward primer: 5′-TCGTCGGCAGCGTCAGATGTGTAT AAGAGACAGCCTACGGGNGGCWGCAG-3′, reverse primer: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3′) (Klindworth et al., ), using Kapa HiFi HotStart 2 × ReadyMix DNA polymerase (Kapa Biosystems Ltd., London, UK).

Article Title: Rett Syndrome: A Focus on Gut Microbiota
Article Snippet: .. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). .. In the first-step PCR, 16S rRNA gene of all bacteria was amplified as described by Klindworth et al. [ ].

Polymerase Chain Reaction:

Article Title: Bacteria Associated With a Commercial Mycorrhizal Inoculum: Community Composition and Multifunctional Activity as Assessed by Illumina Sequencing and Culture-Dependent Tools
Article Snippet: .. Library Preparation Three 16S rRNA gene amplicon libraries were prepared by PCR amplification of an approximate 630 bp region within the hypervariable (V3-V4) region of the 16S rRNA gene according to the Illumina 16S metagenomic sequencing library protocol. .. PCR amplification was performed with broad spectrum 16S rRNA primers (forward primer: 5′-TCGTCGGCAGCGTCAGATGTGTAT AAGAGACAGCCTACGGGNGGCWGCAG-3′, reverse primer: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3′) (Klindworth et al., ), using Kapa HiFi HotStart 2 × ReadyMix DNA polymerase (Kapa Biosystems Ltd., London, UK).

Amplification:

Article Title: A Walnut-Enriched Diet Affects Gut Microbiome in Healthy Caucasian Subjects: A Randomized, Controlled Trial
Article Snippet: .. The 16S rRNA gene amplicon libraries were sequenced in paired-end modus using an Illumina MiSeq. ..

Article Title: Monitoring of microbial dynamics in a drinking water distribution system using the culture-free, user-friendly, MYcrobiota platform
Article Snippet: .. FASTQ-formatted sequences were extracted after paired-end sequencing of the 16S rRNA gene amplicon library using the MiniSeq system (Illumina) and processed using our previously developed bioinformatics analysis service . .. This bioinformatics pipeline consists of 23 well-established mothur tools (v.1.36) and an additional 9 custom-made tools that have been integrated and combined in Galaxy , and allows for a fully automated sequence interpretation of 16S rRNA gene micPCR/NGS data.

Article Title: CFTR dysregulation drives active selection of the gut microbiome
Article Snippet: .. 16S rRNA gene amplicon libraries were prepared according to Illumina’s 16S metagenomics sequencing library protocol (Illumina Inc., San Diego, CA). .. Briefly, an approximate 460 bp region within the V3-V4 region of the 16S rRNA gene was amplified by PCR using the forward primer S-D-Bact-0341-b-S-17 and reverse primer S-D-Bact-0785-a-A-21 [ ] with Illumina 5’ overhang adapter sequences: Forward: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’ and Reverse: 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3’.

Article Title: Metagenomic Analysis of a Biphenyl-Degrading Soil Bacterial Consortium Reveals the Metabolic Roles of Specific Populations
Article Snippet: .. Whole-community DNA extracted from the consortium was used to analyze biodiversity by Illumina sequencing of a 16S rRNA gene amplicon library and to determine the metagenome by whole-genome shotgun Illumina sequencing. ..

Article Title: Male spiders control offspring sex ratio through greater production of female-determining sperm
Article Snippet: .. 16S rRNA gene amplicon libraries of variable regions V3 and V4 were prepared according to Illumina's 16S Metagenomic Sequencing Library Preparation guide, using Bac 341F and Bac 805R primers [ ] and sequenced on a MiSeq desktop sequencer (Illumina). .. Sequence analysis, OTU clustering and taxonomic classification was done using mothur v. 1.39.0 [ ], with Silva SSU NR release 128 as a reference [ ].

Article Title: Bacteria Associated With a Commercial Mycorrhizal Inoculum: Community Composition and Multifunctional Activity as Assessed by Illumina Sequencing and Culture-Dependent Tools
Article Snippet: .. Library Preparation Three 16S rRNA gene amplicon libraries were prepared by PCR amplification of an approximate 630 bp region within the hypervariable (V3-V4) region of the 16S rRNA gene according to the Illumina 16S metagenomic sequencing library protocol. .. PCR amplification was performed with broad spectrum 16S rRNA primers (forward primer: 5′-TCGTCGGCAGCGTCAGATGTGTAT AAGAGACAGCCTACGGGNGGCWGCAG-3′, reverse primer: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3′) (Klindworth et al., ), using Kapa HiFi HotStart 2 × ReadyMix DNA polymerase (Kapa Biosystems Ltd., London, UK).

Article Title: Rett Syndrome: A Focus on Gut Microbiota
Article Snippet: .. 16S rRNA gene amplicon libraries were performed with a two-step barcoding approach according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). .. In the first-step PCR, 16S rRNA gene of all bacteria was amplified as described by Klindworth et al. [ ].

BAC Assay:

Article Title: Male spiders control offspring sex ratio through greater production of female-determining sperm
Article Snippet: .. 16S rRNA gene amplicon libraries of variable regions V3 and V4 were prepared according to Illumina's 16S Metagenomic Sequencing Library Preparation guide, using Bac 341F and Bac 805R primers [ ] and sequenced on a MiSeq desktop sequencer (Illumina). .. Sequence analysis, OTU clustering and taxonomic classification was done using mothur v. 1.39.0 [ ], with Silva SSU NR release 128 as a reference [ ].

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  • 91
    Illumina Inc 16s rrna gene amplicon sequencing libraries
    Enterobacteria (A) and Lachnospiraceae (B) <t>16S</t> <t>rRNA</t> gene copies in sow fecal content at beginning and end of gestation. Horizontal bars represent the mean of each group, each point represent the fecal content of a single sow. ∗ Indicates p
    16s Rrna Gene Amplicon Sequencing Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/16s rrna gene amplicon sequencing libraries/product/Illumina Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    16s rrna gene amplicon sequencing libraries - by Bioz Stars, 2020-07
    91/100 stars
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    92
    Illumina Inc community bacterial 16s rrna gene amplicon libraries
    <t>16S</t> <t>rRNA</t> DGGE profiles of advanced IOB enrichments taken from liquid cultures with (+) and without (–) Fe 2+ addition (Step 5 in Figure 2 ) and phylogenetic tree of selected 16S rRNA gene sequences obtained from isolated DGGE bands. The tree was constructed using neighbor-joining method in ARB after 1000 bootstrap iterations. Closely related sequences, with respective GenBank accession numbers, are shown as reference. White, green, and yellow dots are sequences from bands that were solely detected in incubations with Fe 2+ , detected in both incubations with and without Fe 2+ , and solely detected in incubations without Fe 2+ , respectively. Scale bar indicates 10% sequence difference.
    Community Bacterial 16s Rrna Gene Amplicon Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/community bacterial 16s rrna gene amplicon libraries/product/Illumina Inc
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    community bacterial 16s rrna gene amplicon libraries - by Bioz Stars, 2020-07
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    93
    Illumina Inc multiplexed 16s rrna gene amplicon libraries
    Diversity dynamics during idli batter fermentation as determined by <t>16S</t> <t>rRNA</t> gene <t>amplicon</t> sequencing. (a) Family-level and (b) genus-level distribution of the bacterial communities at different time points of the idli batter fermentation. (c) Correlation matrix showing the Pearson’s rank correlation among the top 10 core genera. The Pearson’s rank correlation coefficient ranges from 1 to −1, corresponding to a strongly positive to a strongly negative correlation. (d) Weighted Unifrac PCoA plot highlighting a potential difference in community structure at different intervals of the fermentation process.
    Multiplexed 16s Rrna Gene Amplicon Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiplexed 16s rrna gene amplicon libraries/product/Illumina Inc
    Average 93 stars, based on 1 article reviews
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    multiplexed 16s rrna gene amplicon libraries - by Bioz Stars, 2020-07
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    89
    Illumina Inc 16s rrna gene amplicon library preparation
    Microbial composition of the Ethane50 culture. (A and B) Relative abundance of phylogenetic clades of archaea (A) and bacteria (B) based on <t>16S</t> <t>rRNA</t> gene <t>amplicon</t> sequencing present in the inoculated sediment, and in cultures with no substrate, with methane and ethane after 150 days of incubation. (C) Relative abundance of active microbial groups based on 16 rRNA fragments recruited from the genome of Ethane37 and Ethane50 after 2.5 years of incubation and the transcriptome of the Ethane50 culture after 1 year of incubation with ethane. (D and E) Laser-scanning micrograph (D) and epifluorescence micrograph (E) of microbial consortia stained with probes specific for the GoM-Arc1 clade (red, Alexa 594) and “ Ca. Desulfofervidus” (green, Alexa 488) in the Ethane50 culture. Bar, 10 μm.
    16s Rrna Gene Amplicon Library Preparation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 89/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
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    Image Search Results


    Enterobacteria (A) and Lachnospiraceae (B) 16S rRNA gene copies in sow fecal content at beginning and end of gestation. Horizontal bars represent the mean of each group, each point represent the fecal content of a single sow. ∗ Indicates p

    Journal: Frontiers in Microbiology

    Article Title: Reduction of Salmonella Shedding by Sows during Gestation in Relation to Its Fecal Microbiome

    doi: 10.3389/fmicb.2017.02219

    Figure Lengend Snippet: Enterobacteria (A) and Lachnospiraceae (B) 16S rRNA gene copies in sow fecal content at beginning and end of gestation. Horizontal bars represent the mean of each group, each point represent the fecal content of a single sow. ∗ Indicates p

    Article Snippet: 16S rRNA gene amplicon sequencing libraries were prepared following the Illumina MiSeq protocol ( ).

    Techniques:

    16S rRNA DGGE profiles of advanced IOB enrichments taken from liquid cultures with (+) and without (–) Fe 2+ addition (Step 5 in Figure 2 ) and phylogenetic tree of selected 16S rRNA gene sequences obtained from isolated DGGE bands. The tree was constructed using neighbor-joining method in ARB after 1000 bootstrap iterations. Closely related sequences, with respective GenBank accession numbers, are shown as reference. White, green, and yellow dots are sequences from bands that were solely detected in incubations with Fe 2+ , detected in both incubations with and without Fe 2+ , and solely detected in incubations without Fe 2+ , respectively. Scale bar indicates 10% sequence difference.

    Journal: Frontiers in Microbiology

    Article Title: Diversity of Iron Oxidizers in Groundwater-Fed Rapid Sand Filters: Evidence of Fe(II)-Dependent Growth by Curvibacter and Undibacterium spp.

    doi: 10.3389/fmicb.2018.02808

    Figure Lengend Snippet: 16S rRNA DGGE profiles of advanced IOB enrichments taken from liquid cultures with (+) and without (–) Fe 2+ addition (Step 5 in Figure 2 ) and phylogenetic tree of selected 16S rRNA gene sequences obtained from isolated DGGE bands. The tree was constructed using neighbor-joining method in ARB after 1000 bootstrap iterations. Closely related sequences, with respective GenBank accession numbers, are shown as reference. White, green, and yellow dots are sequences from bands that were solely detected in incubations with Fe 2+ , detected in both incubations with and without Fe 2+ , and solely detected in incubations without Fe 2+ , respectively. Scale bar indicates 10% sequence difference.

    Article Snippet: Finally, the relative abundance of the newly identified and well-known IOB in the full-scale RSFs was assessed by inspecting the community bacterial 16S rRNA gene amplicon libraries (Illumina MiSeq and 454) against FISH-verified full 16S rRNA clones from IOB enrichments.

    Techniques: Denaturing Gradient Gel Electrophoresis, Isolation, Construct, Sequencing

    Phylogenetic trees of bacterial 16S rRNA gene sequences retrieved from incubations with Fe(II) addition after 15 days incubation (Numbers as in Figure 3 ; see Figure 2 step 5). The tree was constructed using neighbor-joining method in ARB from full length sequences obtained via cloning and partial sequences (170 bp) obtained from excised DGGE bands. Percentages in parentheses correspond to the relative abundance of clones with identical ARDRA profiles; remaining clones represented as single OTU in corresponding ARDRA profiles. Thick branches on phylogenetic trees indicate high parsimony bootstrap support (≥70%) based on 1000 iterations. Scale bars indicate 10% sequence difference.

    Journal: Frontiers in Microbiology

    Article Title: Diversity of Iron Oxidizers in Groundwater-Fed Rapid Sand Filters: Evidence of Fe(II)-Dependent Growth by Curvibacter and Undibacterium spp.

    doi: 10.3389/fmicb.2018.02808

    Figure Lengend Snippet: Phylogenetic trees of bacterial 16S rRNA gene sequences retrieved from incubations with Fe(II) addition after 15 days incubation (Numbers as in Figure 3 ; see Figure 2 step 5). The tree was constructed using neighbor-joining method in ARB from full length sequences obtained via cloning and partial sequences (170 bp) obtained from excised DGGE bands. Percentages in parentheses correspond to the relative abundance of clones with identical ARDRA profiles; remaining clones represented as single OTU in corresponding ARDRA profiles. Thick branches on phylogenetic trees indicate high parsimony bootstrap support (≥70%) based on 1000 iterations. Scale bars indicate 10% sequence difference.

    Article Snippet: Finally, the relative abundance of the newly identified and well-known IOB in the full-scale RSFs was assessed by inspecting the community bacterial 16S rRNA gene amplicon libraries (Illumina MiSeq and 454) against FISH-verified full 16S rRNA clones from IOB enrichments.

    Techniques: Incubation, Construct, Clone Assay, Denaturing Gradient Gel Electrophoresis, Sequencing

    Diversity dynamics during idli batter fermentation as determined by 16S rRNA gene amplicon sequencing. (a) Family-level and (b) genus-level distribution of the bacterial communities at different time points of the idli batter fermentation. (c) Correlation matrix showing the Pearson’s rank correlation among the top 10 core genera. The Pearson’s rank correlation coefficient ranges from 1 to −1, corresponding to a strongly positive to a strongly negative correlation. (d) Weighted Unifrac PCoA plot highlighting a potential difference in community structure at different intervals of the fermentation process.

    Journal: Applied and Environmental Microbiology

    Article Title: Diversity and Succession of Microbiota during Fermentation of the Traditional Indian Food Idli

    doi: 10.1128/AEM.00368-19

    Figure Lengend Snippet: Diversity dynamics during idli batter fermentation as determined by 16S rRNA gene amplicon sequencing. (a) Family-level and (b) genus-level distribution of the bacterial communities at different time points of the idli batter fermentation. (c) Correlation matrix showing the Pearson’s rank correlation among the top 10 core genera. The Pearson’s rank correlation coefficient ranges from 1 to −1, corresponding to a strongly positive to a strongly negative correlation. (d) Weighted Unifrac PCoA plot highlighting a potential difference in community structure at different intervals of the fermentation process.

    Article Snippet: The sequencing of multiplexed 16S rRNA gene amplicon libraries was performed using paired-end 2 × 150-bp chemistry on the Illumina MiSeq platform.

    Techniques: Amplification, Sequencing

    Microbial composition of the Ethane50 culture. (A and B) Relative abundance of phylogenetic clades of archaea (A) and bacteria (B) based on 16S rRNA gene amplicon sequencing present in the inoculated sediment, and in cultures with no substrate, with methane and ethane after 150 days of incubation. (C) Relative abundance of active microbial groups based on 16 rRNA fragments recruited from the genome of Ethane37 and Ethane50 after 2.5 years of incubation and the transcriptome of the Ethane50 culture after 1 year of incubation with ethane. (D and E) Laser-scanning micrograph (D) and epifluorescence micrograph (E) of microbial consortia stained with probes specific for the GoM-Arc1 clade (red, Alexa 594) and “ Ca. Desulfofervidus” (green, Alexa 488) in the Ethane50 culture. Bar, 10 μm.

    Journal: mBio

    Article Title: “Candidatus Ethanoperedens,” a Thermophilic Genus of Archaea Mediating the Anaerobic Oxidation of Ethane

    doi: 10.1128/mBio.00600-20

    Figure Lengend Snippet: Microbial composition of the Ethane50 culture. (A and B) Relative abundance of phylogenetic clades of archaea (A) and bacteria (B) based on 16S rRNA gene amplicon sequencing present in the inoculated sediment, and in cultures with no substrate, with methane and ethane after 150 days of incubation. (C) Relative abundance of active microbial groups based on 16 rRNA fragments recruited from the genome of Ethane37 and Ethane50 after 2.5 years of incubation and the transcriptome of the Ethane50 culture after 1 year of incubation with ethane. (D and E) Laser-scanning micrograph (D) and epifluorescence micrograph (E) of microbial consortia stained with probes specific for the GoM-Arc1 clade (red, Alexa 594) and “ Ca. Desulfofervidus” (green, Alexa 488) in the Ethane50 culture. Bar, 10 μm.

    Article Snippet: Two nanograms of DNA was used for amplicon PCR, and the product was used for 16S rRNA gene amplicon library preparation according to the 16S metagenomic sequencing library preparation guide provided by Illumina.

    Techniques: Amplification, Sequencing, Incubation, Staining